学科分类
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299 个结果
  • 简介:自然数集按(mod6)排列后,将自然数符号螺旋对称分布的规律与自然律有机关联起来研究,从中发现数集中部分合数M中所包含相同素因子P,q周期分布与等距传递的规律,即双重素数P,q实项、虚项原构、同构同因子对应组螺旋对称分布结构的形式与规律,可模拟宇宙万物的时空螺旋运动状态,模拟DNA双螺旋结构碱基(A、T、G、C)序列遗传密码结构与形式。亦可作为一种创新方法开发应用研究,为研究DNA序列结构的数学编码,研究宏观宇宙、中观生物、微观质量的对应螺旋运动状态,预测宇宙万物相互关联相互作用的变化规律与趋势,构建基于宇宙中空时序的自然数双重素数因子对应组(多组)螺旋延伸的数码模型,高度抽象探讨与理解宇宙万物运动变化的原本规律,为相关问题的表述与解决提供数码螺旋的解决方案。

  • 标签: 螺旋结构 DNA遗传基因 合数应用 双重素数因子对 其他科学研究
  • 简介:DNA计算是近年来信息领域提出的一种全新的计算理念和模式,具有传统电子计算机不可比拟的优点。简要介绍了DNA计算的基本概念、特点、发展历程,4种基本的DNA计算模型及其应用,最后对DNA计算进行了展望。

  • 标签: DNA计算 粘贴系统 剪接系统 插入-删除系统
  • 简介:在AM1方法优化的几何结构基础上,用INDO/CI-SOS方法深入探讨硼羰基(B-C=O)螺旋共轭化合物的二阶非线性光学性质,讨论了硼羰基的不同取代位置对螺旋共轭体系的电子光谱、二阶非线性光学系数的影响.结果表明硼羰基的引入可改善体系的非线性光学性质,随着硼羰基个数的增加,二阶非线性光学系数增大,大入射波长为1064nm时,β绝对值比未被取代的螺旋共轭化合物的β值增大一个数量级,达到13.84×10-40C·m.

  • 标签: 共轭体系 二阶非线性光学系数 羰基取代 二阶非线性光学性质 电子结构 个数
  • 简介:ThebindingofCo(bpy)2dppz3+tocalfthymusDNAwasinvestigatedbyusingabsorptionandemissionspectroscopy,DNAmeltingtechniques,cyclicvoltammetry,viscosityandelectro-phoresismeasurements,wherebpyis2,2’-bipyridyl,dppzisdipyrido[3,2-o:2’,3’-c]phenazine.Thebindingcompoundshowsabsorptionhypochromicity,fluorescenceenhancement,andincreasingofDNAmeltingtemperatureandthespecificviscosity.CVmeasurementshowstheshiftsinoxidation-reductionpotentialandchangeinpeakcurrentwithadditionofDNA.ThecompoundisalsoshowntobemoreefficientphotosensitisersforstrandbreaksinplasmidDNA.

  • 标签: Cobalt (Ⅲ) COORDINATION compound CALF THYMUS
  • 简介:DNAcondensationisanimportantprocessinmanyfieldsincludinglifesciences,polymerphysics,andappliedtechnology.Inthenucleus,DNAiscondensedintochromosomes.Inpolymerphysics,DNAistreatedasasemi-flexiblemoleculeandapolyelectrolyte.Manyagents,includingmulti-valentcations,surfactants,andneutralpoorsolvents,cancauseDNAcondensation,alsoreferredtoascoil–globuletransition.Moreover,DNAcondensationhasbeenusedforextractionandgenedeliveryinappliedtechnology.ManyphysicaltheorieshavebeenpresentedtoelucidatethemechanismunderlyingDNAcondensation,includingthecounterioncorrelationtheory,theelectrostaticzippertheory,andthehydrationforcetheory.Recentlyseveralsingle-moleculestudieshavefocusedonDNAcondensation,sheddingnewlightonoldconcepts.Inthisdocument,themulti-fieldconceptsandtheoriesrelatedtoDNAcondensationareintroducedandclarifiedaswellastheadvancesandconsiderationsofsingle-moleculeDNAcondensationexperimentsareintroduced.

