简介：Thelabellingandimagingoftumorcellswereinvestigatedviaarginine-glycine-asparticacidcysteine(RGDC)peptide-labelledquantumdots(QDs).TheresultsshowthatRGDCmodifiedQDscanlabelSMMC-7721tumorcellsandadheretocellularmembrane.Inconstrast,theunmodifiedQDsaremainlydispersedaroundthecell.WealsofoundthattheRGDC-QDscanpenetrateintothecellat2hofincubation.After6hofincubation,RGDC-QDscanaccumulateinauniqueintracellularregion.

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简介：Aseriesof5-aminolevulinicacidanditsalkylestermethanesulfonateswasexploitedtophotodynamictherapy(PDT)ofhumanlymphocyticcells,U-937invitro.ThePDTefficiencyisinfluencedbytheconcentrationandincubationtime.Generally,forALAanditsalkylestermethanesulfonates,thecellsurvivalratedecreasesandtheaccumulationabilityofPpIXincreaseswiththeconcentrationandincubationtime.Wefoundthatthelongercarbonchainmethanesulfonates(C5-S,C6-S,C8-S)exhibitbetterPDTeffectthanALAmethanesulfonate.ThispossiblyprovidesapromisingroutetotheclinicalapplicationofPpIX-mediatedPDTtocancercell.

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简介：Theauthorsfocusedtheirattentionontheestablishmentofamesenchymalstemcell(MSC)modelforscreeningtraditionalChinesemedicines(TCMs)soastoinvestigatetheeffectsofShuanglongFormula(SLF)components(Ginsenosidesandsalvianolicacids)andingredients(ginsenosideRb1andsalvianolicacidB)oncardiomyocytedifferentiationfromMSCs.TheSLFcomponentswereanalyzedandquantifiedbyHPLC-TOF-MS.CardiomyocytedifferentiationwasinducedbyculturingMSCsintheinductionmediumsupplementedwithSLFingredients,SLFcomponents,5-azacytidine(5-aza),5-aza+SLFingredientsand5-aza+SLFcomponents,respectively,forupto30d,andevulatedbytheexpressionofCardiac-specificmyosinheavychain(MHC)andtroponinI(TnI)viaimmunofluoresentstaining.Slowgrowthrateandchangedmorphologywereobservedduringcardiomyocytedifferentiation.After20dofinduction,differentiatingMSCswerepositiveforMHCandTnIstaining.TheeffectsofSLFcomponentswerebetterthanthoseofSLFingredients.Takentogether,SLFcaninducethedifferentiationofMSCsintocardiomyogeniccellsinvitro,andMSCscanbeusedasapowerfultoolforscreeningTCMs.

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简介：Nitricoxidehasplayedanimportantroleinmanyphysiologicalandpathologicalprocessesasakindofimportantgassignalmolecules.Inthiswork,anewfluorescentprobeLysoNO-NaphfordetectingNOinlysosomesbasedon1,8-naphthalimidewasreported.LysoNO-Naphhassub-groupsofo-phenylenediamineasaNOreactionsiteand4-(2-aminoethyl)-morpholineasalysosome-targetablegroup.Thisprobeexhibitedgoodselectivityandhighsensitivity(4.57mmol/L)towardNOinawidepHrangefrom4to12.Furthermore,LysoNO-NaphcanbeusedforimagingNOinlysosomesinlivingcells.

简介：AlantolactoneisanaturalcompoundidentifiedfromtherootsofInulaheleniumL.thathasmultiplebio-activities.Weexamineditsinhibitoryeffectsonhumannon-smallcelllungcancer(NSCLC)A549cells.Thean-tiproliferativeeffectofalantolactoneonA549cellswasinvestigatedviaMTT[3′-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide]assayanditsapoptosis-inducingeffectwasdeterminedbyHoechststainingandflowcytometry.WefoundthatalantolactonesignificantlyinhibitedtheproliferationofA549cellsandinducedmorphologicalchangestypicalforapoptosis.Flowcytometryanalysisindicatesdose-dependentcellcycleretardationatG0/G1andSstages.Theresultsindicatethatalantolactonecouldbeanattractivesmall-molecularnaturalcompoundforfurtherdevelopmentasatherapeuticdrugagainstNSCLC.

