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简介：Thisletterstudiesandanalyzestheworkingfeaturesofmaincircuitoftri-levelboostPowerFactorCorrect(PFC)converterandtheadvantagesoftri-levelswitchconverterinaspectsofbearinghigh-voltageofpowercomponents,overallsystemlossandmagneticcomponentselectionbaseduponthesingle-levelboostPFCswitchconverter.Besides,relyingontheapplicationofmi-croprocessorinpowerconvertertechnologyandDSP(DigitalSignalProcessing)chip'sstrongcal-culatingcapacity,theletterpresentstheadoptionofmodifiedschemeoftri-levelboostPFCconverterunderthecontrolofpredictivecontrolalgorithm.Moreover,theoperatingprincipleandcontrolmethodarespecified,theresultsofcircuittestandanalysisareprovidedandtheadvantagesofpre-dictivecontroltechnology-basedmulti-levelboostPFCconverterisverified.

简介：AbstractObjective:Platelet-rich plasma (PRP) releases growth factors upon activation, which in turn accelerates healing and regeneration of the target tissue. However, PRP composition may vary according to the patient’s demographics, and wider applications of PRP warrant product standardization. The current study aimed to examine variables influencing the platelet-derived growth factor BB (PDGF-BB) concentration in PRP.Methods:This observational study was conducted in the Department of Pathology and Dentistry at Swami Rama Himalayan University, a tertiary care hospital in northern India from December 2016 to November 2017. PRP was prepared from 40 mL of whole blood from 35 individuals (22 women, 13 men). Platelet counts, platelet indices (platelet distribution width, mean platelet volume) and PDGF-BB levels were measured, and platelet yield, platelet dose, and growth factor dose in PRP were also calculated. All parameters were analyzed using Pearson’s correlation coefficient. The association between PDGF-BB and PRP platelet count was evaluated using logistic regression. This study was approved by the Ethics Committee of Swami Rama Himalayan University (SRHU/HIMS/ETHICS/2016/103) on September 7, 2016.Results:The mean platelet count, PDGF-BB concentration, platelet yield, platelet dose, and growth factor dose in PRP were 1317×109/L, 30±9.89ng/mL, 71.62±28.34%, 6.5±3.5×109, and 159.62±52.39ng/mL, respectively. Linear regression analysis indicated that PRP platelet counts were a good predictor for PGDF-BB (P<0.05; adjusted R2=0.96. PRP platelet count was significantly positively correlated with PDGF-BB concentration (r=0.74, P<0.001), platelet yield (r=0.80, P<0.001), platelet dose (r=1, P<0.001), and growth factor dose (r=0.74, P<0.001).Conclusions:PRP has wide clinical applications associated with its healing and regenerative properties, and both the quality and quantity of PRP thus need to be standardized as per the requirements. Evaluating variables affecting PRP will thus aid pathologists and clinical practitioners.

简介：人的造血作用用控制干细胞区别的技术被评估，二维的胶化基于电气泳动的proteomics，和功能的基因组学。我们提供神经胶质成熟因素鲸鱼群妈(GMFG)是cytokine应答的蛋白质在的第一份报告导致erythropoietin并且刺激导致因素的造血的系开发的granulocyte殖民地。从全球功能的基因组学分析的结果显示GMFG拥有几个另外的特征：造血的织物特定的基因表示，与高分数的造血作用特定的抄写因素集中的一个倡导者，和有一个原始血/免疫者系统的可能的分子的coevolution。根据我们的调查结果，我们假设那GMFG是可以调停的造血特定的蛋白质人的造血的干细胞的pluripotentiality和系承诺。

简介：AIMTo学习discoidin象我一样domaincontaining蛋白质3的效果(EDIL3)在人的透镜的增长和上皮间充质的转变(EMT)上的弄空上皮的房间(LEC).METHODSRNA干扰被用来在vitro在人的LEC禁止EDIL3的表示。房间的形态学用一台转换显微镜被观察。房间增长用EdU工具包被估计。房间移植用Transwell房间被调查，LEC的EMT用共焦的显微镜并且西方的弄污被估计。转变生长因素(TGF)小径用数据显示出的西方的blotting.RESULTSThe被调查那个silencingEDIL3表达式改变了LEC形态学并且压制了LEC增长(P<0.05)并且移植(P<0.01)。而且，西方的弄污的结果证明那EDIL3弄空减少了光滑的肌肉肌动朊的表示(-SMA)(P<0.001)并且vimentin(P<0.01)，当增加时E-cadherin的表示(P<0.001)。EDIL3弄空能压制Smad2的phosphorylation(P<0.01)并且Smad3(P<0.01)并且exracellular信号的激活调整了kinase(英皇家空军之阶级最低之兵)(P<0.05).CONCLUSIONThe调查结果显示EDIL3可能经由TGF小径在LEC参予增长和EMT并且可以是为以后的囊opacification的处理的一个潜在的治疗学的目标。