  • 标签: 脱氧核糖核酸 阳离子表面活性剂 场方法 高分子物理 缩合机理 DNA
  • 简介:TheeffectofCdionsonsalmonspermDNAwasstudiedbymeansofcirculardichroism(CD),Ramanspectroscopy,X-rayphotoelectronspectroscopy(XPS)andfluorescencespectroscopy.TheCDspectralandfluorescentprobe-acriflavineresultsindicatethattheDNAunderwentaconformationchangeupontheadditionofCdions.XPSandRamanstudiesrevealthatthereexistedinteractionsbetweenCdionsandthephosphategroupsoftheDNA.Inaddition,anewbandappearedat803cm-1intheRamanspectra,whichcanbeattributedtocharacterizing"marker"bandofA-DNA.ItisconcludedthatCdionscanbecoordinatedbythephosphategroupsoftheDNAandinducetheconformationchangesoftheDNAfromB-DNAtoA-DNA.

  • 标签: CD ion DNA RAMAN spectroscopy Conformation.
  • 简介:HydrolysisofDNAisanimportantenzymaticreaction,butitisexceedinglydifficulttomimicinthelaboratorybecauseofthestabilityofhydrolysisofDNA.Inthispaper,thecleavageactivityofcomplexesformedbetweenCu(Ⅱ)andfourdifferentaminoacidoraminoacidmethylesteronDNAisstudiedbygelelec-trophoresis.ItisfoundthatDNAcouldbecleavedbyCu(Ⅱ)-L-HisandCu(Ⅱ)-L-Hismethylestercomplexesandtheefficiencyofcleavageislargelydependentonthemetalion-to-ligandratio.FurtherexperimentsshowthatthecleavageofDNAmediatedbyCu(Ⅱ)-L-HiscomplexesoccursviaahydrolyticmechanismandtheactivechemicalspeciesthataffectsDNAcleavageisproposedtobeMI2H+andML2H22+.

  • 标签: Copper(Ⅱ) COMPLEXES DNA hydrolytic CLEAVAGE
  • 简介:针对数值计算中的乘法计算,通过编码特定的DNA分子,将所有计算中可能出现的DNA分子链设定为特殊结构DNA分子库,建立数值乘法DNA计算的自装配模型。相对于已有的针对数值计算的DNA计算模型,这种计算模型的优点是采用了并行计算的方式,特别在计算位数比较多时,表现出DNA计算极度并行的优点,使计算更加有效。虽然讨论的是十进制数的乘法,但其方法也适用于任意的N进制乘法运算。

  • 标签: DNA计算模型 数值乘法 并行计算 DNA分子链 乘法库
  • 简介:AdoublehelixmodelofchargetransportinDNAmoleculeisgivenandthetransmissionspectraoffourDNAsequencesareobtained.ThecalculatedresultsshowthatthetransmissioncharacteristicsofDNAarenotonlyrelatedtothelongitudinaltransportbutalsotothetransversetransportofmolecule.Theperiodicsequencewiththesamecompositionhasstrongerconductionability.Withtheincreasingofbasescomposition,theconductiveabilityreduces,buttheweightofθdirectionrisesinchargetransfer.

