简介：Objective:Toanalyzetheprinciplemechanismofthearcusplantarisanditsclinicalapplication.Methods:Thestatesofforcessustainedbythearcusplantariswereanalyzedandcalculatedaccordingtothemechanismofthequadraticparabolicarch.Results:Theaponeurosisplantariscorrespondedtothepullrodofthearcusplantaris.Themedialandlaterallongitudinalarchesformedbythepedalboneswerestablewiththerod,butunstablewithouttherod.Inthelattercondition,onloading,theforcesustaingedbytheparabolicarchbecameaforcesustainedbyasimplebeam,andthearcusplantaristendedtodisappear5andtobeflattened.clinically,240feetwithtalipesequinusweretreatedwithtriplearthrodesis.In34outofthereexamined156feet,theaponeurosisplantariswascutinadditiontothetriplearthrodesisandwasimmobilizedwithcastfor3months.Oneortwoyearslater,theirarcusplantarisdisappeared,paindevelopedwhenwalking,andsomeofthemwalkedwiththemidtarsaljointagainsttheground.Then,thetriplearthrodesisandshorteningoftheaponeurosisplantariswereappliedon18cases,andosteotomyofthecalcaneusandreconstructionoftheaponeurosisplantarisweremadeon10casesandsatisfactoryeffectswereobtained.Conclusions:Inordertoachievesatisfactorytherapeuticeffectsofthetriplearthrodesis,weshouldreestablishthearcusplantarisandaccuratelytreattheaponeurosisplantarisforthebalanceofthesurroundingmuscleforce.

简介：Objective:Toprovidedetailedinformationofcoronamortisforilioinguinalapproachasananteriorapproachtotheacetabulumandpelvis.Methods:Thecourse,branchesanddistributionofthevascularconnectionbetweentheobturatorsystemandtheexternaliliacorinferiorepigastricsystemslocatedoverthesuperiorpubicramuswereobservedon50hemipelviseswithintactsofttissues.Results:Duringthedissections,72%ofthecadavericsideshadatleastonecommunicatingvesselbetweentheobturatorsystemandtheexternaliliacorinferiorepigastricsystemsonthesuperiorpubicramus.Theaveragediameteroftheconnectingvesselwas2.6mm(range,2.0-4.2mm).Itcoursedoverthesuperiorpubicramusoriliopubiceminenceverticallytoentertheobturatorforamenandexitthepelvis.Theaveragedistancefrompubicsymphysistothevascularconnectionsbetweentheobturatorandexternaliliacsystemswas52mm(range,38-68mm).Conclusions:Vascularconnectionsbetweentheobturatorsystemandtheexternaliliacorinferiorepigastricsystemswerefoundoverthesuperiorpubicramuswithahighincidence.Theyarepronetodamageduringtheilioinguinalapproachasananteriorapproachtotheacetabulumandpelvis.Thus,coronamortislocatedoverthesuperiorpubicramusdeservesgreatattentionduringtheilioinguinalapproach.

简介：ＣＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｏｆＳｕｒｇｅｒｙ，ＤａｐｉｎｇＨｏｓｐｉｔａｌ，ＴｈｉｒｄＭｉｌｉｔａｒｙＭｅｄｉｃａｌＵｎｉｖｅｒｓｉｔｙ，Ｃｈｏｎｇｑｉｎｇ４０００４２，Ｃｈｉｎａ（ＬｉｕＢＳ，ＷａｎｇＺＧ，ＷｅｎｇＧＷ，ＹａｎｇＺＨ，Ｌ...

简介：Objective:Toprovideanatomicalbasisfortheinternalfixationofscaphoidfractures.Methods:Theshapeandvascularlakeof48dryscaphoidsand36wetscaphoidswereobserved.Results:Thedataofdrybonegroupandwetbonegroupwereasfollows:theheightofscaphoidtuberclewere(11.28±0.94)mmand(10.35±1.54)mm;thethicknessofscapoidwaistwere(12.02±1.90)mmand(11.21±1.20)mm;thewidthofscapoidwaistwere(10.59±1.11)mmand(11.34±1.47)mm;theminimalthicknessofthebodyofscapoidwere(6.51±1.22)mmand(8.54±1.07)mm;theaxislengthofscapoidwere(25.68±2.21)mmand(26.50±2.56)mm;thewidthofepicondyleofscaphoidofdistalportion,waistandproximalportionwere(6.50±1.06)mm,(5.14±1.01)nun,(4.42±1.16)mmand(6.64±1.18)mm,(6.01±0.75)mmand(5.71±0.78)mm,respestively.Themainbloodvesselscamefromthedorsalandtheradialofwristandpassedthroughthewholescaphoidalongthecrestofscaphoid.Conclusion:Theinternalfixationofscaphoidcanbedesignedaccordingtotheanatomicaldatawithoutdamag-ingthearticularsurfaceandbloodsupply.

