简介：Objective:Toinvestigatetheexpressionofmatrixmetalloproteinase-7(MMP-7)andFasligand(FasL)ingastriccancerandexploretheirroleinprogressionofgastriccancer.Methods:Formalin-fixedparaffinandembeddedtissuesofprimarygastriccancerandadjacentnon-tumormucosafrom113caseswereevaluatedforMMP-7,FasLandCapase-3expressionbystreptavidin-peroxidase(S-P)immunohistochemistry.Theexpressionofthefirsttwoproteinsincancercellsofprimaryfociwascomparedwithclinicopathologicalparametersoftumors.WealsoobservedthecorrelationofMMP-7andFasLexpressionwithCaspase-3expressionincancercellsofprimaryfoci.Results:MMP-7positiveimmunostainingwaslessfrequentlydetectedinadjacentepithelialcellsthanincancercellsofprimaryfociofgastriccancer(P<0.05,29.2%vs69.0%),andsowasFasL(P<0.05,34.5%vs54.0%).MMP-7expressionwasassociatedwithtumorsize,Borrmann'sclassification,invasivedepth,metastasisandTNMstaging(P<0.05),butnotwithgrowthpattern,Lauren'sclassification,orhistologicalclassification(P>0.05).FasLexpressionwascorrelatedwithtumorsize,invasivedepth,metastasis,Lauren'sclassification,histologicalclassification(P<0.05),whilenotwithBorrmann'sclassification,TNMstagingorgrowthpattern(P>0.05).CancercellsofprimaryfociexpressedlessCaspase-3thantheiradjacentepithelialcells(P<0.05,32.7%vs50.4%).TherewasanobviouscorrelationbetweenFasL,MMP-7andCaspase-3expressionincancercellsofprimaryfoci(P<0.05).Co-expressionofMMP-7andFasLparalleledwithCaspase-3expressionincancercellsofprimaryfoci(P<0.05).Conclusion:MMP-7andFasLexpressionwasup-regulatedingastriccarcinogenesisandwasprincipallyinvolvedinprogressionofgastriccancer.FasLexpressioncouldreflectthedifferentiationofgastriccancercellsandunderliethemolecularmechanismsofdifferentpathwaysofgastrictumorigenesis.Co-expressionofMMP-7andFasLcouldhaveapoptosis-inducingeffe

简介：背景：超声(美国)指导了锁骨的在上或下文锁骨的块通常为上面的极限外科被使用。这使随机化的研究的目的是比较块表演和发作时间，有效性，不利事件和病人的发生是指导美国的在上的接受锁骨或下文锁骨的块。我们假设了在上锁骨的途径，更表面、更容易设想使用10MHz变换器，将生产一更快并且更广泛的感觉的块。方法：120个病人被使随机化到二个相等的组：在上锁骨的(S)和下文锁骨的(I)。每个病人收到了包含ropivacaine的相等的体积的混合物7.5mg/ml和mepivacaine有肾上腺素5μg/ml的20mg/ml，0.5ml/kg身体重量(最小30ml，最大值50ml)。七根终端神经的感觉分数(0削尖的麻醉2点，痛觉缺失1点和疼痛)被估计每10min。当他们在肘下面有有效的外科的块麻醉或五根神经的痛觉缺失时，病人们为外科被宣布准备好了。在块以后的三十分钟，解块的神经被补充。块表演和潜伏时间，外科的有效性，不利事件和病人是接受被记录。结果：显著地，在I的更多的病人组织为在块以后的外科20和30min准备好了。吝啬的块表演时间在I组(NS)是在S组和5.0min的5.7min。块有效性在I组是优异的：93%对78%在S组(P=0.017)。S组病人有中部、尺骨的神经，而是腋的神经的更好的块的显著地更差的块。在10，20和30min的感觉分数不是显著地不同的。在S组的32个病人对在I组的九个病人经历了短暂不利事件(P<0.0001)。病人块的接受在两个组是类似的。结论：Infraclavicular块有更快的发作，更好外科的有效性和更少不利事件。块表演时间和病人是过程的接受在两个组是类似的。

