简介：目的：研究手法对于颈本体觉紊乱的眩晕患者的头颅空间回复能力的影响。方法：利用头颅三维空间运动测量仪，临床观察72例颈本体觉紊乱的眩晕患者手法治疗前后其头颅空间回复能力的变化。另外选取51颈椎动脉供血不足的眩晕患者及138例正常人作对照组。结果：手法治疗后本体觉组在三个运动轴线上的头颅空间偏移度均有明显减小，结论手法有即刻改善颈本体觉功能的作用，在临床椎动脉供血不足型眩晕的发病也存在着本体觉紊乱的因素。

简介：ＴＨＥＥＦＦＥＣＴＯＦＲＥＴＩＮＯＩＣＡＣＩＤＯＮＣＥＬＬＭＥＭＢＲＡＮＥＡＮＤＭＥＴＡＳＴＡＴＩＣＡＢＩＬＩＴＹＯＦＭＯＵＳＥＦＯＲＥＳＴＯＭＡＣＨＣＡＲＣＩＮＯＭＡＣＥＬＬＹｕＸｉａｏｄａｎ于晓；ＺｈａｏＸｕｅｍｅｉ赵雪梅；ＧａｏＪｉｎ高进（Ｉｎ...

简介：为精子的准备的许多技术当前是可得到的，哪个通常采用的大多数是密度坡度centrifugation(DGC)和游泳起来(啜)。迄今为止，这些方法看起来在为帮助繁殖技术(艺术)选择功能的精子有效，但是他们可以在精子DNA上有否定效果。在这研究，处理技术消除单个海滨的包含的精子和双海滨DNA损坏的这些精液的能力被在157件精液样品的二尾巴的彗星试金和精子染色质分散测试从寻求帮助繁殖治疗的病人估计。我们显示的结果啜并且DGC在消除包含双海滨DNA损坏和精子与的精子是同等地有效的高度损坏了(降级)DNA由存在描绘了单个海滨并且双海滨DNA裂缝。然而，DGC比在选择从单个海滨的DNA损坏是免费的精子啜是更有效的。未来研究应该描述DNA损坏的各种各样的类型的重要性并且检验处理在每个实验室过去常决定他们的能力消除DNA损坏并且因此，经由艺术阻止基因变化的潜在的传播的协议的精子。

简介：组织一个链球菌(气体)，重要人的病原体，能引起包括表面的感染的感染并且潜在地的各种各样的类型致命的感染，和搜索因为阻止煤气的感染的一支有效疫苗是进行中的许多年了。这份报纸把FbaA(在气体的表面上表示的Fn有约束力的蛋白质)的immunogenicity和immunoprotection与M蛋白质的作比较，气体的最好的immunogen。为有免疫力的反应的试金显示出那FbaA，类似于M蛋白质，能在BALB/c老鼠导致蛋白质特定的高IgGtiter。而且，跟随煤气的挑战，与FbaA使免疫的老鼠与M显示出象那些的一样的保护的率蛋白质。这些结果显示FbaA处于在在老鼠对煤气的挑战导致保护的免疫的M蛋白质的能力是类似的。

简介：无

简介：

简介：Recently,ithasbeenreportedthattheluteolyticac-tionwasthemainmechanismoftheterminationofearlypregnancybycontraceptivest.Inaddition,someestro-genreceptorligandssuchasdroloxifenehavebeenshowntoinhibitthegrowthofculturedratlutealcells.Ithasbeenknownthat2-phenylindolederivativescouldcompetewithestradiolfortheestrogenreceptor.Theseresultspromptedustodesignandsynthesizethefollow-ingsixnew3-oxamoyl-2-phenylindolecompounds1a-fandtesttheireffectsonthegrowthoflutealcells,aimingatseekingnewleadcompoundspossessingantifertilityactivities.

简介：Bonemorphogeneticprotein(BMP)isanefficientbone-inducingproteinwhichmainlyexistsinbonematrix.TheimplantsofBovineBMP(bBMP)extractedfrombovinebonematrixcaninducedifferentiationofmesenchymalcellsofmicemusclein-tochondroandosteo-cytesandformnewbonefurther.Inordertoevaluateim-plantablebiomaterialthatpossessesnotonlyosteogenicability,butalsostengtn,

简介：AIM:JS-38(mitothiolore),asyntheticversionofametaboliteisolatedfromXenorhabdussp.,wasevaluatedforitsanti-tumorandwhitebloodcell(WBC)elevatingactivities.METHOD:Theseanti-proliferativeactivitieswereassessedinvitrousingapaneloftencelllines.Theanti-tumoractivitiesweretestedinvivousingB16allograftmousemodelsandxenograftmodelsofA549humanlungcarcinomaandQGYhumanhepatomainnudemice.Theanti-tumorinteractionsofJS-38andcyclophosphamide(CTX)or5-fluorouracil(5-Fu)werestudiedinaS180sarcomamodelinICRmice.SpecificstimulatoryeffectsweredeterminedonperipheralneutrophilsinnormalandCTX-and5-Fu-inducedneutropenicmice.RESULTS:TheIC50valuesrangedfrom0.1to2.0μmol·L-1.JS-38(1μmol·L-1)causedanincreaseinA549tumorcellapoptosis.Multi-dailygavageofJS-38(15,30,and60mg?kg-1?d-1)inhibitedinvivotumorprogressionwithoutasignificanteffectonbodyweight.JS-38additivelyenhancedtheinvivoanti-tumoreffectsofCTXor5-Fu.JS-38increasedperipheralneutrophilcountsandneutrophilratesinnormalBALB/cmicealmostaseffectivelyasgranulocytecolony-stimulatingfactor(G-CSF).InmicewithneutropeniainducedbyCTXor5-Fu,JS-38rapidlyrestoredneutrophilcounts.CONCLUSION:TheseresultssuggestthatJS-38hasanti-tumoractivity,andalsohastheabilitytoincreaseperipheralbloodneutrophils.

