简介：Objective:Toinvestigatetheroleandclinicalsignificanceofp16proteininCondylomaAcuminatum(CA)anditscancerization.Methods:Theexpressionofp16proteinwastestedin33CAsamplesand7cancerizedCAsamplesbyimmunohistochemicalassays.Results:Therewasabnormalexpressionofp16proteininCAandcancerizedCA,mainlymajorproteinexpression.Thep16proteinexpresseedindifferentlocationsindifferentcaseswasasfollows:Inbasallayercellsinnormalcuits;inspinouslayer,granularlayerandstratumcorneumlayercellsinCA;inkeratinpearlperipheralandspinouslayercellsincancerizedCA.Conclusion..Therewasmajorexpressionofp16proteininCAandcancerizedCA,andtheseproteinofthetwogroupsmightnotnaturallybethesame.Ourstudyindicatedthatinclinicalpractice,whenmajorp16proteinexpressioninCAoccurs,it'sriskofcancerizationshoudbesuspected.

简介：Objective:ToanalyzeHIVreplicationandimmunefunctionchangesamongChinesepatientswithHIV/ARC/AIDSandtheirassociationwithdiseaseprogression.Methods:Tcellsubsets,plasmacytokineconcentrationsandviralloadsfrom42HIV+individuals,and13ARC/AIDSpatients,andI0controlswereanalyzedbyflowcytometry(FCM),quantitativeELISAandreversetranscription-polymerasechainreaction(RT-PCR),respectively.Results:CD4cellcountsandplasmaIL-2inHIV/AIDSpatientsweresignificantlylessthaninnormalcontrolsubjects(P<0.001).TheplasmaconcentrationsslL-2R,TNF-a,andNeopterinincreasedsignificantlywithdecreasingCD4cellcounts.PlasmaIL-2amongAIDS/ARCpatientswasalsolessthaninHIV+patients(P<0.01).CD4cellcounts,theratioofCD4toCD8andplasmaIL-2levelsdecreasedsignificantlywithinfectionduration.CD4cellcountsdeclinedanaverageof43/miperyear.Incontrast,theconcentrationofplasmaslL-2R,sTNFR-I,andNeopterinincreasedanaverageof9.03pg/ml,8.69pg/ml,2.11ng/mlperyear,respectively.Furthermore,asignificantreverselinearcorrelationwasobservedbetweenCD4cellcount,CD4/CD8ratio,andCD3,CD4andCD8countswithplasmalevelsofsTNFR-I,Neopterin,andHIVRNAload.ApositivelinearcorrelationwasnotedbetweenplasmasIL-2RlevelsandchangesofplasmaIL-6level(P<0.001),IL-10(P<0.001),TNF-a(P<0.001),sTNFR-I(P<0.005),andNeopterin(P<0.002)andbetweenIL-6andTNF-a(P<0.001),NeopterinandIL-10(P<0.05),sTNFR-I(P<0.001),plasmaviralloadandsTNFR-I(P<0.001),andNeopterin(P<0.002).Conclusion:ThesefindingssuggestacloserelationshipbetweenIL-6andTNF-almmuneactivation,HIVreplicationanddiseaseprogressioninprimaryHIVinfectionsandAIDSpatients.DecliningCD4cellsandplasmaIL-2,andincreasingviralload,sIL-2R,TNF-a,sTNFR-I,andNeopterinmightbeconsideredasimportantpredictorsoftheprogressionofHIVinfectiontoAIDS.

简介：Objective:ToconstructtherecombinantplasmidcontainingGlycerophosphodiesterphosphodiesterase(Gpd)genefromTreponemapallidumandtransfectitintoHelacellstoexpresstheencodedoutermembraneprotein.Methods:TheGpdgenewasamplifiedfromthegenomicDNAofT.pallidumbypolymerasechainreaction(PCR)andinsertedintocloningvectorpUCm-T.TheinsertedGpdgenewassubclonedintotheappropriatesiteofpcDNA3.1(+)vector.Afteridentificationbysequencingandrestrictiveenzymesdigestion,therecombinantplasmidwastransfectedintoHelacellsusingliposomes.TheexpressedproteinwasidentifiedbyimmunocytochemistryandWesternblot.Results:ThetargetGpdgenesegmentwasapproximately1059bp.TheDNAsequenceoftheGpdgenecontainedinthepcDNA3.1(+)vectorwasconsistentwiththepublishednucleotidesequence.ThehomologyofthenucleotideandputativeaminoacidsequencesoftheGpdgenebetweenT.pallidumsubsp.pallidumNicholsandvariouspathogenictreponemalstrainsrangedfrom98%to100%.ImmunocytochemistryandWesternblotanalysisshowedthattheconstructedGpd-pcDNA3.1(+)vectorexpressedafusionproteinwithacalculatedmolecularmassof41KDainHelacellsandthattheexpressedproteinreactedwiththeserafromsyphilispatients.Conclusion:ThesuccessfulconstructionandexpressionoftheeukaryoticexpressionplasmidoftheGpdgenefromT.pallidumprovideapromisingtooltofurtherstudythebiologicalactivityofT.pallidumanddevelopaDNAvaccineforsyphilis.