简介：RecentstudiessuggestthatMHCclassⅡgenetransfercouldstimulateprotectiveantitumorimmunity.WhetherxenogeneicMHCⅡtransfectedtumorcellshavetheseeffectisunknown.Inthisstudy,HLA-DR3αandβchaincDNAwereclonedbyRT-PCRfromhumanBlymphomaRajicelllineandconfirmedbyDNAsequencing.TherecombinantexpressingvectorswereconstructedbyinsertingtheαandβchaincDNAintoPCEP4/pLXSNvectorsrespectively.AftertransfectingbylipofectamineandselectingwithG418andHyg,theHLA-DR3transfectedmousemelanomaB16recombinantcloneswereobtained.Approximately59%ofthe

简介：目的将与traumatic以后癫痫观察在病人的生活的质量并且讨论影响因素。我们用生活Scale-31(QOL-31)的质量作为控制与traumatic以后癫痫和100个健康的人估计了105个病人，并且在消沉上进行了回顾的分析的方法，自我等级压抑规模(SDS)和自我等级焦虑规模(SAS)，焦虑，损伤的地点，抓住的控制，EEG和治疗学的依从。有癫痫获得了的traumatic以后的结果病人很在QOL-31上比控制组降低(P<0.01)，但是比SDS和SAS上的控制组高(P<0.01)。多重回归分析显示生活的质量上的主要影响因素是焦虑，治疗学的依从，消沉，损伤的癫痫的抓住和地点的差的控制。在有posttraumatic癫痫的病人的生活的质量显著地衰退了的结论。医生们应该与癫痫注意病人的心理、心理的问题，例如消沉和焦虑，提高治疗学的依从并且控制癫痫的抓住，它是改进预后的关键。

简介：客观：在山羊胫节在外皮的骨头灌注上把点contact-dynamiccompression板(PC-DCP)和它的效果的机械性质与动态压缩盘子(DCP)的作比较。方法：二十匹配的新鲜山羊胫节被使用。一个横向的破裂模型被建立。有在骨折结束之间的3mm间隙星际的破裂分别地与DCP和PC-DCPs服从于固定，然后，thefour指弯曲测试和扭转测试被进行把PC-DCP的机械性质与DCP的作比较。另一13只性成熟的山羊与DCPsand经历了固定PC-DCPs，分别地在未经触动的双边的胫节中间柄。Ischemic地区在四个次点被观察(1天，在操作以后的2,6，和12个星期)用disulphine蓝染色技术。结果：在机械性质没有重要差别，例如拐弯抵抗和扭转抵抗，在DCP和PC-DCPs之间。有天，在操作以后的2，和6个星期在DCP固定的方面上，在坚持的板下面的外部外皮的骨头局部缺血，和这个条件没在操作以后逆行直到12个星期。在PC-DCP固定的方面上，然而，外皮的骨头局部缺血仅仅在螺丝钉洞并且在PC坚果的接触地点的圆周发生了1天在操作，和它在操作以后消失在2个星期以后。结论：PC-DCP有类似的简历DCP的机械性质，但是比常规盘子对本地骨头血循环不太有害。

简介：Objective:Toobservetherevascularizationandtheopportunityofcross-fingerflap.Methods:Ananimalmodelwasdevelopedtopermitdailymonitoringofneovascularizationoftheflapwithautoradiography,tissuetransparenttechnique,grossobservationandhistologicalexamination.Results:Therevascularizationoftheflapwaschieflyraisedfromthesurroundingtissues.Thepediclesof334cross-fingerflapsofthepatientsweresuccessfullydividedfrom1to5dafteroperations,averagely3.3d.Allofthecasesshowedsatisfatoryresultsaccordingtoafollow-upsurveyof3to72mon.theresultsfurtherprovedthattherevascularizationofthecross-fingerflapshadaccomplishedwithin3d.Conclusions:Itsuggeststhatthedivisionofacrossfingerflapcanbecarriedoutbetweenthe3rdand5thdayafteroperationwithahighdegreeofsafety.

