简介:TheeffectsofBacillussubtilis2-1fromtheintestineofhealthyseacucumberonthegrowth,digestiveenzymeactivitiesandintestinalmicrobiotaofjuvenileseacucumber(Apostichopusjaponicus)weredeterminedinthepresentstudy.SeacucumberwasfedwithSargassumthunbergiipowdersupplementedwithB.subtilis2-1atdifferentconcentrationsvaryingamong0(control),105,107,and109CFUg-1for8weeks.ResultsshowedthatthegrowthperformanceandintestinalamylaseandtrypsinactivitiesweresignificantlyincreasedbydietaryB.subtilis2-1at109CFUg?1(P<0.05).However,dietaryB.subtilis2-1hadnosignificantinfluenceonthelipaseactivityinseacucumber(P>0.05).Thepolymerasechainreactiondenaturinggradientgelelectrophoresisand16SrRNAgenesequencinganalysisindicatedthatdietaryB.subtilis2-1at105and107CFUg-1inhibitedmostoftheProteobacteriaincludingthoseingenusVibrio.DietaryB.subtilis2-1at109CFUg-1notonlydecreasedtheabundanceandspeciesofgenusVibrio,butalsoincreasedtheintensityofgeneraPsychrobacterandBacillus.AspecificdosageofdietaryB.subtilis2-1couldincreasethegrowthandmodulatetheintestinalmicrobiotaofseacucumber;thusitmightbeanovelprobioticforkeepingthehealthofseacucumber.
简介:本研究从独立学院应用型本科人才培养的视角入手,尝试编制自主学习能力测评量表对电气类专业学生进行测评,利用主成分分析法等统计学方法进行分析,讨论影响自主学习能力测评的主要影响因素。经主成分分析,提取到的6个特征值均大于1的主成分,累计贡献率达60.86%,其中通过第1主成分便可较清晰地将自主学习能力得分群体分开。本研究视角下,得到影响大学生自主学习能力的关键指标体系,即职业信念、自我管理、学习管理3个维度,职业兴趣、职业环境认知、职业定位、职业准备、专业认知、职业能力认知、自我调整、时间管理、目标管理、学习态度、信息能力、学习方法12种影响因子。