简介:BackgroundTaxifolin(Tax)isanessentialnaturalantioxidant.MultiplestudieshaveshownthatTaxcanprotectcardiomyocytesfromischemia-reperfusioninjury.However,theunderlyingmechanismisstillunclear.MethodsH9C2cellswererandomlydividedintocontrol,H_2O_2group,Taxpretreatmentgroup(Tax+H_2O_2);Taxeffectgroup.CellactivitywasdetectedbyCCK-8andtheintracellularstructurewasobservedbytransmissionelectronmicroscopy.AutophagywasdeterminebyWesternblottinganalysisofBeclin-1,Bcl-2andPKC.ResultsTaxpretreatmentsignificantlyincreasedanti-apoptoticproteinBcl-2andautophagyproteinBeclin-1.ExpressionofPKCwasinhibitedbyTax.ConclusionsTaxpretreatmentcouldprotectH9C2cellsagainstH_2O_2-induceddamagethroughtheBcl-2andautophagypathways.
简介:Asanodematerialsforlithium-ionbatteries,SiO_2isofgreatinterestbecauseofitshighcapacity,lowcostandenvironmentalaffinity.AfacileapproachhasbeendevelopedtofabricateSiO_2@Chollowspheresbyhydrolysisoftetraethylorthosilicate(TEOS)toformSiO_2shellsonorganicspheretemplatesfollowedbycalcinationsinairtoremovethetemplates,andthentheSiO_2shellsarecoveredbycarbonlayers.ElectronmicroscopyinvestigationsconfirmhollowstructureoftheSiO_2@C.TheSiO_2@ChollowsphereswithdifferentSiO_2contentsdisplaygradualincreaseinspecificcapacitywithdischarge/chargecycling,amongwhichtheSiO_2@CwithSiO_2contentof67wt%exhibitsdischarge/chargecapacitiesof653.4/649.6mAhg~(-1)over160cyclesatcurrentdensityof0.11mAcm~(-2).TheimpedancefittingoftheelectrochemicalimpedancespectroscopyshowsthattheSiO_2@CwithSiO_2contentof67wt%hasthelowestchargetransferresistance,whichindicatesthattheSiO_2@Chollowspheresispromisinganodecandidateforlithium-ionbatteries.
简介:Anenvironmentallyfriendlyprecursor,adenosine,hasbeenusedasadualsourceofCandNtosynthesizenitrogen-dopedcarboncatalystwith/withoutFe.Ahydrothermalcarbonizationmethodhasbeenusedandwateristhecarbonizationmedia.Themorphologyofsampleswith/withoutFecomponenthasbeencomparedbyHRTEM,andtheresultshowsthatFecanpromotethegraphitizationofcarbon.Furtherelectro-chemicaltestshowsthattheoxygenreductionreaction(ORR)catalyticactivityofFe-containingsample(C–FeN)ismuchhigherthanthatoftheFe-freesample(C–N).Additionally,theintermediatesofC–FeNformedduringeachsyntheticprocedurehavebeenthoroughlycharacterizedbymultiplemethods,andthefunctionofeachprocedurehasbeendiscussed.TheC–FeNsampleexhibitshighelectro-catalyticstabilityandsuperiorelectro-catalyticactivitytowardORRinalkalinemedia,withitshalf-wavepotential20mVlowerthanthatofcommercialPt/C(40wt%).Itisfurtherincorporatedintoalkalinepolymerelectrolytefuelcell(APEFC)asthecathodematerialandledtoapowerdensityof100mW/cm~2.