简介：OBJECTIVETodeservetheeffectsofFG(FibrinGlue)onendoscopicliposuction.Methods:FromOct.1998toAug.2000fortycaseswereundergoingfortheliposuction.Attheaidsoftumescentteachnique,endoscopyandFGwereused,ofwhich20casesoperationontheirabdominalwalls,8casesupperabdomenwallsand12casesownerabdominal.

简介：AbstractPlatelet-rich fibrin (PRF) is a second-generation platelet concentrate obtained from autologous blood. PRF is composed of abundant platelets, leucocytes, and a high concentration of various growth factors and fibrinogen. The composition and three-dimensional structure of PRF enable it to effectively make cells migrate and proliferate, playing an important role in tissue repair. Furthermore, the easy preparation and low cost of PRF make it a good treatment option. Numerous articles have been published about the application of PRF in clinical practice, however, the application of PRF in dermatology has not been comprehensively reviewed. The objective of this review article was to discuss various applications of PRF in dermatology, including healing chronic wounds, treating androgenic alopecia, skin rejuvenation, autologous fat transplantation, and treating vitiligo. PRF is a promising dermatologic treatment, but lacks a standardized protocol regarding its methods of attainment and use, which needs more investigations.

简介：Transcriptionfactor(TF)bindingtoitsDNAtargetsiteplaysanessentialroleingeneregulation.Thelocation,orientationandspacingoftranscriptionfactorbindingsites(TFBSs)alsoaffectregulatoryfunctionoftheTF.However,hownucleosomalcontextofTFBSsinfluencesTFbindingandsubsequentgeneregulationremainstobeelucidated.Usinggenome-widenucleosomepositioningandTFbindingdatainbuddingyeast,wefoundthatbindingaffinitiesofTFstoDNAtendtodecreasewithincreasingnucleosomeoccupancyoftheassociatedbindingsites.WefurtherdemonstratedthatnucleosomalcontextofbindingsitesiscorrelatedwithgeneregulationofthecorrespondingTF.Nucleosome-depletedTFBSsarelinkedtohighgeneactivityandlowexpressionnoise,whereasnucleosome-coveredTFBSsareassociatedwithlowgeneactivityandhighexpressionnoise.Moreover,nucleosome-coveredTFBSstendtodisruptcoexpressionofthecorrespondingTFtargetgenes.WeconcludethatnucleosomalcontextofbindingsitesinfluencesTFbindingaffinity,subsequentlyaffectingtheregulationofTFsontheirtargetgenes.ThisemphasizestheneedtoincludenucleosomalcontextofTFBSsinmodelinggeneregulation.

简介：A基于包含有选择地察觉到anionic客人种类的NH有约束力的地点的双能力的新比色的识别受体1被综合了。与另外的卤化物阴离子相比，它在dimethylsulfoxide的UV/Vis吸收光谱与高选择向氟化物阴离子的存在显示出反应，并且也显示了戏剧的颜色变化从对黄无色面对TBAF(5???搠灩票汬湩?汧'兮茨Κ桪

简介：Thebindingenergyofexcitonsconfinedtoaquantumringundertheinfluenceofperpendicularhomogeneousmagneticfieldiscalculatedasafunctionoftheringradius.Calculationsaremadebyusingthemethodofexactdiagonalizationwithintheeffective-massapproximation.Thefeatureofbindingenergyofthegroundstateasafunctionoftheringradiusforseveralvaluesofthemagneticfieldhasbeenrevealed.Theinterestingfeatureofourstudyisthat,inaquantumring,thegeometricstructureofexcitonsmayrevealtransition.

简介：DNAisthegeneticmaterialofvariousorganisms.ExtracellularDNAadsorbedorboundonsurface-activeparticlesinsoilshasbeenshowntopersistforlongperiodsagainstnucleasesdegradationandstillretaintheabilitytotransformcompetentcells.ThispaperreviewssomerecentadvancesonthebindingandtransformationofextracellularDNAinsoils,whichisfundamentaltounderstandingthenatureofthesoil,regulatingbiodiversity,andassessingtheriskofreleasinggeneticallyengineeredmicroorganisms(GEMs)aswellasbeinghelpfulfordevelopmentofthegeneticevolutionaltheoryofbacteria.Severalinfluencingfactors,suchassoilpH,ionicstrength,soilsurfaceproperties,andcharacteristicsoftheDNApolymer,arediscussed.Todate,theunderstandingofthetypeofmolecularbindingsitesandtheconformationofadsorbedandboundDNAtosoilparticlesisstillinitsinfancy.

简介：Versicanbelongstothefamilyofthelargeaggregatingchondroitinsulfateproteoglycanslocatedprimarilywithintheextracellularmatrix(ECM).Versican,likeothermembersofitsfamily,hasuniqueN-andC-terminalglobularregions,eachwithmultiplemotifs.Alargeglycosaminoglycan-bindingregionliesbetweenthem.Thisreviewwillbeginbyoutliningthesestructures,inthecontextofECMproteoglycans.Thediversebindingpartnersaffordedtoversicanbyvirtueofitsmodulardesignwillthenbeexamined.TheseincludeECMcomponents,suchashyaluronan,typeⅠcollagen,tenascin-R,fibulin-1,and-2,fibrillin-1,fibronectin,P-andL-selectins,andchemokines.VersicanalsobindstothecellsurfaceproteinsCD44,integrinβ1,epidermalgrowthfactorreceptor,andP-selectinglycoproteinligand-1.Thesemultipleinteractorsplayimportantrolesincellbehaviour,andtherolesofversicaninmodulatingsuchprocessesarediscussed.