简介：AbstractObjective:This study was performed to investigate the relationship between the human melanogenesis and antioxidant systems and to further confirm the synergistic effect of oxyresveratrol (OXYR) and resveratrol (RES) in human epidermal melanocyte cell line.Methods:The human epidermal melanocyte line PIG1 cells were divided into the UV groups and control group, treated with different doses of UVB and without UVB, respectively. MTT assay and flow cytometry were used to detect cell viability and apoptosis. The expression of Nrf2/HO-1 and melanogenesis-associated proteins/genes was measured by Western blotting and real-time qPCR (RT-qPCR). pCMV6-XL5-Nrf2 was used to upregulate the expression of Nrf2. Subsequently, the proteins/genes levels of Nrf2/HO-1 and tyrosinase (TYR), melanin/eumelanin content, and reactive oxygen species (ROS) were analyzed. Isobologram analysis and cell experiment were used to analyze whether OXYR and RES inhibit TYR synergistically. Western blotting, RT-qPCR, and NaOH splitting method were used to determine the Nrf2/HO-1 and melanogenesis-associated proteins/genes expression and melanin content to evaluate the efficacy of OXYR and RES.Results:The activated Nrf2 and HO-1 eliminated ROS produced by UVB irradiation. The melanogenesis-associated proteins/genes of melanocyte-inducing transcription factor (MITF, P < 0.01 on protein expression), TYR (both P < 0.01), TYR-related protein (TRP)-1 (both P < 0.05), and TRP2 (P < 0.05 on mRNA expression) were activated in PIG1 cells by UVB irradiation. Simultaneously, the upregulation of Nrf2 significantly reduced melanogenesis formation (P < 0.001) and TYR level (P < 0.01 on protein expression). Moreover, OXYR and RES synergistically inhibited TYR activity (P < 0.001) and reduced melanin content (P < 0.001).Conclusions:A microbalance exists between Nrf2/HO-1 signaling and melanogenesis production in the UVB-induced responses of melanocytes. Simultaneously, OXYR enhances the ability of RES to inhibit melanin production.

简介：AbstractBackground:The CHA2DS2–VASc score was initially applied to stratify stroke risk in patients with atrial fibrillation (AF) and was found to be effective in predicting all-cause mortality outcomes. To date, it is still unclear whether circulating long non-coding RNAs (lncRNAs) as emerging biomarkers, can improve the predictive power of the CHA2DS2–VASc score in stroke and all-cause mortality.Methods:Candidate lncRNAs were screened by searching the literature and analyzing previous RNA sequencing results. After preliminary verification in 29 patients with AF, the final selected lncRNAs were evaluated by Cox proportional hazards regression in 192 patients to determine whether their relative expression levels were associated with stroke and all-cause mortality. The c-statistic, net reclassification improvement (NRI), and integrated discrimination improvement of the patients were calculated to evaluate the discrimination and reclassification power for stroke and all-cause mortality when adding lncRNA expression levels to the CHA2DS2–VASc score model.Results:Five plasma lncRNAs associated with stroke and all-cause mortality in AF patients were selected in our screening process. Patients with elevated H19 levels were found to have a higher risk of stroke (hazard ratio [HR] 3.264, 95% confidence interval [CI]: 1.364–7.813, P = 0.008). Adding the H19 expression level to the CHA2DS2–VASc score significantly improved the discrimination and reclassification power of the CHA2DS2–VASc score for stroke in AF patients. In addition, the H19 level showed a marginally significant association with all-cause mortality (HR 2.263, 95% CI: 0.889–5.760, P = 0.087), although it appeared to have no significant improvement for the CHA2DS2–VASc model for predicting all-cause mortality.Conclusions:Plasma expression of H19 was associated with stroke risk in AF patients and improved the discriminatory power of the CHA2DS2–VASc score. Therefore, lncRNA H19 served as an emerging non-invasive biomarker for stroke risk prediction in patients with AF.

简介：摘 要：开展巴林特小组可让医学生较早地认识到医患沟通的重要性和艺术性，能够及时发现自己在医患沟通中的盲点，养成自我反思的良好习惯，促进医学生换位思考培养同理心，获得医患沟通技巧指导，有助于自我效能的建立，并得到小组同伴支持有效缓解工作压力，促进医患关系改善带来的良性互动，本文从这8个方面对巴林特小组提升医患沟通能力的作用进行分析。在医学生培养方案、教学管理架构、小组组长（领导者）培训和激励机制4个方面提出有操作性的教学策略。