简介：Thisstudyaimedtoexploretheroleofmechanicaltensioninhypertrophicscarsandthechangeinnervedensityusinghematoxylin-eosinstainingandS100immunohistochemistry,andtoobservetheexpressionofnervegrowthfactorbywesternblotanalysis.Theresultsdemonstratedthatmechanicaltensioncontributedtotheformationofahyperplasticscarinthebackskinofrats,inconjunctionwithincreasesinbothnervedensityandnervegrowthfactorexpressioninthescartissue.Theseexperimentalfindingsindicatethatthecutaneousnervoussystemplaysaroleinhypertrophicscarformationcausedbymechanicaltension.

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简介：Ciliaryneurotrophicfactoristheonlyknownneurotrophicfactorthatcanpromotedifferentiationofhippocampalneuralprogenitorcellstoglialcellsandneuronsinadultrats.Thisprocessissimilartospontaneousdifferentiation.Therefore,ciliaryneurotrophicfactormaybeinvolvedinspontaneousdifferentiationofneuralstemcells.Toverifythishypothesis,thepresentstudyisolatedneuralprogenitorcellsfromadultmaleratsandculturedtheminvitro.Resultsshowedthatwhenneuralprogenitorcellswereculturedintheabsenceofmitogenfibroblastgrowthfactor-2orepidermalgrowthfactor,theyunderwentspontaneousdifferentiationintoneuronsandglialcells.Westernblotandimmunocytochemicalstainingshowedthatexogenousciliaryneurotrophicfactorstronglyinducedadulthippocampalprogenitorcellstodifferentiateintoneuronsandglialcells.Moreover,passage4adulthippocampalprogenitorcellsexpressedhighlevelsofendogenousciliaryneurotrophicfactor,andaneutralizingantibodyagainstciliaryneurotrophicfactorpreventedthespontaneousneuronalandglialdifferentiationofadulthippocampalprogenitorcells.Theseresultssuggestthatthespontaneousdifferentiationofadulthippocampalprogenitorcellsismediatedpartiallybyendogenousciliaryneurotrophicfactor.

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简介：Objective:Thepredictiveandprognosticroleofprognosticnutritionalindex(PNI)ingastriccancerpatientswithperitonealdisseminationremainsunclear.ThisstudyaimstoexploretheroleofthePNIinpredictingoutcomesofgastriccancerpatientswithperitonealdissemination.Methods:Atotalof660patientsdiagnosedwithgastricadenocarcinomawithperitonealmetastasisbetweenJanuary2000andApril2014atSunYat-senUniversityCancerCenterandtheSixthAffiliatedHospitalofSunYatsenUniversitywereretrospectivelyanalyzed.Theclinicopathologiccharacteristicsandclinicaloutcomesofpatientswithperitonealdisseminationwereanalyzed.Results:ComparedwithPNI-highgroup,PNI-lowgroupwascorrelatedwithadvancedage(P=0.036),worseperformancestatus(P<0.001),higherfrequencyofascites(P<0.001)andhigherfrequencyofmultisitedistantmetastasis(P<0.001).Kaplan-MeiersurvivalcurvesshowedthatPNI-highgrouphadasignificantlylongermedianoverallsurvivalthanPNI-lowgroup(13.13vs.9.03months,P<0.001).MultivariatesurvivalanalysisrevealedthatBorrmanntypeIV(P=0.014),presenceofascites(P=0.017)andlowerPNI(P=0.041)wereindependentpoorprognosticfactors,andpalliativesurgery(P<0.001)andfirst-linechemotherapy(P<0.001)weregoodprognosticfactors.Forpatientsreceivingpalliativesurgery,thepostoperativemorbidityratesinthePNI-lowgroupandPNIhighgroupwere9.1%and9.9%,respectively(P=0.797).ThepostoperativemortalityratewasnotsignificantlydifferentbetweenPNI-lowandPNI-highgroups(2.3%vs.0.9%,P=0.362).Conclusions:PNIisausefulandpracticaltoolforevaluatingthenutritionalstatusofgastriccancerpatientswithperitonealdissemination,andisanindependentprognosticfactorforthesepatients.

简介：Asecond-ordermixingdifferenceschemewithalimitingfactorisdeducedwiththereconstructiongradientmethodandappliedtodiscretizingtheNavier-Stokesequationinanunstructuredgrid.Thetransformofnonorthogonaldiffusionitemsgeneratedbytheschemeindiscreteequationsisprovided.TheDelaunaytriangulationmethodisimprovedtogeneratetheunstructuredgrid.ThecomputingprogrambasedontheSIMPLEalgorithminanunstructuredgridiscompiledandusedtosolvethediscreteequationsoftwotypesofincompressibleviscousflow.Thenumericalsimulationresultsofthelaminarflowdrivenbylidincavityandflowbehindacylinderarecomparedwiththetheoreticalsolutionandexperimentaldatarespectively.Intheformercase,agoodagreementisachievedinthemainvelocityanddragcoefficientcurve.Inthelattercase,thenumericalstructureanddevelopmentofvortexunderseveralReynoldsnumbersmatchwellwiththatoftheexperiment.Itisindicatedthatthefactordifferenceschemeisofhigheraccuracy,andfeasibletobeappliedtoNavier-Stokesequation.

