简介：WedevelopedanRfunctionnamed'microarrayoutlierfilter'(MOF)toassistintheidentificationoffailedarrays.Insortingagroupofsimilararraysbythelike-lihoodoffailure,twostatisticalindiceswereemployed:thecorrelationcoefficientandthepercentageofoutlierspots.MOFcanbeusedtomonitorthequalityofmi-croarraydataforbothtroubleshooting,andtoeliminatebaddatasetsfromdown-streamanalysis.Thefunctionisfreelyavaliableathttp://www.wriwindber.org/applications/mof/.

简介：MYB蛋白质在真核细胞的有机体起重要作用。在植物，R1R2R3类型MYB蛋白质在房间周期控制工作。然而，R2R3类型MYB蛋白质是否也涉及房间部门过程，仍然保持未知。这里，我们报导那R2R3类型抄写因素基因，AtMYB59，涉及房间周期前进和根生长的规定。AtMYB59蛋白质在洋葱的原子核是局部性的表皮的房间并且transactivation活动。在酵母房间的AtMYB59的表示压制房间增长，和transformants与更长的房间有更多的原子核和更高的aneuploidDNA内容。在AtMYB59的保存领域的变化在酵母细胞生长上废除它的效果。在同步Arabidopsis房间暂停，AtMYB59基因明确地在房间周期前进期间在S阶段被表示。表示和promoter-GUS分析表明AtMYB59基因富有地在根被表示。转基因的植物overexpressingAtMYB59更短的根与野类型的植物(Arabidopsis就职Col-0)相比，并且在在根尖端的有丝分裂的房间的一半附近在中期。相反地，空变异的myb59-1比关口在中期让更长的根和更少有丝分裂的房间，建议那AtMYB59可以由扩大有丝分裂的房间的中期禁止根生长。AtMYB59调整许多下游的基因，包括CYCB1；1基因，可能通过到MYB应答的元素的绑定。这些结果在细胞周期规定和植物根生长为AtMYB59支持一个角色。

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简介：5R618是高抗叶锈病小麦品系。为了确定该品系所携带的抗叶锈基因,以5R618与感病小麦品种郑州5389杂交获得F1,自交获得F2分离群体以及F2∶3家系,用叶锈菌生理小种THJP对亲本、F2分离群体以及F2∶3家系进行叶锈抗性鉴定,然后进行分子标记分析。结果显示,5R618对生理小种THJP的抗病性由1对显性基因控制,该基因暂命名为Lr5R。经过亲本和抗感池间分子标记筛选以及F2∶3家系的标记检测,Lr5R定位于染色体3DL上,barc71和STS24-16是Lr5R最近的2个标记,遗传距离分别为0.9cM和2.1cM。

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简介：目的：构建4E—BPl及其T37A、T46A、$65A、T70A突变体4E—BPl-4A基因表达的重组慢病毒载体，研究其对胃癌HGC27细胞生长的影响。方法：PCR扩增4E—BPl基因及其突变体aE—BPl-4A基因并克隆到pCDH载体，构建成pCDH-4E—BPl、pCDit一4E—BPl—4A，将其-9包装载体共转染293T细胞，包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，将此慢病毒感染胃癌HGC27细胞，Western印迹鉴定病毒载体介导的4E—BPl、4E～BPl—4A蛋白的表达，MTT、克隆形成和软琼脂方法研究过量表达4E—BPl、4E—BPl—4A对胃癌HGC27细胞生长的影响。结果：包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，并将此慢病毒载体感染胃癌HGC27细胞；MTT、克隆形成、软琼脂实验表明过量表达4E—BP!可抑制胃癌HGC27细胞的生长，过量表达4E—BP!一4A时抑制效果更明显。结论：构建了4E—BPl、4E—BPI-4A的重组慢病毒表达载体，在胃癌HGC27细胞中过量表达4E—BPl可抑制细胞生长，过量表达4E—BPl-4A的抑制效果更明显。

