简介:Thenewly-hatchednymphsofthesmallbrownplanthopper(SBPH),Laodelphaxstriatellus,includingfieldandsensitivepopulations,weresubjectedtothehigh-temperature(35°C)treatment.Thenumberofyeast-likeendosymbiotesinSBPHreducedby23.47%-34.23%,57.86%-61.51%and88.96%-90.71%afterthehigh-temperaturetreatmentfor1d,2d,and3d,respectively.However,thesizeofyeast-likeendosymbioteswasnotobviouslyaffected.ResistanceofSBPHtothreeinsecticides(imidacloprid,chlorpyrifosandfipronil)decreasedwiththeincreaseoftreatmenttime.
简介:MADS-box基因家族中的AG(AGAMOUS)基因对心皮和胚珠的发育起重要作用。本研究以甜樱桃(PrunusaviumL.)为试验材料,通过RT-PCR扩增AG保守片段,利用RACE技术获得一个全长表达序列,采用实时荧光定量的方法研究了外源赤霉素对该基因表达的影响。结果表明:(1)所获得基因PaMADS5(Genebank登录号:JQ686726),全长为1092bp,编码246个氨基酸;(2)蛋白同源性分析和系统进化分析表明,PaMADS5属于MADS-box家族中的AG亚族;组织特异性分析表明,PaMADS5在花的雄蕊和心皮中表达。(3)PaMADS5在雌蕊中的表达量受赤霉素的影响,随着赤霉素处理浓度的升高,PaMADS5的表达逐渐升高,表明PaMADS5可能参与调控甜樱桃雌蕊的发育。
简介:Theproteinstructureofthecellulosesynthase-likeprotein(CSL)wassimilartocellulosesynthase(CesA),includingtheconservativesequenceD,D,D,QXXRW.Onefull-lengthcDNAofthecellulosesynthase-likeproteinD(CslD)genewasclonedbyreversetranscriptase(RT)-polymerasechainreaction(PCR)with5’,3’rapidamplificationofcDNAends(RACE)methodsusingdegenerateprimersdesignedfromthehomologoussequencesoftheCesAgenes.Amultiplecomparisonsequenceanalysiswasconductedconcurrentlywithbioinformaticmethodstoanalyzetheobtainedsequence.ResultsofthesequenceanalysisshowedthatthiscDNAwas4150bpinlengthandcontainedasingleopenreadingframeencodingaproteinof1132aminoacids.Themultiplecomparisonsequenceanalysisshowedthatthededucedaminoacidsequencesharedhighsimilarity(over71%)withtheClCslDgenesfromPopulustremuloides,Oryzasativa,andArabidopsisthaliana.Thisworkwillhelplayanimportantfoundationforfurthermolecularstudieswithcellulosesynthesisofplants.