简介：Thelight-harvestingchlorophylla/b-proteincomplexplaysanimportantroleinphotosynthesisofplants.Afull-lengthcDNAoflight-harvestingchlorophylla/b(cab)genewasclonedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRandRACEmethods,namedascab-PhE10(cabgene10fromPh.edulis).Thelengthofcab-PhE10(GenBankaccessionnumber:EU118754)is1151bp,whichcontainsanopenreadingframeencoding283aminoacidsfrom81stto932ndposition.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE10hadachlorophlla/bbindingdomain(83rd-247thposition),twoproteinkinaseC-phosphorylationsites,threeN-myristoylationsitesandayiaA/Bdoublehelixdomain.Theaminoacidsequenceofcab-PhE10showedhighsimilaritywiththecabgenesofOryzasativa,Zeamays,Hordeumvulgare,andVitisvinifera,morethan80%,respectively,whichindicatedthatcab-PhE10genebelongstolhcb5genefamily.

简介：在顺序地层学和litho-paleogeography的学习，定量分析，精确计算和巨大的地质的数据的详细比较例如领域侧面，从不同区域记载记录和地震曲线，是基本要求。为了获得可靠、精确的更多，结果，这份报纸论述把空间数据库分析与单个因素的印射技术相结合建立的一个新奇方法定序地层学的继任并且印射Ordos盆的奥陶纪litho-paleogeography，在诺思中国忍受盆的最大的油气体之一平台。由使用这个方法，所有相关基本地质的数据能是分析并且有效地设法的份量上。基本stratigraphic单位和他们的人物的各种各样的属性penecontemporaneousdolostone内容，浅水粗涂内容，和源自陆地的材料例如顺序厚度，满足，能充分在外形分析并且在印射统计上被利用。把分析基于这，这份报纸被施加了为每奥陶纪印射份量上的单个因素的isopachous在盆，和最后综合的多重因素定序重建为每顺序间隔的litho-paleogeography。学习证明建议方法是相当有效的并且在与传统的方法相比认出litho-paleogeographic子单元有一个高得多的决定。在Ordos盆的中间的奥陶纪顺序间隔(在更低的Majiagou形成的SQ19)之一，例如，作者成功地开发了一个数学公式实质地划分各种各样的外形单位的分发，例如旧陆地，潜水艇高举，supratidal地区，intertidal地区和subtidal地区。

简介：6月6日,由浙江省美术家协会、浙江画院、《FOSPEL·佳音》杂志主办,国际著名策展人W.Y.Choy和C.K.Kum携手策展的＂INSEQUENCE转序＂水墨中国当代艺术展在浙江美术馆举办。

简介：Humanleukocyteantigen(HLA)systemisthemostpolymorphicregionknowninthehumangenome.Inthepresentstudy,weanalyzedforthefirsttimetheHLA-Agenepolymorphismsdefinedbythehigh-resolutiontypingmethods-sequence-basedtyping(SBT)in161NorthernChineseHanpeople.Atotalof74differentHLA-Agenetypesand36allelesweredetected.ThemostfrequentalleleswereA110101(GF=0.2360)，A24020101（GF=0.1646），andA020101(GF=0.1553)；followedbyA3303(GF=0.1180)，A3001（GF=0.0590)，andA310102(GF=0.0404).Thefrequenciesoffollowingalleles,A0203,A0205,A0206,A0207,A030101,A2423,A2601,A3201,andA3301,areallhigherthan0.0093.ThehomozygousallelesincludeA020101,A110101,A24020101andA310102.Heterozygosity(H),polymorphisminformationcontent(PIC),discriminationpower(DP)andprobabilityofpaternityexclusion(PPE)ofHLA-Ainthesampleswerecalculatedandtheirvalueswere0.8705，0.8491，0.6014，and0.9475，respectively.Hanpopulation，especiallytheallelesubtypescharacter,willbeofgreatinterestforclinicaltransplantation,disease-associatedstudyandforensicidentification.Implementationofhigh-resolutiontypingmethodsallowsasignificantlywiderspectrumofHLAvariationincludingrarealleles.Thisrpectrumwillfurtherbeextensivelyutilizedinmanyfields.

