简介：Objective:Toinvestigatetheapplicationofpolymerasechainreaction(PCR)detectionofHaemophilusducreyiinclinicaldiagnosisofchancroid.Methods:Nucleotidesequencesof16srRNAgenespecificforH.dureyiwereusedtodevelopprimersetsforamplificationoftwostrains.TheamplifiedproductsweretestedviaPCRandsequencedbyelectrophoresisina1.5%gel.TheseproductswerecomparedwiththoseofheterogeneousspeciesorrelatedbacteriatotestthespecificityofthePCRassay.PCRamplificationwithdifferentconcentrationsofH.ducreyiwasperformedtotestitssensitivity.Results:PCRamplificationoftwostrainsofH.ducreyiproducedasinglebandofexpected438bplength.ThesequencewasidentifiedwithgenomicDNA.Noneoftheother19referencespeciesamplifiedunderthesameconditionsgavethisresult.ThehighestsensitivityofPCRassayinthepresenttestwas10ng/L.Conclusions:PCRassayfordetectionofH.ducreyiisarapid,specific,andsensitivedetectionmethod.Iflaboratoryconditionsarestrictlycontrolled,PCRassayisapotentiallyusefullaboratorytestforH.ducreyiinfectiondiagnosis.

简介：Objective:Tofindoutthebasicconditionsofmycosisinfectionoffull-termpregnantwomen.Methods:Fungusisolationandidentificationfromthevaginalsecretionsandoralspecimensfrom208full-termpregnantwomenwerecarriedoutbyfungicultureandidentifiedfollowingstandardbiologicalpractice.Results:Thepositivedetectionrateforvaginalfungiwas51.92%andfororalfungiitwas47.59%.20.19%hadfungiinboththevaginalandoralcavity.ThefungifoundweremainlyofthespeciesCandidaalbicans.Conclusion:Theprevalenceoffungalmycosisinfectioninfull-termpregnantwomenwashigherthanthatusuallyfoundinnon-pregnantwomen.Inordertopreventfurthercomplicationsandmateral-childtransmission,seriousattentionshouldbepaidtothishighprevalencefinding.

简介：Objectives:TocharacterizethedistributionpatternofbiovarsandserotypesofUreaplasmaurealyticuminnormalhealthywomen,sexuallytransmittedinfectionsclinicclientsandinsexworkers.Methods:Weculturedcervicalswabstakenfrom261physicalcheck-upclients,599STIclinicoutpatientsand98sexworkersusingcommercialselectivemedium.SomepositivecultureswerefurtherbiotypedandserotypedbyPCR.Results:(1)Biovar1ofU.urealyticum(95.0%),especiallysingleinfectionofserotype1,3,and6ofbiovar1,iscommonlyfoundinhealthywomen.(2)Uourealyticumismorecommonlyisolatedinsexworkers(90.8%)thaninphysicalcheck-upgroup(60.9%)andSTIoutpatientsgroup(61.3%)(P<0.001).(3)Biovar2infectionofU.urealyticumismoreprevalentinsexworkers(28.1%)andSTIoutpatientsgroup(26.6%)thanthatinphysicalcheck-upgroup(4.9%)(P<0.001).(4)MixedinfectioncausedbymorethanoneserotypeofU.urealyticumisincreasingfromphysicalcheck-upgroup(8.6%)toSTIoutpatients(12.4%)andtosexworkers(23.9%)(P<0.01).(5)Thereisnostatisticdifferenceinthedistributionofserotype1,3,and6ofbiovar1amongthesethreegroups(P=0.763).(6)ThePCRmethoddescribedhereisrelativelysimple,rapidandspecificforthebiotypingandserotypingofbiovar1ofU.urealyticum.Conclusion:weshouldpaymoreattentiontobiovar2andmixedinfectionthansingleinfectionofbiovar1ofU.urealyticuminclinicpractice.PCRisagoodmethodinbiotypingandserotyping.