简介：Amethodforrapiddeterminationofsiliconcontentinricewasintroduced.Thereliabilityofthismethodwasverifiedbyusingarecombinantinbredline(RIL)populationofricecrossZhenshan97B/Milyang46.Twohundredandforty-nineRILsweretransplantedintworeplications.Simplecorrelationcoefficientsonthesiliconcontentinthehull,flagleafandsteminricebetweenduplicatesamplesof498ricematerialswere0.97954,0.97026and0.98848,respectively.Tenrepresentativesampleswereselectedformeasurementusingthehigh-temperaturealkalinefusionmethod.Simplecorrelationcoefficientbetweenthesiliconcontentsdeterminedbythehigh-temperaturealkalinefusionmethodandbythepresentmethodis0.9993.

简介：十四个米饭变化的长叶的率在营养生长阶段在一个播种时间实验，和快速的长叶遗传型被测量在indica变化Yanhui559被发现。Yanhui559的主要灰煤杆上的叶数字总是是多于Lemont的4-5叶子，并且Yanhui559的长叶的率基于从播种到出发的类似的生长持续时间比Lemont的显著地高。而且，长叶的率的差别在营养生长阶段是重要的，然而并非在圆锥花序开始阶段特殊。在Yanhui559和Lemont的二张回交人口的长叶的率的Gerietic分析证明那个学生和数量基因控制了快速的长叶特性的表示。每植物在backcrossing人口，更早展出到ering为止的快速的长叶遗传型和更多的tiller与类似的生长持续时间基于一些植物的调查的结果，并且它然后每圆锥花序每谷物的植物和数字包括圆锥花序的棕土的产量构成因素比慢长叶遗传型的那些优异。在繁殖的快速的长叶遗传型的进一步的研究和申请可行性被讨论。

简介：AnefficientandgoodDNAextractionprotocolshouldbesimple,affordableandyieldenoughDNAwithhighquality.Rice(OryzasativaL.)DNAextractionmethodsoftenuseseedlingsorleavesratherthanthegrainsandtendtobetime-consuming,involvemultiplesteps,andusehazardouschemicalsandexpensiveenzymes.RicegrainsofferseveralbenefitsoverseedlingsandleavesasasourceofDNAforgeneticanalysis.However,thesebenefitsareunderutilizedbecausethebulkofaricegrainismadeupofstarch.Itisparticularlyimportant,butdifficulttogetridofthestarchwhileextractingDNAfromricegrains.Thisco-precipitatedpolysaccharideisaknowninhibitorofDNApolymeraseactivityinpolymerasechainreaction(PCR).WedescribehereaverysimpleandhighlyaffordableChelex?-100basedDNAextractionmethodfromricegrains.Itdoesnotrequireanyhazardouschemicalsorenzymes.ThismethodreproduciblyextractsDNAwithgoodpurityindices(A260/A230andA260/A280values),butrequiresonlyafewsteps.

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简介：粘贴的性质在淀粉的最重要的特征之中，决定它在食物处理和另外的工业的应用。粘贴温度源于快速的Visco分析器(RVA)(Newport科学)在大多数情况中，被Thermocline为Windows过高估计软件节目。这里，便于由RVA粘贴温度的精确测量的二个方法被描述。一个人到变化参数设定到“屏幕”粘贴的粘性开始增加的真点，其它将手工地记录粘贴的粘性开始与公式增加并且计算粘贴的温度的时间(T1)(45/3.8)为米饭的x(T1-1)+50面粉。后者方法给了显著地与gelatinization被相关的手工地坚定的粘贴温度微分扫描caiorimetry测量的温度。

简介：RiceisanimportantfoodcropinChina,andthedevelopmentofhybridriceisacrucialwaytoincreasegrainyield.Thecreationofdual-purposenuclear-sterilelinesfortwo-linehybridbreedinghasbecomevitalforcommercialricebreeding.WeconstructedthepC1300-2x35S::Cas9-sgRNAPTGMS2-1expressionvectorforeditingthemalefertilitygenePTGMS2-1intwowidelycompatiblericevarieties,93-11andHuazhan,byusingtheCRISPR/Cas9system.Weobtainedthemarker-freephotoperiod-/thermo-sensitivegenicmale-sterile(P/TGMS)linesinT1generation.Accordingtotheexperimentsinphytotronwithfourtemperatureandphotoperiodtreatments,wefoundthetemperatureisthemainfactorforrestoringthepollenfertilityofptgms2-1mutantsin93-11andHuazhan,andthephotoperiodalsohassomeeffectsonpollenfertilityintwodifferentricebackgrounds.Theapplicationofcultivatingnewmale-sterilelinesbygenomeeditingsystemwillsignificantlyacceleratethericebreedingprocess.