简介：Apicalmembranerecyclinghasbeenproposedtobeimportantfornormalhaircellfunction.Thecurrentstudyreportsaninvitroworkthatdemonstratesthepresenceofphosphatidylserine(PS)andPS-positivevesicleslabeledbyAnnexinVintheapicalportionofhaircells.ThefollowingcharacteristicsofthePS-positivevesicleswerenoticedusingscanningconfocalfluorescencemicroscopy:(1)variablesizesaround200nm;(2)variabledistributionpatterns(eitheruniformlyalongindividualstereociliainthehairbundleorirregular)inthestereociliafromcelltocell;(3)variablesizesandnumbersatlocationsalongtheborderofthecuticularplate(CP),withalargenumberofthemlocatedatthevestigalkinociliallocation;(4)motilitywithsomeofthevesiclesduringtheobservationperiod;(5)increaseinPSlabelingandthenumberofPS-positivevesiclesafterloudsoundstimulation;and(6)decreasedPSlabelingandPS-positivevesiclenumbersfollowingtreatmentwithLY-294002,aPI3-kinaseinhibitor.TheseresultssuggestthatthepresenceofPS-positivevesiclesattheapicalareaofhaircellsmaybeindicativeofvesiclesheddingortransportationofaproteinorrafts.

简介：客观：调查长期的权利的影响在室的改变和病人的心脏的功能与上的室的顶端的踱步高级并且酷刑逼供有正常的心结构和心脏的功能的心房与心室的阻塞。另外，我们为选最佳的电极培植为site.Methods提供许多证据：学习参加者包括了为心律调整器代替被招收并且在门诊病人为植入的心律调整器的考试重游的病人。心律调整器被植入到高级的对待和酷刑逼供心房与心室的阻塞。在心律调整器培植的时候，病人们有正常心脏的功能并且没显示出严肃的心疾病或心脏的膨胀。到后续的从培植的持续时间是超过5年。踱步的率比80%高。有左室的喷射部分(LVEF)的病人<50%并且一条左室的结束心脏舒张的直径(LVEDD)>55公里被排除。室的改变被定义为follows:increase在10%的LVEDD和为在培植以后的五年的在25%的LVEF的减小。心脏的功能根据纽约心协会(NYHA)classification.Results被评估:有吝啬的年龄的82个病人的一个总数(66.97?????????????????吗？？

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简介：目的：通过体外及体内实验探讨大鼠切牙Apicalbud上皮细胞诱导大鼠脂肪来源间充质干细胞向成牙本质细胞分化的能力。方法：分离培养大鼠脂肪间充质干细胞、Apicalbud上皮细胞,制备Apicalbud上皮条件诱导液并体外对ADSCs诱导7d,通过实时定量PCR方法检测成牙本质关键基因DMP-1及DSPP的表达。制备ADSCs及Apicalbud上皮重组细胞团进行大鼠肾被膜下移植,8W后取材,分别采用HE染色、Masson三色法和免疫组化方法对移植生成物进行组织学检测。结果：Apicalbud上皮诱导ADSCs后DMP-1及DSPPmRNA表达水平显著高于对照组。体内移植8W后HE染色可见管样牙本质和骨样牙本质样结构,Masson三色法可以观察到有绿色的牙本质样结构,免疫组化检测中CK14染色阳性,DSPP染色阳性。结论：Apicalbud上皮细胞在体外能够诱导ADSCs向成牙本质细胞分化,且细胞重组在体内可以构建出牙本质样结构。

简介：Plasmamembrane(PM)Ca^2+-ATPaseactivityinpoplarapicalbudmeristematiccellsduringshort-day(SD)-induceddormancydevelopmentwasexaminedbyaceriumprecipitationEM-cytochemicalmethod.Ca^2+-ATPaseactivity,indicatedbythestatusofceriumphosphateprecipitatedgrains,waslocalizedmainlyontheinteriorface(cytoplasmicside)ofthePMwhenplantsweregrownunderlongdaysandreachedadeepdormancy.Afewreactionproductswerealsoobservedonthenuclearenvelope.Whenplantbudsweredevelopingdormancyafter28to42dofSDexposure,almostnoreactionproductswerepresentontheinteriorfaceofthePM.Incontrast,alargenumberofceriumphosphateprecipitatedgrainsweredistributedontheexteriorfaceofthePM.After70dofSDexposure,whenbudshaddevelopedadeepdormancy,thereactionproductsofCa^2+-ATPaseactivityagainappearedontheinteriorfaceofthePM.TheresultsseemedsuggestingthattwokindsofCa^2+-ATPasesmaybepresentonthePMduringtheSD-induceddormancyinpoplar.OneistheCa^2+-pumpingATPase,whichislocatedontheinteriorfaceofthePM,formaintainingandrestoringtheCa^2+homeostasis.Theothermightbeandecto-Ca^2+-ATPase,whichislocatedontheexteriorfaceofthePM,fortheexocytosisofcellwallmaterialsassuggestedbythefactofthecellwallthickeningduringthedormancydevelopmentinpoplar.