简介：AbstractBackground:Previous studies have revealed that diabetes mellitus (DM) promotes disease progress of gastric cancer (GC). This study aimed to further investigating whether DM advanced lymph nodes (LNs) metastasis in GC.Methods:The clinicopathologic data of GC patients with >15 examined LN (ELN) between October 2004 and December 2019 from a prospectively maintained database were included. The observational outcomes included the number (N3b status) and anatomical distribution (N3 stations) of metastatic LN (MLN).Results:A total of 2142 eligible patients were included in the study between October 2004 and December 2019. N3 stations metastasis (26.8% in DM vs. 19.3% in non-DM, P = 0.026) and N3b status (18.8% in DM vs. 12.8% in non-DM, P = 0.039) were more advanced in the DM group, and multivariate logistic regression analyses confirmed that DM was an independent factor of developing N3 stations metastasis (odds ratio [OR] = 1.771, P= 0.011) and N3b status (OR= 1.752, P= 0.028). Also, multivariate analyses determined DM was independently associated with more MLN (β = 1.424, P = 0.047). The preponderance of N3 stations metastasis (DM vs. non-DM, T1-2: 2.2% vs. 4.9%, T3: 29.0% vs. 20.3%, T4a: 38.9% vs. 25.8%, T4b: 50.0% vs. 36.6%; ELN16-29: 8.6% vs. 10.4%, ELN30-44: 27.9% vs. 20.5%, ELN ≥ 45: 37.7% vs. 25.3%), N3b status (DM vs. non-DM, T1-2: 0% vs. 1.7%, T3: 16.1% vs. 5.1%, T4a: 27.8% vs. 19.1%, T4b: 44.0% vs. 28.0%; ELN16-29: 8.6% vs. 7.9%, ELN30-44: 18.0% vs. 11.8%, ELN ≥ 45: 26.4% vs. 17.3%), and the number of MLN (DM vs. non-DM, T1-2: 0.4 vs. 1.1, T3: 8.6 vs. 5.2, T4a: 9.7 vs. 8.6, T4b: 17.0 vs. 12.8; ELN16-29: 3.6 vs. 4.6, ELN30-44: 5.8 vs. 5.5, ELN ≥ 45: 12.0 vs. 7.7) of DM group increased with the advancement of primary tumor depth stage and raising of ELN.Conclusions:DM was an independent risk factor for promoting LN metastasis. The preponderance of LN involvement in the DM group was aggravated with the advancement of tumor depth.

简介：Objective:Tocreateanomogramtopredicttheincidenceoflymphnodemetastasis(LNM)inearlygastriccancer(EGC)patientsandtoexternallyvalidatethenomogram.Methods:Toconstructthenomogram,weretrospectivelyanalyzedaprimarycohortof272EGCpatients.Univariateanalysisandabinarylogisticregressionwereperformed.AnomogrampredictingtheincidenceofLNMinEGCpatientswascreated.Thediscriminationabilityofthenomogramwasmeasuredusingtheconcordanceindex(c-index),andthenomogramwasalsocalibrated.Then,anotherprospectivecohortof81patientswasanalyzedtovalidatethenomogram.Results:Intheprimarycohort,LNMwaspathologicallyconfirmedin37(13.6%)patients.Inmultivariateanalysis,thepresenceofanulcer,themaximumlesiondiameterobservedviagastroscopy,thethicknessofthelesionobservedviaendoscopicultrasonography,andthepresenceofenlargedlymphnodesoncomputedtomography(CT)wereindependentriskfactorsforLNM.Anomogramwasthencreatedbasedontheregressionmodelwiththec-indexof0.905,andthecalibrationcurveofthenomogramfellapproximatelyontheideal45-degreeline.Thecut-offscoreofthenomogramwas110,andthesensitivity,specificity,positivepredictiveandnegativepredictivevaluesofthenomogramintheprimarycohortwere81.1%,86.0%,47.6%and96.7%,respectively,andintheprospectivevalidationcohortwere75.0%,91.0%,60.0%and95.5%,respectively.Thecalibrationcurveoftheexternalvalidationcohortwasalmostonthe45-degreeline.Conclusions:WedevelopedaneffectivenomogrampredictingtheincidenceofLNMforEGCpatients.

