简介：Expressedsequencetags(ESTs)arewidelyusedingenesurveyresearchtheseyears.TheESTPipelineSystem,softwaredevelopedbyHangzhouGenomicsInstitute(HGI),canautomaticallyanalyzedifferentscalarESTsequencesbysuitablemethods.Alltheanalysisreports,includingthoseofvectormasking,sequenceassembly,geneannotation,GeneOntologyclassification,andsomeotheranalyses,canbebrowsedandsearchedaswellasdownloadedintheExcelformatfromthewebinterface,savingresearcheffortsfromroutinedataprocessingforbiologicalrulesembeddedinthedata.

简介：Sincepigisanimportantlivestockspeciesworldwide,itsgeneexpressionhasbeeninvestigatedintensively,butrarelyinbrain.Inordertostudygeneexpressionprofilesinthepigcentralnervoussystem,wesequencedandanalyzed43,122highquality5′endexpressedsequencetags(ESTs)fromporcinecerebellum,cortexcerebrum,andbrainstemcDNAlibraries,involvingseveraldifferentprenatalandpostnataldevelopmentalstages.TheinitialESTswereassembledinto16,101clustersandcomparedtoproteinandnucleicaciddatabasesinGenBank.Ofthesesequences,30.6%clustersmatchedproteindatabasesandrepresentedfunctionknownsequences;75.1%hadsignificanthitstonucleicaciddatabasesandpartialrepresentedknownfunction;73.3%matchedknownporcineESTs;and21.5%hadnomatchestoanyknownsequencesinGenBank.WeusedthecategoriesdefinedbytheGeneOntologytosurveygeneexpressionintheporcinebrain.

简介：Seventy-fivepreviouslyknownplantmicroRNAs(miRNAs)wereclassifiedinto14familiesaccordingtotheirgenesequenceidentity.Atotalof18,694plantexpressedsequencetags(EST)werefoundintheGenBankESTdatabasesbycomparingallpreviouslyknownArabidopsismiRNAstoGenBank'splantESTdatabaseswithBLASTalgorithms.AfterremovingtheESTsequenceswithhighnumbers(morethan2)ofmismatchednucleotides,atotalof812ESTcontigswereidentified.AfterpredictingandscoringtheRNAsecondarystructureofthe812ESTsequencesusingmFoldsoftware,338newpotentialmiRNAswereidentifiedin60plantspecies,miRNAsarewidespread.SomemicroRNAsmayhighlyconserveintheplantkingdom,andtheymayhavethesameancestorinveryearlyevolution.ThereisnonucleotidesubstitutioninmostmiRNAsamongmanyplantspecies.SomeofthenewidentifiedpotentialmiRNAsmaybeinducedandregulatedbyenvironmentalbioticandabioticstresses.Somemaybepreferentiallyexpressedinspecifictissues,andareregulatedbydevelopmentalswitching.ThesefindingssuggestthatESTanalysisisagoodalternativestrategyforidentifyingnewmiRNAcandidates,theirtargets,andothergenes.AlargenumberofmiRNAsexistindifferentplantspeciesandplayimportantrolesinplantdevelopmentalswitchingandplantresponsestoenvironmentalabioticandbioticstressesaswellassignaltransduction.EnvironmentalstressesanddevelopmentalswitchingmaybethesignalsforsynthesisandregulationofmiRNAsinplants.AmodelformiRNAinductionandexpression,andgeneregulationbymiRNAishypothesized.

简介：表达序列标签是一种快捷、高效的揭示基因组信息的方法。本研究以蓝塘猪与大白猪差异表达的5条EsT在各组织中的表达情况为目的．采用大白猪、蓝塘猪为实验材料，从成年种猪的10个组织中提取总RNA；运用RT—PCR方法分析，结果显示5条EST在猪的多数组织中均有表达；同源性比对后发现，在5条差异显示cDNA中，EST1、EST2与已知序列无同源性；电子表达谱分析表明EST1、EST2、EST4及ESt5在骨骼肌、肝脏组织中有表达。这为进一步研究该EST的功能奠定基础。

简介：内窥镜下乳头括约肌切开术(Endoscpicduodenalpapilo-sphincterotomyEST)主要用于治疗胆总管结石及乳头括约肌狭窄[1],是胆总管结石介入治疗的重要方法之一;逆行性内窥镜胰胆管造影(EndoscopicretregradecholangiopancreatographyERCP)具有确定EST的适应证,指导网篮取石,发现合并症及观察疗效的作用,而成为EST的基础[2].我们自1996年11月～2003年2月共做EST320例,取得满意疗效.现将ERCP在其中的基础作用分析如下.

简介：ToobtainaprimaryoverviewofgenediversityandexpressionpatterninLycorislongituba,4,992ESTs(ExpressedSequenceTags)fromL.Longitubabudweresequencedand4,687cleanedESTswereusedforgeneexpressionanalysis.ClusteredbythePHRAPprogram,967contigsand1,343singletswereobtained.Blastsearchshowedthat179contigsand227singlets(totally1,066ESTs)hadhomologuesinGenBankand3,621ESTswerenovel.

