简介:Thewaxygene(Wx)inrice,whichencodesthegranuleboundstarchsynthaseenzyme,isresponsibleforamylosesynthesis.Glutinous(sticky)ricehaslittleornoamylosethatcanbeusedinvariousapplications,suchasbrewing.Inthisstudy,knockoutoftheWxgenewithCRISPR/Cas9technologywasconductedintwoelitejaponicaricelines,Huaidao5(HD5)andSuken118(SK118),aimingtodevelopelitestickyricevarieties.WeachievedsixhomozygousT0plantswithmorethan200bpdeletionintheWxgene,aswellas36wx-HD5and18wx-SK118homozygoustransgene-freeplantsintheT1generation.Theseedsofallthemutantswerewhiteandopaque,similartothoseofstickyrice,andcontainedonly2.6%–3.2%amylose.Resultsofscanningelectronmicroscopyshowedthatthequalityofricedidnotchange.Inconclusion,wesuccessfullydevelopedtwoelitestickyricevarieties.
简介:Afuzzyparticleswarmoptimization(PSO)onthebasisofelitearchivingisproposedforsolvingmulti-objectiveoptimizationproblems.First,anewperturbationoperatorisdesigned,andtheconceptsoffuzzyglobalbestandfuzzypersonalbestaregivenonbasisofthenewoperator.Afterthat,particleupdatingequationsarerevisedonthebasisofthetwonewconceptstodiscouragetheprematureconvergenceandenlargethepotentialsearchspace;second,theelitearchivingtechniqueisusedduringtheprocessofevolution,namely,theeliteparticlesareintroducedintotheswarm,whereastheinferiorparticlesaredeleted.Therefore,thequalityoftheswarmisensured.Finally,theconvergenceofthisswarmisproved.TheexperimentalresultsshowthatthenondominatedsolutionsfoundbytheproposedalgorithmareuniformlydistributedandwidelyspreadalongtheParetofront.
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简介:Background:Psychologicalstressandrecoverymonitoringisakeyissueforincreasingathletes’health,well-being,andperformance.Thismultistudyreportexaminedchangesandthedoseàresponserelationshipsbetweenrecoveryàstresspsychologicalstates,trainingload(TL),heartrate(HR),heartraterecovery(HRR),andheartratevariability(HRV)whileprovidingevidenceforthefactorialvalidityofashortFrenchversionoftheRecoveryàStressQuestionnaireforAthletes(RESTQ-36-R-Sport).Methods:Fourhundredandseventy-threeuniversityathletes(Study1),72fullexpertswimmers(Study2),and11nationaltointernationalswimmers(Study3)participatedinthestudy.Datawereanalyzedthroughconfirmatoryfactoranalyses(Study1),repeatedANOVAsandcorrelationalanalyses(Study2),ttestsandcorrelationalanalyses(Study3).Results:Multiple-groupconfirmatoryfactoranalysesshowedthattheRESTQ-36-R-Sportscoreswerepartiallyinvariantacrossgender,typeofsport,andpracticelevel(Study1).AdoseàresponserelationshipwasperformedbetweenTLandRESTQ-36-R-Sportscoresduringanecologicaltrainingprogram(Study2).Finally,relationshipswerefoundbetweenphysiological(HRR)andpsychological(RESTQ-36-R-Sport)statesduringanecologicaltaperingperiodleadingtoanationalchampionship(Study3).Conclusion:Asawhole,thesefindingsprovidedevidencefortheusefulnessoftheshortversionoftheRESTQ-36-R-Sportforregularmonitoringtopreventpotentialmaladaptationduetointensivecompetitivesportpractice.
