简介：Inthisstudy,weaimedtoexploretheroleofursolicacidintheneuralregenerationoftheinjuredsciaticnerve.BALB/cmicewereusedtoestablishmodelsofsciaticnerveinjurythroughunilateralsciaticnervecompletetransectionandmicroscopicanastomosisat0.5cmbelowtheischialtuberosity.Thesuccessfullygeneratedmodelmiceweretreatedwith10,5,or2.5mg/kgursolicacidviaintraperitonealinjection.Enzyme-linkedimmunosorbentassayresultsshowedthatserumS100proteinexpressionlevelgraduallyincreasedat1-4weeksaftersciaticnerveinjury,andsignificantlydecreasedat8weeks.Assuch,ursolicacidhasthecapacitytosignificantlyincreaseS100proteinexpressionlevels.Real-timequantitativePCRshowedthatS100mRNAexpressionintheL4-6segmentsontheinjurysidewasincreasedafterursolicacidtreatment.Inaddition,themuscularmassindexinthesoleusmusclewasalsoincreasedinmicetreatedwithursolicacid.Toluidinebluestainingrevealedthatthequantityandaveragediameterofmyelinatednervefibersintheinjuredsciaticnerveweresignificantlyincreasedaftertreatmentwithursolicacid.10and5mg/kgofursolicacidproducedstrongereffectsthan2.5mg/kgofursolicacid.Ourfindingsindicatethatursolicacidcandose-dependentlyincreaseS100expressionandpromoteneuralregenerationinBALB/cmicefollowingsciaticnerveinjury.

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简介：Besidesthebiologicalgrowthfactors,smallorganicmolecules,andchemicalions,physicalsignalsistheothercategoryofveryimportantfactorstotune/regulatethefateofstemcells.Recentyears,moreattentionhasbeenpaidonthedifferentiationofstemcellsonthephysicalsignal,including,electricormagneticfield,surfacetopologyofbiomaterials,photoirradiation,andevenpressureandstrainfromthematerials.With

简介：骨头髓(BM)的细胞的基础织物开发和新生通过造血的干细胞(HSC)和间充质的干细胞(MSC)被调停。在造血的房间和BMstromal房间(BMSC)之间的本地相互影响在myelosuppression以后决定造血作用的体质。这里，我们在侮辱以后控制BM新生考察BM本地信号。造血的生长因素(HGF)和BMSC生产的cytokines是在BM造血作用的规定的主要因素。对在多重种类的早胚胎开发批评的Morphogens被加到HSC管理者的家庭，包括Wnt蛋白质，槽口ligands，BMP，和刺猬的家庭。HSC和BMSC的全球基因表示分析开始为两种房间类型揭示了基因的签名组。更重要地，生物化学、生物的结合的全球基因表示的分析在BM期间本地信号学习新生强烈建议了HGF和cytokines不能是为BM恢复的主要本地管理者，chemokines(SDF-1，FGF-4)并且angiogenic生长因素(VEGF--一，Ang-1)在myelosuppression以后在BM宪法起有启发性的作用。BM毒性的管理的一个新方向从BM再生管理者的鉴定正在出现。

简介：Directexposuretointensivevisiblelightcanleadtosolarretinopathy,includingmacularinjury.Thesignsandsymptomsincludecentralscotoma,metamorphopsia,anddecreasedvision.However,therehavebeenfewstudiesexaminingretinalinjuryduetointensivelightstimulationatthecellularlevel.Neuralnetworkarrangementsandgeneexpressionpatternsinzebrafishphotoreceptorsaresimilartothoseobservedinhumans,andphotoreceptorinjuryinzebrafishcaninducestemcell-basedcellularregeneration.Therefore,thezebrafishretinaisconsideredausefulmodelforstudyingphotoreceptorinjuryinhumans.Inthecurrentstudy,thecentralretinalphotoreceptorsofzebrafishwereselectivelyablatedbystimulationwithhigh-intensitylight.Retinalinjury,cellproliferationandregenerationofconesandrodswereassessedat1,3and7dayspostlesionwithimmunohistochemistryandinsituhybridization.Additionally,alight/darkboxtestwasusedtoassesszebrafishbehavior.Theresultsrevealedthatphotoreceptorswereregeneratedby7daysafterthelight-inducedinjury.However,theregeneratedcellsshowedadisruptedarrangementatthelesionsite.Duringtheinjury-regenerationprocess,thezebrafishexhibitedreducedlocomotorcapacity,weakenedphototaxisandincreasedmovementangularvelocity.Thesebehaviorsmatchedthemorphologicalchangesofretinalinjuryandregenerationinanumberofways.Thisstudydemonstratesthatthezebrafishretinahasarobustcapacityforregeneration.Visualimpairmentandstressresponsesfollowinghigh-intensitylightstimulationappeartocontributetothealterationofbehaviors.

