Objective:Tostudycellularcompatibilityofimprovedscaffoldmaterialwithdeproteinizedheterogeneousboneandprovideexperimentalbasisonchoosingthescaffoldmaterialinbonetissueengineering.Methods:Bonemarrowstromalcells(BMSC)wereco-culturedwithheterogeneousdeproteinizedboneinvitro.Thecontrastphasemicroscope,scanningelectronmicroscope,MTTassay,flowcytometrywereperformedandtheBGPcontentandALPactivitiesweredetectedinordertoobservethecellgrowth,adhesioninthematerial,cellcycleandcellviability.Results:Thescaffoldmaterialofdeproteinizedheterogeneousbonehadnoinhibitoryeffectoncellularproliferation,differentiationandsecretionfunctionofBMSCs.Conclusions:TheestablishedheterogeneousdeproteinizedbonehasgoodbiocompatibilitywithBMSCsandisapotentiallyidealscaffoldmaterialforbonetissueengineering.