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简介：Objective:TostudythesequentialchangesofHIF-1α(hypoxia-induciblefactor1alpha)inexperimentalspinalcordinjuryinratsandtoanalyzeitspotentialeffectsinSCI.Methods:AstaticcompressionmodelofSCIwasemployedinthisstudy.ExpressionsofHIF-1αweremeasuredwithimmunohistochemicalstaining,whileflowcytometrywasusedtodeterminetheapoptoticratioandbcl-2expressions.Results:HIF-1αbegantoincrease1dayafterinjury,andreachedthepeakat3-7days.Twoweekslater,itdeclinedsignificantly.ThesequentialchangesofHIF-1αcoincidedwellwiththealterationsofapoptoticratioandcontentsofbel-2.Conclusions:HIF-1αpossiblyparticipatesinthesecondaryischemicandhypoxicproceduresafterspinalcordinjury,andmaymediatethetraumaticapoptosis.FurtherunderstandingofHIF-1αmayprovidenewtherapeuticregimensforSCI.

简介：Objective:Toevaluatetheprotectiveeffectsof8%emulsifiedisofluraneaftermyocardialischemia-reperfusioninjuryanditsmechanisminrabbits.Methods:Twenty-fourmaleadultNewZealandwhiterabbitswereanesthetizedwithintravenousinjectionof30mg/kgpentobarbitalfollowedby5mg·kg-1·h-1infusion.Allrabbitsweresubjectedto30minutesofleftanteriorde-scendingcoronaryartery(LAD)occlusionand3hoursofsubsequentreperfusion.BeforeLADocclusion,therabbitswererandomlyallocatedintothreegroupsforprecondi-tioningtreatment(eightforeachgroup).Thecontrolgroup(Cgroup)receivedintravenously0.9%NaClfor30minutes.Theemulsifiedisofluranegroup(EIgroup)received8%emulsifiedisofluraneintravenouslytill0.64%end-tidalcon-centrationfor30minutesthatwasfollowedbya15-minutewashoutperiod.TheIntralipidgroup(INgroup)received30%Intralipidfor30minutes.Theinfarctedarea,plasmamalondialdehyde(MDA)content,superoxidedismutaseactivity(SOD)andnitriteconcentrationafter3-hourmyo-cardialperfusionwererecordedsimultaneously.Results:Forthemyocardialischemia-reperfusionin-juryanimals,theinfarctedsizeintheEIgroupwassignifi-cantlyreduced(91.9%±8%)ascomparedwithcontrolgroup(39%±6%,t=5.19,P<0.01).TheplasmaSODactivityandnitriteconcentrationinEIgroupweresignificantlyhigherthanthoseincontrolgroup(t=2.82,t=8.46,P<0.05),butMDAcontentwaslowerinEIgroupthanthatincontrolgroup(t=2.56,P<0.05).Conclusions:Theresultsindicatethatemulsifiedisofluranehasacardioprotectioneffectagainstischemia-reperfusioninjury.ThisbeneficialeffectofemulsifiedisofluraneisprobablythroughNOreleaseandconsequentlybyincreaseinautioxidationofmyocardium.

简介：Objective:ToinvestigatetheeffectofsubstanceP(SP)ongeneexpressionoftransforminggrowthfactorβ-1(TGFβ-1),transforminggrowthfactorreceptor-1(TGFR-1)andtransforminggrowthfactorreceptor-2(TGFR-2)infibroblastsculturedinvitrofromrat'sgranulationtissues.Methods:Thefibroblastsfromthegranulationtissuesintheskeletalmuscleofrat'shindlimbsinjuredbyformaldehydewereculturedinvitro.Whendifferentconcentrations(10-9-10-5mol/L)ofSPwereaddedintotheculturemedium,thechangesofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2intheculturedfibroblastswereobservedwithreversetranscriptionpolymerasechainreactionatdifferentintervals(0,3,6,12and24hoursafterincubation).Results:ThegeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblastsculturedfromrat'sgranulationtissueswasup-regulatedbySP.ThepeaklevelofthemRNAexpressionwasfoundat10-8mol/LSPandtheup-regulationeffectwasnotfoundat10-5mol/Land10-6mol/L.ThepeaklevelsofgeneexpressionofTGFβ-1,TGFR-1andTGFR-2inthefibroblaststreatedwithSPwereachievedat6and12hours,respectively.Conclusions:SPhasup-regulationeffectonthegeneexpressionofTGFβ-1,TGFR-1andTGFR-2infibroblastsfromrat'sgranulationtissuesinvitro,andtheeffectisrelatedtodifferentstimulatingconcentrationsofSP.Itmaybeconcernedwithproliferationanddifferentiationoffibroblastsandformationofscartissuesduringwoundhealing.