简介：从有水杨酸酸和thioproline的钐氯化物hexahydrate的反应的产品，[Sm(C7H5O3)2灭牥瑡牵?慷?楦瑴摥戠?敬獡?煳慵敲?敭桴摯?慂敳?湯琠敨映瑩整?潰祬潮業污?桴?浳潯桴摥栠慥?慣慰楣楴獥愠摮琠敨浲摯湹浡捩映湵瑣潩獮漠?桴?潣灭畯摮爠汥瑡癩?潴琠敨猠慴摮牡?敲敦敲据?整灭牥瑡牵?????眠牥?慣'褯N瑡摥愠摮琠扡汵瑡摥愠?‵?湩整癲污?吠敨挠湯瑳湡?潶畬敭攠敮杲?景挠浯畢瑳潩?景琠敨挠浯潰湵?瑡吠企??‵?慷?敭獡牵摥戠??牰'酞洕?硯杹湥戭浯?潣扭獵楴湯挠污牯浩瑥牥愠?啣????7敢?敤楬敶敲?景？

简介：为拳头预定准备一主要固态包括1-methyl-3-propylimidazolium碘化物(MPII)的高有效的电镀物品传导性的材料，benzimidazole(双性人)，碘和锂碘化物被报导。在这电解质，双性人充当不仅添加剂而且gelators。与它的重要电气化学的性质，3.07%的全面效率不到AM1.5被完成(100mW/cm2)。

简介：打印屏幕的nanoporousTiO2薄电影被采用制作敏化染料固态把CuI用作洞运输材料的太阳能电池。基于nanoporous，有大毛孔的TiO2薄电影由聚苯乙烯的增加形成了的太阳能电池与200nm的直径成球形到TiO2浆糊展览光电的表演改进，它与大毛孔在这部电影由于CuI的容易的穿入被归因于与敏化染料的薄电影的表面的CuI的好接触。

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简介：Theeffectandmechanismofcarmustine(BCNU)combinedwithall-transretinoicacid(ATRA)ontheapoptosisofhumanglioblastomaU251cellswereinvestigatedbymeansof3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazoliumbromide(MTT)assay,flowcytometry,reversetranscription-polymerasechainreaction(RT-PCR)andWesternblotanalysis.TheresultsshowthatBCNUorATRAshowstime-anddose-dependentinhibitioneffectsonhumanglioblastomaU251cellsandthecombinationofBCNUwithATRAshowsansynergisticinhibitioneffectonhumanglioblastomaU251cells,andthecombinedBCNUandATRAcansignificantlyinhibittheproliferationofhumanglioblastomaU251cells,andinducetheapoptosisofthem,makingthecellsarrestinthestageofG1phase,thestageofSandG2phasesdecline,therateoftheapoptosisofhumanglioblastomaU251cellsincrease,thecorrespondingmRNAexpressionofcyclinEandcyclin-dependentkinase2(CDK2)downregulatedandthecorrespon-dingmRNAexpressionofp27kip1unregulated.Inaddition,thecombinedBCNUandATRAreducedtheproteinexpressionofnuclearfactorkappaB(NF-κB).Takentogether,theseresultssuggestthatthetreatmentofhumanglioblastomaU251cellswithacombinationapplicationofATRAandBCNUcanexertsynergisticeffect,thecourseofthiskindofcombinationchemotherapymaylikelybeassociatedwithmultiplemolecularmechanismsforapoptosis,furthermore,thecyclinEandp27kip1shouldbeconsideredasnoveltargetsforcontrollingthegrowthofglioblastomacells.