简介:Objective:Squamousesophagealcarcinomaishighlyprevalentindevelopingcountries,especiallyinChina.TuBeiMu(TBM),atraditionalfolkmedicine,hasbeenusedtotreatesophagealsquamouscellcarcinoma(ESCC)foralongterm.tubeimosideI(TBMS1)isthemaincomponentofTBM,exhibitinggreatanticancerpotential.Inthisstudy,weinvestigatedthemechanismofTBMS1cytotoxiceffectonEC109cells.Methods:Comparativenuclearproteomicapproachwasappliedinthecurrentstudyandweidentifiedseveralalteredproteinspots.Furtherbiochemicalstudieswerecarriedouttodetectthemitochondrialmembranepotential,cellcycleandcorrespondingproteins’expressionandlocation.Results:SubcellularproteomicstudyinthenucleusfromEC109cellsrevealedthatalteredproteinswereassociatedwithmitochondrialfunctionandcellproliferation.FurtherbiochemicalstudiesshowedthatTBMS1-inducedmoleculareventswererelatedtomitochondria-inducedintrinsicapoptosisandP21-cyclinB1/cdc2complex-relatedG2/Mcellcyclearrest.Conclusions:ConsideringtheconventionalapplicationofTBMinesophagealcancer,TBMS1thereforemayhaveagreatpotentialasachemotherapeuticdrugcandidateforESCC.
简介:Someantitumoractivitiesofcomponent(E),extractedfromtherootofFagopynumCymosum(Trev)Meisn(FCTM),haverecentlybeendiscoveredinvivoandinvitro.ThecomponentE(CE)’spatternofactionwithtumorcellularDNAatthemolecularpharmacologicallevelwasinvestigatedbymacromolecularsynthesisexperiment(MSE)andhumanDNAinteractionsystemestablishedinourlaboratory.Theexperimentsdemonstratedthat,invitro,theagentcouldmarkedlyinhibittheincorporationof3H-TdRintothecellularDNA,andtheIC50inP388leukemiacellandinSGC-7901cellwas17.86μg/mland110.4μg/ml,respectively.Theagent,atmg/mllevel,couldproduceanintercalationreversionpatternwithDNAwithinashorttime(2hours).Butwhentheintervalwasprolongedforover4hours,theactionchangedtointercalationirreversiblepattern.Accordingtotheseobservations,theauthorsinferthatCEinteractswithDNAintwoways-directlyandindirectly.Theindirectaction,especiallyinlowconcentr
简介:Objective:Toinvestigatetheeffectofbreast-conservationtherapyinearlystagebreastcancer.Methods:Atotalof234earlystagebreastcarcinomapatientsreceivedbreastconservingtreatmentinourhospital.Aftertheoperation,theyunderwentadjuvantchemotherapyandradiotherapy.Allofthesepatientsdesiredtopreservetheirbreasts.Results:Aftermedianfollow-upof29.46months(rangefrom3to100months),3caseshadlocalrelapseand8caseshaddistantmetastasis.Theoverallsurvivalrateof5yearwas96.7%,andthediseasefreesurvivalrateof5yearwas87.85%.Conclusion:Forearlystagebreastcarcinomapatients,classicquadrantectomy,axillarydissectionandpost-operativeadjuvantchemotherapyandradiotherapyleadtoexcellentlocalcontrolandgoodsurvival.
简介:MicroRNAs(miRNAs)aresmall,non-codingsingle-strandedRNAsthatcanmodulatetargetgeneexpressionatposttranscriptionallevelandparticipateincellproliferation,differentiation,andapoptosis.Tcellshaveimportantfunctionsinacquiredimmuneresponse;miRNAsregulatethisimmuneresponsebytargetingthemRNAsofgenesinvolvedinTcelldevelopment,proliferation,differentiation,andfunction.Forinstance,miR-181familymembersfunctioninprogressionbytargetingBcl2andCD69,amongothers.MiR-17tomiR-92clustersfunctionbybindingtoCREB1,PTEN,andBim.ConsideringthatthesuppressionofTcell-mediatedimmuneresponsesagainsttumorcellsisinvolvedincancerprogression,weshouldinvestigatethemechanismbywhichmiRNAregulatesTcellstodevelopnewapproachesforcancertreatment.