  • 标签: 电荷运输 DNA分子 传输工具 物理
  • 简介:Adetectionofanthracyclineantitumordrugdaunomycin(DNR)reactingwithDNAinsimulatemetabolisminvitrohasbeenmade.ItwasfoundthatDNRcouldreactwithDNAtoformDNR-DNAadducts.TheadductcompositionsofDNRwithfishspermDNAandthermallydenaturatedDNAweredetermined.TheequilibriumassociationconstantKofDNRwithfishspermDNAis1.98×10^5L/molandthatofDNRwithdenaturatedDNAis2.29×10^4L/mol.Semiquinonefreeradicals,metabolicproductsofDNR,candestroybothfishspermDNAanditsthermallydenaturatedDNA.ItisverifiedbyhyperchromiceffectincreaseobservedinUVspectrumandAFMexperiments.ThemechanismofDNAdegradationhasalsobeeninvestigated.Resultsobtainedallowonetoexplainthereasonofsideeffectofanthracyclinedrugandgivethewaytodepress,whichwereofclinicalsignificance.

  • 标签: 抗癌药物 道诺霉素 DNA 新陈代谢物 光谱化学分析
  • 简介:AvohammetricstudyoftheinteractionofneutralRed(NR)withDNAatagoldelectrodeinaphosphatebuffersolutionisdescribed.AfteraddingDNAinanNRsolution,thereductionpeakcurrentofNRdecreases.ThebindingmeehahismsofNRtoDNAindifferentpHrangesaredifferent.ThereductionpeakpotentialofNRinapH7.0phosphatebuffersolutioninthepresenceofDNAshiftspositively,indicatingthatthebindingofNRtoDNAisintercalationaction,butatpH=6.0thereductionpeakpotentialofNRshiftsnegatively,indicatingthatthebindingofNRtoDNAiselectrostaticaction.TheformedcomplexesareDNA-NRwhen[NR]/[DNA]<0.18andDNA-3NRwhen[NR]/[DNA]>0.35,respectively.

  • 标签: 中性红 DNA 循环伏安法 相互作用 核酸 信息复制
  • 简介:Moleculardynamics(MD)simulationsareperformedtostudyadhesionandpeelingofashortfragmentofsinglestrandDNA(ssDNA)moleculefromagraphitesurface.Thecriticalpeel-offforceisfoundtodependonboththepeelingangleandtheelasticityofssDNA.FortheshortssDNAstrandunderinvestigation,weshowthatthesimulationresultscanbeexplainedbyacontinuummodelofanadhesiveelasticbandonsubstrate.Theanalysissuggeststhatitisoftenthepeakvalue,ratherthanthemeanvalue,ofadhesionenergywhichdeterminesthepeelingofananoscalematerial.

  • 标签: 分子动态模拟 DNA 培养基 表面支持 弹性模型
  • 简介:MtDNAwassuccessfullyextractedfromtenindividualbones(femurs)inthetombsofancientJushiinTurfanbasin,datedbacktotheyearabout3000-2500yearsago.Bymeansoffouroverlappingprimers,wegotnucleotidesequenceofthe218bplength.AncientmtDNAwasanalyzedbythesequencingofhypervariableregionⅠofthemtDNAcontrolregion.Theresultshowsthat9haplotypeswith24polymorphicsiteswereobtained.ThephylogeneticanalysisindicatedthatMongoliansandAltaiarethepopulationgeneticallyclosesttotheJushigroupsandJushimtDNApoolbeinganadmixtureofeasternAsianandEuropeanlineages.SoourpreliminarydataimplythatanancientminglingofEuro-AsianpopulationhadexistedinTurfanbasinpriortotheearlyIronAge.

  • 标签: ANCIENT DNA Sequence PHYLOGENETIC ANALYSIS
  • 简介:Rheumatoidfactors(RFs)arethecharacteristicautoantibodiesofrheumatoidarthritis.Recentresearchesinourlaboratoryshowedthattheimmobilizedsingle-strandedDNA(ss-DNA)immunoadsorbentcanselectivelyremoveRFsfromtheserumofpatients.Inthepresentpaperarestudiedthemodificationofargininine,tryptophan,lysineresiduesandcarboxylterminusofIgGRF,whichwasseparatedfrompatients′serum,with1,2-cyclohexanedione(CHD),N-bromosuccinimide(NBS),pyridoxal5′-phosphate(PP)and1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC)respectively,andtheireffectsontheadsorptioncapacityoftheimmobilizedss-DNAimmunoadsorbentforIgGRF.Afterthespecificmodification,thecorrespondingadsorptioncapacitiesoftheadsorbentswerechangedfrom48%,46%,44%and54%to84%,14%,21%and81%,respectively.Theseresultsindicatethattheelectrostaticorionic-bondingisessentialfortheinteractionbetweenss-DNAandIgGRF.