简介：在活跃裂缝盆，tectonism为顺序stratigraphic模式是极其重要的，影响顺序建筑学和内部化妆。在Qiongdongnan盆的Paleogene裂缝继任的顺序stratigraphic框架，北华南海，用地震侧面被造，由井木头和核心补充了。一阶的和三个秒顺序序列根据盆规模不顺从被识别，并且七个第三顺序的序列被不顺从在中央盆以内沿着盆边缘和关联词一致定义。通过不顺从分析和backstripping过程，Qiongdongnan盆的深海的区域的构造进化被证明阵发性的Paleogenesynrift，能被划分成rifting舞台，我，riftingstage-II和riftingstage-III。阵发性的rifting导致了结构的斜坡裂缝带的各种各样的类型的形成，它控制了不同体系结构和序列的内部化妆。这研究在顺序stratigraphic模式上提高构造进化的控制的理解并且在典型裂缝盆建立相关模式，并且进一步建议在不同顺序stratigraphic模式发展的有利沙岩水库，它将对在含石油的盆的深海的区域的微妙的水池探索相当有用。

简介：EarthquakesofM_S5.6andM_S6.1occurredinYingjiang,YunnanonMay24andMay30,2014respectively.Inthispaper,weusethewaveformdatarecordedbymobileseismicstations(KAC)whichweresetupinthesourceareaaftertheYingjiangMS5.6earthquakeonMay24,2014tostudytheshear-wavesplittingcharacteristicsofYingjiangM_S6.1earthquakesequencewiththeSAMmethod.Theresultshowsthatpredominantpolarizationoffastshear-wavesbeforetheM_S6.1earthquakeisconsistentwiththedirectionofregionalprincipalcompressivestress,andpredominantpolarizationoffastshear-wavesbeforetheM_S6.1earthquakeshowbetterconsistencyandsmallerdispersioncomparedtoaftertheM_S6.1earthquake,andtheremaybeadeflectionforthefastshearwavespredominantpolarizationbetweentheM_S6.1earthquakesequenceandforeshocksequence.Wefoundthatthetime-delaysgenerallyexhibitedalowerlevelbeforetheM_S6.1earthquakeandarelativelyhighervalueaftertheM_S6.1earthquake.

简介：Wereportacompletegenomicsequenceofrareisolates(minorgenotype)oftheSARS-CoVfromSARSpatientsinGuangdong,China,wherethefirstfewcasesemerged.Themoststrikingdiscoveryfromtheisolateisanextra29-nucleotidesequencelocatedatthenucleotidepositionsbetween27,863and27,864(referredtothecompletesequenceofBJ01)withinanoverlappedregioncomposedofBGI-PUP5(BGI-postulateduncharacterizedprotein5)andBGI-PUP6upstreamoftheN(nucleocapsid)protein.Thediscoveryofthisminorgenotype,GD-Ins29,suggestsasignificantgeneticeventanddifferentiatesitfromthepreviouslyre-portedgenotype,thedominantformamongallsequencedSARS-CoVisolates.A17-ntsegmentofthisextrasequenceisidenticaltoasegmentofthesamesizeintwohumanmRNAsequencesthatmayinterferewithviralgenomereplicationandtranscriptioninthecytosoloftheinfectedcells.Itprovidesanewavenuefortheexplorationofthevirus-hostinteractioninviralevolution,hostpathogenesis,andvaccinedevelopment.

简介：在Upper-Yangtze区域，特别在贵州省和它的邻近的区域，更低寒武纪很好被开发并且由从盆外形的黑页岩的继任是显著的焦化站台外形的岩石。从Sinian发生在拐弯到的站台的淹死的事件寒武纪导致了一套黑页岩，即Niutitang形成，它完成底部部分更低寒武纪。与沉积环境变浅，一套碳酸盐摇，即Qingxudong形成，在最高的部分被形成更低寒武纪。因此，更低在学习区域寒武纪使能进一步的一个秒顺序序列起来细分进五个第三顺序的序列，并且形成违反回归的定期周期的继任。为在秒顺序顺序的第三顺序的序列有一个定期垂直的叠模式。从底部到顶，继任“CS(压缩的节)+HST(高看台的系统道)”第三顺序的序列被变成继任“TST(transgressive系统道)+CS+HST'。相应地，淹死类型顺序边界被变成暴露类型那条。因此，秒顺序和第三顺序的序列有类似的沉积外形的体系结构。有这些时间的变化的伴随物，更低与包含五个第三顺序的序列的1000m的厚度寒武纪被变成不能与加深沉积环境向东南识别第三顺序的序列的压缩继任。根据第三顺序的序列的基本特征，即整齐o在空间和沉积环境的变化的同步性的沉积外形的继任及时，在在不同paleogeographical背景的主要的记载的节的第三顺序的序列的详细部门成为基础建立能在stratigraphic记录表明改变外形的表面的二种类型和diachronism的二种类型的sequence-stratigraphic框架。这个sequence-stratigraphic框架从盆外形的黑页岩形成的压缩继任的库显示出碳酸盐平台的成长进程。从Sinian在拐弯源于快速的违反到寒武纪生态的空间变得开，它形成了为“寒武纪的生物爆炸”设定的paleogeographical的先前的条件。最终，在depositional事件之间的基因关系和生物差异的事件是很复杂的并且在那里仍然是以后需要推进研�

简介：The2008Wenchuanearthquake,amajorintraplateearthquakewithMw7.9,occurredontheslowlydeformingLongmenshanfault.Tobetterunderstandthecausesofthisdevastatingearthquake,weneedknowledgeoftheregionalstressfieldandtheunderlyinggeodynamicprocesses.Here,wedeterminefocalmechanismsolutions(FMSs)ofthe2008Wenchuanearthquakesequence(WES)usingbothP-wavefirst-motionpolaritydataandSH/Pamplituderatio(AR)data.AsP-wavepolaritiesaremorereliableinformation,theyaregivenpriorityoverSH/PAR,thelatterofwhichareusedonlywhentheformerhaslooseconstraintontheFMSs.Wecollectdatafromthreecategories:(1)permanentstationsdeployedbytheChinaEarthquakeAdministration(CEA);(2)theWesternSichuanPassiveSeismicArray(WSPSA)deployedbyInstituteofGeology,CEA;(3)globalstationsfromIncorporatedResearchInstitutionsforSeismology.Finally,129eventswithmagnitudeoverMs4.0inthe2008WESareidentifiedtohavewell-constrainedFMSs.Amongthem,83arewellconstrainedbyP-wavepolaritiesonlyasshownbyCaietal.(EarthqSci24(1):115–125,2011),andtherestofwhicharenewlyconstrainedbyincorporatingSH/PAR.BasedonthespatialdistributionandFMSsoftheWES,wedrawfollowingconclusions:(1)theprinciplecompressionaldirectionsofmostFMSsoftheWESaresubhorizontal,generallyinagreementwiththeconclusiongivenbyCaietal.(2011)butwithafewmodificationsthatthecompressionaldirectionsareWNW–ESEaroundWenchuanandENE–WSWaroundQingchuan,respectively.ThesubhorizontalcompressionaldirectionalongtheLongmenshanfaultfromSWtoNEseemstohavealeftlateralrotation,whichagreeswellwithregionalstressfieldinvertedbyformerresearchers(e.g.,Xuetal.,ActaSeismolSin30(5),1987;ActaGeophysSin32(6),1989;Cuietal.,SeismolGeol27(2):234–242,2005);(2)theFMSsoftheeventsnotonlyreflectedtheregionalstressstateoftheLongmenshanregion,butalsowereobviouslycontrolledbythefaultstosomeextent,whichwaspointedou

简介：Novelpyrimidinenucleoside-3,5-dicyanopyridinehybrids(4)orpyridineattachedacylureas(5)wereselectivelyandefficientlypreparedfromthereactionof2'-deoxyuridin-5-yl-methylenemalononitrile(1),malononitrile(2)andthiophenol(3)orfromanunexpecteduracilring-openingandpyridinering-formingsequenceviathereactionof1and3.Itisthefirsttimesuchasequencehaseverbeenreported.

