学科分类
/ 1
14 个结果
  • 简介:CleavageofchromosomalDNAintooligonucleosomalsizefragmentsisanintegralpartofapoptosis.ElegantbiochemicalworkidentifiedtheDNAfragmentationfactor(DFF)asamajorapoptoticendonucleaseforDNAfragmentationinvitroGeneticstudiesinmicesupporttheimportenceofDFFinDNAfragmentationandpossiblyinapoptosisinvivo.RecentworkalsosuggeststheexistenceofadditionalendonucleasesforDNAdegradation.Understandingtherolesofindividualendonucleasesinapoptosis,andhowtheymightcoordinatetodegradeDNAindifferenttissuesduringnormaldevelopmentandhomeostasis,aswellasinvariousdiseasedstates,willbeamajorresearchfocusinthenearfuture.

  • 标签: 细胞凋亡 DNA断裂 核酸内切酶
  • 简介:<正>Apoptosisisahighlyregulatedphysiologicalprocesscriticalindevelopmentandtissuehomeostasis.Abnormalapoptosiscanleadtodiseaseconditionsincludingneurodegeneration,autoimmunityandcancer.DNAfragmentationisanintegralpartofapoptosisandhaslongbeensuspectedtobeofcriticalimportanceincleaninguppotentiallyantigenicDNAandgeneticmaterialcapableofinducingneoplasmictransformationinneighboringcells.DirectevidenceforthisfunctionofDNAfragmentationhowever,isstilllacking.TheidentificationofaheterodimericDNAfragmentationfactor45and40(DFF45andDFF40,alsocalledICADforInhibitorofCaspaseActivatedDNaseandCADforCaspaseActivatedDNaserespectively)aswellas

  • 标签: 细胞凋亡 DNA断裂 体内稳态
  • 简介:Apoptosiscanbetriggeredbyavarietyofstimuliincludingdeathfactors,anti-cancerdrugsandfactor-deprivation.Theseapoptoticcellsareswiftlyphagocytosedbymacrophagestopreventthereleaseofnoxiousorinflammatorymaterialsfromdyingcells.ThemolecularanalysisofFasligand(adeathfactor)-inducedapoptosisindicatedthatacascadeofproteases(caspases)isactivatedduringthisprocess,whicheventuallyactivatesaspecificDNase(caspase-activatedDNase).CADexistsasacomplexwithitsinhibitor(ICAD)inproliferatingcells.Whenthecellsaretriggeredtoapoptosis,caspases,inparticularcaspase3,inthedownstreamofthecaspasecascadecleaveICAD,whichreleasesCADtocauseDNAdegradationinnuclei.

  • 标签: 细胞凋亡 蚕噬作用 染色体DNA损毁
  • 简介:Eelfamilyisahugeone,inwhichmanykindsofeelsespeciallysomemigratoryeels,bearstrongresemblancetoeachother,andarethereforedifficulttobeidentified.Inthisstudy29randomprimerswereusedtomakeRAPDanalysisforJapaneseseel(Anguillajaponica),Europeaneel(Anguillaanguilla)andPikeeel(Muraenesoxcinereus).Andtotally299fragmentswerecounted.Sharedorspecificfragmentswerecountedandgeneticsimilarityorgeneticdistancewerecalculated.ThegeneticsimilaritybetweenJapaneseeelandPikeeelis0.68andthegeneticdistancebetweenthemis0.32;thosebetweenEuropeaneelandPikeeelare0.72and0.28respectively,andbetweenJapaneseeelandEuropeaneelare0.74and0.25respectively.Themethodhasbeenshowntobesuitabletomolecularidentificationofeels.Itprovidesanalternativeapproachtodeterminetherelationshipbetweenspecies.