简介：Objective:ToanalyzethedynamicchangeofserumproteinS100binpatientswithtraumaticbraininjuryanditsclinicalvalueinassessingbraindamage.Methods:AccordingtoGlasgowcomascale(GCS),102casesoftraumaticbraininjuryweredividedintomildbraininjurygroup(GCS≥13,n=31,GroupA),moderatebraininjurygroup(8

简介：ToexploretherelationshipbetweensubstanceP(SP)releasedfromperipheralnerveendingsandtheexpressionofepidermalgrowthfactor(EGF)andepidermalgrowthfactorreceptor(EGFR)duringwoundhealing.Methods:FiftyWistarratswererandomlydividedinto2groups,injurygroupandcapsaicingroup.Intheinjurygroup,afull-thicknessskinwoundonthebackoftheratwastaken.Thewoundedgeandgranulationtissuesweretakenonthe1st,3rd,6th,9th,12thdaysafterinjury,respectively.Inthecapsaicingroup,capsaicinwasinjectedsubcutaneouslyonthebackoftheratstodestroythesensorynervetopreventthesecretionofSP,thenawoundandsamplewasmadeinthesameway.ImmunohistochemistryandinsituhybridizationwereemployedtodetecttheexpressionofSP,EGF/EGFR,andEGFmRNA/EGFRmRNAinthegranulationtissues.Results:Intheinjurygroup,immunohistochemicalstainofSPandEGF/EGFRwaslocatedonthehairfolliclesandsebaceousglandsatthe1stday.AndthestainofSPwasobviousatthe3rddayinthegranulationtissues,thendecreasedgradually.EGF/EGFRwasatlowlevelatthe3rdday,thenincreasedgraduallyandreachedthepeakatthe9thday,thendeclined.Inthecapsaicingroup,theimmunohistochemicalstainofSPandEGF/EGFRwasfaintandwithoutobviouschangeduringthewoundhealingprocess.ThetendencyoftheEGFmRNA/EGFRmRNAexpressionwassimilartothatofEGF/EGFR.Conclusions:Duringwoundhealing,SPmaypromotethehealingprocessbyaffectingtheexpressionofEGF/EGFRinthegranuationtissues.

简介：Objective:Toinvestigatetheexpressionandpatternofvascularendothelialgrowthfactor(VEGF)anditsfetalliverkinase-1(Flk-1)receptorinspinalcordanddorsalrootgangliaafterneurotomyofsciaticnerveinrats.Methods:Forty-fiveadultmaleWistarratsweredividedrandomlyintoacontrolgroup(n=5)andanexperimentalgroup(n=40).ThebilateralsciaticnervesoftheratsintheexperimentalgroupunderwentneurotomyandtheL4-L6spinalcordandthecorrespondingdorsalrootgangliawereharvestedrespectivelyat8hours,and1,3,5,7,10,14and21days(8subgroupswith5ratseach)afteroperation.Theratsinthecontrolgrouponlyunderwentanexposureofsciaticnervewithoutneurotomy.ImmunohistochemistryandimageanalysiswereusedtostudytheexpressionofVEGFanditsFlk-1receptor.Results:BothVEGFandFlk-1receptorexpressedinthenormalratspinalcordanddorsalrootganglia.Inresponsetoneurotomy,theirexpressionreachedahigherlevelandpersistedforashorttimethendeclinedtothenormallevelrapidly.Besides,positivestainingofFlk-1wasobservedinbothglialcellsandnervefibers,whichlocatedinthewhitematterofthespinalcord.Conclusions:VEGFcanpromotetheregenerationofperipheralnervesfromtheangleofcentralneurons,whichestablishestheexperimentalandtheoreticalfoundationforVEGFtreatingperipheralnerveinjuries.