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简介：Transcriptionfactor(TF)bindingtoitsDNAtargetsiteplaysanessentialroleingeneregulation.Thelocation,orientationandspacingoftranscriptionfactorbindingsites(TFBSs)alsoaffectregulatoryfunctionoftheTF.However,hownucleosomalcontextofTFBSsinfluencesTFbindingandsubsequentgeneregulationremainstobeelucidated.Usinggenome-widenucleosomepositioningandTFbindingdatainbuddingyeast,wefoundthatbindingaffinitiesofTFstoDNAtendtodecreasewithincreasingnucleosomeoccupancyoftheassociatedbindingsites.WefurtherdemonstratedthatnucleosomalcontextofbindingsitesiscorrelatedwithgeneregulationofthecorrespondingTF.Nucleosome-depletedTFBSsarelinkedtohighgeneactivityandlowexpressionnoise,whereasnucleosome-coveredTFBSsareassociatedwithlowgeneactivityandhighexpressionnoise.Moreover,nucleosome-coveredTFBSstendtodisruptcoexpressionofthecorrespondingTFtargetgenes.WeconcludethatnucleosomalcontextofbindingsitesinfluencesTFbindingaffinity,subsequentlyaffectingtheregulationofTFsontheirtargetgenes.ThisemphasizestheneedtoincludenucleosomalcontextofTFBSsinmodelinggeneregulation.

简介：Therearemanyfactorsaffectingtheinstabilityofthesubmarinehydrate-bearingslope(SHBS),andtheinteractionwithhydrateisverycomplicated.Inthispaper,themechanicalmechanismofthestaticliquefactionandinstabilityofsubmarineslopecausedbythedissociationofnaturalgashydrate(NGH)resultingintherapidincreaseofporepressureofgashydrate-bearingsediments(GHBS)andthedecreaseofeffectivestressareanalyzedbasedonthetimeseriesandtypeofSHBS.Then,takingthetypicalsubmarineslopeinthenorthernSouthChinaSeaasanexample,fourimportantfactorsaffectingthestabilityofSHBSareselected,suchasthedegreeofhydratedissociation,thedepthofhydrateburial,thethicknessofhydrate,andthedepthofseawater.Accordingtotheprincipleoforthogonalmethod,25orthogonaltestschemeswith4factorsand5levelsaredesignedandthesafetyfactorsofsubmarineslopestabilityofeachschemearecalculatedbyusingthestrengthreductionfiniteelementmethod.Bymeansoftheorthogonaldesignrangeanalysisandthevarianceanalysis,sensitivityofinfluentialfactorsonstabilityofSHBSareobtained.Theresultsshowthatthedegreeofhydratedissociationisthemostsensitive,followedbyhydrateburialdepth,thethicknessofhydrateandthedepthofseawater.Finally,theconceptofgashydratecriticalburialdepthisputforwardaccordingtotheinfluencelawofgashydrateburialdepth,andthenumericalsimulationforspecificsubmarineslopeiscarriedout,whichindicatestheexistenceofcriticalburialdepth.

简介：客观：在牛的心包的非细胞组成的新鲜标本在endothelial细胞(Ecs)的粘附和增长上调查脉管的endothelial生长因素(VEGF)和它的效果的绑定和版本，它被heparinization.Methods修改:牛的心包的Cross-linked非细胞组成的新鲜标本是由三个方法的heparinized:(1)heparinizedN-(3-dimethylaminopropyl)-N'-ethylcarbodiimidehydrochloride(EDC)对待非细胞组成的织物样品；(2)(ethyleneimine)(PEI)heparinizedpoly对待

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简介：Formulationintermsofhypersingularintegralequationsfortheinteractionbetweenstraightandcurvedcracksinplaneelasticityisobtainedusingthecomplexvariablefunctionsmethod.Thecurvedlengthcoordinatemethodandasuitablenumericalschemeareusedtosolvesuchintegralsnumericallyfortheunknownfunction,whicharelaterusedtofindthestressintensityfactor,SIF.