简介：AIMTo在cells.METHODSARPE-19细胞是的网膜的颜料上皮(RPE)上调查高葡萄糖层次和反脉管的endothelial生长因素(VEGF)代理人(bevacizumab，ranibizumab和aflibercept)的效果在不同葡萄糖层次(5.5mmol/L，25mmol/L，和75mmol/L)有教养。房间生存能力被MTT试金与D葡萄糖在处理以后在3d评估。房间迁居能力被创伤愈合试金在3d测量。一个房间死亡察觉工具包被用来在3点估计apoptosis并且14d。房间增长被EdU试金在3d估计。文化媒介在临床上相关的集中与anti-VEGF代理人被对待。实验然后在一个不同葡萄糖level.RESULTSThe生存能力被重复，ARPE-19房间的移植显著地作为与5.5mmol/L葡萄糖相比面对75mmol/L被减少。TUNEL积极的房间的百分比显著地被增加，proliferative潜力与5.5mmol/L葡萄糖相比与75mmol/L被减少。在在25mmol/L和5.5mmol/L葡萄糖之间的结果没有重要差别。面对75mmol/L葡萄糖，与anti-VEGF对待的组显示出减少的房间生存能力和增长并且增加了apoptosis。然而，anti-VEGFgroups.CONCLUSIONHigh葡萄糖水平减少之间没有重要差别RPE房间的生存能力，创伤愈合能力，和增长，当增加apoptosis时。而且，anti-VEGF代理人在高葡萄糖的条件下面防碍RPE房间的生理的功能，在房间生存能力和增长由减少伴随了。

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简介：ToexploretherelationshipbetweensubstanceP(SP)releasedfromperipheralnerveendingsandtheexpressionofepidermalgrowthfactor(EGF)andepidermalgrowthfactorreceptor(EGFR)duringwoundhealing.Methods:FiftyWistarratswererandomlydividedinto2groups,injurygroupandcapsaicingroup.Intheinjurygroup,afull-thicknessskinwoundonthebackoftheratwastaken.Thewoundedgeandgranulationtissuesweretakenonthe1st,3rd,6th,9th,12thdaysafterinjury,respectively.Inthecapsaicingroup,capsaicinwasinjectedsubcutaneouslyonthebackoftheratstodestroythesensorynervetopreventthesecretionofSP,thenawoundandsamplewasmadeinthesameway.ImmunohistochemistryandinsituhybridizationwereemployedtodetecttheexpressionofSP,EGF/EGFR,andEGFmRNA/EGFRmRNAinthegranulationtissues.Results:Intheinjurygroup,immunohistochemicalstainofSPandEGF/EGFRwaslocatedonthehairfolliclesandsebaceousglandsatthe1stday.AndthestainofSPwasobviousatthe3rddayinthegranulationtissues,thendecreasedgradually.EGF/EGFRwasatlowlevelatthe3rdday,thenincreasedgraduallyandreachedthepeakatthe9thday,thendeclined.Inthecapsaicingroup,theimmunohistochemicalstainofSPandEGF/EGFRwasfaintandwithoutobviouschangeduringthewoundhealingprocess.ThetendencyoftheEGFmRNA/EGFRmRNAexpressionwassimilartothatofEGF/EGFR.Conclusions:Duringwoundhealing,SPmaypromotethehealingprocessbyaffectingtheexpressionofEGF/EGFRinthegranuationtissues.

简介：TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.

