简介：Inthispaper,weproposeaparametricmodelingmethodtoreconstructtheHui-Styleandsolvetheproblemthattheusercan’teffectivelyreconstructtheHui-StylethreedimensionalmodelsbecauseofthevariousstyleandcomplicatedstructureofHui-Stylecomponents.ThismodelincludessummarizinganddefiningavarietyofHui-Stylecomponentsparametertypes.Determinetherelationshipbetweentheparameteraccordingtobuildingformulasanddesigntheparametricmodelingprocess.First,setthedepthofplatformasthegivenuservalue.Then,calculatethecomponentpropertiesandthecorrespondingsizeaccordingtothestyleoftheHui-StylearchitecturecomponentsandtheconstraintrelationbetweenHui-Stylearchitecturecomponents.Thisneedsonlyoneparameterthatisneededasthebasicparameterinthismethodandtheentiremodelingprocesscanbeachievedbasedonit.TheexperimentalresultshowsthattheproposedmethodcanwellsolvethecomplexparametersrelationshipoftheHui-StylearchitecturecomponentsandhelpthegeneraluserstoconstructtheHui-Stylearchitecturecomponentsmoreeffective.

简介：Arithmeticcodingisarelativelynewloss-lessdatacompressiontechniquethathasattractedmuchattentioninrecentyears.Weshowtheiterationofbit-levelarithmeticcodingcanbespecifiedbyacontinuousfunction.Theanalysisexpressionandsomepropertiesofthisfunctionarediscussed.Anapplicationofthefunctionisprovidedforexploringthesecurityofarithmeticcodeswhentheyareusedfordataencryption.

简介：客观：在在人的创伤的奔流和正常透镜的上皮的房间之间的原子factor-KB(NF-κB)的表示学习差别。方法：全部的RNAof前面的囊标本从受不了创伤的奔流做半量的RT-PCR并且在在他们之间的NF-κB的表示进行差别的分析的正常cadaveric眼睛施主和那些在显微镜下面被拿。结果：作为与在正常控制组的0.8337的平均数相比，NF-κB的表示等价物为在创伤的奔流患者的透镜的上皮的房间是0.9074，并且差别具有显著意义(t=2.447，P<0.05)因此。结论：NF-κB是可能的对必要的一种抄写因素维持正常透镜的上皮的房间的新陈代谢。在创伤的奔流患者可得到的更高的NF-κB“透镜的上皮的房间工具NF-κB具有到创伤的奔流的出现和开发的可能的关联的s。

简介：Objective:TostudytheimmunologicalmechanismsofCondylomaacuminata(CA)throughinvestigatingTlymphocytesubsetlevelsandcytokineprofileintheperipheralbloodofpatientswithCondylomaAcuminata.Methods:Tricolorandbicolorimmunofluorescentstainingantibodyofcellsurfaceantigenandintracel-lularIL-2,IL-4,IL-12,IFN-γinCD4^+andCD8^+T-lymphocytesfrom20patientswithCAwereperformedandfollowedbyflowcytometry.Results:ThenumberofCD3^+T,CD4^+T-lymphocytescellsandCD4^+/CD8^+Tcellsratioweresignificantlydecreased(P<0.01)inpatientswithCAComparedtocontrols,andIL-2,IL-12,IFN-γproductioninCD4^+Tcellswasdecreased(P<0.01),IL-4andIFN-γproduc-tioninCD4^+Tcellswasnotsignificantlydifferent(P>0.05),whileIL-2andIL-12productioninCD8^+Tccellswasdecreased(P<0.01),whereasIFN-γandIL-4pro-ducinginCD4^+Tcellswereofnosignificantlydifference(P>0.05).Conclusions:TherewasanimbalanceofTlympho-cytesubsets,Th1/Th2cytokinesandTc1/Tc2intheperipheralbloodofCApatients,whichmayplayanimportantroleinthepathogenesisandprogressionofCA.

简介：在大多数谷物庄稼，phytic酸是磷的主要存储形式，它能减少磷酸盐的简历可获得性。phytase的转基因的表示被认为是在转基因的植物免除磷酸盐phytate的一个有效方法。在这研究，工厂表示向量，包含重组体phytase基因由玉米ubiquitin(Ubi)开车倡导者经由Agrobacterium-mediatedtransformation被构造并且介绍进一个精英米饭变化。在实验期间，15根独立转基因的米饭线的一个总数被改革。PCR和南部的污点的结果显示目标基因集成于转基因的米饭工厂的染色体。而且，提取fromtheimmature几根转基因的线播种的全部的RNA的RT-PCR分析证明重组体phytase基因能通常被表示。无机的磷内容，两个都比在untransformed野生型在转基因的工厂在成熟种子和叶是显著地更高的。