简介：本课题组利用圭630/台湾粳的DH群体,建立了一个水稻RFLP连锁图.该图谱含175个标记,总长1224.6cM,相邻标记间平均距离为7.0cM.但该图标记分布不够均匀,存在较多空白区,且在第4和第8染色体上分别存在一个断点.本研究试图在原有RFLP图谱的基础上,添加一些SSR标记,以使该图谱标记更加密集和均匀,并消除两个断点.共筛选了361对RM引物,获得了183对多态标记,在12条染色体上共整合了57个RM标记,染色体连锁图总长度为1811.2cM,比原图谱增长了47.9%,相邻标记间平均距离为7.8cM,与原图谱相近.不过,两条染色体上的两个断点仍无法消除,暗示这两个断点区域多态性较低或重组率较高.

简介：英语和汉语虽属不同语系,但在主述位信息结构上存在共同点。语篇传递的信息,可以通过主述位信息结构分析来把握。本文就EST语篇中常见的几种主述位推进模式及其应用进行分析,以探讨主述位理论对EST语篇翻译实践的指导意义。

简介：ExpressedSequenceTag(EST)analysishaspioneeredgenome-widegenediscoveryandexpressionprofiling.Inordertoestablishageneexpressionindexinthericecultivarindica,wesequencedandanalyzed86,136ESTsfromninericecDNAlibrariesfromthesuperhybridcultivarLYP9anditsparentalcultivars.WeassembledtheseESTsinto13,232contigsandleave8,976singletons.Overall,7,497sequenceswerefoundsimilartotheexistingsequencesinGenBankand14,711arenovel.Thesesequencesareclassifiedbymolecularfunction,biologicalprocessandpathwaysaccordingtotheGeneOntology.WecomparedoursequencedESTswiththepubliclyavailable95,000ESTsfromjaponica,andfoundlittlesequencevariation,despitethelargedifferencebetweengenomesequences,Wethenassembledthecombined173,000riceESTsforfurtheranalysis.UsingthepooledESTs,wecomparedgeneexpressioninmetabolismpathwaybetweenriceandArabidopsisaccordingtoKEGG.Wefurtherprofiledgeneexpressionpatternsindifferenttissues,developmentalstages,andinaconditionalsterilemutant,aftercheckingthelibrariesarecomparablebymeansofsequencecoverage.Wealsoidentifiedsomepossiblelibraryspecificgenesandanumberofenzymesandtranscriptionfactorsthatcontributetoricedevelopment.

简介：目的：观察十二指肠切开术(EST)及乳头球囊扩张术(EPBD)在胆源性胰腺炎的治疗中有效性和安全性。方法：30例胆源性胰腺炎患者B超或CT提示均有胆总管高压或胆总管结石。其中胆总管结石20例(单纯胆总管结石12例，并胆囊结石6例，并肝内胆管结石2例；18例胆总管结石直径≤1．0，2例胆总管结石直径>1．0)，单纯胆囊结石8例，单纯胆总管扩张2例。所有患者于入院24h内均行EST，EPBD或行胆总管网篮取石术。术后观察临床症状，血生化，血常规及体温变化等生化指标。结果：28例于术后第3天腹痛消失，血淀粉酶于术后第7天基本降至正常。28例达到临床治愈，2例好转，2例并发切口处出血。治愈率高达93．3％，并发症出现率为6．7％。结论：EST及EPBD或网篮取石治疗胆源性胰腺炎治愈率高，简单安全，胆总管小结石(直径小于1．0cm)性胰腺炎患者免于胆总管探查T管引流，降低手术并发症及风险性，缩短住院时间：对部分肝内外胆管结石及胆囊结石性胰腺炎患者，可为手术创造良好条件。

简介：由使用能识别栽培米饭(OryzasativaL)的二亚种(indica和装饰用的梨树)的19份教材，从在Yuanjiang的普通野米饭(OryzarufipogonGriff)的自然人口的56个个人的indica装饰用的梨树区别被SSR分析(微卫星DNA，或简单顺序重复)。19份教材，17对(89.47%)能个人在所有之中放大一个种仅仅乐队类型，但是教材RM251andRM18能放大多态的乐队类型。16份教材(84.21%)放大的乐队与indica装饰用的梨树相同在栽培米饭的相关地点的诊断乐队，包括11像装饰用的梨树的loci和4象indica一样loci。乐队由另外的三份教材放大了(RM18，RM202，RM205)与indica或装饰用的梨树不同栽培米饭的诊断乐队。结果证明根据分析的19loci，在Yuanjiang的普通野米饭的SSRlociingenomic脱氧核糖核酸的84.21%显示了indica装饰用的梨树区别，13.79%loci仍然保持原始，并且大多数检测loci是在自然人口基因的人。

简介：本研究应用ISSR和SSR技术建立了24个水稻光温敏核不育系的DNA指纹图谱,利用13个ISSR引物和20对SSR引物,分别获得174个多态性片段和62个多态性片段,平均每个ISSR引物检测到13.38个多态性片段,远远高于SSR引物的检测率.根据遗传距离进行的聚类分析表明,利用这两种标记所得的聚类结果十分相似,24个材料被聚为粳型,偏籼型和籼型三个类群,在籼型不育系类群内,又可明显的分成三个亚类群,其中7个安农S-1衍生的不育系聚为一类,与农垦58S衍生的不育系有明显的遗传差异.根据两种标记计算的遗传距离及其遗传关系,所得的结果仍有一些差异,但总体趋势是一致.研究结果表明,ISSR和SSR标记适用于构建DNA指纹图谱,进行分类鉴定和遗传分析.