  • 标签: 随机扩增多态性DNA分析 鳗鱼 种类 遗传距离 分类
  • 简介:在源于mitochondrial机能障碍的氧化phosphorylation的改变长被假设了涉及tumorigenesis。线粒体最近被显示了在调整规划房间死亡和房间增长起一个重要作用。而且,mitochondrialDNA(mtDNA)变化在各种各样的癌症房间被发现了。然而,在tumorigenesis的这些mtDNA变化的角色仍然保持大部分未知。这评论集中于基本mitochondrial遗传,mtDNA变化和与癌症联系的结果的mitochondrial机能障碍。潜在的分子的机制,调停从mtDNA变化的致病和到tumorigenesis的mitochondrial机能障碍也被讨论。

  • 标签: 线粒体DNA突变 功能障碍 程序性细胞死亡 分子机制 氧化磷酸化 MTDNA
  • 简介:Humanpolymorphonuclearleukocytes(PMN)havebeenreportedtocompletelylackofDNA-dependentproteinkinase(DNA-PK)whichiscomposedofKuproteinandthecatalyticsubunitDNA-PKcs,neededfornonhomologousend-joining(NHEJ)ofDNAdouble-strandbreaks.PromyelocyticHL-60cellsexpressavariantformofKuresultinginenhancedradiationsensitivity.ThisraisesthequestioniflowefficiencyofNHEJ,instrumentalforthecellularrepairofoxidativedamage,isanormalcharacteristicofmyeloiddifferentiation.HereweconfirmedthecompletelackofDNAPKinPMNproteinextracts,andtheexpressionofthetruncatedKu86variantforminHL-60.However,thisdegradationofDNA-PKwasshowntobeduetoaDNA-PK-degradingproteaseinPMNandHL-60.Inaddition,byusingaprotease-resistantwholecellassay,bothKu86andDNA-PKcscouldbedemonstratedinPMN,suggestingthepreviouslyreportedabsenceinPMNofDNA-PKtobeanartefact.ThelevelsofKu86andDNA-PKcsweremuchreducedinPMN,ascomparedwiththatofthelymphocytes,whereasHL-60displayedamarkedlyelevatedDNA-PKconcentration.Inconclusion,ourfindingsprovideevidenceofreduced,notdepletedexpressionofDNA-PKduringthematurestagesofmyeloiddifferentiation.

  • 标签: 粒细胞 人类 蛋白激酶 基因修复 表达 非同源末端连接
  • 简介:TounderstandtheDNA-methylationmediatedgenesilencingmechanisms,weanalyzedincellcultureofthepromoterfunctionoftheMAGE-A1gene,whichisfrequentlydemethylatedandover-expressedinhumanhepatocellularcarcinoma.WehaveestablishedthecorrelationoftheDNAmethylationofthepromoterCpGislandwithexpressionstatusofthisgeneinapaneloftheestablishedlivercancercelllines.ThecrucialCpGdinucleotide(s)withintheminimalpromotersubjectedtothecontrolmediatedbyDNAmethylationwithprofoundbiologicalfunctionswasalsodelineated.Furthermore,anovelsequence-specificDNA-proteininteractionatthe-30CpGdinucleotideupstreamofthegenewasfoundhavingavitalparttoplayintheDNAmethylationmediatedtranscriptionsilencingoftheMAGE-A1gene.OurresultswouldnotonlyprovidenewinsightsintotheDNAmethylationmediatedmechanismsovertranscriptionoftheMAGE-A1gene,butalsopavethewayforfurtherdefiningthecross-talkamongDNAmethylation,histonemodificationandchromatinremodelingindetail.

