简介：

简介：Jimwantstoborrowabookfromanewlibrary(图书馆）.HecomestothelibrarywithJack.Theycan'tseeanyassistants(图书管理员）there,butonlysomerobots（机器人）standingthere.ThenJimsaystotherobot,""Hey,givemethebook.""Buttherobotdoesn'twork.

简介：

简介：Inthispaper,wepresentthecurrentstatusoftheprojectfordevelopingthenumericallibrayrinJAVA.Wehavepresentedhowobject-orientedtechniquesimproveusageandalsodevelopmentofnumericallibrariescomparedwiththeconventionalwayatpreviousconference,weneedmanyfunctionsfordataanalysiswhichisnotprovidedwithinJAVAlanguage,forexample,goodrandomnumbergenerators.specialfunctionsandsoon.Ourdevelopmentstrategyisfocusedoneasinessofimplementationandaddingnewfeaturesbyusersthemselvesnotonlybydevelopers.InHPCfiled,thereareotherfocuseffortstodevelopnumericallibrariesinJAVA,However,theirfocusisontheperformanceofexecution.noteasinessofextension.Followingthestrategy,wehavedegignedandimplementedmoreclassesforrandomnumbergeneratorsandsoon.

简介：我们常说，读书就是同作者对话；既然这样，能不能通过直接与真人交谈，获得需要的知识呢？这正是HumanLibrary的宗旨。

简介：ＤＧＰＳＬ：ＡＤＩＳＴＲＩＢＵＴＥＤＧＲＡＰＨＩＣＳＬＩＢＲＡＲＹＳｈｉＪｉａｏｙｉｎｇ；ＰａｎＺｈｉｇｅｎｇ；ＺｈｅｎｇＷｅｎｔｉｎｇＤＧＰＳＬ：ＡＤＩＳＴＲＩＢＵＴＥＤＧＲＡＰＨＩＣＳＬＩＢＲＡＲＹ￥ＳｈｉＪｉａｏｙｉｎｇ；ＰａｎＺｈｉｇｅｎｇ；...

简介：

简介：

简介：

简介：微软Pattems＆Practices团队的成员GrigoriMelnik在他的博客（blogs．msdn．com／agile）上宣布了最新版本EnterpriseLibrary5．0的正式发布。虽然EnterpriseLibrary并非一个正式产品，但由于它的半“官方”性质，EnterpriseLibrary一直受到．NET技术社区较多的关注。

简介：Knowledgereuseisaprocessfordesigningnewproductbasedontheembeddeddesignknowledgeandexperienceofexistingproducts.Inordertoimprovequalityandefficiencyofproductdesign,amethodtoextractrelatedknowledgefromusingstandardpartsinproductdesignisintroduced.Basedonthecharacteristicsofstandardpartsandtheirapplication,knowledgereusablestandardpartslibrarywasbuiltupthroughanalysisofkeytechniquesofstandardpartmodelingsuchasknowledgeexpression,knowledgereuseandknowledgesearching.

简介：LivingLibrary服务2008年传入我国，次年在国内高校正式开展。但是，服务理念和方式极具人性化的LivingLibrary活动在国内并未形成规模效应。目前高校已开展的LivingLibrary服务局限性很大，主要由图书馆、大学生组织或院校科研机构举办，活动场所局限于图书馆内部；活动范围很小，仅有4所高校开展过此类服务项目；活动主题单一，涉及的社会问题有限，仅是国内个别高校服务创新的一种尝试。这一全新的服务理论被图书情报学界和广大用户普遍接受尚需时日。

简介：FemaleinflorescenceofBetulaplatyphyllawassampledatanintervalofeachtwodaystoanalyzethebackgroundofgeneexpressioninfloralphase.OnthebasisofSMARTstrategy,thedrivercDNAwasobtainedfromtotalRNAofthelastsampleandthetestercDNAwasfromthatoftheothersbyRT-PCRwhichweresubsequentlyusedtoconstructasubtractedcDNAlibrary.TheresultoftheESTs(expressionsequencetags)blastXshowedthatthegenesinthesubtractedcDNAlibrarycouldbemainlyclusteredinto5groupsrelatedtometabolism,transportationandsignaltransduction,cellcycle,stressresponse,andregulation.Therelationshipbetweengeneexpressionanddevelopmentwasalsodiscussed.

简介：为了学习简历，活跃代谢物由导出海绵的未耕作的共生者生产了，海绵Gelliodesgracilis的共生者的一个metagenomicDNA图书馆被构造。DNA的平均尺寸在图书馆插入是20kb。这个图书馆用纸圆盘assaying为抗菌素活动被屏蔽。二克隆对小球菌tetragenus显示了抗菌剂活动。这二克隆的代谢物通过HPLC被分析。结果证明他们的代谢物与主人E的那些相当不同。包含向量pHZ132的关口iDH5α和主人。这研究可以介绍一条新途径给探索简历海绵共生者的活跃代谢物。

简介：摘要对近几年国内外兴起的一种全新的图书馆服务方式与服务理念——HumanLibrary（原名LivingLibrary）的起源、特点、组织模式、发展现状、社会意义等方面做了全面的介绍与阐述，希望由此能引发更深入的研究，从而推动HumanLibrary的进一步发展。参考文献15。

简介：TherootofPanaxginsengplantundergoesaspecificdevelopmentalprocesstobecomeabiosynthesisandaccumulationtissueforginsenosides.Toidentifyandanalyzegenesinvolvedinthebiosynthesisofginsenoside,weconstructedandcharacterizedafull-lengthcDNAlibraryfor6-year-oldNorthAmericanginseng.ThetiterofprimarycDNAlibraryis1.2×106pfu/mL,thetiterofamplifiedlibraryis2.6×1010pfu/mLandtherateofrecombinantisabove86%.Theinsertsizerangesfrom0.3to2.0kb.Sequencingresultsshowthat18of58genesarehighhomologoustothegenes(GBR5,GBR3andGBR1)knowninGenBank,whichareinvolvedinbiosynthesisofginsenosideinNorthAmericanginsengplant;16of58genesarenovelgenes.Thefull-lengthcDNAlibraryofNorthAmericanginsengroottissuesisessentialforthecloningofgenesknownanditisalsoaninitialkeyforthescreeningandcloningofnewgenes.

简介：从Library2.0概念出发,对Web2.0及其相关技术进行了全面梳理,分析了Web2.0关键技术在Library2.0中的应用,探讨了Library2.0应用中存在的问题并提出了相应的对策。

简介：本文介绍Library2.0概念、服务理念及相关技术,调研国内外图书馆对Library2.0的实践情况,结合国家图书馆的实际情况,提出国家图书馆实践Library2.0的几点思路。

简介：ExtensiveexcavationsatthesiteofAshurallowedOlafPederséntoreconstruct,toaconsiderablelevelofdetail,themanyarchivesandlibrariesinofficialandprivatecollectionsthatexistedatancientAshurthroughthemiddle

简介：Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.