简介:目的为了探索基质金属蛋白酶9(MMP9)在糖尿病视网膜病变(DR)进展中的潜在调节机制。方法利用Lipofectamine2000将pcDNA-MMP9质粒和pcDNA-Ang2质粒分别转染至原代大鼠视网膜Müller细胞(RMCs)中。分别利用MTT法与流式细胞仪检测细胞生存率与凋亡情况。同时探索了MMP9与血管紧张素2(Ang2)之间的交互作用。另外,RMCs分别在正常血糖水平与高血糖水平下用MMP9处理2天。此外,通过Western印迹测定细胞凋亡蛋白MMP9、Ang2、Bax2、Bcl2、聚腺苷二磷酸核糖聚合酶(PARP)降解产物、天门冬氨酸特异性半胱氨酸蛋白酶-3(caspase3)降解产物的表达水平。结果与对照组相比,siRNA-MMP9组细胞生存率显著增长而MMP9过表达组下降。与对照组相比,MMP9过表达组中细胞凋亡显著增加,而siRNA-MMP9组中则下降。MMP9表达由Ang2显着调节,而当MMP9表达改变时,Ang2表达无明显变化。再者,高血糖组中MMP9的表达显著性增加,而正常血糖组与对照组比较差异无统计学意义。另外,高血糖组中Bax2,Bcl2,PARP降解产物、caspase3降解产物的表达也显著增加,相应地对照组与正常血糖组则没有显著性改变。结论我们的研究结果表明,MMP9通过由Ang2调控或定位细胞凋亡相关蛋白(如Bax2,Bcl2,PARP降解产物、caspase3降解产物)诱导细胞凋亡,在DR进展中扮演着一个重要角色。
简介:为了解宁波市不同区域生猪感染猪链球菌2型的现状,我们在不同时间从宁波市不同区域采集了两种猪龄的病猪咽喉拭子、死猪扁桃体、屠宰场和鲜肉市场猪肉等四类251份生猪样品进行检测分析.样品经选择性增菌培养,采用荧光定量PCR方法检测猪链球菌2型cps2j、mrp和ef三种毒力基因,评估检测猪链球菌2型感染情况和菌株毒力风险.结果表明,不同区域样本中猪链球菌2型三种毒力因子检出率在病猪咽喉拭子中最高(53.2%、41.9%、40.3%),其次是死猪扁桃体样本;4—6月份病猪中猪链球菌2型检出率高达77.8%,且高毒力型猪链球菌2型菌株(cps2j+mrp+ef+)也大量检出(61.1%).说明春季是猪感染猪链球菌2型感染高发时节,尤其要做好防疫工作.
简介:Theeffectofλ/2SiO2overcoatonthelaserdamagecharacteristicsofHfO2/SiO2high-reflector(HR)coatingsisinvestigatedwith1-on-1andN-on-1laserdamagetestmethods.Thelaserdamagesurfaceof1-on-1isanalyzedbyastepanalyzer.ThesurfacemorphologiesshowthatlaserdamagemakesthecoatingdamagedareaprotrudentandroughforHRcoatingwithoutλ/2silicaovercoat,butconcaveandsmoothforHRcoatingwithλ/2silicaovercoat.Theresultof10-on-1multi-pulseirradiationonthesamepointofthecoatingshowsthatthereisanenergydensitystageonthedamagecurve.Ifthelaserenergydensityiswithintherangeofthestage,HfO2/SiO2HRcoatingswithλ/2silicaovercoatwillnotbedamagedmorethan2timesformulti-shots,andthesurfacedamagesareveryslightsothatthereisnoimpactonthecoatingperformance.Anotherinterestingresultisthattheenergydensitystageextendsfromthedamagethresholdtothepointofabout3timesofthreshold,whichissimilartotheeffectofthelaserconditiononcoating.
简介:Thenonlinearswitchingcharacteristicsoffusedfiberdirectionalcouplerswerestudiedexperimentally.Byusingfemtosecondlaserpulseswithpulsewidthof100fsandwavelengthofabout1550nmfromasystemofTi:sapphirelaserandopticalparametricamplifier(OPA),thenonlinearswitchingpropertiesofanullcoupleranda100%couplerweremeasured.Theexperimentalresultswerecoincidentwiththesimulationsbasedonnonlinearpropagationequationsinfiberbyusingsuper-modetheory.Nonlinearlossinfiberwasalsomeasuredtogettheinjectedpoweratthecoupler.Afterdeductingthenonlinearlossandinputefficiency,thenonlinearswitchingcriticalpeakpowersfora100%andanullfusedcouplerswerecalculatedtobe9410and9440W,respectively.ThenonlinearlossparameterPNinanexpressionofαNL=αP/PNwasobtainedtobePN=0.23W.
简介:Thepotentialenergycurves(PECs)ofBOmolecule,includingΣ+andΠsymmetrieswithdoubletspinmultiplicities,areobtainedemployingmulti-referenceconfigurationinteraction(MRCI)methodandDunning'scorrelationconsistentbasissets.Theanalyticalpotentialenergyfunctions(APEFs)arefittedusingtheMurrell-Sorbie(MS)functionandtheleastsquaremethod.BasedonthePECs,thespectroscopicconstantsofthestateshavebeendeterminedandcomparedwiththetheoreticalandexperimentalresultsavailabletoaffirmtheaccuracyandliabilityofthecalculations.Theroot-mean-square(RMS)errorsbetweenthefittedresultsandtheabinitiovaluesaretoolittleincomparisonwiththechemicalaccuracy(349.755cm-1).ItisshownthatthepresentAPEFsareaccurateandcandisplaytheinteractionbetweentheatomswell.ThepresentAPEFscanbeusedtoconstructmorecomplicatedAPEFordosomedynamicinvestigations.
简介:Usingamicrowavegenerator,chlorinedilutedbyheliumwasdissociatedtochlorineatomsthatsubse-quentlyreactedwithhydrogenazidetoproducetheexcitedstatesofNCl(a1△).Meanwhile,moleculariodinewithcarriergasofheliumreactedwithatomicchlorinetoproduceatomiciodinewhichthenwaspumpedtoexcitedstateofI(2P1/2)byanenergytransferreactionfromNCl(a1△).Inthispaper,thechangesofNCl(a1△)andNCl(bi∑)emissionintensityispresentedwhenI2/HeisintroducedintothestreamofCl/Cl2/He/HN3/NCl(a1△)/NCl(b1∑).ThedependencesofatomiciodineI(2P1/2)onflowratesofgaseswerealsoinvestigated.TheoptimumparametersforI(2P1/2)productionaregiven.
简介:新的一年,前几期来跟大家聊一聊一个“哲学”一点的问题:在交通领域,什么样的政策是好政策?从调控机制上来说,公共政策可以大概分为两类.一类是管制型(Regulatory)政策,另一类则是市场型(Market-based)政策.管制型政策即设立明确的标准,所有人都必须达到这一标准,比如在国五标准中,规定在工况测试中每辆车行驶一公里各种污染物的上限是多少,然后所有仍在销售的车型都必须在每一种污染物上都达标;市场型政策则是利用某种价格机制进行市场调节,根据外部成本/收益的大小,收取不同的费用,或者进行不同额度的补贴,比如燃油税就是典型的市场型政策,烧的油越多,就说明使用的道路资源越多,造成的污染、拥堵和道路损耗越多,从而鼓励人们少开车,或者购买更经济节能的车辆.