简介:<正>19.PengchengDelicacyMonthDate:October1-31Place:XuzhouCity,JiangsuProvinceWhatson:1,AtourofthehomelandofChina’soriginatorofcooking.PengZuiscommonlyknownastheearliestancestorofChinesechefsandtheoriginatorofcooking.AtourofPengZu’shomelandwilltakeitasthethemeof"seekingtheancienttracesofPengZuandtastingthefoodcreatedbyhim."2.ImperialPalaceBanquetandDelicacyFestivalofHannationality.Thankstothestudyof
简介:Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilicmontmorillonite(PHBV/OMMT)nanocompositeswerepreparedandthebiodegradabilityofthePHBV/OMMTnanocompositeswasstudiedbyacultivationdegradingmethodinsoilsuspension.TherelationshipbetweenstructureandbiodegradabilityofPHBV/OMMTnanocompositeswasinvestigated.TheresultsshowedthatthebiodegradabilityofPHBV/OMMTnanocompositesdecreasedwithincreasingamountofOMMTanditwasrelatedtothenumberofPHBVdegradingmicroorganismsindegradationenvironment,theanti-microbialpropertyofOMMTandthedegreeofcrystallinityofthenanocomposites.
简介:目的建立具有潮霉素(hygromycin)抗性的3T3细胞系,用于转染目的基因(pTRE-Ins-human)的ES阳性细胞克隆筛选的饲养层.方法通过脂质体转染的方法,将含有潮霉素B磷酸转移酶基因的质粒pHyg导入3T3细胞中,利用潮霉素的药物选择特性,对转染细胞进行压力筛选,并对其进行PCR鉴定.结果经500μg/ml的潮霉素压力筛选后,获得了抗性细胞克隆.抗性3T3细胞的形态和生长速度与正常3T3细胞没有差异,特异性核苷酸引物检测抗性细胞基因组DNA,可以扩增出对应的核苷酸片段.结论成功地培育了潮霉素抗性的3T3细胞,为进行目的基因(pTRE-Ins-human)转染ES细胞的阳性细胞克隆筛选奠定了基础.
简介:Aim:ToobservetheapoptoticchangesfollowingexposuretoEMPandtoexplorethepossibleinjurymechanisminNIH-3T3fibroblasts.Methods:FollowingNIH-3T3cellswereexposedtoEMP,theproliferationandviabilityofNIH-3T3fibroblastswereestimatedbyhemacytometerandMTTMeasurements.Apoptoticratewasmeasuredbyflowcytometer.TheimnmohistochemicalSPmethodwasusedtodeterminebcl-2,p53.ThepositivelystainedcellswereanalyzedwithCMIAS-Ⅱimageanalysissystematamagnification400.AlldatawereanalyzedbySPSS8.0software.Results:TheproliferationandviabilityofNIH-3T3cellsweremarkedlyinhibitedandsignificantapoptosiswasinducedfollowingexposuretoEMP.EMPcouldincreasethenumberofnon-adherentcells,thehighestratio(33.9%)ofnon-adherentcellsalsooccurredat6h.TheAs70valueofMTTdecreasedimmediatelyat1h,6hfollowingthecellswereexposedascomparedwiththecontrol(P<0.05).Thehighestratioofapoptosiswas15.07%at6h,thendecreasedgradually.Down-regulationofbcl-2andup-regulationofp53wereinducedbyEMP(P<0.05).Conclusions:EMPcouldpromoteapoptosisofNIH-3T3fibroblasts.EMPcouldalsodown-regulatebcl-2levelandup-regulatep53levelinNIH-3T3fibroblasts.Bcl-2andp53genemaytakepartintheprocessofapoptosis.
简介:介绍了氢氟烃CF4CH2CHF2的生产工艺。该过程包括:有催化剂存在时于气相中在较高温度下对CF3CH2CF3脱氢氟化。该催化剂包括:(1)氟化铝;(2)氟化氧化铝;(3)含氟离子的3价铝化合物上载的金属;(4)氟化镧;(5)氧氟化镧;(6)含氟离子的2价镧化合物上载的金属;(7)价铬化合物,和/或(8)由(a)、(b)随意加入组成的,(a)选自镁、锌、或镁锌混合物的氧化物、氟化物和氧氟化物的至少一种化合物,(b)至少一种铝的氧化物、氟化物和氧氟化物,且上述催化剂中铝相对镁和锌总量的摩尔比为约1:4或更少。通过上述工艺生产的产品中含CF3CHCF2和HF;有HF存在时,在含氢气相中于氢化催化剂上氢化CF3CHCF2生成CF3CH2CHF2。