简介：Differentialproteinsexpressinginectopicandeutopicendometriawereinvestigatedbymeansofproteomicanalysis.Fivepatientsinsecretaryphasewerediagnosedasendometriosisbylaparoscopy.Thefiveectopicendometria(twoatstageⅡ,twoatstageⅢandoneatstageIV)andfiveeutopicendometriaweresurgicallyexcised.One-dimensionalelectrophoresiscoupledwithliquidchromatographyandmassspectrometrywasusedtoscreenandidentifydifferentialproteins.Threedifferentialbandsinone-dimensionalelectrophoresiswereresolvedbyliquidchromatographyandmassspectrometryand14up-regulatedproteinswereidentified,includingcollagenα-1,α-2,α-3(VI),α-1(XIV)chain,actin,annexinA2,EMILIN-1,ferritinlightpolypeptidevariant,fucosyltransferase10,myosin-9,proteinS100-A9,KIAA1783protein,andtwohypotheticalproteins.Ourdataprovidesalistofpotentialbiomarkersforendometriosis.Theidentificationsmaybeusedtodevelopnewdiagnosesforendometriosis.

简介：通讯因素分析(CFA)被采用学习14个HPLC系统的选择。测试LC系统作为反向阶段被分类(RP)，离子交换(IE)和吸水的相互作用层析(HILIC)模式。HILIC列上的吸水的溶质的保留被秒顺序效果显著地除他们的吸水的性质以外影响，这被发现。硅石表面上的器官的修饰词和残余silanol组两个都参予了保留。Hypersil？氨基的列以适当条件在HILIC模式执行了分离，并且它的保留机理比(aspartamide)HILIC，柱涂渍poly组织更类似于HILIC硅石列的。

简介：MtDNAwassuccessfullyextractedfromtenindividualbones(femurs)inthetombsofancientJushiinTurfanbasin,datedbacktotheyearabout3000-2500yearsago.Bymeansoffouroverlappingprimers,wegotnucleotidesequenceofthe218bplength.AncientmtDNAwasanalyzedbythesequencingofhypervariableregionⅠofthemtDNAcontrolregion.Theresultshowsthat9haplotypeswith24polymorphicsiteswereobtained.ThephylogeneticanalysisindicatedthatMongoliansandAltaiarethepopulationgeneticallyclosesttotheJushigroupsandJushimtDNApoolbeinganadmixtureofeasternAsianandEuropeanlineages.SoourpreliminarydataimplythatanancientminglingofEuro-AsianpopulationhadexistedinTurfanbasinpriortotheearlyIronAge.

简介：ThespinelLiMn2O4-x(SO4)xcompoundcathodematerialsweresynthesizedbysolid-statereactionofthecalculatedamountsofLiOH·H2O,MnO2andMnSO4.Theresultsoftheelectrochemicaltestdemonstratedthatthesematerialsexhbitedexcellentelectrochemicalproperties.Thehighestreversiblecapacityoftheseseriesofcathodematerialswas-120mAh/g,andafter50cycles,thisreversiblecapacitywasstillaround116mAh/gwithnearly100%reversibleefficiency,whichrevealedthatdopedsulfateioncouldimprovethestructuralstabilityofspinel.

简介：ThehydrogenbondpercentageanditstemperaturedependenceofthethreeTPUsamplessynthesizedfrompolytetrahydrofuran,4,4-diphenylmethanediisocyanate,N-methyldiethanolamineor1,4-butanediolwerestudiedbymeansofIRthermalanalysis.TheenthalpyandtheentropyofthehydrogenbonddissociationweredeterminedbytheVantHoffplot.

简介：Combinedwithflow-injection(Fl)technology,asimplechemiluminescence(CL)methodwasdevelopedforhydrazinedeterminationinthispaper.ItwasfoundthathydrazinecouldgreatlydecreasethestrongCLsignalproducedbythereactionbetweenluminolandhexacyanoferrate(Ⅲ)inalkalinemedium.ThedecreasedCLintensitywaslinearwithhydrazineconcentrationintherangeof5.0×10-9gmL-1to4.0×10-5gmL1,andthelimitofdetectionwas2.0×10-9gmL-1(3σ)witharelativestandarddeviationof2.4～4.1%(n=5).

简介：使用x射线衍射法，通过石棉的2口特征峰进行判断，分析滑石粉中可能存在的石棉成分。结果表明，样品中不含温石棉、铁石棉和青石棉，并对温石棉和绿泥石进行了有效区分。方法快速，灵敏，可有效用于滑石粉中石棉的测定。

简介：ＡＰＰＬＩＣＡＴＩＯＮ　ＯＦ　ＷＡＸＤ　ＡＮＤ　ＳＡＸＳ　ＴＯ　ＳＴＲＵＣＴＵＲＡＬ　ＡＮＡＬＹＳＩＳ　ＯＦ　ＰＡ１０１０＋ＢＭＩＡＰＰＬＩＣＡＴＩＯＮＯＦＷＡＸＤＡＮＤＳＡＸＳＴＯＳＴＲＵＣＴＵＲＡＬＡＮＡＬＹＳＩＳＯＦＰＡ１０１０＋ＢＭＩ￥Ｈｏｎ...