简介：ComparedwiththespeciesofLeucesection,thedifficultyinplantregenerationduringtissuecultureforthespeciesofAigeirossectionisakeylimitingfactorfortheirapplicationingeneticengineeringofforesttrees.Inthisstudy,theplantgrowthregulatorscombination,copperconcentration,lightintensity,andtheselectionpressuresofkanamycinwereinvestigatedusingleavesofPopulus×euramericanacl.’Bofeng’asexplants,andastableandhighefficientregenerationsystemwasestablished.Usingthissystem,boththeshootregenerationrateandrootingratewereupto100%,andtheaveragenumberofdifferentiatedshootsineachleafexplantwasupto20,andthesurvivalrateofseedlingsreached98%.Theoptimalselectionpressureofkanamycininshootinducementforleaf-explantwas40mg·L-1,andtheoptimalselectionpressureofkanamycininrootinducementofadventitiousbudwas20mg·L-1.TheauthorsalsofoundthatdifferentiationrateofadventitiousbudsofP.×euramericanacl.’Bofeng’couldbesignificantlypromotedbyincreasedcopperconcentration,andthisisthefirstreportaboutthekeyroleofcopper（Cu）inexplantregenerationofPoplars.ThisregenerationsystemwithhighfrequencywhichwasestablishedbytheauthorswasasgoodasthatofspeciesofLeucesection,anditprovidesagoodtechnologicalplatformforgeneticengineeringofforesttreewhichusesthespeciesofAigeirossectionasmodelmaterials.

简介：Inanefforttodevelopbiomaterialstomeetguidedtissueregeneration(GTR)standardsforperiodontaltissuerecovery,ahomogeneousandtransparentchitosan(CS)/hydroxyapatite(HA)membranewithpotentialapplicationsasGTRbarrierinperiodontaltherapyhasbeenpreparedviainsitucompositing.ThemembranehasbeendesignedtohaveasmoothroughasymmetricstructurethatmeetsthedemandforGTR.ComponentandmorphologyofthemembranearecharacterizedbyXRDandSEM.ItcanbeindicatedthatHAwasinsitusynthesizeduniformlyintheCSmembrane.MechanicalexperimentsofthemembraneswithvariousHAcontentsshowthattheirtensilestrengthsareadequateforperiodontaltherapy.Biologicalpropertiesofthemembranehavebeenperformedbycelltoxicityassays,hemolysistestsandanimalexperiments.Resultsindicatethatthemembranehasgoodbiocompatibilityandinductiveeffectforcellgrowth.ThereforethismembranecanbepotentiallyappliedasGTRbarriermembraneforperiodontaltissueregeneration.

简介：Byinvestigation,thethesisanalysesandsummarizestheforminganddevelopingofCHFRPInXupucounty.WeselecttheMayandongstockcooperationforestfarmasobject,adoptthemethodssuchassemi-structureinterview,analysingseconddataandsurveyingsampleplots,analyseandstudytheprocesstoclarifythepropertyright,theprofitsallocatedformsandthemanagementforms.TheresultsshowthatCHFRPcantentativelyresolvethecontradictionbetweentheforestlandusingrightscatteredtoeve...

简介：Multiplesclerosis(MS)isachronicimmune-mediatedinnammatory-demyelinatingdisorderofthecentralnervoussystem,withastrongneurodegenerativecomponent.ThequestionwhetherneurodegenerationinMSisindependentorrelatedtoneuroinflammationhasbeenlongdebated,butnotyetfullyclarified.Furthermore,littleisstillknownonhowneuroinflammationandneurodegenerationinMSarerelatedtopotentialregenerativeprocesses.Inthisperspective,webrieflydiscussmainclinical,pathologicalandexperimentalevidenceontherelationshipbetweenneuroinflammationandneurodegenerationinMS,andontheirconnectionwithregeneration.WediscussthattheseprocessesinMSmightrepresentintercorrelatedmanifestationsoftheimmuneresponse,especiallyoftheinnateimmunity.

