Anovelcovalentlymodifiedglassycarbonelectrodewithβ-cyclodextrinwaspreparedviaelectropolymerizationtechniqueforthesimultaneousdeterminationofuricacid(UA),xanthine(XA),hypoxanthine(HX)anddopamine(DA).ThisnewelectrodepresentedanexcellentelectrocatalyticactivitytowardstheoxidationofUA,XA,HXandDAbycyclicvoltammetry(CV)method.Theoxidationpeaksofthefourcompoundswerewelldefinedandhadtheenhancedpeakcurrents.TheseparationpotentialsoftheoxidationpeaksforDA-UA,UA-XAandXA-HXwere150,390and360mVinCV,respectively.Bymeansofdifferentialpulsevoltammetry(DPV)method,thecalibrationcurvesintherangesof10―225,5―105,10―170and5―150μmol/LwereobtainedforUA,XA,HXandDA,respectively.Thelowestdetectionlimits(S/N=3)were5,1.25,5and1.5μmol/LforUA,XA,HXandDA,respectively.ThepracticalapplicationofthemodifiedelectrodewasdemonstratedbythedeterminationofDAinhydrochlorideinjectionandUA,XA,HXinhumanurinesamples.