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简介：Theeffectofpromotercobaltandthesequencesofaddingcobaltandmolybdenumprecursorsontheperformanceofsulfur-resistantmethanationwereinvestigated.AllthesesampleswerepreparedbyimpregnationmethodandcharacterizedbyN2-adsorption,X-raydiffraction(XRD),temperature-programmedreduction(TPR)andlaserRamanspectroscopy(LRS).TheconversionsofCOforMo-Co/Al,Co-Mo/AlandCoMo/Alcatalystswere59.7%,54.3%and53.9%,respectively.Amongthesecatalysts,theMo-Co/AlcatalystpreparedstepwiselybyimpregnatingMoprecursorfirstlyshowedthebestcatalyticperformance.Meanwhile,theconversionsofCOwere48.9%forMo/Alcatalystand10.5%forCo/Alcatalyst.TheadditionofcobaltspeciescouldimprovethecatalyticactivityofMo/Alcatalyst.TheN2-adsorptionresultsshowedthatCo-Mo/Alcatalysthadthesmallestspecificsurfaceareaamongthesecatalysts.CoMoO4speciesinCoMo/AlcatalystweredetectedwithXRD,TPRandLRS.Moreover,crystalMoS2whichwasreportedtobelessactivethanamorphousMoS2wasfoundinbothCo-Mo/AlandCoMo/Alcatalysts.Mo-Co/Alcatalystshowedthebestcatalyticperformanceasithadanappropriatesurfacestructure,i.e.,nocrystalMoS2andverylittleCoMoO4species.

简介：Kr眉p像像素的因素8(KLF8)抄写因素在房间周期前进起一个关键作用，oncogenic转变，对间充质的转变和侵略上皮。然而，它的原子本地化信号(NLS)没被识别。有另外的KLFmonopartiteNLS(mNLS)和C2H2锌手指(ZF)的KLF8份额，哪个被显示了是为一些另外的KLF的NLS。在这份报告，用指导PCR的mutagenesis和immunofluorescent显微镜学，我们显示出mNLSs，任何单个ZF的删除，或变化的那混乱Zn2+有约束力或联系DNA主题没影响KLF8的原子本地化。删除>然而，从C终点的1.5ZF引起了KLF8的细胞质的累积。令人惊讶地，氨基酸(aa)的删除151-200区域几乎从原子核消除了KLF8。有PKC禁止者的S165A，K171E或K171R变化，或处理导致了部分细胞质的累积。Co-immunoprecipitation证明KLF8与importin-交往了，这个相互作用要求了ZF主题。aa1-150或201-261区域的删除独自没改变原子本地化。BrdU加入和cyclinD1倡导者酶试金作为野类型的KLF8证明在原子本地化的KLF8异种有缺陷者不能支持DNA合成或cyclinD1倡导者激活。一起拿，这些结果建议KLF8有二NLS，一包围S165和K171并且另外的是二双人脚踏车ZF，它为KLF8原子本地化和它的细胞的功能的规定是批评的。

简介：Althoughthegreyforecastingmodelhasbeensuccessfullyadoptedinvariousfieldsanddemonstratedpromisingresults,theliteraturesshowitsperformancecouldbefurtherimproved,suchasfortheDGM(1,1)model,basedonaconcavesequence,themodelingerrorwillbelarger.Inthispaper,firstlythedefinitionofsequenceconvexityisgivenout,anditisprovedthattheoutputsequenceofDGM(1,1)modelisaconvexsequence.Next,theresidualchangelawofDGM(1,1)modelbasedontheconcavesequenceisdiscussed,andthenon-equidistanceDGM(1,1)modelisproposed.Finally,byintroducingthesymmetrytransformation,aconcavesequenceistransformedintoaconvexsequence,calledthesymmetricsequenceoftheconcavesequence,andthenconstructthenon-equidistanceDGM(1,1)modelbasedontheconvexsequence.Theexampleresultsshowthatthenovelmethodismoreaccuratethanthedirectmodelingforaconcavesequence.

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简介：AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused many deaths and contributed to a tremendous public health concern worldwide since 2020. Angiotensin-converting enzyme 2 (ACE2) binds to the SARS-CoV-2 virus as a receptor. The challenge of different nonhuman primate (NHP) species by SARS-CoV-2 virus demonstrated different effects on virus replication and disease pathology. This study characterizes differences between host ACE2 sequences of three NHP species: Macaca mulatta, Macaca fascicularis, and Chlorocebus sabaeus. In addition, the binding affinity between the ACE2 ectodomain and the SARS-CoV-2 S receptor-binding domain (RBD) was analyzed. Variation of ACE2 sequence among NHP species and the binding affinity may account for different susceptibility and responses to SARS-CoV-2 infection.