简介：目的：建立SYBRGreenⅠ实时荧光PCR定量检测人POT1基因的方法。方法：依据人POT1基因及参照基因TBP基因序列设计引物，以人类乳腺癌细胞系MCF-7总RNA逆转录合成的cDNA为模版，以TBP基因为参照基因，应用SYBRGreenⅠ实时荧光定量PCR检测POT1基因的表达。结果：POT1和TBP基因熔解曲线为单峰，无杂峰及二聚体。POT1基因及参照基因TBP基因扩增效率相同（直线斜率0．0171〈0．1）。POT1基因批内变异与批间变异分别为1.3％和2.1％；TBP基因批内变异与批间变异分别为1．2％和2．6％。结论：建立了SYBRGreenⅠ实时定量检测人类POT1基因的方法，该方法简单快速，经济，灵敏度高，特异性和重复性好，可用于人类POT1基因的定量分析。

简介：Asimpleone-potnon-isocyanaterouteforsynthesizingthermoplasticpolyureasispresented.Insituurethanizationwasconductedfromthering-openingreactionofethylenecarbonatewithpoly(propyleneglycol)bis(2-aminopropylether)andhexanediamine,m-xylylenediamine,ordiethyleneglycolbis(3-aminopropyl)etherat100℃for6hundernormalpressure.Melttransurethanepolycondensationwassuccessivelyconductedat170℃underareducedpressureof399Pafordifferenttimeperiods.Aseriesofnonisocyanatethermoplasticpolyureas(NI-TPUreas)wereprepared.TheNI-TPUreaswerecharacterizedbygelpermeationchromatography,FTIR,1H-NMR,differentialscanningcalorimetry,thermogravimetricanalysis,wide-angleX-raydiffraction,atomicforcemicroscopy,andtensiletest.NI-TPUreasexhibitedMnofupto1.67×10^4g/mol,initialdecompositiontemperatureover290℃,andtensilestrengthofupto32MPa.SeveralcrystallizableNI-TPUreasexhibitedTmexceeding98℃.NI-TPUreaswithgoodthermalandmechanicalpropertieswerepreparedthroughagreenandsimpleone-potnon-isocyanateroute.

简介：小浪变换成功地在在本地时间与他们的能力处理的地震数据被使用了—频率分析。然而，因为小浪变换系数揭示仅仅三空间取向，directionality的鉴定被限制。而山脉让，变换为与线性地改变特征处理信号的方向察觉和能力有一个优异能力。在这篇论文，我们在场基于山脉处理的地震数据让的低signal-to-noise比率(SNR)的问题转变。有从华南的低SNR的实际地震数据用让的山脉被处理了改进SNR和地震事件的连续性的变换。结果证明让的山脉转变比对这些任务的小浪变换好。

简介：我们包括double-phonon-resonance报导分布式的反馈量串联激光(DFBQCL)的放射激光的性质活跃区域，在大约8.4亩m的波长。国外获得光谱由于在注射者在活跃区域和层次在更低的激光水平之间联合被产生，并且被其宽度是在1.5次阀值水流的0.5亩m的相应Fabry-PerotQCL的放射激光的光谱表明。作为结果，采用不同栅栏时期的DFBQCL在各种各样的热水池温度在房间温度和0.28亩m的全部的波长范围展出0.18亩m的波长跨度。当激光在搏动的模式在房间温度操作，大约30dB和1.78kA/cm(2)的低阀值水流密度的Ahigh方面模式抑制比率被完成。

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简介：Thispaperconsidersaperturbedrenewalriskprocessinwhichtheinter-claimtimeshaveaphase-typedistributionunderathresholddividendstrategy.Integro-differentialequationswithcertainboundaryconditionsforthemoment-generatingfunctionandthemthmomentofthepresentvalueofalldividendsuntilruinarederived.Explicitexpressionsfortheexpectationofthepresentvalueofalldividendsuntilruinareobtainedwhentheclaimamountdistributionisfromtherationalfamily.Finally,wepresentanexample.

简介：Lidarisaneffectivetoolforremotelymonitoringtargetorobject,butthelidarsignalisoftenaffectedbyvariousnoisesorinterferences.Therefore,detectingtheweaksignalsburiedinnoisesisafundamentalandimportantprobleminthelidarsystems.Inthispaper,aneffectivenoisereductionmethodcombiningwaveletimprovedthresholdwithwaveletdomainspatialfiltrationispresentedtodenoisepulselidarsignalandisinvestigatedbydetectingthesimulatingpulselidarsignalsinnoise.Thesimulationresultsshowthatthismethodcaneffectivelyidentifytheedgeofsignalanddetecttheweaklidarsignalburiedinnoises.

简介：在认知无线电(CR)系统，察觉到的有效光谱保证第二等的用户(苏)成功地存取光谱洞。典型地，察觉问题从传播策略的优化独立被考虑了。在实践，由于失误察觉和虚惊的非零可能性，然而，察觉到的阶段影响产量象在主要用户(PU)的传播上一样怀疑。在这份报纸，用精力察觉，我们最大化全部的产量由联合在multibandCR系统优化察觉阀值和传播策略怀疑。一套重复基于算法被建议解决这个混合整数编程问题，它与IEEE802.22标准建议的一致察觉阀值选择算法相比显示出更好的性能。

简介：Apre-filtercombinedwiththresholdself-learningwaveletalgorithmisproposedforhydraulicpressuresignalsdenoising.Thedenoisingthresholdisself-learntinthesteadyflowstate,andthenmodifiedunderagivenlimittomakethemeansquareerrorsbetweenreconstructionsignalsanddesirableoutputsminimum,sothecorrespondingoptimaldenoisingthresholdinasingleoperatingcasecanbeobtained.Theseoptimalthresholdsareusedforthewholesignaldenoisingandaredifferentinvariouscases.Simulationresultsandcomparativestudiesshowthatthepresentapproachhasanobviouseffectofnoisesuppressionandissuperiortothoseoftraditionalwaveletalgorithmsandback-propagationneuralnetworks.Italsoprovidestheprecisedataforthenextstepofpipelineleakdetectionusingtransienttechnique.