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简介：Inordertoselectasuitablegenotypeforthestudiesongenetictransformation,thedifferenceamongthreegenotypes(DN04-18,DN04-25andDN04-26)ofPopulus×euramericana(Populusdeltoides×P.nigra)intermsofshootregenerationwasinvestigatedinthispaper.AnefficientinvitromicropropagationmethodwassuccessfullyestablishedforgenotypeDN04-18.Thehighfrequencyshootregeneration(90.0%)fromleafdiscswasobtainedingenotypeDN04-18onthemodifiedMurashigeandSkoog(mMS)mediumw...

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简介：Traumaticbraininjury(TBI)istheleadingcauseofdeathanddisabilityofpersonsunder45yearsoldintheUnitedStates,affectingover1.5millionindividualseachyear.Ithadbeenthoughtthatrecoveryfromsuchinjuriesisseverelylimitedduetotheinabilityoftheadultbraintoreplacedamagedneurons.However,recentstudiesindicatethatthematuremammaliancentralnervoussystem(CNS)hasthepotentialtoreplenishdamagedneuronsbyproliferationandneuronaldifferentiationofadultneuralstem/progenitorcellsresidingintheneurogenicregionsinthebrain.Furthermore,increasingevidenceindicatesthattheseendogenousstem/progenitorcellsmayplayregenerativeandreparativerolesinresponsetoCNSinjuriesordiseases.Insupportofthisnotion,heightenedlevelsofcellproliferationandneurogenesishavebeenobservedinresponsetobraintraumaorinsultssuggestingthatthebrainhastheinherentpotentialtorestorepopulationsofdamagedordestroyedneurons.Thisreviewwilldiscussthepotentialfunctionsofadultneurogenesisandrecentdevelopmentofstrategiesaimingatharnessingthisneurogeniccapacityinordertorepopulateandrepairtheinjuredbrain.

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简介：Theirretrievablefateofneuronsdominatedtheneurosciencerhetoricforthefirsthalfofthiscentury,apositionthatwasfiercelycontestedandrecentlydebunkedbyextensivestudiescarriedoutinthefieldofneuroregenerationresearch.Theturningpointcameintheyear1928,whenRamonY.Cajal’s(Lobato,2008)worksuggestedthattheregenerativecapacityof

简介：AbstractSkeletal muscle plays an essential role in generating the mechanical force necessary to support the movement of our body and daily exercise. Compared with cardiac and smooth muscle, in mammals, skeletal muscle exhibits remarkable regenerative capacity in response to damage. Muscle stem cells, also known as satellite cells, directly contribute to regeneration. Here, we review primary and secondary myogenesis processes with a focus on muscle stem cells, as well as the function and regulation of muscle stem cells in adult muscle regeneration in mammals.

简介：Thisstudyproposesapost-processortoimprovetheharmonicstructureofavowelinanenhancedspeech,enablingthespeechqualitytobeimproved.Initially,aspeechenhancementalgorithmisemployedtoreducethebackgroundnoiseforanoisyspeech.Hencetheenhancedspeechispost-processedbyahybrid-medianfiltertoreducethemusicaleffectofresidualnoise.Sincetheharmonicspectraareimpactedbybackgroundnoiseandaspeechenhancementprocess,thequalityofavowelisdeteriorated.Aharmonicregeneratedmethodisdevelopedtoimprovethequalityofpost-processedspeech.Experimentalresultsshowthattheproposedmethodcanimprovethequalityofpost-processedspeechbyadequatelyregeneratingharmonicspectra.

简介：Tinnitus,thephenomenonofringingorbuzzingintheearswithoutanexternalsoundsourceisoneofthemostcommonlyreportedsymptomsinotorhinolaryngologyandaffects10–15%ofthegeneralpopulation.Modelshavebeendevelopedtoaccountforneuralbasisoftinnitus,itspathogenesisanditsconsequencesonmentalhealth(deRidderetal.,2013).Inmostcasestinnitusonsetfollowsapartialhearingimpairment.Peripheralsensorydeprivationduetocochleardamages

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