简介：有细密纹理的岩石(FGR)是重要来源岩石和目前是前景热点的页岩烃的水库。广泛地分布式的有细密纹理的沉积(FGS)上面在Dongying消沉的Shahejie形成的第四个成员作为一个例子被带在这份报纸学习时空进化和FGS的控制因素。基于井核心的分析，薄节，无机、器官的地球化学指示物，FGR被划分成岩相的7种类型。通过point-line-plane的学习，这研究证明FGS有rhythum，差异和继任的特征。第一个阶段被粘土似的FGS(巨大的claystone)描绘。第二个阶段被碳酸盐FGS描绘(low-TOC把石灰石压成薄片)并且含白云石的FGS(含白云石淤泥的页岩)由违反形成了。第三个阶段被器官富人的碳酸盐FGS描绘(middle/high-TOC把石灰石压成薄片)在分布式骑车。第四个阶段被FGS描绘混合碳酸盐和siliciclastic沉积(石灰质淤泥的页岩)。FGS的许多时空进化被多重因素包括tectonism，气候和湖条件控制。

简介：Parkinson’sdisease(PD)ischaracterizedbytheprogressivelossofmidbraindopaminergic(mDA)neuronsandasubsequentdecreaseinstriataldopaminelevels,whichcausethetypicalclinicalmotorsymptomssuchasmusclerigidity,bradykinesiaandtremor.Althoughasubsetof

简介：TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.

简介：AbstractBackground:B-cell activating factor (BAFF) is vital for B cell survival. Serum BAFF levels are elevated in thrombotic antiphospholipid syndrome, but little is known about levels in patients with positive antiphospholipid antibodies (aPLs) and previous adverse pregnancy outcomes (APOs). We aimed to analyze serum BAFF concentrations of these patients in early pregnancy along with different pregnancy outcomes.Methods:Thirty-six pregnant patients positive for aPLs and previous APOs (patient group), 25 healthy pregnant females (HP group) and 35 healthy non-pregnant females (HNP group) from the Peking University Third Hospital, between October 2018 and March 2019, were enrolled in this study. Serum of HNP and serum of patients as well as HP in the first gestational trimester were collected. Enzyme-linked immunosorbent assay kits were used to measure serum BAFF and interferon-alpha (IFN-α) concentrations. Cytometric bead array analysis was used to measure serum concentrations of cytokines. The patient group was further divided into APOs and non-APOs (NAPOs) group, fetal loss and live birth group according to pregnancy outcomes. The Mann-Whitney U-test was used to assess significance between and within groups. Spearman rank-order was used to evaluate correlation coefficients between BAFF and related cytokines.Results:The serum BAFF level in HP group was significantly lower than HNP group (245.24 [218.80, 265.90] vs. 326.94 [267.31, 414.80] pg/mL, Z = -3.966, P < 0.001). The BAFF level was obviously elevated in patient group compared to that in HP group (307.77 [219.86, 415.65] vs. 245.24 [218.80, 265.90] pg/mL, Z = -2.464, P = 0.013). BAFF levels in APOs group tended to be higher than that in NAPOs group (416.52 [307.07, 511.12] vs. 259.37 [203.59, 375.81] pg/mL, Z = -2.718, P = 0.006). Compared to HP group, concentrations of IFN-α, interleukin (IL-6) and tumor necrosis factor were higher in patient group (33.37 [18.85, 48.12] vs. 13.10 [6.85, 25.47] pg/mL, Z = -2.023, P = 0.043; 39.16 [4.41, 195.87] vs. 3.37 [2.92, 3.90] pg/mL, Z = -3.650, P < 0.001; 8.23 [2.27, 64.46] vs. 1.53 [1.25, 2.31] pg/mL, Z = -3.604, P < 0.001, respectively). Serum BAFF levels had a positive correlation with the concentrations of both IL-6 and IL-10 (IL-6: r = 0.525, P = 0.002; IL-10: r = 0.438, P = 0.012).Conclusions:Serum BAFF levels are increased in patients with positive aPLs and previous APOs as compared to healthy pregnant females and tend to be higher in individuals with current APOs. The BAFF levels have a positive correlation with serum IL-6 and IL-10.

简介：AbstractNearly 70% of breast cancer (BC) is hormone-receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative, and endocrine therapy is the mainstay of treatment for this subtype. However, intrinsic or acquired endocrine resistance can occur during the endocrine treatment. Based on insights of endocrine resistance mechanisms, a number of targeted therapies have been and continue to be developed. With regard to HR-positive, HER2-negative advanced BC, aromatase inhibitor (AI) is superior to tamoxifen, and fulvestrant is a better option for patients previously exposed to endocrine therapy. Targeted drugs, such as cyclindependent kinases (CDK) 4/6 inhibitors, mammalian target of rapamycin (mTOR) inhibitors, phosphoinositide-3-kinase (PI3K) inhibitors, and histone deacetylase (HDAC) inhibitors, play a significant role in the present and show a promising future. With the application of CDK4/6 inhibitors becoming common, mechanisms of acquired resistance to them should also be taken into consideration.

