简介:AbstractObjective:The aim of this work was to measure the antibacterial activity (against Escherichia coli and Staphylococcus aureus [S. aureus]) of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups: negative control group [with Mueller-Hinton broth (MHB)], positive control group (with 75% ethanol), blank group (with MHB) and test group (with the ethyl acetate part of Abrus cantoniensis). The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration (MIC). Time-kill curve experiments, scanning electron microscopy, the content of DNA, RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S. aureus. The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University (approval No. XJTULAC2016-412) on January 22, 2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S. aureus with an inhibition zone diameter of 16.4 mm and MIC value of 0.5 μg/mL. The general activity range of the ethyl acetate part, determined using a time-killing curve, was found to be 0.5 μg/mL to 40 μg/mL (MIC to 80 × MIC). Changes in the scanning electron microscopy images and of DNA, RNA and proteins of S. aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures, which results in protoplasm leakage, and eventually bacterial cell death.
简介:无
简介:摘要:社会的不断进步与发展给现阶段小学教育领域带来了新的机遇和挑战,随着社会需求的不断发展对于学生的整体评价也越来越严格。相较于传统的教学思想,现阶段小学英语教育目标已不仅仅局限在学生考试成绩的提高,学生的综合能力越来越受到人们关注。作为小学英语教学的组织者和重要参与者,教师在学生素质英语素质形成过程中发挥着重要角色,其教学理念的革新直接关系到学生学习和发展方向。现阶段小学英语教学强调提高学生的核心素养,作为英语教学的重要组成部分,小学英语对话教学对于提高学生核心素养意义重大,为了帮助学生不断提高对话技巧并不断完善其综合素质,教师要在核心素养背景下积极探索提高英语对话教学的有效手段。
简介:【摘要】在英语对话课教学中,利用好思维导图,有助于学生提取文本主题信息,同时为师生对话或生生对话提供语汇;有助于学生提炼文本语言结构,进行新旧知识整合,建立起合理知识网络,发展学生思维品质;有助于活化文本语言运用,提高学生学习能力和语言运用能力。
简介:摘要:直入式讲授法已经没有多大的吸引力,而英语情境教学能大大的吸引学生的眼球,