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简介：Topreparemonoclonalantibodyspecifictoepidermalgrowthfactorreceptor(EGFR)intracellulardomain,itsgenewasamplifiedfromtotalRNAofA431cellbyRT-PCR.ThenthegenewasclonedintoprokaryoticvectorpET30a(+).TherecombinantplasmidwastransformedintoE.ColiBL21(DE3)strainforproteinexpression.RecombinantproteinwasinducedwithIPTGandpurifiedusingNi2+-NTAagarose.Thentheanti-EGFRmonoclonalantibody(nAb)waspreparedwithclassicalhybridomatechnique.Positivecloneswereselectedusingindirectenzyme-linkedinmunoabsorbentassay(ELISA).Totally4hybridomacloneswereobtainedandthesemAbswereIgG1(3clones)andIgG2a(1clone),respectively.Theirlightchainswereallkappachains.WesternblottinganalysisandconfocalimmunofluorescenceassaysdemonstratedthatmAbscouldspecificallyrecognizeEGFRexpressingonA431carcinomacellline.ThemAbswillbeusefulinthestudyofEGFR-mediatedsignaltransduction.

简介：Toexplorethemolecularmechanismoftheprotectiveeffectofnervegrowthfactor(NGF)oninjuredspinalcord.Methods:TheposteriorT8(the8ththoracicsegment)spinalcordsof60Wistarratswereinjuredbyimpactscausedbyobjects(weighing10g)fallingfromaheightof2.5cmwithAllensway.Solutionwithnervegrowthfactors(NGF)wasgivento30rats(theNGFgroup)throughamicrotubuleinsertedintothesubarachnoidcavityimmediately,andat2,4,8,12and24hoursafterspinalcordinjury(SCI)respectively.Normalsaline(NS)withsamevolumewasgiventotheother30rats(theNSgroup)withthesamemethod.And5normalratsweretakenasthenormalcontrols.Theexpressionofbcl-2andbaxproteinsinspinalcordwasdetectedwithimmunohistochemistry.Theapoptoticneuronsinspinalcordweremeasuredwithterminaldeoxynucleotidyltransferase-mediateddUTP-biotinnickend-labelingofDNAfragments(TUNEL)staining.Results:Thepositiveexpressionofbcl-2proteinwasstronginthenormalcontrols,butdecreasedintheNSgroup,andincreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Thepositiveexpressionofbaxproteinwasalsostronginthenormalcontrols,butincreasedintheNSgroup,anddecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).ApoptoticneuronswerefoundintheNSgroup,andtheydecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Conclusions:NGFcanprotecttheinjurednervetissuesthroughstimulatingtheexpressionofbcl-2protein,inhibitingtheexpressionofbaxproteinandinhibitingtheneuronalapoptosisafterSCI.

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简介：Thedynamicstressintensityfactorhistoryforahalfplanecrackinanotherwiseunboundedelasticbody,withthecrackfacessubjectedtoatractiondistributionconsistingoftwopairsofcombinedmodepointloadsthatmoveinadirectionperpendiculartothecrackedgeisconsidered.Theanalyticexpressionforthecombinedmodestressintensityfactorsasafunctionoftimeforanypointalongthecrackedgeisobtained.ThemethodofsolutionisbasedontheapplicationofintegraltransformtogetherwiththeWiener-HopftechniqueandtheCagniard-deHoopmethod.Somefeaturesofthesolutionarediscussedandgraphicalresultsforvariouspointloadspeedsarepresented.

简介：Thestressintensityfactor(SIF)isacriticalparameterassociatedwiththefracturebehaviourofmaterials.Inthispaper,weselectthedisplacementfunctionaroundacracktipastheshapefunctionofthedigitalimagecorrelation(DIC),whichmakesitpossibletodirectlycalculatetheSIFbythecorrelationscheme.Moreover,weuseanon-rectangularsubset,whichcanreducetheinfluenceofplasticdeformationandcrackwidthontheDICmeasurementaccuracy.WemeasuredtheSIFofamodeIcrackinasuper-hardaluminiumalloyspecimentoverifytheperformanceoftheproposedmethod.OurexperimentalresultsshowthataDICwithaspecificshapefunctioncanbeusedtoaccuratelyandefficientlycalculatetheSIF.Furthermore,wealsopresentapracticalapplicationofourproposedmethodfordeterminingtheSIF,crackpropagationangleandcracktipdisplacement.

简介：Gastrodin,anactivecomponentoftallgastrodiatuber,iswidelyusedinthetreatmentofdizziness,paralysis,epilepsy,strokeanddementia,andexhibitsaneuroprotectiveeffect.AratmodelofspinalcordinjurywasestablishedusingAllen’smethod,andgastrodinwasadministeredviathesubarachnoidcavityandbyintraperitonealinjectionfor7days.Resultsshowthatgastrodinpromotedthesecretionofbrain-derivedneurotrophicfactorinratswithspinalcordinjury.Aftergastrodintreatment,themaximumangleoftheinclinedplanetest,andtheBasso,BeattieandBresnahanscoresincreased.Moreover,gastrodinimprovedneuraltissuerecoveryintheinjuredspinalcord.Theseresultsdemonstratethatgastrodinpromotesthesecretionofbrain-derivedneurotrophicfactor,contributestotherecoveryofneurologicalfunction,andprotectsneuralcellsagainstinjury.