简介:Introgressionlines(ILs)derivedfrominterspecificcrossesareasourceofnewgeneticvariability.Atotalof55ILsderivedfromtwocrossesSwarna×O.nivaraIRGC81848(populationA)andSwarna×O.nivaraIRGC81832(populationB)werecharacterizedforyieldandyield-relatedtraits/QTLs.Segregationof103simplesequencerepeat(SSR)markersassociatedwithyield-relatedQTLswasstudied.PopulationAshowedanaverageof12.6%homozygousO.nivaraallelesandpopulationBshowed10.6%.Interestingly,threeSSRmarkers,RM223,RM128andRM517,showedconspicuouspatternofsegregation.ThedistributionofparentalallelesatthreelociRM223,RM128andRM517linkedtoyield-relatedtraitswasunique.Thesemarkersflankedtoseveralyield-relatedQTLs.RM223,flankingtoqyld8.3,washeterozygousinalmostallthe55ILsexceptinIL10-3SandIL131S.RM128onchromosome1andRM517onchromosome3weremutuallyexclusivein46outof55ILs.These46ILsshowedeitherofthemarkeralleleRM128orRM517fromO.nivarabutnotboth.IL166ShadbothRM128andRM517fromO.nivaraandtheotherILsshowedhomozygousSwarnaalleleatRM517exceptIL65S.Populationstructureassignedthe55ILstothreesub-populationsbasedontheirgenomicdiversity.IL65S,IL166S,IL248S,IL7KandIL250Kshowedhighyieldsinmulti-locationtrials,andIL248SwasreleasedforcultivationasDRRDhan40.
简介:Toprovidegeneticinformationandmaterialsforbreedinghybridjaponicaricewithwideadaptabilityandstrongcompetitiveadvantageofyield,eliteallelesandtheircarriervarietiesofgrowthduration(GD)andproductivepaniclenumberperplant(PN)weredetected.Anaturalpopulationcomposedof94japonicavarietieswasphenotypedfortheGD,PNandplantheight(PH)intwoenvironments.TheconditionalphenotypicdataweretransferredbythelinearmodelmethodinsoftwareQGAStation1.0,andassociationmappingbasedontheunconditionalandconditionalphenotypevaluesofGDandPNwasanalyzedbyusinggenerallinearmodelinsoftwareTASSEL.Atotalof34simplesequencerepeat(SSR)markerlociassociatedwithGDandPNweredetectedinthetwoenvironments.Amongthem,15wereassociatedwithGD,and19wereassociatedwithPN.FoureliteallelesofRM8095-120bp,RM7102-176bp,RM72-170bpandRM72-178bpwereassociatedwithGD,andtheircarriervarietieswereHongmangshajing,Nipponbare,HongmangshajingandNannongjing62401,respectively.TheseeliteallelesfromthecarriervarietiescanshortenGDby2.039.93dwhentheywereintroducedintoimprovedmaterials.RM72-182bpassociatedwithPNwasaneliteallele,anditscarriervarietywasXiaoqingzhong.ItcanincreasePNbythreewhenintroducedintoimprovedmaterials.Moreover,theseeliteallelescanbeusedtoimprovetargettraitswithoutinfluencinganothertwotraits.
简介:Nearisogeniclinescarryinglarge-effectQTL(qtl12.1),whichhasaconsistentinfluenceongrainyieldunderuplanddroughtstressconditionsinawiderangeofenvironments,wereevaluatedunderwaterstressinthefields.Thelinewhichgavehigheryieldunderdroughtwascrossedwithalocaleliteline,PMK3,andforwardedtoF2:3generation.SignificantvariationwasfoundamongtheF2:3linesforagronomictraitsunderwaterstressinthefields.Lowtohighbroadsenseheritability(H)forinvestigatedtraitswasalsofound.Waterstressindicatorssuchasleafrollingandleafdryingwerenegativelycorrelatedwithplantheight,biomassandgrainyieldunderstress.Bulkedsegregantanalysis(BSA)wasperformedwiththemarkersinthevicinityofqtl12.1,andRM27933wasfoundtobesegregatedperfectlywellinindividualcomponentsofdroughtresistantanddroughtsusceptiblebulkswhichwerebulkedbasedonyieldunderwaterstressamongF2:3lines.Hence,thissimpleandbreederfriendlymarker,RM27933,maybeusefulasapotentiallyvaluablecandidatemarkerforthetransferoftheQTLqtl12.1intheregionalbreedingprogram.BioinformaticanalysisoftheDNAsequenceoftheqtl12.1regionwasalsodonetoidentifyandanalyzepositionalcandidategenesassociatedwiththisQTLandtoascertaintheputativemolecularbasisofqtl12.1.