简介：ThisstudywasundertakentoinvestigatetheregulatoryeffectofResveratrol(Res)ontheproliferationandapoptosisofsynoviocytesofpatientswithrheumatoidarthritis(RA),astheproliferationofsynoviocytesofpatientswasdeterminedbyMTTchromatometryandtheapoptosisofthesecellswasassayedwithTUNELflowcytometry.ItwasfoundinthisexperimentthatthedegreeofcellproliferationoftheRes-treatedgroupwithdosagesof50-400μMwassignificantlyreducedincomparisonwiththatofthecontrolgroup,butpercentageoftheapoptoticcellsdemonstratedwithTUNELlabelingwaselevatedundertreatmentwithResatthesamedosagesinaconcentration-dependentmanner.ThedifferencebetweentheRestreatedgroupandthecontrolgroupwasquitesignificant(P<0.01).ItisconcludedthatResshowsapotentanti-proliferativeeffectonsynoviocytesofpatientswithRAwithinductionofcellapoptosis,anditislikelyavaluablecandidateforthechemotherapyandmanagementofpatientswithRA.

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简介：Toinvestigatetheeffectsofantisenseoligonucleotidesontheexpressionofmacrophagemigrationinhibitoryfactor(MIF)onmacrophages,themousephosphorothioateoligonucleotidesweredesignedandsynthesizedwiththesequencesofantisense,5'-TACGGATACAAGTAGCAC-3'；Sense,5'-ATGC-CTATGTTCATCGTG-3'；Missense,5'-CTCTCAGACTCGATCTGT-3'.Thesephosphorothioateoligonucleotideswerethentransfectedintoculturedmacrophages(RAW264.7)byluciferasevector,andthetransfectedmacrophageswereincubatedwithLipopolysaccharide(LPS)(1ng/ml)forvariousperiodsoftimesandcollectedafterwards.ThecontentofMIFproteinintheculturalsupernatantswasdeterminedbyELISA,cellularRNAextractedandtheexpressionofMIFmRNAwasexaminedbyRT-PCRanalysis.TheexperimentalresultsshowedthatLPScouldinduceatime-dependentspecificexpressionofMIFonmacrophages,inwhichtheMIFmRNAincellsandtheMIFproteininculturalsupernatantsappearedafter3handreachedtheirhighestconcentrationat9-12hafterLPSstimulation.ThelevelsofmRNAandproteinsinthemacrophagestreatedwithantisenseolignucleotidesweredecreasedsignificantlyafterstimulationwithLPSincomparisonwiththatofstimulationwithLPSaloneorwiththatwithLPSplussenseormissenseoligonucleotides.TherewerenodifferencesamongthosewithoutLPSstimulation.ItisconcludedthatmacrophagesstimulatedwithLPSexpressMIF,andtheantisenseolignucleotidesofMIFinhibittheexpressionofMIFmRNAaswellasthesecretionofMIFproteinsinmacrophages.

简介：TheaimofthisstudyistoelucidatethemolecularandcellularmechanismsunderlyingtheimmunosuppressiveeffectofSanchiextract(SE)viainvestigatingtheeffectsofSEontheactivationandproliferationofmurinelymphocytesandNOsecretionbyperitonealmacrophagesinvitro.ConAwasusedtoactivatelymphocytes,andexpressionofCD69onTcellsandCFSElabeledcelldivisionweredetectedbyflowcytometry.MurineperitonealmacrophageswerestimulatedwithLPSorlymphocytesculturesupemate(LCS)andtheconcentrationofNOwasdeterminedbyGriessreagentassay.After6hofculture,SErangingfrom50to100μg/mldownregulatedCD69expressiononConA-activatedTcells,whileSErangingfrom12.5to100μg/mlinhibitedtheproliferativeresponseoflymphocytestoConA.Additionally,SE(12.5-100μg/ml)inhibitedsecretionofNObyperitonealmacrophagesstimu-latedbyLPSorLCS.ThisstudyrevealsthatSEinhibitstheactivationandproliferationofmurinelym-phocytesandNOsecretionbyperitonealmacrophages.

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简介：TheaimofthisstudyistoinvestigatetheeffectsofallitridinontheexpressionoftranscriptionfactorT-bet/GA-TA-3inmiceinfectedbymurinecytomegalovirus.BALB/cmicemodelsystemofmurinecytomegalovirus(MCMV)infectionwasestablished.Inwhich20modelmicewereallocatedrandomlyintoallitridintreatedgroup(n=10)andinfectedcontrolgroup(n=10).Allitridin(25mg·kg^-1·d^-1)wasusedintreatedgroupatthe24hbyintraperitonealroute(once/d×14d),andthesamevolumeofsalinesolutionwasinjectedcontrolmice.Normalcontrolmice(n=10),wereonlygivenwiththesamevolumeof0.89%sodiumchloride,withoutinfectionwithMCMV.TheexpressionlevelsoftranscriptionfactorT-bet/GATA-3mRNAweremeasuredbyRT-PCR,andtheexpressionlevelsofThelper1(Thl)cytokineIFN-γandTh2cytokineIL-10insupernatantofspleencellcultureweremeasuredbyELISA.ExperimentalresultsshowedMCMVinfectioncouldmarkedlydown-modulatetheexpressionofIFN-γandT-bet,andsignificantlyup-modulatetheexpressionofIL-10andGATA-3mRNA.AllitridincouldinduceincreasedexpressionoftranscriptionfactorT-betmRNAandThlcytokineIFN-γsignificantly(P<0.01),anddecreasedexpressionoftranscriptionfactorGATA-3mRNAandTh2cytokineIL-10markedly(P<0.01).ItisconcludedthatMCMVinfectionleadstodisequilibriumofThl/Th2cytokineexpression:thelevelofThlcytokineIFN-γdecreasessignificantlyandTh2cytokineIL-10overexpressesmarkedly.Allitridincanup-regulatetheexpressionofT-betandIFN-γ,andinhibittheexpressionofGATA-3mRNAandIL-10inMCMVinfectedmice,indicatingaTh1dominantstatewhichshouldenhancethespecificcellularimmunereactionsagainstCMVandbehelpfulforclearanceofthecytomegalovirusinhost.