简介：Amajorproblemwhichispoorlyunderstoodinthemanagementofbladdercancerislowsensitivitytochemotherapyandhighrecurrenceaftertransurethralresection.Insulin-likegrowthfactor1receptor(IGF-1R)signalingplaysaveryimportantroleinprogression,invasionandmetastasisofbladdercancercells.Inthisstudy,weinvestigatedwhetherIGF-1Rwasinvolvedinthegrowthstimulatingactivityanddrugresistanceofbladdercancercells.Theresultsshowed:ThemRNAsofIGF-1,IGF-2andIGF-1Rwerestronglyexpressedinserum-freeculturedT24cellline,whereasnormalurothelialcellsdidnotexpressthesefactors/receptorsoronlyintracelevels;T24cellrespondedfarbettertogrowthstimulationbyIGF-1thandidnormalurothelialcells;blockageofIGF1Rbyantisenseoligodeoxynucleotide(ODN)significantlyinhibitedthegrowthofT24cellandenhancedsensitivityandapoptosisofT24cellstomitomycin(MMC).TheseresultssuggestedthatblockageofIGF-IRsignalingmightpotentiallycontributetothetreatmentofbladdercancercellswhichareinsensitivetochemotherapy.

简介：目的：通过检测藏獒黑素皮质激素受体1（MClR）基因的单链构象多态性（SSCP）在不同毛色群体中的分布，探讨MClR基因多态性与毛色表型的相关性。方法：采用DNA测序技术，选择不同毛色藏獒的DNA为样本，根据GenBank发布的荷斯坦牛MClR基因序列设计一对引物，采用PCR-SSCP技术分析MClR基因在藏獒中的SSCP。结果：MClR基因在藏獒中具有PCR-SSCP多态性，分别检测到3种基因型（AA、AB和BB）；对MClR基因多态性片段DNA克隆测序后发现，MClR基因在编码区第313位存在单碱基突变（G-，A），该突变导致第105位氨基酸发生由丙氨酸向苏氨酸的改变（T105A）。结论：肘CJR基因的多态性与毛色性状不存在显著的相关性。

简介：Mammaliancelltotipotencyisasubjectthathasfascinatedscientistsforgenerations.AlonglastingquestionwhethersomeofthesomaticcellsretainstotipotencywasansweredbythecloningofDollyattheendofthe20thcentury.Thedawnofthe218thasbroughtforwardgreatexpectationsinharnessingthepoweroftotipotentcyinmedicine.Throughstemcellbiology,itispossibletogenerateanypartsofthehumanbodybystemcellengineering.Considerableresourceswillbedevotedtoharnesstheuntappedpotentialsofstemcellsintheforeseeablefuturewhichmaytransformmedicineasweknowtoday.Atthemolecularlevel,totipotencyhasbeenlinkedtoasingulartranscriptionfactoranditsexpressionappearstodefinewhetheracellshouldbetotipotent.NamedOct4,itcanactivateorrepresstheexpressionofvariousgenes.Curiously,verylittleisknownaboutOct4beyonditsabilitytoregulategeneexpression.ThemechanismbywhichOct4specifiestotipotencyremainsentirelyunresolved.Inthisreview,wesummarizerethestructureandfunctionofOct4andaddresstoOct4functioninmaintainingtotipotencyorpluripotencyofembryonicstemcels.

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简介：冀豆4号是邯郸市农业科学院通过有性杂交选育出的集优质、高产、抗病和适应性广等优良性状于一体的大豆品种。该品种于1990年获得国家科技进步三等奖。利用冀豆4号及其衍生材料,通过杂交育种、诱变等手段,河北、山西、陕西3省育成了16个品种通过省审或国审,其中国审品种7个、高油品种5个,脂肪含量为21.79%～23.97%。育成的品种适宜我国黄淮和北方两大大豆主产区种植,在大豆生产中发挥了重要作用。冀豆4号在育种上的广泛应用,证明其不仅是一个优良品种,也是难得的优异种质。筛选和培育优异种质是大豆品种选育的前提,也是大豆研究的重要基础性工作。