简介:目的研究E-cad在食管鳞状细胞癌中的表达及意义。方法免疫组化SABC法检测76例食管癌E-cad的表达。结果正常食管粘膜细胞膜E-cad表达呈阳性。在癌组织中,E-cad细胞膜表达减弱。E-cad减弱表达率为65.8%(50/76),E-cad表达与病理分化程度(P=0.02)、漫润深度(P=0.024)、静脉侵犯(P=0.04)、淋巴结转移(P=0.022)和临床分期(P=0.04)具有相关性;而与年龄、性别、病理类型无关(P>0.05)。在无淋巴结转移组E-cad表达降低占56.25%(27/48),有淋巴结转移组占82.14%(23/28),两者之间表达差异有显著性;有静脉侵犯组E-cad表达降低率为92.86%(13/14)和无静脉侵犯组为59.68%(37/62),两者之间差异有显著性。结论E-cad减弱表达与食管癌生物学行为有关,可能为食管癌预后差的因素之一。
简介:BackgroundSpinalpainisaserioushealthandsocial-economicproblem.Endoscopicspinesurgeryasatreatmentoptionforspinalpainhasgainedtremendousattentionandgrowthinthepast2decades,andavarietyofendoscopictechniqueshavebeeninventedtotreatawiderangeofspinalconditions.PurposesThepurposesofthis2-partreviewarticleareto1)overviewthepublishedtechniquesofendoscopicspinesurgery,2)summarizetheapplicationsofthesetechniquesintreatingvariousspinalconditions,and3)evaluatetheclinicalevidenceofthesafetyandeffectivenessoftheseendoscopictechniquesintreatingsomeofthemostcommonspinalconditions.Thefirstpartofthereviewarticleprovidesanoverviewofcurrentlymostcommonlyusedtechniques.MethodsWesearchedthePubMeddatabaseforpublicationsconcerningendoscopicspinesurgeryandreviewedtherelevantarticlespublishedintheEnglishlanguage.ResultsDiscectomyandforaminotomyarethemostcommontypesofspinesurgerythatcancurrentlybedoneendoscopically.Endoscopictechniqueshavebeenusedtotreatawiderangeofspinaldisorderslocatedinthelumbar,cervical,aswellasthethoracicregionsofthespine.
简介:N^6-methyladenosine(m^6A)isanessentialRNAmodificationthatregulateskeycellularprocesses,includingstemcellrenewal,cellulardifferentiation,andresponsetoDNAdamage.Unsurprisingly,aberrantm6Amethylationhasbeenimplicatedinthedevelopmentandmaintenanceofdiversehumancancers.Alteredm6AlevelsaffectRNAprocessing,mRNAdegradation,andtranslationofmRNAsintoproteins,therebydisruptinggeneexpressionregulationandpromotingtumorigenesis.Recentstudieshavereportedthattheabnormalexpressionofm6Aregulatoryenzymesaffectsm6Aabundanceandconsequentlydysregulatestheexpressionoftumorsuppressorgenesandoncogenes,includingMYC,SOCS2,ADAM19,andPTEN.Inthisreview,wediscussthespecificrolesofm6A“writers",“erasers”,and“readers”innormalphysiologyandhowtheiralteredexpressionpromotestumorigenesis.Wealsodescribethepotentialofexploitingtheaberrantexpressionoftheseenzymesforcancerdiagnosis,prognosis,andthedevelopmentofnoveltherapies.
简介:Glioblastoma(GBM)isoneofthedeadliesttumorsandhasamediansurvivalof3monthsifleftuntreated.Despiteadvancesinrationallytargetedpharmacologicalapproaches,theclinicalcareofGBMremainspalliativeinintent.Sincethemajorityofalteredsignalingcascadesinvolvedincancerestablishmentandprogressioneventuallyaffectcellcycleprogression,analternativeapproachforcancertherapyistodevelopinnovativecompoundsthatblocktheactivityofcrucialmoleculesneededbytumorcellstocompletecelldivision.Inthiscontext,wereviewpromisingongoingandfuturestrategiesforGBMtherapeuticsaimedtowardsG2/Minhibitionsuchasanti-microtubuleagentsandtargetedtherapyagainstG2/Mregulatorslikecyclin-dependentkinases,Aurorainhibitors,PLK1,BUB,1,andBUBR1,andsurvivin.Moreover,wealsoincludeinvestigationalagentsinthepreclinicalandearlyclinicalsettings.Althoughseveraldrugswereshowntobegliotoxic,mostofthemhavenotyetenteredtherapeutictrials.TheuseofeithersingleexposureoracombinationwithnovelcompoundsmayleadtotreatmentalternativesforGBMpatientsinthenearfuture.
简介:Gastrointestinal(GI)cancerisoneofthemostcommoncausesofcancer-relateddeathsworldwide.Tumormarkersarevaluableindetectingpost-surgicalrecurrenceorinmonitoringresponsetochemotherapy.PyruvatekinaseisoformM2(PKM2),aglycolyticenzymecatalyzingconversionofphosphoenolpyruvate(PEP)topyruvate,confersagrowthadvantagetothetumorcellsandenablesthemtoadapttothetumormicroenvironment.Inthisreview,wehavesummarizedcurrentresearchontheexpressionandregulationofPKM2intumorcells,anditspotentialroleinGIcarcinogenesisandprogression.Furthermore,wehavealsodiscussedthepotentialofPKM2asadiagnosticandscreeningmarker,andatherapeutictargetinGIcancer.
简介:目的检查annexinⅠ在食管癌前病变到食管鳞癌不同病变过程中的表达。方法按照研究食管癌早期病变的病理学取材方法取材,选择含有不同程度癌前病变的食管鳞癌标本11例,经连续取材制片和免疫组化分析,比较同一病例由食管癌前病变发展到食管鳞癌的全过程中annexinⅠ的表达改变。结果AnnexinⅠ表达水平在食管正常鳞状上皮中呈强阳性(11/11,100%),低度癌前病变(轻度不典型增生和中度不典型增生)减弱(3/8,37.5%),高度癌前病变(重度不典型增生和原位癌)明显减弱(12/14,85.7/%),食管浸润癌和淋巴结转移癌中阴性或显著减弱(15/15和3/3,100%)。Annex.inⅠ在食管低度癌前病变中的改变具有显著意义(P=0.002)。结论AnnexinⅠ是食管鳞癌早期侯选标志蛋白,在食管低度癌前病变时表达异常。