简介：Theshiftinthepercolationthresholdofcompressedcompositeswasstudiedbya3Dcontinuumpercolationmodel.AMonteCarlo(MC)methodwasemployedinthesimulations.Thepercolationthresholdwasfoundtorisewiththecompressionstrain,whichcapturesthebasictrendincompression-inducedconductivityvariationfromtheexperiments.Bothfiberbendingandtextureformationcontributetothepercolationthreshold.Theresultssuggestthatfillerswithahighaspectratioaremoredesirableforsensorandelectricalswitchapplications.

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简介：Thereliabilityoftheproductsoldunderawarrantyisusuallymaintainedbythemanufacturerduringthewarrantyperiod.Aftertheexpiryofthewarranty,however,theconsumerconfrontsaproblemabouthowtomaintainthereliabilityoftheproduct.Thispaperproposes,fromtheconsumer’sperspective,areplacementpolicyaftertheextendedwarranty,undertheassumptionthattheproductissoldundertherenewablefreereplacementwarranty(RFRW)policyinwhichthereplacementisdependentontherepair-costthreshold.Theproposedreplacementpolicyisthereplacementaftertheextendedwarrantyisperformedbytheconsumerbasedontherepair-costthresholdorpreventivereplacement(PR)age,whicharedecisionvariables.Theexpectedcostratemodelisderivedfromtheconsumer’sperspective.Theexistenceanduniquenessoftheoptimalsolutionthatminimizestheexpectedcostrateperunittimeareoffered.Finally,anumericalexampleispresentedtoexemplifytheproposedmodel.

简介：Basedonthenon-Darcyflowcharacteristicsofsurfactantfloodinginthelowpermeabilityoilfield,consideringthechangesofthresholdpressureandinfluenceofsurfactantonconvection,diffusion,adsorptionandretention,amathematicalmodelisestablishedforathree-dimensional,two-phase,three-componentsurfactantflooding.Anewtreatmentforthechangesofthresholdpressureandanovelcorrectionmethodfortherelativepermeabilitycurveintheprocessofsurfactantfloodingarederived,whichenhancesthematchingdegreebetweenthemathematicalmodelandfieldpractice.ThemathematicalmodelwasusedtoperformthenumericalsimulationstudyforapilottestofsurfactantfloodinginChao45BlockofDaqingOilfield,aproperinjectionplanwasoptimized.Aftertheoptimizedplanwascarriedoutinoilfield,thedesirableeffects,likepressure-reducing,injectionrateincrease,andtheincreaseofoilrecovery,wereachieved.Theaverageoilincreaseforsinglewellreaches37%,theratioofcosttorevenueisabove1:4,sotheeconomiceffectofscaleispromising.

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简介：Wedemonstrateanultra-low-thresholdphononlaserusingacoupled-microtoroid-cavitysystembyintroducinganovelcouplingapproach.Theschemeexhibitsbothhighopticalqualityfactorsandhighmechanicalqualityfactors.Wehaveexperimentallyobtainedthemechanicalqualityfactorupto18,000invacuumforaradialbreathingmodeof59.2MHz.Themeasuredphononlasingthresholdisaslowas1.2μW,whichis～5timeslowerthanthepreviousresult.

简介：自适应解调是一种新的传输速率自适应方案,一般都需要结合无率码来使用,以解决解调中的比特删除问题。一种自适应解调方案是计算已调制符号中比特的对数似然比,与预设的解调门限相比较来决定是否解调该比特。解调门限决定了调制的阶数,并对无率码的译码性能有较大影响。文章对结合LT码的自适应解调中解调门限的设置进行研究,找到最优的解调门限。仿真结果表明,在最优门限下,可获得最低的误码率,或者获得最高的传输效率。

简介：基于流场界面厚度（Interfacial—Fluid—Thickness，IFT）理论，建立了高折射率梯度门限模型来研究气动光学窗口光传输畸变。首先在光学窗口折射率梯度场基础上，提出高折射率梯度门限，忽略绝对值低于该门限的折射率梯度值，重构折射率场，并对其气动光学传输效应进行仿真。结果表明，当58．37％的梯度值被忽略时，得到的重构折射率场与原折射率场仿真光程差（OPD）最大相对误差不超过1．5％，验证了气动光学窗口高折射率梯度区域是产生光传输畸变的主要原因，也证实了该门限模型对气动光学窗口光传输效应进行仿真的可行性，对气动光学失真的机理、预测及校正有一定的指导意义。

简介：在我国利率市场化与金融改革不断推进的宏观背景下,商业银行风险承担与收益之间的关系是值得研究的重要课题。首先基于做市商模型剖析银行利率风险和违约风险承担对净利差的影响机理,进而以中国16家商业银行2008—2015年的财务数据为基础,构建交互项模型和非线性动态面板门限模型,并分析两者的非线性关系的存在性和作用机制。研究发现,利率风险承担和违约风险承担与净利差之间分别存在着反N型和倒U型的非线性关系。