  • 标签: 交互作用 风湿症 固定单链DNA 免疫吸附 1 2-环己二酮 N-四溴酚酞磺酸钠
  • 简介:TheroleplayedbyDNAinmolecularbiologyisclearlyrecognizedtobevitaltolifeonthisplanet.8-oxo-7,8-dihydro-2deoxyguanosine(=8-OHdG),isprobablythemostimportantproductof"oxidativestress”inDNA.ItsconcentrationinDNAis,infact.aquantitativeanalysisofthedegreeofDNAdamagethatanorganismhasundergone.Duetotheimportanceof8-OHdGofnucleicacidginmutagenesis,carcinogenesisandaging,numerouschemicalandbiologicalinvestigationshavebeenmadeonthissubjectinthepasttime.Kuchinoandco-workershavefoundthat8-OHdGresidueinDNAismisreadingduringtheprocessofDNAreplication.Recently,somereportshavebeenpresentedonhigh8-OHdGlevelsinpatientssufferingfromvariousdiseasessuchaschronichepatitis,Fanconisanemia,diabetesmellitusandHelicobacterpyloriinfections.Asaresult,8-OHdGisausefulmarkerforthestudyofDNAdamagearisingfromreactiveoxygenspeciesandisofgreatsignificanceforcancerresearch.The8-OHdGlevelsinDNAcanhelpunderstandthemechanismofcarcinogensandleadtomoreeffectivetreatmentsformanydifferenttypesofcancer.Forthesereasons,ananalysisof8-OHdGwithquickness,lowcostandhighaccuracyisrequired.

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  • 简介:ThisstudyintendstoinvestigatehowtheelasticityofabacterialphagecanafecttheprocessofDNApackagingandejection.Forthispurpose,weproposeaunifiedcontinuumandstatisticalmechanicsmodelbytakingintoaccounttheefectsofDNAbendingdeformation,electrostaticrepulsionbetweenDNA–DNAstrandsandelasticdeformationofthephagecapsid.Basedonsuchamodel,wederivethequantitativerelationsbetweenpackagingforce,elasticityofcapsid,DNAlengthremaininginthecapsid,osmoticpressureandejectiontime.Thetheoreticallypredictedresultsarefoundtoagreeverywellwithinvitroexperimentalobservationsintheliterature.

  • 标签: 统计力学模型 DNA链 弹性变形 噬菌体 包装 弹射
  • 简介:在文献中,DNA序列曾被描述为一维游动和三维游动.对前者,一个游动对应于多个DNA序列;对后者,游动和DNA序列一一对应.我们发现在三维游动(xn,yn,zn)中,由xn,yn和zn中任意有序的两个给出的二维游动已经与DNA序列一一对应,且余下的一维游动由该二维游动完全决定.因此,二维游动似乎是描述DNA序列最合适的模型.4个碱基A,C,G和T共有4!=24个排序.每一个排序都给出DNA序列用二维游动的一种描述.两个游动(x'n,y'n)和(x"n,y"n)被看作是等价的,如果(x'n,y'n)=(εx"n,δy"n)或(εy"n,δx"n),这里ε=±1,且δ=±1.于是这24个类型的游动被分成三个等价类;它们的代表分别是(xn,yn),(yn,zn),和(xn,zn),这里(xn,yn,zn)正好是张和张的三维游动.

  • 标签: DNA序列 二维游动 一一对应 模型