简介：Ourobjectiveistosolvethelactosemalabsorptionandintoleranceofhumanbeingsbycombiningmlcro-ecologypathwithgeneticengineeringtechnique.PlasmidpMG36ewasusedtocloneandexpressaβ-galactosidasegenefromL.delbrueckiibulgaricusstrain1.1480intheLactococcuslactissubsp,cremorisMG1363andLactococcuslactissubsp.lactisIL1403.TherecombinantplasmidwaspreservedandproliferatedinEscherichiacoli(E.coli)JM109,andtransformedintoMG1363and1L1403byelectroporation.Theproteinexpressionwasstudied.(1)Thebifidobacteriumculturemedium(BBL)wassuitableforthegrowthofthestrain1.1480.(2)With13aminoacidsattheN-terminusfromthevector,β-galactosidasefusionprotein(whichretainedtheenzymeactivity)couldbesuccessfullyexpressedinE.coliJM109,MG1363andIL1403,buttheexpressionquantitywaslargerintheformerthaninthelattertwo.(3)TheSDsequencedesignedcouldbesuccessfullyrecognizedbyboththeE.coliandtheLactococcuslactis,buttheexpressionlevelofthenon-fusionβ-galac-tosidaseproteinwaslowerthanthatofthefusionproteininthesamehost.Theβ-galactosidasegeneticallyengineeredE.coliJM109isausefultooltoproducethisenzymeinvitro.Thesignalpeptideoftheusp45proteinfromtheLactococcuslactiscanbeaddedbeforethepromotersequencetopromoteβ-galactosidasesecretionfromLactococcuslactis.Thepotentialapplicationoftheβ-galactosidasegeneticallyengineeredMG1363andIL1403tocurethelactosemalabsorptionandlactoseintoleranceinbothhealthfoodandmedicineispromising。

简介：基因的表示“在正确地方以内”并且在正确时间被限制，这通常被相信。这个原则长也被认为适用到免疫球蛋白(Ig)的表示B的淋巴细胞房间系。然而，增加证据证明了Ig“似非而是地”在上皮的起源的恶意的肿瘤表示了。我们在导出癌症的Ig的学习考察了最近的进步，并且也讨论了它的机制和可能的功能，试着引起到那些在免疫学和肿瘤学的领域里工作的兴趣和注意。

简介：几种细胞间的粘附分子是，这以前被报导仔细与长期的HBV感染有关。CD2和CD58的建筑群在提高T淋巴细胞的粘附到目标房间，并且支持T淋巴细胞的增生和激活起一个重要作用。在这研究，我们检测了在PBMC的表面上表示的CD2的水平，在PBMC和在有长期的HBV感染的病人的PBMC的CD2积极房间的百分比的CD2mRNA的表达式水平并且把他们与正常控制的表达式水平作比较。我们也与长期的HBV感染并且从正常控制从病人决定了浆液HBVDNA的水平。肝的功能的临床的特征也被测试。结果证明CD2的表示显著地与长期的HBV感染的严厉增加了，它建议CD2可能在长期的HBV感染贡献hepatocyte损坏。

简介：Duetoaproductionerror,somemistakeshavebeenintroducedinlabelsofFigure1C,Figure2C,andFigure4B.The

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简介：Thenaturalmeasureofacertainareainphasespaceisdefinedfirstly.Onthebasisofnaturalmeasure,theexpressionofLyapunovexponentbasedonunstableperiodicorbits(UPOs)ofchaoticsystemsisdeducedfromtheoreticalaspect.Then,bymeansoftheinherentrelationbetweenUPOsandsystematicLyapunovexponent,thetransitionalmechanismandrouteofchaoticsystemsfromlow-dimensionalchaostohigh-dimensionalchaosareexplained.Intheend,anovelmethodforcomputingsystematicLyapunovexponentsbasedonUPOsisproposed.Itscomputingprocedureisalsosummarized.ThechaoticsystemdescribedbyHenonmapistakenasexample.ThroughcalculatingtheLypunovexponentsofthissystem,validityofthesuggestedmethodisverified.

简介：Thecommonapproachtofindco-regulatedgenesistoclustergenesbasedongeneexpression.However,duetothelimitedinformationpresentinanydataset,genesinthesameclustermightbeco-expressedbutnotnecessarilyco-regulated.Inthispaper,weproposetointegrateknowntranscriptionfactorbindingsiteinformationandgeneexpressiondataintoasingleclusteringscheme.Thisschemewillfindclustersofco-regulatedgenesthatarenotonlyexpressedsimilarlyunderthemeasuredconditions,butalsosharearegulatorystructurethatmayexplaintheircommonregulation.Wedemonstratetheutilityofthisapproachonamicroarraydatasetofyeastgrownunderdifferentnutrientandoxygenlimitations.Ourintegratedclusteringmethodnotonlyunravelsmanyregulatorymodulesthatareconsistentwithcurrentbiologicalknowledge,butalsoprovidesamoreprofoundunderstandingoftheunderlyingprocess.Theaddedvalueofourapproach,comparedwiththeclusteringsolelybasedongeneexpression,isitsabilitytouncoverclustersofgenesthatareinvolvedinmorespecificbiologicalprocessesandareevidentlyregulatedbyasetoftranscriptionfactors.

