简介：摘要目的探讨自体骨髓间充质干细胞(BMSCs)复合聚乳酸共聚物(PLGA)多孔可吸收支架修复猪退变纤维环的效果。方法选择贵州小型猪4头，每头猪选择腰2、3，腰3、4，腰4、5及腰5骶1椎间盘共16个椎间盘，平分为实验组及对照组，各8个椎间盘。实验组将BMSCs复合PLGA多孔可吸收支架植入猪纤维环退变破裂模型处，缝合后纵韧带避免植入体滑脱，对照组直接修复后纵韧带，在术后24周与48周记录修复效果。结果所有猪建模后都成活，两组术后24周的MRI椎间盘高度对比差异无统计学意义(P>0.05)，两组术后48周的MRI椎间盘高度都高于术后24周(P<0.05)，实验组高于对照组(P<0.05)。术后24周两组都不能分辨髓核组织，纤维向内塌陷，纤维环板层出现显著断裂；术后48周实验组椎间盘髓核组织为透明胶冻状，椎间隙清晰，纤维环及髓核的界限清楚。结论自体BMSCs复合PLGA多孔可吸收支架修复猪退变纤维环能促进提高椎间盘高度，安全性高，可改善椎间盘病理学状况。

简介：Three-dimensional-printed(3D-P)titaniumimplantsdisplaymanyadvantages,suchasdesignflexibility,higherefficiency,thecapabilitytoeasilyconstructcomplexorcustomizedstructures,etc.,andisbelievedtopotentiallyreplacetraditionalimplants.However,thebiologicalperformanceofthe3D-Ptitaniumsurfacehasnotbeeninvestigatedsystematically.Herein,weanalyzedthesurfacecharacteristicsof3D-PTi6AI4Vimplantsandevaluatedthebiologicalresponsesofbonemarrowderivedmesenchymalstromalcells(BMSCs)tothe3D-Psurfaceinvitro.Moreover,afterimplantationintotheratfemoralcondylefor3and6weeks,theosseointegrationperformaneewasevaluated.Theresultsshowedthe3D-PTi6Al4Vimplantpresenteddistinctfluctuantmacroscaleroughsurfaceandrelativelybetterhydrophilicitywhichenhancedtheadhesion,proliferation,osteogenicdifferentiationandangiogeneticfactorexpressionofBMSCs.Moreover,theinvivoosseointegrationperformancewasalsobetterthanthatofthecontrolgroupattheearlystage.Thepresentstudysuggestedthe3D-Ptitaniumalloyisapromisingcandidatetobeusedasimplantmaterial.