简介：Objective:Humaninducedpluripotentstem(iPS)cellsexhibitgreatpotentialforgeneratingfunctionalhumancellsformedicaltherapies.Inthispaper,wereportforuseofhumaniPScellslabeledwithfluorescentmagneticnanoparticles(FMNPs)fortargetedimagingandsynergistictherapyofgastriccancercellsinvivo.Methods:HumaniPScellswerepreparedandculturedfor72h.Theculturemediumwascollected,andthenwascoincubatedwithMGC803cells.CellviabilitywasanalyzedbytheMTTmethod.FMNP-labeledhumaniPScellswerepreparedandinjectedintogastriccancer-bearingnudemice.Themousemodelwasobservedusingasmall-animalimagingsystem.Thenudemicewereirradiatedunderanexternalalternatingmagneticfieldandevaluatedusinganinfraredthermalmappinginstrument.Tumorsizesweremeasuredweekly.Results:iPScellsandthecollectedculturemediuminhibitedthegrowthofMGC803cells.FMNP-labeledhumaniPScellstargetedandimagedgastriccancercellsinvivo,aswellasinhibitedcancergrowthinvivothroughtheexternalmagneticfield.Conclusion:FMNP-labeledhumaniPScellsexhibitconsiderablepotentialinapplicationssuchastargeteddual-modeimagingandsynergistictherapyforearlygastriccancer.

简介：AbstractBackground:Gastric cancer (GC) is one of the most globally prevalent cancers in the world. The pathogenesis of GC has not been fully elucidated, and there still lacks effective targeted therapeutics. The influence of altered kinesin superfamily protein 22 (KIF22) expression in GC progression is still unclearly. The aim of this study was to investigate the KIF22 effects on GC and related mechanisms.Methods:Gastric carcinoma tissues and matching non-cancerous tissues were collected from patients with GC who have accepted a radical gastrectomy in Lanzhou University Second Hospital from May 2013 to December 2014. The expression of KIF22 was examined in GC of 67 patients and 20 para-carcinoma tissues by immunochemical staining. The relationship between the expression of KIF22 and clinicopathologic characteristics was next investigated in the remaining 52 patients except for 15 patients who did not complete follow-up for 5 years. Cell viability was performed via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test and colony formation assay in the MGC-803 and BGC-823 GC cells. Cell scratch and trans-well invasion assay was performed to assess migration ability in the MGC-803 and BGC-823 GC cells. Gene set enrichment analysis (GSEA) pathway enrichment analysis was performed to explore the potential functions. Cell cycle was detected by flow cytometry. In addition, the two GC cell lines were used to elucidate the underlying mechanism of KIF22 in GC in vitro via assessing the effects on mitogenactivated protein kinase and extracellular regulated protein kinases (MAPK/ERK) signal transduction pathway-related expressions by Western blotting assays. The differences were compared by t tests, one-way analysis of variance, and Chi-squared tests.Results:The study showed that KIF22 was up-regulated in GC, and KIF22 high expression was significantly related to differentiation degree (χ2 = 12.842, P = 0.002) and poorly overall survivals. GSEA pathway enrichment analysis showed that KIF22 was correlated with the cell cycle. Silence of KIF22 decreased the ability of the proliferation and migration in gastric cells, induced G1/S phase cell cycle arrest via regulating the MAPK-ERK pathways.Conclusions:KIF22 protein level was negatively correlated with prognosis. KIF22 knockdown might inhibit proliferation and metastasis of GC cells via the MAPK-ERK signaling pathway.

