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简介:摘要目的探讨血清中肿瘤标志物CEA、CA199、CA242、CA72.4、CA125在晚期胃癌中的临床应用价值。方法采用生化或放免分析法检测晚期胃癌患者血清CEA、CA199、CA242、CA72.4、CA125,与对照组比较并分析结果。结果32例胃癌患者及40例为胃良性患者及40例正常人CEA、CA199、CA242、CA72.4、CA125的敏感性依次为9.7.0%、5.6%、3.2%、6.2%、3.2%;晚期胃癌患者阳性率依次为37.5%、40.1%、28.1%、59.3%、18.7%。CA72.4的敏感性和特异性最高。结论CEA、CA199、CA242、CA72.4、CA125是晚期胃癌患者辅助诊断有价值的指标。
简介:AbstractBackground:Investigations of the pathogenic mechanisms in motor neurons (MNs) derived from amyotrophic lateral sclerosis (ALS) disease-specific induced pluripotent stem (iPS) cell lines could improve understanding of the issues affecting MNs. Therefore, in this study we explored mutant superoxide dismutase 1 (SOD1) protein expression in MNs derived from the iPS cell lines of ALS patients carrying different SOD1 mutations.Methods:We generated induced pluripotent stem cell (iPSC) lines from two familial ALS (FALS) patients with SOD1-V14M and SOD1-C111Y mutations, and then differentiated them into MNs. We investigated levels of the SOD1 protein in iPSCs and MNs, the intracellular Ca2+ levels in MNs, and the lactate dehydrogenase (LDH) activity in the process of differentiation into the MNs derived from the controls and ALS patients’ iPSCs.Results:The iPSCs from the two FALS patients were capable of differentiation into MNs carrying different SOD1 mutations and differentially expressed MN markers. We detected high SOD1 protein expression and high intracellular calcium levels in both the MN and iPSCs that were derived from the two SOD1 mutant patients. However, at no time did we observe stronger LDH activity in the patient lines compared with the control lines.Conclusions:MNs derived from patient-specific iPSC lines can recapitulate key aspects of ALS pathogenesis, providing a cell-based disease model to further elucidate disease pathogenesis and explore gene repair coupled with cell-replacement therapy. Incremental mutant expressions of SOD1 in MNs may have disrupted MN function, either causing or contributing to the intracellular calcium disturbances, which could lead to the occurrence and development of the disease.
简介:目的探讨胸水中CEA、CA125、CA153及CA199在肺癌诊断中的价值与意义。方法采用电化学发光免疫分析法测定40例肺癌患者与40例良性疾病患者胸水中的CEA、CA125、CA153及CA199水平,并对测定值进行组间比较;运用Logistic回归分析筛选出敏感指标,并进行联合检测。结果肺癌组患者胸水中的CEA、CA125、CA153及CA199水平分别为(43.28±8.19)ng/mL、(39.72±8.18)U/mL、(157.80±19.25)U/mL及(84.29±7.31)U/mL;良性疾病组四项标志物含量水平分别为(1.64±0.26)ng/mL、(26.47±4.26)U/mL、(12.31±3.86)U/mL及(31.47±6.10)U/mL,组间比较结果显示,肺癌组均高于良性疾病组中(P〈0.05)。以CEA、CA125、CA153及CA199为协变量,病理诊断结果作为应变量,CEA、CA125与CA153进入回归方程(P〈0.05)。肺癌组CEA、CA125、CA153单项检测阳性率分别为40.0%、52.5%与55.0%,三项联检阳性率为77.5%,显著高于各单项检测阳性率(P〈0.05)。结论胸水中CEA、CA125、CA153的检测在肺癌的诊断中具有重要意义,但是由于单项检测敏感性较低,可通过三项联合检测来克服单项指标的不足,提高肺癌诊断的敏感度及准确度。
简介:摘要目的探讨联合检测血清癌胚抗原(CEA)、抗原125(CA125)、癌抗原199(CA199)、癌抗原153(CA153)对早期辅助诊断肺癌的价值。方法采用全自动化学发光免疫分析法,检测51例肺癌(肺癌组),65例非良性疾病(对照组)血清CEA、CA125、CA153及CA199水平。结果肺癌组CEA、CA125、CA199、CA153血清水平明显高于对照组(P<0.01),单项肿瘤指标CEA、CA125、CA199、CA153的检测灵敏度分别为56.86%、50.98%、64.71%、60.78%,联合检测的灵敏度达92.16。结论血清肿瘤标志CEA、CA125、CA199、CA153的联检可提高早期辅助诊断肺癌的准确率,可为肺癌的早期诊断提供有价值的资料。
简介:摘要目的探讨血清糖类抗原125(CA125)、糖类抗原199(CA199)、糖类抗原724(CA724)、糖类抗原153(CA153)联合检测在卵巢癌诊断中的应用价值。方法抽取2018年3月至2021年3月信阳市中心医院收治的卵巢癌患者106例作为卵巢癌组,以1∶1配比抽取同期卵巢良性肿瘤106例作为良性肿瘤组,并以1∶1抽取同期健康体检者106例作为健康对照组。三组均检测血清肿瘤标志物(CA125、CA199、CA724、CA153)水平,比较三组间及卵巢癌组中不同国际妇产科协会(FIGO)分期、有无淋巴结转移患者的血清CA125、CA199、CA724、CA153水平,统计阳性检出率,分析相关性。结果卵巢癌组血清CA125、CA199、CA724、CA153水平均高于良性肿瘤组和健康对照组(P均<0.05)。卵巢癌组中,Ⅳ期和淋巴结转移患者血清CA125、CA199、CA724、CA153水平高于Ⅰ期、Ⅱ期、Ⅲ期患者和无淋巴结转移者(P<0.05)。CA125、CA199、CA724、CA153联合诊断检出率均高于单独诊断(P<0.05)。Pearson分析结果显示,CA125(r=0.647、0.639)、CA199(r=0.671、0.691)、CA724(r=0.704、0.712)、CA153(r=0.628、0.659)和FIGO分期、淋巴结转移均呈正相关(P均<0.05)。结论卵巢癌患者CA125、CA199、CA724、CA153表达水平较高,联合诊断可提高阳性检出率,并可用于评估FIGO分期和有无淋巴结转移,利于指导临床诊治。
简介:目的检测2016年8-11月和2017年7-10月在襄阳职业技术学院附属医院进行健康体检的40岁以上女性肿瘤标记物(TM)糖类抗原125(CA125)、糖类抗原153(CA153)和糖类抗原199(CA199),对这三种肿瘤标记物检测结果进行分析,探讨这三种肿瘤标记物对中老年女性健康体检的意义。方法应用化学发光方法对1722名中老年女性进行CA125、CA153和CA199检测,并对检测结果进行统计分析。结果三种TM共检出阳性73人次,其中CA125检出40人次,检出百分比为7%明显高于其它两种TM。女性年龄60岁以上的检出59人次,占比为81%明显高于60岁以下女性,其中CA199阳性人群中60岁以上的检出15人次,检出率高达93%,明显高于其它两种TM。结论CA125、CA153和CA199这三项肿瘤标记物对女性常见肿瘤的筛选和排除有一定的诊断意义,在中老年女性的健康体检中要重视和加强对TM的检测和监测,尤其是年龄较大(≥60岁)的老年女性更应该定期检测和监测,利于早期发现肿瘤,发现早期肿瘤,减少漏检。