  • 标签: 二核苷酸 甲基化 转录 基因沉默 蛋白质 脱氧核糖核酸
  • 简介:Meiotic前期我是一个长、复杂的阶段。相应再结合是在meiotic前期期间发生在相应染色体之间的一个重要过程我。chiasmata的形成,它一起保持相应染色体直到中期我到后期,我转移,为合适的染色体分离是批评的。最近的研究建议了SPO11蛋白质在产生被认为是相应再结合的起点的双strandedDNA裂缝(DSB)的地点在很多个有机体保存了功能。DSB的这些地点处理要求RecA相当或相同的事物的功能,例如RAD51,DMC1,和其它,由变异的研究建议了;因此,修理这些meioticDSB的失败导致反常chromosomal引申,导致破坏成熟分裂。这些RecA相当或相同的事物的功能上的最近的发现改进了位于meiotic下面的机制的理解相应再结合。

  • 标签: 双股DNA 基因功能 细胞分裂 染色体
  • 简介:RecognitionofDNAdamageisacriticalstepforDNAdamage-mediatedcellularresponse.XPCisanimportantDNAdamagerecognitionproteininvolvedinnucleotideexcisionrepair(NER).WehavestudiedtheXPCproteinincisplatinDNAdamagingtreatment-mediatedcellularresponse.ComparisonofthemicroarraydatafrombothnormalandXPCdefectivehumanfibroblastsidentified861XPC-responsivegenesinthecisplatintreatment(withminimumfoldchange≥1.5).Thecellcycleandcellproliferation-relatedgenesarethemostaffectedgenesbytheXPCdefectinthetreatment.Manyothercellularfunctiongenes,especiallytheDNArepairandsignaltransduction-relatedgenes,werealsoaffectedbytheXPCdefectinthetreatment.Tovalidatethemicroarraydata,thetranscriptionlevelsofsomemicroarray-identifiedgeneswerealsodeterminedbyanRT-PCRbasedrealtimePCRassay.TherealtimePCRresultsareconsistentwiththemicroarraydataformostofthetestedgenes,indicatingthereliabilityofthemicroarraydata.Tofurthervalidatethemicroarraydata,thecisplatintreatment-mediatedcaspase-3activationwasalsodetermined.TheWesternblothybridizationresultsindicatethattheXPCdefectgreatlyattenuatesthecisplatintreatment-mediatedCaspase-3activation.Weelucidatedtheroleofp53proteinintheXPCproteinDNAdamagerecognition-mediatedsignalingprocess.TheXPCdefectreducesthecisplatintreatment-mediatedp53response.TheseresultssuggestthattheXPCproteinplaysanimportantroleinthecisplatintreatment-mediatedcellularresponse.Itmayalsosuggestapossiblemechanismofcancercelldrugresistance.

  • 标签: 顺氯氨铂 蛋白质 细胞反应 脱氧核糖核酸损伤 治疗 微序列分析
  • 简介:Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.

  • 标签: 水稻 第4号染色体 DNA文库 LA-PCR 显微解剖
  • 简介:在Saccharomycescerevisiae,必要基因CDC13编码telomeric遗传上并且身体上与Stn1p和Ten1p交往的搁浅单人赛的DNA有约束力的蛋白质,并且为telomere结束保护和telomere长度控制被要求。Ten1由参予telomere长度规定和染色体结束保护的分子的机制留下逃犯。在这个工作,我们用净化的recombinantCdc13p和Ten1p在胶化过滤分析观察了Cdc13p和Ten1p的一个弱相互作用。Ten1p本身展出一项弱DNA有约束力的活动,但是提高telomericTG1鈥吗?Cdc13p的DNA有约束力的能力。Cdc13p是有Ten1p的co-immunoprecipitated。在变异的ten1-55或ten1-66房间,在Ten1p和Cdc13p之间的损害相互作用与telomericDNA导致长得多的telomeres,以及Cdc13p的一个减少的协会。一致地,Ten1-55和Ten1-66异种蛋白质没能刺激telomeric在vitro的Cdc13p的DNA有约束力的活动。这些结果建议Ten1p提高telomericCdc13p到的DNA有约束力的活动否定地调整telomere长度。

  • 标签: DNA结合活性 端粒长度 DNA结合蛋白 弱相互作用 端粒DNA 分子机制