简介：AIM:TOexplorethefeasibilityofenhancingapoptosis-inducingeffectsofchemotherapeuticdrugsonhumangastriccancercellsbystabletransfectionofextrinsicSmacgene.METHODS:AfterSmacgenewastransferredintogastriccancercelllineMKN-45,subclonecellswereobtainedbypersistentG418selection.CellularSmacgeneexpressionwasdeterminedbyRT-PCRandWesternblotting.Aftertreatmentwithmitomycin(MMC)asanapoptoticinducer,invitrocellgrowthactivitieswereinvestigatedbytrypanblue-stainingmethodandMI-Icolorimetry.Cellapoptosisanditsratesweredeterminedbyelectronicmicroscopy,annexinV-FITCandpropidiumiodidestainingflowcytometry.Cellularcaspase-3proteinexpressionanditsactivitieswereassayedbyWesternblottingandcolorimetry.RESULTS:WhencomparedwithMKN-45cells,theselectedsubclonecelllineMKN-45/SmachadsignificantlyhigherSmacmRNA(3.12±0.21vs0.82:1:0.14,t=7.52,P<0.01)andproteinlevels(4.02±0.24vs0.98:1:0.11,t=8.32,P<0.01).Aftertreatmentwith10μg/mLMMCfor6-24h,growthinhibitionrateofMKN-45/Smac(15.8±1.2-54.8±2.9%)wassignificantlyhigherthanthatofMKN-45(5.8±0.4-24.0±1.5%,t=6.42,P<0.01).PartialMKN-45/Smaccancercellspresentedcharacteristicmorphologicalchangesofapoptosisundertheelectronicmicroscopewithanapoptosisrateof36.4=1=2.1%,whichwassignificantlyhigherthanthatofMKN-45(15.2±0.8%,t=9.25,P<0,01).ComparedwithMKN-45,caspase-3expressionlevelsinMKN-45/Smacwereimprovedsignificantly(3.39±0.42vs0.96:1:0.14,t=8.63,P<0.01),whileitsactivitieswere3.25timesasmanyasthoseofMKN-45(0.364±0.010vs0.112:1:0.007,t=6.34,P<0.01).CONCLUSION:StabletransfectionofextrinsicSmacgeneanditsover-expressioningasbiccancercelllinecansignificantlyenhancecellularcaspase-3expressionandactivities,ameliorateapoptosis-inducingeffectsofmitomycinConcancercells,whichisanovelstrategytoimprovechemotherapeuticeffectsongastriccancer.

简介：Objective:TheUnionforInternationalCancerControl(UICC)Node(N)classificationisthemostcommonusedstagingmethodfortheprognosisofgastriccancer.Itdemandsadequate,atleast16lymphnodes(LNs)tobedissected;thereforedifferentstagingsystemswereinvented.Methods:BetweenMarch2005andMarch2010,164patientswereevaluatedattheDepartmentofGeneralSurgeryintheKenézyGyulaHospitalandattheDepartmentofGeneral,ThoracicandVascularSurgeryintheKaposiMórHospital.The6th,7thand8thUICCN-stagingsystems,thenumberofexaminedLNs,thenumberofharvestednegativeLNs,themetastaticlymphnoderatio(MLR)andthelogoddsofpositiveLNs(LODDS)weredeterminedtomeasuretheir5-yearsurvivalratesandtocomparethemtoeachother.Results:Theoverall5-yearsurvivalrateforallpatientswas55.5%withamedianoverallsurvivaltimeof102months.Thetumorstage,gender,UICCN-stages,MLRandtheLODDSweresignificantprognosticfactorsforthe5-yearsurvivalwithunivariateanalysis.The6thUICCN-stagedidnotfollowtheadequateriskincomparingN2vs.N0andN3vs.N0withmultivariateinvestigation.ComparisonofperformancesoftheresidualNclassificationsprovedthattheLODDSsystemwasfirstinthepredictionofprognosisduringtheevaluationofallpatientsandincaseswithlessthan16harvestedLNs.TheMLRgavethebestprognosticpredictionwhenadequate(morethanorequalto16)lymphadenectomywasperformed.Conclusions:WesuggesttheapplicationofLODDSsystemroutinelyinwesternpatientsandtheusageofMLRclassificationincaseswithextendedlymphadenectomy.