简介：Arapidandeffectivemethod,solidphaseextractioncoupledwithhighperformanceliquidchromatography(SPE-HPLC),wasappliedtotheseparationandanalysisofginsenosides.WatersOASISHLBwasusedforconcentratingandpurifyingsamplesandAlltimaC_(18)(53mm×7mm,3μm)chromatographycolumnwasusedforseparatingginsenosideRg_1,Re,Rb_1,Rc,Rb_2andRd.Theseginsenosideswereanalyzedwithin20mininourgradientelutionprocessandtheequilibriumtimeofthechromatographycolumncostonly5min.Moreover,therewasnoobviousbaselinedriftinourexperiment.Thismethodwasusedtoanalyzethecontentsofginsenosidesindifferentginsengproductsforqualitycontrol.Fourginsengproductswerestudied,includingtwokindsofcapsules,onekindoftabletandonekindofinjection.Theresultsshowthatthemethoddevelopedinthispaperhadgoodaccuracy,linearityandprecision.Therefore,thismethodcouldbeappliedinqualitycontrolofginsengproducts.

简介：

简介：ThesynthesisofanewtypeofX-ray-sensitivecompound'di-(1-hydroxylundecyl)diselenide'anditsapplicationinthepreparationofanewtypeofliposomewithX-raysensitivitywasreported.Thisnewliposomewassynthesizedtoencapsulatedoxorubicinhydrochloride(Dox),withitsphysicalandchemicalproperties,stability,andradiationsensitivitydetermined.BasedonthepH-gradientmethod,liposomalDoxwaspreparedviaultrasonicemulsificationandthenpurifiedonaSephadexG50mini-column.UVspectrophotometryandliquidchromatographywereusedtodetecttheencapsulationefficiencyandradiationsensitivityoftheDox-loadedliposome.Theresultsshowthatthroughchangesinreleaserate,thisliposomeshowsarelativeradiosensitivity.Intermsofradiationsensitivity,thedrugleakrateoftheX-ray-sensitiveDox-loadedliposomeincreasedgraduallyandpeakedat65.4%undertheX-rayradiationofadoseof10Gyormorethan10Gy,whichissignificantlydifferentfromthatofordinaryliposomes.Meanwhile,X-ray-sensitiveDox-loadedliposomehasagooddispersionstability,withanaverageparticlesizeofapproximate120nm.TheefficiencyofthisliposomeencapsulatingDoxwas75.84%,slightlylowerthanthatofordinaryliposomes.TheX-ray-sensitiveDox-loadedliposomeexhibitedsuspensionstabilitywithin30dofstorageat4°C,withoutvisibleprecipitation.Di-(1-hydroxylundecyl)diselenideissafeandnoncytotoxicandcomparedwiththoseofsyntheticphospholipidsitssynthesisislowcostanddoesnotrequirecomplexconditions.

简介：应用波长色散X射线荧光光谱法对中低合金钢、电工钢、高锰钢、不锈钢中锰元素进行测定。采用经验系数法校正元素间的增强-吸收效应和光谱重叠干扰,元素检测限为2.1μg/g,线性相关系数大于0.9998。考察了分析方法的精密度和准确度,元素的测定结果与认定值一致,相对标准偏差小于2%。

简介：建立了X射线荧光光谱法测定矿石样品中铀、钍含量的快速分析方法。采用高压粉末制样法,对不同含量的放射性样品的压片压力、粒径、含水率、用量等处理条件到进行单因素实验。在400MPa压力下压制,克服了低压制样的弊端,制备的样片表面光滑、致密,大幅改善了制样重现性,有效地减少了部分基体效应,铀校准曲线的标准偏差从0.053%降到0.0071%,钍校准曲线的标准偏差从0.062%降到0.0057%。经国家一级标准物质验证,表明方法准确、可靠,能满足样品中铀、钍含量日常分析要求。

简介：分别研究了采用压片、熔片两种制样方法，用x射线荧光光谱法（XRF）测定了红土镍矿中的9种元素。压片法重点研究了基体效应校正，经散射线作内标和经验系数法校正后，可准确测定除二氧化硅、氧化镁外的7种元素，方法简便、快速；而熔片法着重研究了熔剂和熔样温度的选择，经基体效应校正，各分析元素的结果准确度完全可与化学法相媲美，其相对标准偏差（RsD）在0．50％-3．00％间。

