Thepropertiesofimmobilizedglucoseoxidase(GOD)bythecomplexesofdiethylaminoethylcellulose(DEAEC)withdifferentpolymers,suchaspolymethylacrylicacid(PMAA),polyacrylicacid(PAA),polystyrenesulfonicacid(PSSA),polyvinylaleohol(PVA),polyethyleneoxide(PEO)andstyrene-maleicacidcopolymer(PSMA)wereinvestigated.TheactivityofimmobilizedGODwasobviouslyinfluencedbythecomponentoftheDEAECcomplexes.TherelativeactivityoftheimmobilizedGODreachedtomaximumandover90%ofthenativeGOD.whentheDEAEC-PMAADEAEC-PAAcomplexeswereusedasacarrierwiththemolarratioofDEAECandpolyacidofaboutone.Michaelisconstants(Km)oftheimmobilizedenzymesofDEAEC-GOD-PMAAandDEAEC-GOD-PAAweredeterminedtobe1.25and1.00,respectively.Moreover,theimmobilizedGODhasagoodstoragestabilityandcycliclife.