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简介：ToinvestigatetheeffectsofoverallalkaliofatraditionalChinesemedicine“Tongbiling”(brucineandstrychninealkaloidsinmain)onthecytokinesexpressioninTh1andTh2cellsinthesynovialfluidofpatientswithrheumatismarthritisandtheirsignalpathway,themononuclearcellsinthesynovialfluid(SFMC)ofpatientswereisolatedbyFicoll-Hypaquegradientcentrifugation,andtheCD3^+CD69^+andCD3^+HLA-DRantigenwereanalyzedbyflowcytometryincomparisonwiththoseoftheperipheralblood.TherestofcellswereculturedafterresuspensionwithRPMI1640culturemedium.Phorbol12,13-dibutyrate(PDB)andionomycinwereaddedsuccessivelyintotheculturewithvariousconcentrationofoverallalkaliTongbiling(TBL).After4hofcultivation,theexpressionofIFN-γandIL-4inCD3^+cellswereanalyzedbyflowcytometry.TheinfluenceofoverallalkaliTBL(100mg/I,)ontheintracellularcalciumwasinvestigatedafterFluo-3/AMlabelingandstimulationwithPDBandionomycinat1,2,4and10min,andtheinfluenceofTBLontheexpressionofCD3^+CD69^+cellsweredeterminedwithstimulationofPDBfor24hinthewholebloodlymphocytesculture.ItwasfoundthatthepercentageofTcellsbearingCD69wassignificantlyup-regulated(77%),whilethatofTcellsbearingHLA-DRwas44%inthesynovialmononucleatedcells.AfterPDBandionomycinstimulation,theexpressionofIFN-7inCD3~cellswereup-regulated,buttherewasnochangeontheexpressionofIL-4inCD3^+cells,indicatingthatratioofTh1/Th2wassignificantlyincreasedandThcellsdifferentiatetoThlcellsinmainly.FourconcentrationsofoverallalkaloidofTBI,(200mg/L,100mg/L,50mg/L,25mg/L)coulddown-regulatedtheexpressionofIFN-γinCD3^+cellsandtheTh1/Th2ratioobviously,butalltheconcentrationsoftheoverallalkaloidshadnoeffectontheexpressionofIL-4inCD3^+cells.100mg/Lconcentrationoftheoverallalkaloiddidnotdown-regulatetheintracellularcalciumlevel.Eachconc

简介：Toinvestigatetheinfluenceofmycophenolatemofetil(MMF)uponthematurationandtheallo-stimulatoryactivityofculturedprogenitorsofdendriticcells(DCp),andtoevaluatetheeffectsofthepre-treateddentriticcellsofrecipientswithMMFonthetoleranceinductionaswellasitspossiblemechanism,GM-CSFandMMFwereaddedtotheinvitroculturedprogenitorcells,andtheimmuno-phenotypicalanalysiswasperformedbymeansofflowcytometry.ThesecretionofIL-12wasdetectedbyELISAandthestimulatoryactivitiesofDCponallogeneicTcellswereobservedbymixedlymphocytereaction.Twenty-fourC57BL/6miceweredividedinto3groups(eachwith8mice),inwhichgroupAofmiceacceptedallograftsofheartfromBALB/cmice,groupBofmicehadreceiveduntreatedDCpfromdonorsofBALB/cmice7daysbeforetransplantation,andC57BL/6miceingroupCweretreatedbyinjectionwithMMF-treatedallograftsofheartfromBALB/cmice7daysbeforetransplantation.Thesurvivaltimesofallograftsandthechangesofthecytokinelevelsinsereoftherecipientmicewereobservedaftertransplantation.TheexperimentalresultsshowedthatMMFcouldsignificantlyinhibittheexpressionsofthecostimulatorymoleculesCD80andCD86onDCsandthesecretionofIL-12andtheallo-stimulatoryactivitiesofDCswerealsomarkedlyinhibited.ThesurvivaltimesofallograftsingroupBofmicewerelongerthanthoseingroupA,whilethegroupCshowedthelongestsurvivaltimesofallografts,withamarkedreductionintheproductionoftheThltypecytokines.ItisevidentthatMMFhasasuppressiveeffectonthematurationandallo-stimulatoryactivitiesofthecultureddendriticcellprogenitors,thusleadingtoadonorspecifictoleranceinheart-transplantedrecipients.