简介:BackgroundSepticshockcausedbybacteremiaisalife-threateninginfectionwhoseprognosisishighlydependentonearlyrecognitionandappropriatetreatment.Procalcitonin(PCT)hasbeenshowntoaccuratelyandquicklydistinguishbacteremiafromnoninfectiousinflammatorystatesincriticallyseverepatients.However,theextentofPCTmagnitudeelevationaccordingtotheGramstainresultinelderlypatientswithcoronaryheartdisease(CHD)attheonsetofsepticshockcausedbybacteremiavaries,andhasnotbeenclearlyelucidated.MethodsThemedicalrecordsofadvancedage(non-neutropenic)patientwithCHDandsepticshockbetweenMar2013andJun2015whohadbacteremiacausedbyeitherGram-positive(GP)bacteriaorGram-negative(GN)bacteriawerereviewed,andthelevelsofPCT,C-reactive(CRP)proteinandwhitebloodcellscount(WBC)inbothgroupswereanalyzed.Results75episodesofeitherGNbacteremia(n=40)orGPbacteremia(n=35)wereenrolled.PCTlevelswerefoundtobemarkedlyhigherinpatientswithGNbacteremiathaninthosewithGPbacteremia[(8.93±17.58)vs.(64.42±58.56)ng/L(P<0.001)],whereastherewasnosignificantdifferenceinCRPandWBC(P>0.05).Moreover,ahighPCTlevelwasfoundtobeindependentlyassociatedwithGNbacteremiainthisstudypopulation.APCTlevelof19.69ng/mLyieldeda72.5%sensitivity,a91.4%specificity,an8.43positivelikelihoodratioanda0.30negativelikelihoodratioforGN-relatedbacteremiainthestudycohort[AUROCC=0.870(0.041),95%CI(0.790-0.949)].ConclusionInanelderlypatient(non-neutropenic)withCHDandsepticshock,GNbacteremiacouldbeassociatedwithhigherPCTvaluesthanthosefoundinGPbacteremia(PCT>19.69ng/mL).
简介:Toexploretheprevalenceoftheplasmid-mediatedquinoloneresistancegeneqnrAinGram-negativebacteriaandtoinvestigateitsmoleculargeneticbackgroundandresistanceprofileinisolatesharboringthisgene,atotalof629nalidixicacid-resistantisolatesofnon-repetitiveGram-negativebac-teriawerecollectedfromclinicalspecimensbetweenApril2004andApril2006andtheseisolateswerescreenedforqnrAgenebyPCRusingspecificprimerscombinedwithDNAsequencing.Theextendedspectrumβ-lactamase(ESBL)orAmpC-producingisolatesweredistinguishedbythephenotypicconfir-matorytestcombinedwithDNAsequencing,andtheantibioticssusceptibilitytestforqnrA-positiveiso-lateswascarriedoutbyKirby-BauerandE-testmethod.TodetectthelocationoftheqnrAgene,plas-midconjugationandSouthernhybridizationwereperformedandtheintegronstructurecontainingtheqnrAgenewasclonedbyPCRstrategyandsequencedbyprimerwalking.ItwasdemonstratedthattheincidenceoftheqnrA-positivestrainsinnalidixicacid-resistantbacteriawas1.9%(12/629),inwhichthedetectionratesforKlebiesiellapneumoniae.Enterobactercloacae,Enterobacteraerogenes,CitrobacterfreundiiandSalmonellachoeraesuiswere2.2%(3/138),17.1%(6/35),9.1%(1/11),12.5%(1/8),and14.3%(1/7),respectively.TheqnrAgenewasfoundtobeembeddedinthecomplexsu/1-typeintegronlocatedonplasmidswithvariedsize(80-180kb).Amongthem,4qnrA-positiveisolatescarriedintegronIn37and8isolatescarriedanovelintegron,temporarilydesig-natedasInX.AlltheqnrA-positiveisolateswereESBL-producingandtransferableforthemuhi-drugresistance.Itisconcludedthattheplasmid-mediateddrug-resistancemechanismexistsinthequinoloneresistantstrainsofisolatesfromhospitalsinGuangdongarea,buttheincidencewasratherlow.Never-theless,itisstillpossiblethatthehorizontaltransferoftheresistantqnrAgenemightleadtothespreadingofdrug-resistance.
简介:AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.
简介:<正>现代汉语中,“不”字不能和体词性成分组合。不过前后同形的体词性成分之间可以加“不”字构成“N不N”格式。例如:(1)这关头,还有什么集体不集体!只能靠各自的直觉判断。(《十月》1984年第6期)(2)右派不右派与我什么相干!反右时我象海边远远一个小石子,浪花也没溅到我身上。(《小说选刊》1985年第6期)(3)甚么酒馆不酒馆,滚它的。(《契诃夫小说选》P43)(4)朱老忠说:“老英雄不老英雄,反正退回十几年去,有三五个人,走不到咱跟前。(梁斌《红旗谱》P304)本文从语法、语义、语用三个角度,粗略地分析“N不N”格式的特点。一“N不N”格式,根据一定的形式特征可以分为两大类:一类是多项“N不N”并列的形式,我们叫做A类;另一类是只有一项“N不N”的形式,我们叫做B类。我们先讨论A
简介:Itisnecessarytounderstandthebacterialpopulationsassociatedwithricesoastoprovidemoreinformationandnaturalresourcesforeffectivemanagementofmajordiseasesinrice.Asurveyonscreeningandidentificationofgram-positivebacteriawasconductedduring1998-2004.Sevenhundredandfifty-sixricesampleswerecollectedfromZhejiang,Jiangsu,FujianandYunnanProvinces,China.Over1000bacterialisolateswereisolatedandtestedforcolonymorphology,pathogenicity,andsomecharacteristicsofbacteriologyincludingGramstaining,fluorescentpigmentonKingsmediumBandmicroscopicobservationforendospore.Togetherwithfivestandardreferencestrains,74representativegram-positivebacterialisolateswereconfirmedbyBiologandgaschromatographicanalysisoffattyacidmethylesters.FivebacterialspeciesofBacillusandotherthreegenerawereidentifiedandisolatesfromBacillussublitisandBacillusmegaterium,exhibitedthemosteffectiveinhibitionagainstthepathogensofsheathblightandbakanaediseaseofrice.AfewisolatesfromBacilluspumilusandBacillusmegateriumshowedweakvirulentonricetogetherwithsomevirulentisolates,dskshouldbeconsideredwhenisolatesfromthesespecieswerescreenedforbiocontrolagents.
简介:摘要:本文首先阐述了湖南省YC高速公路公路桥梁工程项目中3个桥梁工程基本情况,参考《公路桥梁和隧道工程施工安全风险评估指南(试行)》(以下简称《指南》)中指标体系,针对具体案例对指标进行了选取,建立了影响公路桥梁工程总体安全风险的五大指标评价指标体系,并对风险评价指标进行权重赋值,构建了AHP-GRAM评价模型,根据具体案例分析与模型计算,证明了AHP-GRAM模型在公路桥梁工程总体安全风险评价中的可行性。
简介:对于方程Φ(n)=S(n11),Φ2(n)=S(n11)进行了研究,并得到了这两个方程的所有正整数解,其中Φ(n)为Euler函数,Φ2(n)为广义Euler函数,S(n)为Smarandache函数。