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简介：在病人作为首要或第二线的治疗评估paclitaxel，cisplatin和5-FU(PCF)的联合政体的功效和毒性与的目的进展胃并且在中国的esophagogastric连接(EGJ)腺癌。病人与paclitaxel被对待的方法d1上的150mg/m2；fractionatedcisplatin15mg/m2和连续注入5-FU600mg/(m2朠楬污映扩楲汬牡????????????М

简介：AIM:Tocomparetwotypesofclassificationofintestinalmetaplasia(IM)ofthestomachandtoexploretheirrelationshiptogastriccarcinoma.METHODS:Forty-sevencasesofgastricIMwereclassifiedintotypeortypeaccordingtomucinhistochemicalstainingandcomparedwithanovelclassificationinwhichthespecimenswereclassifiedintosimpleIM(SIM)oratypicalIMaccordingtopolymorphismintermsofatypicalchangesofthemetaplasticepithelium.Forty-sevenIMandthirty-sevengastriccarcinomasa...

简介：AIM:Toinvestigatetheassociationbetweenendogenousgeneexpressionandgrowthregulationincludingproliferationandapoptosisinducedbytransforminggrowthfactor-β1(TGF-β1)inhumangastriccancer(GC)cells.METHODS:Reversetranscriptionpolymerasechainreaction(RT-PCR)wasperformedtodetectthemaincomponentsoftheTGF-β1/SmadssignalpathwayinhumanpoorlydifferentiatedGCcelllineBGC-823.LocalizationofSmadproteinswasalsodeterminedusingimmunofluorescence.Then,theBGC-823cellswereculturedinthepresenceorabsenceofTGF-β1(10ng/mL)for24and48h,andtheeffectsofTGF-β1onproliferationandapoptosisweremeasuredbycellgrowthcurveandflowcytometry(FCM)analysis.TheultrastructuralfeaturesofBGC-823cellswithorwithoutTGF-β1treatmentwereobservedundertransmissionelectronmicroscope.Theapoptoticcellswerevisualizedbymeansoftheterminaldeoxynucleotidyltransferase(TdT)-mediateddTUPinsitunickend-labeling(TUNEL)method.Meanwhile,theexpressionlevelsofendogenousp15,p21andSmad7mRNAandthecorrespondingproteinsinthecellsweredetectedat1,2and3haftercultureinthepresenceorabsenceofTGF-β1(10ng/mL)bysemi-quantitativeRT-PCRandWesternblot,respectively.RESULTS:TheTGF-β1/SmadsignalingwasfoundtobeintactandfunctionalinBGC-823cells.ThegrowthcurverevealedthemostevidentinhibitionofcellproliferationbyTGF-β1at48h,andFCMassayshowedG1arrestaccompaniedwithapoptosisinducedbyTGF-β1.ThetypicalmorphologicalchangesofapoptosiswereobservedincellsexposedtoTGF-β1.Theapoptosisindex(AI)inTGF-β1-treatedcellswassignificantlyhigherthanthatintheuntreatedcontrols(10.7±1.3%vs0.32±0.06%,P＜0.01).Thelevelsofp15,p21andSmad7mRNAandcorrespondingproteinsincellsweresignificantlyup-regulatedat1h,butgraduallyreturnedtobasallevelsat3hfollowingTGF-β1(10ng/mL)treatment.CONCLUSION:TGF-β1affectsbothproliferationandapoptosisofGCcellsth

简介：AbstractBackground:The neoadjuvant chemotherapy is increasingly used in advanced gastric cancer, but the effects on safety and survival are still controversial. The objective of this meta-analysis was to compare the overall survival and short-term surgical outcomes between neoadjuvant chemotherapy followed by surgery (NACS) and surgery alone (SA) for locally advanced gastric cancer.Methods:Databases (PubMed, Embase, Web of Science, Cochrane Library, and Google Scholar) were explored for relative studies from January 2000 to January 2021. The quality of randomized controlled trials and cohort studies was evaluated using the modified Jadad scoring system and the Newcastle-Ottawa scale, respectively. The Review Manager software (version 5.3) was used to perform this meta-analysis. The overall survival was evaluated as the primary outcome, while perioperative indicators and post-operative complications were evaluated as the secondary outcomes.Results:Twenty studies, including 1420 NACS cases and 1942 SA cases, were enrolled. The results showed that there were no significant differences in overall survival (P = 0.240), harvested lymph nodes (P = 0.200), total complications (P = 0.080), and 30-day post-operative mortality (P = 0.490) between the NACS and SA groups. However, the NACS group was associated with a longer operation time (P < 0.0001), a higher R0 resection rate (P = 0.003), less reoperation (P = 0.030), and less anastomotic leakage (P = 0.007) compared with SA group.Conclusions:Compared with SA, NACS was considered safe and feasible for improved R0 resection rate as well as decreased reoperation and anastomotic leakage. While unbenefited overall survival indicated a less important effect of NACS on long-term oncological outcomes.