简介：AbstractBackground:Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. It has been demonstrated that microRNA-145 (miR-145) is correlated with the progression of various cancers by regulating the expression of multiple target genes, especially a number of genes that regulate angiogenesis and proliferation. However, the underlying mechanisms of miR-145 in tumor angiogenesis of UM are still not well illustrated. Thus, we aimed to explore the potential target genes or pathways regulated by miR-145 in UM and the effect of miR-145 on invasion and angiogenesis.Methods:Totally, 24 choroid samples were collected in our study, including 12 UM samples and 12 normal uveal tissues. The expression of neuroblastoma RAS viral oncogene homolog (N-RAS), phosphorylated protein kinase B (p-AKT), and vascular endothelial growth factor (VEGF) in UM tissues and normal uveal tissues was analyzed using Western blotting analysis. Lentivirus expression system was used to construct MUM-2B and OCM-1 cell lines with stable overexpression of miR-145. Transwell and endothelial cell tube formation assay were used to measure the effects of miR-145 on the invasion and angiogenesis of UM in vitro. The downstream target genes of miR-145 were predicted by bioinformatics and confirmed using a luciferase assay. BALB/c nude mice models were established to investigate the mechanisms of miR-145 on tumor growth and angiogenesis in vivo. Group data comparisons were performed using analysis of Student’s t test. A two-tailed P < 0.05 was considered as statistically significant.Results:The results of Western blotting analysis indicated that the expressions of N-RAS (1.10 ± 0.35 vs. 0.41 ± 0.36, t = 3.997, P = 0.012), p-AKT (1.16 ± 0.22 vs. 0.57 ± 0.03, t = 7.05, P = 0.001), and VEGF (0.97 ± 0.32 vs. 0.45 ± 0.21, t = 3.314, P = 0.008) in UM tumor tissues were significantly higher than those in normal uveal tissue. Luciferase assay demonstrated N-RAS and VEGF as downstream targets of miR-145. Moreover, tube formation assay revealed that miR-145-transfected human microvascular endothelial cell line formed shorter tube length (36.10 ± 1.51 mm vs. 42.91 ± 0.94 mm, t = 6.603, P = 0.003) and less branch points (350.00 ± 19.97 vs. 406.67 ± 17.62, t = 3.685, P = 0.021) as compared with controls. In addition, the numbers of invaded MUM-2B and OCM-1 cells with miR-145 overexpression were significantly lower than the controls (35.7 ± 3.3 vs. 279.1 ± 4.9, t = 273.75, P < 0.001 and 69.5 ± 4.4 vs. 95.6 ± 4.7, t = 21.27, P < 0.001, respectively). In vivo, xenografts expressing miR-145 had smaller sizes (miR-145 vs. miR-scr, 717.41 ± 502.62 mm3vs. 1694.80 ± 904.33 mm3, t = 2.314, P = 0.045) and lower weights (miR-145 vs. miR-scr, 0.74 ± 0.46 g vs. 1.65 ± 0.85 g, t = 2.295, P = 0.045).Conclusion:Our results indicated that miR-145 is an important tumor suppressor and the inhibitory strategies against N-RAS/VEGF signaling pathway might be potential therapeutic applications for UM in the future.

简介：在CD4系抄写因素ThPok和CD8系抄写因素之间的相互影响，老牛相关的抄写因素3(Runx3)，在T房间，开发广泛地被记录了。然而，很少在不变的自然漂亮T(iNKT)房间开发对这些抄写因素的角色被知道。限制CD1d的iNKT房间承诺CD4+CD8−和CD4−CD8−sublineages，它对抗原刺激作出回应与快速并且T助手(Th)的有势力版本1并且Th2cytokines。然而，以前的报告在ThPok缺乏的老鼠表明了CD8+NKT房间的一张新人口。在当前的学习，我们寻求了决定Runx3是否当ThPok不在时涉及CD8的重新表示和iNKT房间的功能。我们使用了缺乏Runx3，ThPok或两个的老鼠并且证实Runx3为在ThPok猛烈老鼠的CD8+iNKT房间的外观部分负责。另外，Runx3参予了有免疫力的反应在α的一个模型由iNKT房间调停了；-galactosylceramide-induced尖锐肝炎。这些结果显示Runx3为在ThPok缺乏的iNKT房间观察的phenotypic和功能的变化是关键的。

