简介：Itiswidelyrecognizedthatexchange,distribution,andintegrationofbiologicaldataarethekeystoimprovebioinformaticsandgenomebiologyinpost-genomicera.However,theproblemofexchangingandintegratingbiologicaldataisnotsolvedsatisfactorily.TheeXtensibleMarkupLanguage(XML)israpidlyspreadingasanemergingstandardforstructuringdocumentstoexchangeandintegratedataontheWorldWideWeb(WWW).WebserviceisthenextgenerationofWWWandisfoundedupontheopenstandardsofW3C(WorldWideWebConsortium)andIETF(InternetEngineeringTaskForce).ThispaperpresentsXMLandWebServicestechnologiesandtheiruseforanappropriatesolutiontotheproblemofbioinformaticsdataexchangeandintegration.

简介：AuxindistributionduringembryogenesisandseedgerminationwerestudiedwithtransgenicArabidopsisplantsexpressingGUSgenedrivenbyasyntheticDR5promoter,anauxinresponsivepromoter.TheresultsshowedthatGUSactivityishigherinendsofhypophysisandcotyledonprimordiaofheart-,torpedo-andcotyledon-stageembryos,leaftiparea,lateralrootprimordia,rootapexandcotyledonofyoungseedlings.AndGUSaccumulatedinrootapexoftheseedlingsgrownonauxintransportinhibitorcontainingmedia.Allthesesuggestedthatabove-mentionedpartoftheorgansandtissueshaveahigherlevelofauxin,andauxinpolartransportinhibitorcouldcausetheaccumulationofauxininrootapex.AndauxintransportinhibitoralsoresultedinaberrationofArabidopsisleafpatternformation,rootgravitropismandelongation.

简介：Apoptosismanifestsintwomajorexecutionprogramsdownstreamofthedeathsignal:thecaspasepathwayandorganelledysfunction.Animportantantiapoptosisfactor,Bcl-2protein,contributesincaspasepathwayofapoptosis.Calcium,animportantintracellularsignalelementincells,isalsoobservedtohavechangesduringapoptosis,whichmaybeaffectedbyBcl-2protein.WehavepreviouslyreportedthatinHarringtonine(HT)inducedapoptosisofHL-60cells,there'schangeofintracellularcalciumdistribution,ovingfromcytoplastespeciallyGolgi'sapparatustonucleusandaccumulatingtherewiththehighestconcentration.Wereportherethatcaspase-3becomesactivatedinHT-inducedapoptosisofHL-60cells,whichcanbeinhibitedbyoverexpressionofBcl-2protein.NosignofapoptosisorintracellularcalciummovementfromGolgi'sapparatustonucleusinHL-60cellsoverexpressingBcl-2ortreatedwithAc-DEVD-CHO,aspecificinhibitorofcaspase-3.Theresultsindicatethatactivatedcaspase-2canpromotethemovementofintracellularcalciumfromGolgi'sapparatustonucleus,andtheprocessisinhibitedbyAc-DEVD-CHO(inhibitorofcaspase-3),andthatBcl-2caninhibitthemovementandaccumulationofintracellularcalciuminnucleusthroughitsinhibitiononcaspase-3.Calciumrelocalizationinapoptosisseemstobeirreversible,whichisdifferentfromtheintracellularcalciumchangescausedbygrowthfactor.

简介：粗纱甲壳虫类Dianous的96种沥滤，1819在中国被记录了。在这份报纸，我们描述新种，D。poecilusn。sp。从云南，和记录D。viriditinctus(冠军，1920)第一次从中国。便于种类，种类组或种类建筑群的鉴定，我们编了钥匙的一个新版本，包括迄今在中国的领土记录的Dianous的所有种类。而且，地理分发的主要模式为这个类的世界Dianous动物志被构画出。

简介：Twouncoupleabledistributions,assigningmissionstorobotsandallocatingrobotstohomestations,accompanytheuseofmobileservicerobotsinhospitals.Inthegivenproblem,twoworkload-relatedobjectivesandfivegroupsofconstraintsareproposed.Abio-mimickedBinaryBeesAlgorithm(BBA)isintroducedtosolvethismultiobjectivemulticonstraintcombinatorialoptimisationproblem,inwhichconstrainthandlingtechnique(MultiobjectiveTransformation,MOT),multiobjectiveevaluationmethod(nondominanceselection),globalsearchstrategy(stochasticsearchinthevariablespace),localsearchstrategy(Hammingneighbourhoodexploitation),andpost-processingmeans(feasibilityselection)arethemainissues.TheBBAisthendemonstratedwithacasestudy,presentingtheexecutionprocessofthealgorithm,andalsoexplainingthechangeofelitenumberinevolutionaryprocess.Itsoptimisationresultprovidesagroupoffeasiblenondominatedtwo-leveldistributionschemes.

简介：Changesinthedistributionof1P1-antigeninthedevelopingchickretinahavebeenexaminedbyindriectimmunofluorescencestainingtechniqueusingthenovelmonoclonalantibody(MAb)1P1.Expressionofthe1P1antigenwasfoundtoberegulatedinradialaswellasintangentialdimensionoftheretina,beingpreferentiallyorexclusivelylocatedintheinnerandouterplexiformlayersoftheneuralretinadependingonthestagesofdevelopment,Withtheonsetoftheformationoftheinnerplexiformlayer1P1antigenbecomesexpressedintheretina.Withprogressingdifferentiationoftheinnerplexiformlayer1P1immunofluorescencerevealed2subbandsatE9and6subandsatE18,Atpostnatalstages(afterP3)immunoreactivitywasreducedinaninside-outsidesequenceleadingtothecompleteabsenceofthe1P1antigeninadulthood.1P1antigenexpressionintheouterplexiformlayerwasalsosubjecttodevelopmentalregulation.Thespation-temporalpatternof1P1antigenexpressionwascorrelatedwiththetimecourseofhistologicaldifferentationofchickretina,namelythesynapserichplexiformlayers.Whetherthe1P1antigenwasfunctionallyinvolvedindendriteextensionandsynapseformationwasdiscussed.

简介：Thelargeamountofrepeats,especiallyhighcopyrepeats,inthegenomesofhigheranimalsandplantsmakeswholegenomeassembly(WGA)quitedifficult.Inordertosolvethisproblem,wetriedtoidentifyrepeatsandmaskthempriortoassemblyevenatthestageofgenomesurvey.Itisknownthatrepeatsofdifferentcopynumberhavedifferentprobabilitiesofappearanceinshotgundata,sobasedonthisprinciple,weconstructedastatisticalmodelandinferredcriteriaformathematicallydefinedrepeats(MDRs)atdifferentshotguncoverages.Accordingtothesecriteria,wedevelopedsoftwareMDRmaskertoidentifyandmaskMDRsinshotgundata.Withrepeatsmaskedpriortoassembly,thespeedofassemblywasincreasedwithlowererrorprobability.Inaddition,clone-insertsizeaffectstheaccuracyofrepeatassemblyandscaffoldconstruction.Wealsodesignedlengthdistributionofclone-insertsusingourmodel.Inoursimulatedgenomesofhumanandrice,thelengthdistributionofrepeatsisdifferent,sotheiroptimallengthdistributionsofclone-insertswerenotthesame.Thuswithoptimallengthdistributionofclone-inserts,agivengenomecouldbeassembledbetteratlowercoverage.