简介：Astheprogressofvascularsurgery,artificialvesselshavebecomethesubstituteforlargeandmiddlediametervesselsbuthavenotforsmalldiameteronesowingtothrombogenesisandocclusionwithinashortperiodoftimeafterbeingapplied.ArtificialvesselendothelializationisoneoftheidealmethodstoresolvesuchissueandhasbeenimprovedcontinuouslysinceHerring1in1978putforwardthisterminthefirsttimeandutilizedvascularendothelialcells(ECs)harvestedfromlivinganimalstoperformthetestofartificialvesselendothelialization.

简介：ObjectiveTo观察激活p21的kinase6(PAK6)在在成年rat.MethodsSprague-Dawley老鼠的针的绳索损害(SCI)以后的表示和它的可能的角色受到针的绳索损害。为了探索PAK6，表示模式和PAK6的分发的病理学、生理的意义，没被西方的污点观察，免疫组织化学和immunofluorescence.ResultsWestern污点分析证明PAK6蛋白质水平在白天2和白天4上是显著地起来调整的，然后减少了并且有到白天14为止的起来规定。免疫组织化学分析证明PAK6的表示显著地在与控制组相比的白天4上被增加。而且，染色的两倍immunofluorescence证明PAK6首先在控制组在神经原和星形细胞被表示。当时在损害以后，PAK6的表示在星形细胞显著地被增加，神经原，和星形细胞大部分被增殖。我们也检验了增殖的房间的表示原子抗原(PCNA)并且发现它的变化与PAK6的表示被相关。重要地，在在受伤针的绳索的PAK6的injury.ConclusionThe起来规定可以与glial增长被联系以后，染色的两倍immunofluorescence表明PCNA评估的房间增长在白天4上出现在许多PAK6-express-ing房间。

简介：Objective:Toobservehumanneuronalapoptosissecondarytotraumaticbraininjury,andtoelucidateitsregulativemechanismandthechangeofexpressionofapoptosis-relatedgenes.Methods:Specimensofbrainwerecollectedfromcasesoftraumaticbraininjuryinhumans.Thehistologicalandcellularmorphologywasexaminedbylightandelectronmicroscopy.TheextentofDNAinjurytocorticalneuronswasdetectedbyusingTUNEL.ByinsituhybridisationandimmunohistochemistrythemRNAchangesandproteinexpressionofBcl-2,Bax,p53,andcaspase3p20subunitwereobserved.Results:Apoptoticneuronsappearedfollowingtraumaticbraininjury,peakedat24hoursandlastedfor7days.Innormalbraintissueactivatedcaspase3wasrare,butashorttimeaftertraumaitbecameactivated.Theactivitypeakedat20-28hoursandremainedhigherthannormalfor5-7days.TherewasnoexpressionofBcl-2mRNAandBcl-2proteininnormalbraintissuebut8hoursafterinjurytheirexpressionbecameevidentandthenincreased,peakedat2-3daysandremainedhigherthannormalfor5-7days.TheprimaryexpressionofBax-mRNAandBaxproteinwashighinnormalbraintissue.At20-28hourstheyincreasedandremainedhighfor2-3days;onthe7thdaystheyreturnedtoanormallevel.Innormalbraintissue,p53mRNAandP53wereminimallyexpressed.Increasedexpressionwasdetectedatthe8thhour,anddecreasedat20-28hoursbutstillremainedhigherthannormalonthe5thday.Conclusions:Followingtraumaticinjurytothehumanbrain,apoptoticneuronsappeararoundthefocusoftrauma.ThemRNAandproteinexpressionofBcl-2,Baxandp53andtheactivityofcaspase3enzymeareincreased.