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简介：ThebindingofCo(bpy)2dppz3+tocalfthymusDNAwasinvestigatedbyusingabsorptionandemissionspectroscopy,DNAmeltingtechniques,cyclicvoltammetry,viscosityandelectro-phoresismeasurements,wherebpyis2,2’-bipyridyl,dppzisdipyrido[3,2-o:2’,3’-c]phenazine.Thebindingcompoundshowsabsorptionhypochromicity,fluorescenceenhancement,andincreasingofDNAmeltingtemperatureandthespecificviscosity.CVmeasurementshowstheshiftsinoxidation-reductionpotentialandchangeinpeakcurrentwithadditionofDNA.ThecompoundisalsoshowntobemoreefficientphotosensitisersforstrandbreaksinplasmidDNA.

简介：探索真核细胞的抄写因素(TF)的性质有约束力的地点并且决定他们怎么不同于包围DNA序列，我们检验了与DNA有约束力的地点联系的四个特征：G+C内容，模式复杂性，palindromic结构，和Markov定序订。我们规章的主题的分析从TRANSFAC数据库获得了，用酵母内部是的遗传因子的序列背景，表明这四个特征在主题序列显示出可变丰富。例如，主题序列比是背景序列是更可能的有palindromic结构。另外，这些特征对规章的主题紧局部性，显示他们自己是主题序列的一个性质并且没被一般倡导者分享规章的主题在住的“环境”。由根据他们绑在的TF班毁坏主题序列，更特定的协会被识别。最后，当其它例如复杂性丰富，越过检验的种类是通用的时，我们发现一些关联例如G+C内容丰富，是种类特定的。这里提供的定量分析应该增加我们protein-DNA相互作用的理解并且也帮助通过生物信息学便于规章的主题的发现。

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简介：LipasewasimmobilizedbycovalentbindingoncrosslinkedallyldextranusingSESAascouplingagent.Itisshownthatthisimmobilizationapproachisanefficientoneforlipase.Theactivityoftheimmobilizedlipasecanreachto300-450U/g(dryweight).Itexhibitsgoodtemperaturestability,canretain88%activityafterbeingincubatedat70℃for2h.Specialeffectswillbeexpectedfromourimmobilizedlipaseinitsapplicationsinorganicmediaduetothenatureofthesupport.

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简介：这研究为预言并且分类氧绑定蛋白质描述一个方法。第一，支持向量机器(SVM)模块为预言氧绑定蛋白质用氨基酸作文和dipeptide作文被开发，并且分别地完成了85.5%和87.8%的最大的精确性。第二，一个SVM模块基于氨基酸作文被开发，分别地分类进有为erythrocruorin，蚯蚓血红蛋白，血蓝蛋白，血红素，腿血红素，和肌球素蛋白质的95.8%，97.5%，97.5%，96.9%，99.4%，和96.0%的精确性的六个班的预言的氧绑定蛋白质。最后，一个SVM模块为分类氧绑定蛋白质用dipeptide作文被开发，并且分别地为上述六个班完成了96.1%，98.7%，98.7%，85.6%，99.6%，和93.3%的最大的精确性。所有模块被五倍的生气确认训练并且测试。基于上述途径，网服务者Oxypred为预言并且分类氧绑定蛋白质被开发(从http://www.imtech.res.in/raghava/oxypred/可得到)。

简介：Thispresentstudyinvestigatedtheabilityofvarioussoyproteinhydrolysates(SPHs)inbindingcalcium.ItwasdemonstratedthattheamountofCa-bounddependedgreatlyontheSPHsobtainedusingdifferentproteases,whichincluded:neutrase,flavourzyme,proteaseMandpepsin.ThemaximumlevelofCa-bound(66.9mg/g)occurredwhenproteaseMwasusedtohydrolyzesoyprotein.PeptidefragmentsexhibitinghighCa-bindingcapacityhadmolecularweightsofeither14.4or8-9kDa.ThelevelofCa-boundincreasedlinearlywiththeincrementofcarboxylcontentinSPHs,andfurtherdeamidationonSPHsfromproteaseMimprovedCa-bindingofthehydrolysate.

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简介：Thestiffnessandstrengthofextracellular(EC)regionofcadherinareproposedtobetwoimportantmechanicalpropertiesbothforcadherinasamechanotransductorandfortheformationofcell-celladhesion.Inthisstudy,wequantitativelycharacterizedthestiffnessandstrengthofECstructurewhenitbindswithdifferenttypesofionsbymoleculardynamicssimulations.ResultsshowthatECstructureexhibitsarod-likeshapewithhighstiffnessandstrengthwhenitbindswiththebivalentionsofcalciumormagnesium.However,itswitchestoasoftandcollapsedconformationwhenitbindswiththemonovalentionsofsodiumorpotassium.ThisstudyshedslightontheimportantroleofthebivalentionsofcalciuminthephysiologicalfunctionofEC.

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