简介：以Li_2B_4O_7、LiBO_2和LiF（质量比为45∶10∶5）为混合熔剂,NH_4NO_3为氧化剂,LiBr为脱模剂,熔融制作样片,采用硅质砂岩、石英岩标准样品和配制标准样品作为校准样品,建立了熔融制样-X射线荧光光谱法（XRF）测定硅石中主次量成分（SiO_2、Al_2O_3、TFe_2O_3、MgO、CaO、K_2O、MnO、TiO_2、P_2O_5）的快速分析方法。对样品制备以及分析测试过程中的条件进行了优化,在最优条件下,对标准样品（GBW03112、GBW07835）进行重复测定,相对标准偏差RSD〈2%。同时对3个混合配制的硅石标准样品进行分析,结果与参考值无显著性差异。

简介：建立了采用X射线荧光光谱法测定液体水玻璃中的Al2O3、CaO、MgO、Fe2O3、K2O、Na2O、SiO2含量的方法。研究了适合直接测定液态水玻璃的制样方式、仪器分析参数,确定了工作曲线并录入数据库中。对未知样品的测定结果与化学法、电感耦合等离子体原子发射光谱（ICP-AES）法测定结果对比分析,证实了方法具有良好的准确度。精密度实验显示,方法的相对标准偏差（RSD）在0.38%-14.3%。结果显示方法的准确度、精密度完全可以满足对水玻璃中主次成分测定的要求。

简介：Thedye-dopedsilicananoparticlescanbeusedasnanobiosensorsthatareabletorecognizeanddetectspecificDNAsequence.Inthispaper,sphericalnanosizedluminol/SiO2compositeparticleshavebeensynthesizedwithreversemicellsviahydrolysisoftetraethylorthosilicate(TEOS)inthemicroemulsion.ThenanoparticlesweremodifiedwithchitosanandusedtolabelDNA,formingtheDNAprobewhichwasusedtohybridizewithtargetDNAimmobilizedonaPPymodifiedPtelectrode.Thehybridizationeventswereevaluatedbyelectrogeneratedchemiluminescence(ECL)measurementsandonlythecomplementarysequencecouldformadouble-strandedDNA(dsDNA)withDNAprobeandgivestrongECLsignals.Athreebasemismatchsequenceandanon-complementarysequencehadalmostnegligibleresponses.Duetothelargenumberofluminolmoleculesinsidesilicananoparticles,theassayallowsdetectionatlevelsaslowas2.0×10^-12mol/LofthetargetDNA.TheintensityofECLwaslinearlyrelatedtotheconcentrationofthecomplementarysequenceintherangeof5.0×10^-12—1.0×10^-9mol/L.

简介：InanefforttoutilizeNMRtechniquefortheanalysisofthestructuralcomponentsofwoodandbiomassessuchaslignin,wemadeacomparativestudyoflignincontentsofseveraltypesoflignocellulosicmaterialsbothbeforeandafterenzymatichydrolyses.Tothisend,EucalyptusGlobulus,Norwaysprucethermomechanicalpulp(TMP)cornstoverandEucalyptuskraftpulpwereball-milledandtreatedwithcellulasetopreparematerialswithhighlignincontents.Thesematerialswereanalyzedvia31PNMRinionicliquids.Theresultsshowthatthecontentsofvariousfunctionalgroupsforthelignocellulosicmaterialsafterenzymatichydrolysesaregenerallyinagreementwiththosefromtheircorrespondingenzymaticmildacidolysisliginins(EMALs),indicatingthat31PNMRanalysisoflignocellulosicmaterialsinionicliquidsisapromisingmethodforquantitativecharacterizationoflignocellulosicmaterials.

简介：药的效果的研究，kanamycin，在recombinantEscherichiacoli的生长新陈代谢上，B1被对待的细胞的新陈代谢的活动监视的microcalorimeter执行。种recombinantEscherichiacoli房间暂停的力量时间曲线，与不同kanamycin剂量对待，被记录。效果的程度被变化在microcalorimetric曲线的斜坡评估，药行动的动力学从这些变化在到达了他们的最大的价值并且维持了他们的最大的价值的时间被解释。试验性的剂量效果关系遵循集体行动的法律的中部效果的原则：fa/(1?fa)=(D/D50)m。y=lg的一个阴谋[(fa)1?1]?1对x=lgD给斜坡m，D50和R。试验性的结果表明kanamycin的高集中在lg阶段在recombinantEscherichiacoliB1的生长上有禁止的效果，并且在静止时期有支持的效果。而且，microcalorimetry是为在生物学的调节效果的察觉的一个可靠方法，这被表明。

简介：采用熔融制样，以钾长石、钠长石国家标准样品为主，辅以粘土、炉渣、硅石等标准样品，配制成一系列校准标准样品，用X射线荧光光谱法测定钾长石和钠长石中的Na20、A1203、Si02、K20、CaO、Fe2O2、TiO2的含量，结果表明：方法的相对标准偏差为0．065％-2．9％，其测定结果与标准值基本一致，准确度好，可替代湿法化学分析，适合于长石类硅铝酸盐矿物的日常分析。