简介：BasedonthenaturalandsocialconditionsaswellashydrogeologicalcharacteristicsoftheRyukyulimestone,amajoraquiferintheRyukyuIslands,aconceptionofundergrounddam,wasproposedintheearly1970sinordertodevelopgroundwaterresourcesintheQuatemaryRyukyulimestoneregionsofJapan.Thepracticeofnearlythirtyyearshasshownthattheundergrounddamisanenvironment-friendlyandeffectivewayfordevelopinggroundwaterintheseregions.``Tofurtherimprovethetechnologyassociatedwithundergrounddam,theauthorsintroducedrelatedanalyticmethods.Theapplicationofthegeographicalinformationsystemtechnologytositeselectionoftheundergrounddamisreportedinthispaper.``

简介：Betulin，oleanolic酸，和betulinic酸是自然地发生的pentacyclictriterpenoids重要药用的价值。这些triterpenoids的可观的数量是可得到的在外部白色吠叫桦树。在这研究，我们使用了帮助超声的抽取(UAE)从桦树提取triterpenoids很快并且与高效率吠叫。用高效液体层析(HPLC)，triterpenoids的三种类型被分开并且检测。我们在提取从的triterpenoids之中检验了差别双对tetraploid白人桦树。然后，我们使用了因素分析外面与包括地优秀的性能屏蔽tetraploid白人桦树。结果显示为抽取的最佳条件作为抽取溶剂包括乙醇的使用，0.1的solid-to-liquid比率?g/10?ml，在100点的超声的力量集合?W，60的温度?

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简介：二细菌的老家(BB)抵抗基因，Xa21和Xa4，从IRBB24被介绍进杂交稻restorer线Mianhui725，它高度产生BB，由使用杂交和分子的帮助标记的选择技术。四根相应恢复系线通过与分子的标记测试R目标基因并且分析父母遗传背景被获得。有特定的紧张ofXanthomonasoryzaepv的四根线和他们的混血儿的接种。oryzae，P1，P6和七个代表汉语拉紧病理型，CⅠ-CⅦ，证明所有四根线和他们的混血儿是到BB的高度抵抗、介绍的宽广抵抗光谱。G46A/R207-2的混血儿显示了好农学的人物和高产潜力，并且R207-2被称为Shuhui207。

简介：ToselecttheimmunogenicshortpeptidemimicsofmalewormoriginofSchistosomajaponicum(Sj)andtoexploretheirprotectioneffectagainstschistosomiasisinmice,therandomphagedisplaypeptidehbraryof12-merwasscreenedwithIgGtosolublemalewormantigenofSj,andthespecificpositiveclonesselectedthroughthreeroundsofscreeningsweredetectedbyDot-ELISA,andtheninjectedsubcutaneouslyintomiceforvaccinationandprotectionassessmentagainstSj.Itwasfoundthat18randomlypickedphagedisplayedclonesallshoweddefiniteantigenicitywithvariousintensities.Thepooledphagesdisplayedclonescouldinduceproductionofspecificantibodiesandcause31.72%ofwormreductionrateand51.54%ofeggreductionrateinmice,revealingasignificantdifference(P<0.001)incomparisonwiththoseofthecontrols.ItconcludesthattheshortpeptidemimicsofmalewormoriginofSjobtainedbyaffinityscreeningphagedisplayptidelibrarycanelicitpartialprotectionagainstthispathogen.

简介：Inthispaper,weproposedawaytorealizeanEr-dopedrandomfiberlaser(RFL)withadisorderedfiberBragggrating(FBG)array,aswellastocontrolthelasingmodeoftheRFLbyheatingspecificlocationsofthedisorderedFBGarray.ThedisorderedFBGarrayperformsasboththegainmediumandrandomdistributedreflectors,whichtogetherwithatunablepointreflectorformtheRFL.Coherentmulti-moderandomlasingisobtainedwithathresholdofbetween7.5and10mWandapowerefficiencybetween23%and27%whenthereflectivityofthepointreflectorchangesfrom4%to50%.Tocontrolthelasingmodeofrandomemission,aspecificpointofthedisorderedFBGarrayisheatedsoastoshiftthewavelengthoftheFBG(s)atthispointawayfromtheotherFBGs.Thus,differentresonancecavitiesareformed,andthelasingmodecanbecontrolledbychangingthelocationoftheheatingpoint.

简介：Theissueofselectionofbandwidthinkernelsmoothingmethodisconsideredwithinthecontextofpartiallylinearmodels.Inthispaper,westudytheasymptoticbehaviorofthebandwidthchoicebasedongeneralizedcross-validation(GCV)approachandprovethatthisbandwidthchoiceisasymptoticallyoptimal.Numericalsimulationarealsoconductedtoinvestigatetheempiricalperformanceofgeneralizedcross-validation.