简介：人的脐的绳索血(CB)最近在移植被用作干细胞的来源。从CB导出的NK房间是涉及graft-versus-host疾病(GVHD)和graft-versus-leukemia(GVL)的关键受动器房间。CBNK房间的活动比成年的外部血(PB)的低，这被报导NKcells.In这研究，我们在CB和PBNK房间分析了一些NK房间受体和cytotoxicity相关的分子的表示。激活NK受体，CD16，NKG2D和NKp46的表情，没显示出CB和PBNK房间之间的重要差别。但是禁止的受体NKG2A/CD94的表示在CBNK房间上是显著地更高的。至于受动器功能分子，granzymeB被表示在表面FasL和小道没显示出的细胞内部的perforin，IFN-，TNF-和房间的CBNK房间，而是表情显著地更低CB和PBNK房间之间的差别。结果显示NKG2A/CD94的高表示和granzymeB的低表示可能与CBNK房间的减少的活动是相关的。

简介：ThepresentstudyisaimedatstudyingthegeneforTIMP-3,amammaliantissueinhibitor,byconstructingarecombinanteukaryoticcellvectorforgenetherapyinhumanbreastcancer.WeobtainedtheTIMP-3genefromthehumanplacentbyRT-PCR.TIMP-3genewassubclonedintopcDNA3.1vetorfrompMD18TvectorbymeansofgenecloningtoconstructpcDNA3.1recombinantvector.HumanbreastcancercelllineMDA-MB-453wastransfectedwithpcDNA3.1-TIMP3recombinantvectorusinglipofectaminereagent.ThentheexpressionofTIMP-3andtheeffectonthemetastasisofMDA-MB-453wereexamined.ThecorrectconstructionofpcDNA-TIMP3wasidentifiedbymeansofrestrictionenzymeanalysis,PCRamplicationandnucleotidesequencing.WesternblottingshowedthatthetransfectedcellswereabletoexpressTIMP-3,indicatingthatourconstructionofthepcDNA-TIMP3eukaryoticexpressionvectorwasconstructedsuccessfully.OurexperimentsfurtherindicatedthatthepotentialofmetastasiswassignificantlyreducedforthetransfectedcelllineMDA-MB-453.

简介：Objective:TostudythebiologicalactivityofMycoplasmapenetrans35kDalipoprotein(P35)invitro,prokaryoticexpressionvectorpQE31/p35wasconstructedandrecombinantfusionproteinP35(rP35)wasexpressedinE.coli.Methods:Thep35genewasamplifiedbypolymerasechainreaction(PCR),clonedtopQE31,andapositiveclonewasscreened.PCR-mediatedmutagenesiswasusedtochangethetwo"TGA"tripletsto"TGG"tripletswithinthep35gene.ProductionoftherecombinantproteinwasinducedbytheadditionofIPTGtotheE.coliculture,rP35waspurifiedwithaNi-NTASpinKitandrP35purificationwasanalyzedbyWesternblot.Results:About1KbPCRamplificationwasclonedintopQE31.Thetwo"TGA"tripletswithinthep35geneweresuccessfullychangedto"TGG"triplets.ThepQE31/p35vectorexpressedaproteinwithacalculatedmolecularmassof37.4kDainE.coli.Westernblotindicatedthe37.4kDaproteinwasrP35.Conclusion:PQE311p35,aprokaryoticexpressionvectorcontainingp35gene,wassuccessfullyconstructedandexpressedinE.coli.