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简介：TostudythemechanismofinfectionofEpstein-Barrvirus(EBV)ingastriccarcinomacells,theAkataandP3HR-1strainsofEBVwereusedastheteststrainsofviruses,andthesignetringcelllineHSC-39ofgastriccarcinomacellswasusedasthetargetcellsofinfection.Thevirus-infectedcellcloneswereisolatedbylimiteddilutionmethod.ItwasfoundthattheEBV-encodedsmallRNA(EBER)couldbedetectedintheinfectedcells.TheAkataandP3HR-1EBVinfectedparentalcellsandmostofclonesexpressedEBNA1,butnotEBNA2.Latentmembraneprotein(LMP-1)andLMP-2,andtheQpromoter(p),butnottheCp/WpforEBNAgenetranscriptionwasactiveintheinfectedparentalcellsaswellasalltheclones.UninfectedHSC-39cellsdidnotexpressCD21,however,AkatabutnotP3HR-1EBV-infectedclonesex-pressedlowlevelofCD21mRNA.TheseresultsdemonstratethatHSC-39cellsaresusceptibletobothEBVstrainsandEBVinfectsHSC-39cellsthroughtheCD21-independentpathway.ThisstudydefinesasignetringtypeofgastriccarcinomacellslineasauniquetargetcellsforthestudyofEBVinfectionmechanism.

简介：瞄准：在胃的很好区分的内分泌的肿瘤(GWDET)(ECL房间良性肿瘤)调查cyclin依赖的激酶禁止者p21和p27的表示。方法：p21和p27的表情从匹配GWDET的诊断标准的16个病人在内视镜的活体检视标本immunhistochemically被检验。弱或强壮的积极原子染色任何一个的百分比被注意。有年龄，性，肿瘤本地化，multifocality和伴随的长期的萎缩性胃炎，神经内分泌房间增生(NEH)，神经内分泌发育异常(NED)，肠的组织变形(IM)，Ki-67增长索引和临床的结果的免疫表情的协会也被评估。结果：所有情况与43.6%的一个吝啬的表示分数表示了p27，当31.3%案例显示出任何p21表示时。p21和p27免疫表情显著地与对方一起被相关(P<0.01)，并且表示p21组有更高的p27表示分数(68%对22%)。p21和p27表情在女人是更低的，在在某处除没有粘膜下层扩展的宫底以外，其肿瘤被定位的非衰退的粘膜和盒子。在上矛盾，p21和p27表情与粘膜下层扩展和萎缩性胃炎在男性和病人是更高的。介绍更低的p27分数的盒子有独居的肿瘤显示出既不NEH-NED也不IM。尽管有，有更低的p21表示的盒子介绍了NEH-NED伴随的多焦点的肿瘤。然而，p21的关联和p27表情都没与年龄和Ki-67表示被发现。结论：p27广泛地在GWDET被表示，当p21表示在三分之二个盒子中稀少、观察时。p21和p27表情的损失可以与不同良性肿瘤肿瘤子类型被相关；然而，更多的研究被需要在胃肠的内分泌的肿瘤估计这些未来的标记的角色。