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简介：InteractionbetweencytotoxicTlymphocyte-associatedantigen-4(CTLA4,CD152)andB7molecules(B7-1andB7-2)isofimportanceinthecellulareventsoflymphocyte,includingantigen-specificT-cellactivationandinductionofautoreactiveT-cell.WedescribehaerethefirstintroductionofamurinesolubleCTLA4gene,CTLA4Ig,toMm1cells,amacrophagiccellline.CTLA4IgwassuccessfullyexpressedonMm1cellsandtheexpressedCTLA4IgwasfoundtobefunctionallyactiveintheirbindingtoB7moleculesbyflowcytometryandimmunofluorescencestudies.ThebiologicalactivityofCTLA4IgfromthetransfectedMm1cellswasstudiedandshowedinhibitoryactivityonmixedlymphocyteculture.AhighCTLA4Igproducingmacrophagiccelllinewasobtained.AsMm1cellswereregardedasdifficultforgenetransfectionandtherehassofarbeennoreportonexpressionofCTLA4IggeneonMm1cells,theseresultssuggestedthattheCELA4IgexpressingMm1cellscouldbeusefulforanalysisofCTLA4andB8moleculeinteractioninbothmacrophageandT-cell.

简介：Trichosanthin(TCS)isapotentallergentomice.Accordingtoourpreviousexperiments,itcouldbringouttheIgEresponsetoovabumin(OVA)ifTCSwasgivenonedaybeforeOVAimmunization,whileOVAalonecouldnotinduceIgEtoit.Inthiswork,thekineticsofinterleukin4(IL-4)andinterferonγ(IFN-γ)geneexpressioninthemesentericlymphnode(MLN)ofTCS-immunizedmicewasinvestigatedusingasemi-quantitativeRT-PCRmethod.ItindicatedthatTCSinducedsignificantIL-4geneexpressionandthepeaksofIL4geneexpressionwereondayoneafterTCSimmunizationinbothprimaryandsecondaryresponse.Incontrast,theIFN-γgeneexpressionwassuppressed.Furthermor,theIL-4geneexpressioninthesecondaryresponsewaslowerthanthatintheprimaryresponse.ThusthepresenceofIgEmemoryBcellswerestudied.ResultsshowedthattheamountofmatureIgEmRNAarosesignificantlyandrapidlyonedayafterTCSrestimulation,whileintheMLNofthemiceprimed30daysbeforeandwithoutboost,itwasalmostasthesameamountoftheunimmunizedcontrol.ThesefindingssuggesttheexistenceoftheIgEmemoryBcellsinthemiceaftertheprimaryTCSimmunization.

简介：报道了内蒙古白粉菌4个新记录种，分别是寄生在白桦Betulaplatyphylla上的桦木白粉菌Erysiphebetulina、大果榆Ulmusmacrocarpa上的榆白粉菌原变种Erysipheulmivar．ulmi、刺果茶藤Ribesburejense上的醋栗单囊白粉菌Podosphaeramors-uvae和栾树Koelreuteriapaniculata上的栾树叉钩丝壳Sawadaeakoelreuteriae。其中，白桦Betulaplatyphylla和刺果茶蔗子Ribesburejense为上述白粉菌的国内新记录寄主，文中提供了详细的形态描述和线条图。引证标本保存在赤峰学院菌物标本室（CFSZ）。

简介：Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.

简介：记录枝孢属（CladosporiumLink）4个吉林省新记录种：连翘枝孢（C．forsytiaeZ．Y．ZhangetT．Zhang）、三宅枝孢（C．miyakeiSaccardoetTrotter）、柳枝孢（C．salicisMoseszetSmarods）、黑星状枝孢（C．venturicidesSaccardo）。文中对这些种分别进行了描述，并附有线条图。标本保存于吉林农业大学菌物标本室（HMJAU）。

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