简介：AbstractBackground:Hyperbaric oxygen treatment (HBOT) has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids. To explore the mechanism of the effect of HBOT on keloids, tumor immune gene expression and immune cell infiltration were studied in this work.Methods:From February 2021 to April 2021, HBOT was carried out on keloid patients four times before surgery. Keloid tissue samples were collected and divided into an HBOT group (keloid with HBOT before surgery [HK] group, n = 6) and a non-HBOT group (K group, n = 6). Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit. Data were mined with R package. The differentially expressed genes between the groups were compared. Hub genes between the groups were determined and verified with Quantitative Real-time PCR. Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry (IHC).Results:Inflammatory cell infiltration was reduced in the HK group. There were 178 upregulated genes and 217 downregulated genes. Ten hub genes were identified, including Integrin Subunit Alpha M (ITGAM), interleukin (IL)-4, IL-6, IL-2, Protein Tyrosine Phosphatase Receptor Type C (PTPRC), CD86, transforming growth factor (TGF), CD80, CTLA4, and IL-10. CD80, ITGAM, IL-4, and PTPRC with significantly downregulated expression were identified. IL-10 and IL-2 were upregulated in the HK group but without a significant difference. Infiltration differences of CD8 lymphocyte T cells, CD4 lymphocyte T-activated memory cells, and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis. Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.Conclusion:HBOT affected tumor gene expression and immune cell infiltration in keloids. CD4 lymphocyte T cell, especially activated memory CD4+T, might be the key regulatory immune cell, and its related gene expression needs further study.

简介：Object:ToidentifytranscriptvariantsandexpressionpatternsofporcineMitf.Materialsandmethods:ApairwiseBLASTsearchatNCBIdatabasewasperformedtodeducethestructureofporcineMitfgene.Subsequently,50RACEandfluorescentquantitativeRT-PCRwereusedtoanalyzetheexpressionpatternofporcineMitfindifferenttissues.Results:FourtranscriptvariantsofporcineMitf,MITF-A,MITF-H,MITF-MandMITF-SUSwereidentified,allsharinghighhomologywiththoseinhumans,exceptMitf-SUS.Conclusion:ThesequenceofporcineMitfappearhighlyhomologoustohumanMITF.However,only4transcriptvariantsofporcineMitfwereidentifiedintheseminipigs,lessthanthe9transcriptvariantsinhumanMITF.

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简介：Objective:Toachieveanoptimizedmethodforsolubleexpressionofhumancarboxylesterase1(hCE-1)inescherichiacoilandpurificationbyNi2+-NTAagaroseaffinitychromatography,togetimprovedproteinyieldandpurityforfurtherdevelopmentofhepatocellularcarcinoma(HCC)diagnosisELISAkits.Methods:ThebestantigenepitopesofhCE1werepredictedbycomparingsecondarystructure,flexibleregions,hydrophilicity,antigenicindexsurfaceprobabilityofresidues.Afterwards,pET-42a(+)withaHis-tagandaGST-tagwasappliedtoformrecombinantplasmidpET-42a(+)/hCE1,whichfacilitatedpurificationwhenusingNi2+-NTAagaroseaffinitychromatography.ProteinqualitywasmeasuredbySDS-PAGEandBCAproteinassay.Western-blotidentificationwasalsoperformedtoensurethecorrectexpressionofhCE1protein.Results:Theresiduesfrom500to567nearC-terminalofhCE1proteinwereconsideredthebestepitopeswhichexhibitedhighhydrophilicityandhighsurfaceprobabilityandrelativelyflexiblesecondarystructureandlowhomologycomparedwithhCE2andhCE3.His-hCE1500-567fusionproteinwasachievedbyIPTG-inductedexpressionwithanexpectedmassof42kDa.Afterpurification,thefinalproductwasspeciallyidentified,whichreachedover95%purityandmorethan10mg/Lofmicrobialculture.InWesternblot,thepurifiedfusionproteinwasrecognizedbyanti-hCE1monoclonalantibody,alongwithprevioussequencingvalidation,whichdemonstratedthecorrectpreparationofsolublehCE1protein.Conclusion:ThisisanefficaciousandaffordablestrategytogeneratefusionhCE1ofhighqualityinEcoli,whichfacilitatespreparationofhCE1monoclonalantibodyandfurtherHCCdiagnosisresearch.

简介：Objective:Toinvestigatetheexpressionofimmune-relatedmoleculesinglioblastomamultiform(GBM)cells.Methods:Theexpressionofmajorhistocompatibilitycomplex(MHC),β2-microglobulin,Fas,CD80andCD86moleculesonthesurfaceofGBMcellswereevaluatedbyflowcytometry.TheexpressionofTAP-1,TAP-2andTapasinintheGBMcellswereevaluatedbyRT-PCRmethod.Results:MHCclassⅠ,β2microglobulin,TAP-1,TAP-2andtapasinwereexpressedinmostGBMcelllines.ExceptU87,therewasnoMHCclassⅡmoleculeexpressiononanyoftheotherGBMcelllines.FaswasexpressedonalltheGBMcelllinesexamined.Conclusion:ThemechanismbywhichGBMescapesimmunesurveillancemayinvolvedownregulationofexpressionofMHCclassⅠmoleculesandMHCclassⅡmolecules.MHCclassⅠpositiveGBMmaybethesuitabletargetofimmunotherapy.