简介：Purpose:Wntpathwayscontrolkeybiologicalprocessesthatpotentiallyimpactontumorprogressionandpatientsurvival.Thepresentstudyanalyzedthepolymorphismoflipoprotein-relatedreceptor5(LRP5)(genewithkeyfunctionsinWntsignaling)anditsimpactontheresponsetochemotherapyandsurvivalofpatientswithadvancedgastriccancer(AGC).Methods:Atotalof107consecutivepatientswithAGCtreatedwithfirst-linechemotherapyofEOFregimenwereenrolledinthepresentretrospectivestudy.Theassociationbetweensinglenucleotidepolymorphism(SNP)ofrs3736228inLRP5andtheclinicaloutcomesofthepatientswasstudied.Results:TheCCgenotypeofrs3736228wassignificantlycorrelatedwithahigherdiseasecontrolratewhencomparedtotheCTandTTgenotypes(89.3%and61.8%,respectively,P<0.001).Aunivariatesurvivalanalysisalsoshowedthattheprogressionfreesurvival(PFS)andoverallsurvival(OS)forthepatientswiththeTCandTTgenotypesofrs3736228wereworsethanforthepatientswiththeCCgenotype(PFS:3.3and6.7months,respectively,HR=0.454,P<0.001;OS:8.1monthsand18.8months,respectively,HR=3.056,P<0.001).AmultivariateCoxmodelincorporatesrs3736228andclinicalfeatures,alsoidentifiedrs3736228wassignificantlyassociatedwiththePFSandOS.Conclusions:OurresultsfirstlyhighlighttheimportanceofLRP5geneofWntpathwayinthetreatmentofAGCandidentifypolymorphismofrs3736228asindependentpredictorofdiseasecontrolrate,PFSandOSinAGCpatientstreatedwithfirst-linechemotherapyofEOFregimenintheChineseHanpopulation.

简介：Objective:Todeterminetheclinicalserumlevelsofcarcinoembryonicantigen(CEA)andcarbohydrateantigen19-9(CA19-9),individuallyandincombination,forthediagnosisof50healthysubjectsand150casesofesophageal,gastric,andcoloncancers.Methods:Thesensitivitiesofthetwomarkerswerecomparedindividuallyandincombination,withspecificitysetat100%.Receiveroperatingcharacteristic(ROC)curveswereplotted.Results:SerumCEAlevelsweresignificantlyhigherincancerpatientsthaninthecontrolgroup.ThesensitivityofCEAwasdetermined:inesophagealcancer,sensitivity=28%,negativepredictivevalue(NPV)=61.72%,andAUC=0.742(SE=0.05),withasignificancelevelofP<0.0001;ingastriccancer,sensitivity=30%,NPV=58.82%,andAUC=0.734(SE=0.05),withasignificancelevelofP<0.0001;incoloncancer,sensitivity=74%,NPV=79.36%,andAUC=0.856(SE=0.04),withasignificancelevelofP<0.0001.ThesensitivityofCA19-9wasalsoevaluated:inesophagealcancer,sensitivity=18%,NPV=54.94%,andAUC=0.573(SE=0.05),withasignificancelevelofP=0.2054.Ingastriccancer,sensitivity=42%,NPV=63.29%,andAUC=0.679(SE=0.05),withasignificancelevelofP<0.0011.Incoloncancer,sensitivity=26%,NPV=57.47%,andAUC=0.580(SE=0.05),withasignificancelevelofP=0.1670.ThefollowingwerethesensitivitiesofCEA/CA19-9combined:inesophagealcancer,sensitivity=42%,NPV=63.29%,SE=0.078(95%CI:0.0159-0.322);gastriccancer,sensitivity=58%,NPV=70.42%,SE=0.072(95%CI:-0.0866-0.198);andcoloncancer,sensitivity=72%,NPV=78.12%,SE=0.070(95%CI:0.137-0.415).Conclusion:CEAexhibitedthehighestsensitivityforcoloncancer,andCA19-9exhibitedthehighestsensitivityforgastriccancer.Combinedanalysisindicatedanincreaseindiagnosticsensitivityinesophagealandgastriccancercomparedwiththatincoloncancer.

简介：ToinvestigatetheprotectiveeffectofdecoctionofliaoweiongastricmucosaofH.pyloriassociatedchronicatrophicgastritisinrats.TheH.pyloriassociatedchronicatrophicgastritismodelswereinducedwithsodiumdeoxycholate,sodiumsalicylate,ethanol,andculturebrothofH.pylori.ProtectiveeffectofdecoctionofliaoweiongastricmucosaofH.pyloriassociatedchronicatrophicgastritiswasobservedquantitatively.

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