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简介：Hyper-spectralimagingspectrometerhashighspatialandspectralresolution.Itsradiometriccalibrationneedstheknowledgeofthesourcesusedwithhighspectralresolution.Inordertosatisfytherequirementofsource,anon-orbitradiometriccalibrationmethodisdesignedinthispaper.Thischainisbasedonthespectralinversionaccuracyofthecalibrationlightsource.Wecompilethegeneticalgorithmprogresswhichisusedtooptimizethechanneldesignofthetransferradiometerandconsiderthedegradationofthehalogenlamp,thusrealizingthehighaccuracyinversionofspectralcurveinthewholeworkingtime.Theexperimentalresultsshowtheaveragerootmeansquarederroris0.396%,themaximumrootmeansquarederroris0.448%,andtherelativeerrorsatallwavelengthsarewithin1%inthespectralrangefrom500nmto900nmduring100hoperatingtime.Thedesignlaysafoundationforthehighaccuracycalibrationofimagingspectrometer.

简介：为phosphoenolpyruvatecarboxylase基因(ZmC4Ppc)的未经触动的Zeamays基因全身是6781bp。为这基因的PCR的产品不与差的重覆性是清楚的，结果因为它在米饭和玉米两个都对帮助标记的选择(妈)困难。为为妈选择标记，仅仅在玉米而非在米饭介绍的序列被强风识别，并且用教材首相5.0为教材设计使用了。一双特定的教材称为MRpc(向前：5AAGCAGGGAAGCGAGACG3，逆行：5GATTGCCGCCAGCAGTAG3)被用于转变米饭的选择，并且ZmC4Ppc能高度并且组成地在使用MRpc选择的转变米饭在每个测试发展阶段被表示。因此，MRpc被用于与ZmC4Ppc在米饭和一些restorer线带ZmC4Ppc基因的子孙的妈(例如FPM881)与restorer线Shuhui881源于转变ZmC4Ppc的Kitaakebackcrossed被获得。基因背景上的分析，PEPCase活动，捕捞光合的率，一般联合能力(GCA)和显示出的FPM881的特定的联合能力(SCA)超过95%到达的基因背景的那类似，PEPCase和网络光合的率比那些高控制，并且一些带ZmC4Ppc基因的子孙为谷物的GCA和SCA最好每植物让步，控制比那些每植物，和1000谷物重量圆锥花序数。这建议经由妈和它的稳定的表示的玉米ZmC4Ppc基因的介绍能增加米饭的谷物产量并且将多半为米饭提供一条小径品种的改进。

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简介：TosolvetheproblemsoftheAMR-WB+(ExtendedAdaptiveMulti-Rate-WideBand)semi-open-loopcodingmodeselectionalgorithm,featuresforACELP(AlgebraicCodeExcitedLinearPrediction)andTCX(TransformCodedeXcitation)classificationareinvestigated.11classifyingfeaturesintheAMR-WB+codecareselectedand2novelclassifyingfeatures,i.e.,EFM(EnergyFlatnessMeasurement)andstdEFM(standarddeviationofEFM),areproposed.Consequently,anovelsemi-open-loopmodeselectionalgorithmbasedonEFMandselectedAMR-WB+featuresisproposed.TheresultsofclassifyingtestandlisteningtestshowthattheperformanceofthenovelalgorithmismuchbetterthanthatoftheAMR-WB+semi-open-loopcodingmodeselectionalgorithm.

简介：Centralvillageselectionisthepriorityofsmalltownconstruction.Dependingonthedevelopmentpotentialanalysis,classificationanalysis,andquantitativeandqualitativeanalysis,thisarticlesystematicallyproposesthespecificmethodsofcentralvillageselectionandalsoconstructstheindicatorsystemusedincentralvillageselectioninsmalltownsofwesternunderdevelopedregioninChina.ThearticlealsoputsforwardideasandmethodsforselectionofcentralvillageinthewesternunderdevelopedregionthroughanempiricalresearchonLidianTown,JingningCounty,GansuProvince.Inthefinalpart,suggestionsandrecommendationsonhowtodevelopcentralvillageforLidianTownaremade.

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简介：Publicparksprovidemanybenefitstothecommunityastherepresentativesofgreenarea.Theallocationofpublicplacesplaysanextremelyimportantroleinthedailylivesofinhabitantsespeciallyforrecreationalusethatcouldenhancethequalityoflifeofresidentsinthevicinity.Tounderstandparkusers'behaviorisoneofthemostimportantprerequisitesforassessingtheparticipationinpublicservicefromtheparkusers'pointofview.Thepatternofparkutilizationonlocationandactivityselectionareimportantelementsinbehavioralstudy,whilethepublicparkstopographmayalsoinfluencethetypicaluser'sbehavior.Questionnairesurveyonparkutilizationwasusedtoinvestigatetheinteractionbetweenactivityinvolvementandrecreationallocationwiththeuseoflineardiscriminantanalysis(LDA)model.Thestudyfoundthatpublicparkusers'behaviorisinfluencednotonlybysocialcharacteristicsbutalsobytherecreationalactivitiesandtheirspecificlocationcharacteristics.Wefoundthatabout45percentofparkvisitorsarelocalresidentslivingwithinaradiusof3kmpreferredtraveltoparksneartheirresidentialarea.Thisimpliesthatlocationselectionbehavioriscorrelatedwithtraveldistance,traveltimeandtravelcost.Visitfrequenciesandonsiteexpendituresreflecttherecreationbehaviorfordifferenttypeofactivities.Theoverallinformationcanbeusefullyappliedbydecisionmakerstolaunchappropriatepublicpolicyinconsistencewiththeusefulresultsofthisstudy.

