简介:Inthispaper,wepresentthecurrentstatusoftheprojectfordevelopingthenumericallibrayrinJAVA.Wehavepresentedhowobject-orientedtechniquesimproveusageandalsodevelopmentofnumericallibrariescomparedwiththeconventionalwayatpreviousconference,weneedmanyfunctionsfordataanalysiswhichisnotprovidedwithinJAVAlanguage,forexample,goodrandomnumbergenerators.specialfunctionsandsoon.Ourdevelopmentstrategyisfocusedoneasinessofimplementationandaddingnewfeaturesbyusersthemselvesnotonlybydevelopers.InHPCfiled,thereareotherfocuseffortstodevelopnumericallibrariesinJAVA,However,theirfocusisontheperformanceofexecution.noteasinessofextension.Followingthestrategy,wehavedegignedandimplementedmoreclassesforrandomnumbergeneratorsandsoon.
简介:DGPSL:ADISTRIBUTEDGRAPHICSLIBRARYShiJiaoying;PanZhigeng;ZhengWentingDGPSL:ADISTRIBUTEDGRAPHICSLIBRARY¥ShiJiaoying;PanZhigeng;...
简介:Knowledgereuseisaprocessfordesigningnewproductbasedontheembeddeddesignknowledgeandexperienceofexistingproducts.Inordertoimprovequalityandefficiencyofproductdesign,amethodtoextractrelatedknowledgefromusingstandardpartsinproductdesignisintroduced.Basedonthecharacteristicsofstandardpartsandtheirapplication,knowledgereusablestandardpartslibrarywasbuiltupthroughanalysisofkeytechniquesofstandardpartmodelingsuchasknowledgeexpression,knowledgereuseandknowledgesearching.
简介:FemaleinflorescenceofBetulaplatyphyllawassampledatanintervalofeachtwodaystoanalyzethebackgroundofgeneexpressioninfloralphase.OnthebasisofSMARTstrategy,thedrivercDNAwasobtainedfromtotalRNAofthelastsampleandthetestercDNAwasfromthatoftheothersbyRT-PCRwhichweresubsequentlyusedtoconstructasubtractedcDNAlibrary.TheresultoftheESTs(expressionsequencetags)blastXshowedthatthegenesinthesubtractedcDNAlibrarycouldbemainlyclusteredinto5groupsrelatedtometabolism,transportationandsignaltransduction,cellcycle,stressresponse,andregulation.Therelationshipbetweengeneexpressionanddevelopmentwasalsodiscussed.
简介:TherootofPanaxginsengplantundergoesaspecificdevelopmentalprocesstobecomeabiosynthesisandaccumulationtissueforginsenosides.Toidentifyandanalyzegenesinvolvedinthebiosynthesisofginsenoside,weconstructedandcharacterizedafull-lengthcDNAlibraryfor6-year-oldNorthAmericanginseng.ThetiterofprimarycDNAlibraryis1.2×106pfu/mL,thetiterofamplifiedlibraryis2.6×1010pfu/mLandtherateofrecombinantisabove86%.Theinsertsizerangesfrom0.3to2.0kb.Sequencingresultsshowthat18of58genesarehighhomologoustothegenes(GBR5,GBR3andGBR1)knowninGenBank,whichareinvolvedinbiosynthesisofginsenosideinNorthAmericanginsengplant;16of58genesarenovelgenes.Thefull-lengthcDNAlibraryofNorthAmericanginsengroottissuesisessentialforthecloningofgenesknownanditisalsoaninitialkeyforthescreeningandcloningofnewgenes.
简介:从Library2.0概念出发,对Web2.0及其相关技术进行了全面梳理,分析了Web2.0关键技术在Library2.0中的应用,探讨了Library2.0应用中存在的问题并提出了相应的对策。
简介:本文介绍Library2.0概念、服务理念及相关技术,调研国内外图书馆对Library2.0的实践情况,结合国家图书馆的实际情况,提出国家图书馆实践Library2.0的几点思路。
简介:ExtensiveexcavationsatthesiteofAshurallowedOlafPederséntoreconstruct,toaconsiderablelevelofdetail,themanyarchivesandlibrariesinofficialandprivatecollectionsthatexistedatancientAshurthroughthemiddle
简介:Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.