简介：在monocot米饭种类OryzasativaL.，最惹人注目的词法过程之一在繁殖开发期间是有上面的internodes(合众国际社)的顺序的延伸的圆锥花序开发的同时发生。阐明内在的分子的机制，我们克隆米饭基因颈叶1(NL1)，什么时候变异，它在flowering时间，有簇叶丛生的苞的更小的圆锥花序和反常合众国际社延伸模式导致延期。NL1基因与一个单个锌手指领域，和它的抄本编码一个GATA类型抄写因素在苞primordia主要被检测，它通常在野类型的植物堕落。在转基因的植物的NL1的Overexpression经常产生严重生长延迟，不太植物的phytomers和更小的叶子，建议NL1起在器官区别的一个重要作用。PLASTOCHRON1(PLA1)的新奇变异的等位基因，知道在调整叶开始起一个关键作用的基因，在这研究被识别。基因分析表明了在nl1和pla1之间的一个相互作用，与PLA1在上游的代理的NL1。表示水平和PLA1的空间模式被发现在nl1被改变变异。而且，flowering，Hd3a和OsMADS1的二个管理者的表示，也在nl1异种被影响。根据这些调查结果，我们建议NL1是通过在米饭在繁殖开发期间调整PLA1和另外的规章的基因的表示调制并且协调organogenesis的一个内在的因素。

简介：BACKGROUND:Studieshavedemonstratedthatbrain-derivedneurotrophicfactor(BDNF)hasadualeffectonepilepsy.However,therelationshipbetweenepilepsy-inducedbraininjuryandBDNFremainspoorlyunderstood.OBJECTIVE:Accordingtoultrastructuralandmolecularparameters,todetectthedegreeofneuronalinjuryandBDNFexpressionchangesatdifferentbrainregionsanddifferentkindlingtimestodeterminetheeffectsofBDNFonepilepsy-inducedbraininjury.DESIGN,TIMEANDSETTING:Arandomized,controlled,animalexperimentbasedonneuropathologyandmolecularbiologywasperformedattheDepartmentofPhysiologyandDepartmentofPathology,BasicMedicalCollegeofJilinUniversityin2003.MATERIALS:UltraSensitiveSPkitforimmunohistochemistry(FuzhouMaximBiotechnology,China),BDNFantibody(concentratedtype,WuhanBosterBiologicalTechnology,China),JEM-1000SXtransmissionelectronmicroscopy(JEOL,Japan),andBH-2lightmicroscope(Olympus,Japan)wereusedinthepresentstudy.METHODS:Wistarratswererandomlyassignedtocontrol(n=6),sham-surgery(n=6),andmodel(n=60)groups.Thecontrolgroupratswerenottreated;anelectrodewasembeddedintotheamygdalainratsfromthesham-surgeryandmodelgroups;anamygdalakindlingepilepsymodelwasestablishedinthemodelgroup.MAINOUTCOMEMEASURES:Pathologicalchangesinthetemporallobeandhippocampuswereobservedbylightandelectronmicroscopyat1,3,7,14,and21daysfollowingkindling,andBDNFexpressioninthevariousbrainregionswasdeterminedbyimmunohistochemistry.RESULTS:Inthemodelgroup,temporallobecorticalandhippocampalneuronswereswollenandthenucleiwerelaterallydeviated.Therewerealsosomeapoptoticneurons3daysafterkindling.Thenucleolidisappearedandthenucleiappearedbrokenorlysed,aswellasslightmicrogliahyperplasia,at7days.Electronmicroscopicobservationdisplayedchromatinaggregationinthenucleiandslightmitochondrionswelling3daysafterkindling.Injurychangeswereaggravatedat7days,characteriz

简介：ThestimulatedBrillouinscattering(SBS)thresholdenhancementfactorinapurewhitenoiselinewidthbroadeningYb-dopedfiberamplifier(YDFA)withashortlargemodeareafiberistheoreticallyandexperimentallystudied.Wedemonstratea1064.08nm,11.6GHzlinewidth,1.5kWoutputpowerYDFAwithanSBSthresholdenhancementof~57(26WSBSthresholdwithsinglefrequencyseed).Theoutputbeamisnear-diffractionlimitedwithabeamqualityfactorofM~2=1.15andaslopeefficiencyofupto87%.NoSBSorstimulatedRamanscatteringeffectsareobservedinthewholepowerrange.Furtherpowerscalingislimitedbytheavailablepumppowerinoursystem.

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