简介：AbstractMany factors have been identified as having the ability to affect the sensitivity of rapid antigen detection (RAD) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to identify the impact of sample processing on the sensitivity of the RAD tests. We explored the effect of different inactivation methods, viral transport media (VTM) solutions, and sample preservation on the sensitivity of four RAD kits based on two SARS-CoV-2 strains. Compared with non-inactivation, heat inactivation significantly impacted the sensitivity of most RAD kits; however, β-propiolactone inactivation only had a minor effect. Some of the VTM solutions (VTM2, MANTACC) had a significant influence on the sensitivity of the RAD kits, especially for low viral-loads samples. The detection value of RAD kits was slightly decreased, while most of them were still in the detection range with the extension of preservation time and the increase of freeze-thaw cycles. Our results showed that selecting the appropriate inactivation methods and VTM solutions is necessary during reagent development, performance evaluation, and clinical application.

简介：Theaccuracyoflaser-inducedbreakdownspectroscopy(LIBS)quantitativemethodisgreatlydependentontheamountofcertifiedstandardsamplesusedfortraining.However,inpracticalapplications,onlylimitedstandardsampleswithlabeledcertifiedconcentrationsareavailable.Anovelsemi-supervisedLIBSquantitativeanalysismethodisproposed,basedonco-trainingregressionmodelwithselectionofeffectiveunlabeledsamples.Themainideaoftheproposedmethodistoobtainbetterregressionperformancebyaddingeffectiveunlabeledsamplesinsemi-supervisedlearning.First,effectiveunlabeledsamplesareselectedaccordingtothetestingsamplesbyEuclideanmetric.Twooriginalregressionmodelsbasedonleastsquaressupportvectormachinewithdifferentparametersaretrainedbythelabeledsamplesseparately,andthentheeffectiveunlabeledsamplespredictedbythetwomodelsareusedtoenlargethetrainingdatasetbasedonlabelingconfidenceestimation.Thefinalpredictionsoftheproposedmethodonthetestingsampleswillbedeterminedbyweightedcombinationsofthepredictionsoftwoupdatedregressionmodels.Chromiumconcentrationanalysisexperimentsof23certifiedstandardhigh-alloysteelsampleswerecarriedout,inwhich5sampleswithlabeledconcentrationsand11unlabeledsampleswereusedtotraintheregressionmodelsandtheremaining7sampleswereusedfortesting.Withthenumbersofeffectiveunlabeledsamplesincreasing,therootmeansquareerroroftheproposedmethodwentdownfrom1.80%to0.84%andtherelativepredictionerrorwasreducedfrom9.15%to4.04%.

简介：AbstractObjective:Optimal reference genes are critical for accurate normalization and reliable interpretation of gene expression quantification data. Recently, several strategies have been utilized for validating reference genes in different human tissues. However, no universal reference genes have been described that accurately summarize transcriptional activity in human spermatozoa.Methods:Using quantitative reverse transcription-polymerase chain reaction (RT-qPCR), we evaluated ten commonly used candidate reference genes between two groups of human cryopreserved donor sperm with different pregnancy rates. We assessed the stability of reference genes using three different algorithms, namely geNorm, NormFinder, and BestKeeper. We then identified the most stable reference genes.Results:Male-enhanced antigen 1 (MEA1) was identified as the most stably expressed reference gene, followed by testis-enhanced gene transcript (TEGT).Conclusions:We comprehensively identified MEA1 and TEGT as the most stably expressed reference genes for the normalization of gene expression data in human spermatozoa.

简介：Orthogonalfrequencydivisionmultiplexing(OFDM)whichhasbeenadoptedinthelong-termevolution(LTE)systemcanimprovethesystemcapacityobviously.However,italsobringsaboutsevereinter-cellinterference(ICI)forcell-edgeusers(CEUs).Totacklethisproblem,multi-userselectionandpowercontrol(MuS-PC)isproposedasanefficientschemeinuplinkcoordinatedmulti-pointmulti-usermulti-inputmulti-output(CoMP-MU-MIMO)transmission/reception.Thispaperjointlyconsidersuser’ssignaltointerferenceplusnoiseratio(SINR)andproportionalfairness(PF)tomaximizethetotalchannelcapacityinmulti-userselectionbyformulatingapenaltyfunction.Tosimplifythepenaltyfunction’scomputation,particleswarmoptimization(PSO)algorithmisintroduced.Inaddition,powercontrolisadoptedtomaximizeoverallenergyefficiency.SimulationresultsdemonstratethattheMuS-PCschemecannotonlyobtaintheoptimaltotalchannelcapacitywhileguaranteeeachuser’squalityofservice(QoS)andPF,butalsolargelyreducecomputationalcomplexityandimproveenergyefficiency.Asaresult,thepoorcommunicationqualityofCEUscanbeenhanced.