简介:CaF2:Ho3+/Yb3+nano-particleswithintensegreenup-conversion(UC)luminescencearesuccessfullysynthesizedviaafacilehydrothermalapproachbyusingNH4FasthefluoridesourceandNa2EDTAasachelatingreagent.PowderXraydiffraction(XRD),transmissionelectronmicroscopy(TEM),fieldemissionscanningelectronmicroscopy(FE-SEM),andUCemissionspectraareusedtocharacterizethestructures,shapes,andluminescentpropertiesofthesamples.TheeffectsfromfluoridesourcesandchelatingreagentsontheformationsofCaF2nano-particlesareinvestigated,andtheformationprocessisalsodeduced.Undertheexcitationofa980-nmlaserdiode,thesampleseachshowagreenup-conversionemissioncenteredat540nmcorrespondingtothe5S2/5F4→5I8transitionsofHo3+.Moreover,theUCmechanismsofHo3+/Yb3+co-dopedCaF2nano-particlesarealsodiscussed.
简介:Foundlingislandsarealsocalledbabysafetyislands.Overseastheyarealsocalledfoundlingprotectioncabinsandareaplacetocareforabandonedbabies.InJune2011,theShijiazhuangSocialWelfareInstituteinHebeiProvincesetupafoundlingisland.Bytheendof2013,manycitieshadsetupsuchislands,whichisanewmeasureforensuringchildrenrights.Therearedifferentopinionson
简介:摘要目的应用无血清培养基分离U2OS人骨肉瘤肿瘤干细胞,并鉴定相关肿瘤标志物表达。方法体外培养人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞,免疫荧光检测U2OS人骨肉瘤肿瘤干细胞球中CD105表达。采用免疫印迹法检测人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞Nanog和Oct4蛋白表达。采用半定量PCR检测人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞SOX2、Nanog和Oct4mRNA的表达。结果荧光显微镜下可见,U2OS人骨肉瘤肿瘤干细胞球中部分细胞CD105呈阳性表达。免疫印迹检测结果显示人骨肉瘤U2OS细胞株的Nanog和Oct4蛋白相对表达量为0.853±0.404和0.603±0.153,均明显低于U2OS人骨肉瘤肿瘤干细胞0.973±0.305和0.827±0.322(均p<0.05)。半定量PCR检测结果显示U2OS人骨肉瘤肿瘤干细胞SOX2、Nanog和Oct4mRNA的相对表达量为0.190±0.027、0.178±0.025、0.170±0.021,均明显高于人骨肉瘤U2OS细胞株0.032±0.009、0.086±0.016、0.019±0.006(均p<0.05)。结论人骨肉瘤U2OS细胞株中存在肿瘤干细胞,无血清培养可用于分离培养人骨肉瘤肿瘤干细胞。
简介:Inthispaper,weconsiderthefollowingchemotaxismodelwithratio-dependentlogisticreactiontermu/t=D▽(▽u-u▽ω/ω)+u(α-bu/ω),(x,t)∈QT,ω/t=βu-δω,(x,t)∈QT,u▽㏑(u/w)·=0,x∈Ω,00,x∈,w(x,0)=w0(x)>0,x∈,Itisshownthatthesolutiontotheproblemexistsgloballyifb+β≥0andwillblowuporquenchifb+β<0bymeansoffunctiontransformationandcomparisonmethod.Variousasymptoticbehaviorrelatedtodifferentcoefficientsandinitialdataisalsodiscussed.
简介:MicroRNAs(miRNAs)areendogenoussmallnon-codingRNAsthatrepresstheirtargetsatposttranscriptionallevel.ExistingstudieshaveshownthatmiRNAsareimportantregulatorygenesinhepatocellularcarcinoma(HCC),aseithertumorsuppressorsoroncogenes.MiR-122isnormallydownregulatedinHCCandregardedasatumorsuppressor.RecentlymiR-122hasbeenreportedtoberegulatedbyCEBPA,whichistheninvolvedinanovelpathwaytoinfluenceproliferationoftumorcells.HoweveritisunknownwhetherCEBPAisregulatedbymiRNAsinHCC.Inthisstudy,wefindthatmiR-182isupregulatedinHCCmodelrat,andrepressesCEBPAinbothratandhuman.ThisfurtherimprovesthecurrentCEBPA/miR-122pathwaythatcontrolstheproliferationoftumorcells.TheseresultssuggestthatmiR-182isapotentialoncogeneinHCCandcouldbeusedasadiagnosticmarkeranddrugtargetofHCC.
简介:U.S.sanctionsonIranhaveexpandedsincethestartoftheIraniannuclearissue.Theyhavegrownfromsmall-scaleembargoestotoday’ssanctionsonmultiplesectorsincludingfinance,trade,energy,andpersonnel.DuringU.S.PresidentBarackObama’stenure,thereisverylittlelikelihoodthattheU.S.willresorttoforce,leavingWashingtonwithnoothermeansthansanctionstopersuadeIrantochangeitsnuclearpolicy.Thismeansthatalthoughsanctionshavealimitedeffect,theU.S.willcontinuetoprimarilyrelyonthemtoforceIran’shand.
简介:Aconvergingpointhasemergedonthehorizon,withanincreasingnumberofcallsforbuildingChina-U.S.+XmechanismsamongChinesescholars,andaveritablezealforinitiatingaROK-U.S.-ChinadialoguefromPresidentParkGeun-hyeofSouthKorea.Thispaperintendstoexplorethepossibilityofsuchatrilateraldialogueandcooperationarrangement,byanalyzingthebackgroundtothetrilateralrelations,theirrespectivestanceandconsiderations,aswellasanyhurdlesinvolvedintheprocess.
简介:土壤水保留曲线(SWRC)提供在弄干与吸以浸透,水内容或空比率的度的变化弄湿过程的/期间描述不饱和的土壤的反应的一个重要工具。在产生这些曲线的关键考虑是怎么测量吸。经常,过滤器纸技术被采用,特别当高吸被开发时,例如与塑料泥土。当每大小与这种技术花至少一个星期,在弄干并且弄湿产生完整的SWRC能花月或年。在实验室张力计的开发现在允许矩阵吸直到要测量的大约1.5MPa。与如此的一台设备,由连续测量在小时或天为象淤泥和泥土那样的小粒的土壤开发SWRC是可能的。这份报纸描述被开发在三小粒的土壤弄干期间在体积,水内容和矩阵吸测量变化的试验性的安装。仪器的限制和曲线的实用性被讨论。
简介:Thestudyreportsacaseofa5-year-oldChinesegirldiagnosedwithKabukimake-upSyndrome(KMS).ThepatientshowedclassicKMSappearance:widelyseparatedeyes,ectropionoflateralone-thirdlowereyelids,flatnasaltip,andprominentears.Auditoryfeaturesinthisindividualincludedbilateralseveresensorineuralhearinglossandlackof40HzAERPresponsesidentifiedatIyearofage.Theindividualreceivedcochlearimplant(CI)intheleftearwhen5yearsold.andrehabilitationafterCItreatmentwere3inspeechintelligibilityand5inauditoryperformance.Thus,ourfindingssuggestthatcochlearimplantmaybehelpfultorestorehearingforindividualswithKabukisyndrome.