简介：Studyonbiotinylationstrategiesforcompetitiveimmunoassayofestradiol(E2)wascarriedout.Twotypesofcompetitiveenzymeimmunoassay(EIA)withBiotin-Avidinamplificationsystemwereestablishedandoptimized.TheE2-Biotinconjugatewasusedasatracerinoneassay,andbiotinylatedantibodywasusedasatracerintheother.InbothofEIAs,horseradishperoxidase-labelledAvidin(Avidin-HRP)wasusedwithaspectrometricdeterminationofenzymeactivity.Theprecision,sensitivityandspecificityweremeasuredandcompared.Theresultsshowedthatalthoughbothweresatisfactoryinspecificity,theEIAwithhapten-BiotinshowedtobesuperiortotheEIAwithbiotinylatedantibodyinsensitivityandprecision.ThelimitofdetectionofserumE2was8and50pg/mLwithE2-Biotinandbiotinylatedantibodyastracer,respectively.

简介：Objective:ToevaluatethevalueofenhancedluminescenceenzymeimmunoassayinthediagnosisofNeisseriagonorrhea(NG)infection.Methods:Anti-catalaseantibodyforNeisseriagonorrheaecombinedwithenhancedluminescenceenzymeimmunoassaywereusedtotestforN.gonorrhea.Results:Aminimumof1×10^4/CFUofGCingenitaltractsecretionsorurinecouldbedetectedwiththetechniqueofluminescenceenzymeimmunoassay.Conclusion:TheenhancedluminescenceenzymeimmunoassayhastheadvantageofhighsensitivityandspecificityfordiagnosingNGfromgenitourinarytractsecretionandurine.

简介：AnewchemilurninescencelabelN-(β-carboxypropionyl)luminol(CPL)wasusedtolabelsheepanti-humanIgG(SaHIgG).Thelabeledantibodywasstableandcouldbedetectedatleastdownto10-17~10-16mol.Themolarincorporationratiowasestimatedtobe0.26molofCPLpermolofSaHIgG.TherewerenoapparentchangesintheimmunoreactivityofthelabeledSaHIgGandinthequantumefficiencyoftheCPLafterlabeling.

简介：Preparationandcharacterizationofthehapten-proteinconjugatesarefundamentaltodevelopingenvironmentalimmunoassays.Asahapten,1-pyrenebutyricacid(PBA)wasconjugatedtothecarrierproteinofbovineserumalbumin(BSA)orovalbumin(OVA)byactiveestermethod.Infraredspectra(IR)showedthatPBA-BSAandPBA-OVAconjugatesweresuccessfullyprepared.Thenumberofthehaptensconjugatedtothecarrierproteinwasdeterminedbyultravioletspectra(UV)andmatrix-assistedlaserdesorptionionizationtime-of-flightmassspectrometry(MALDI-TOF-MS).ThecalculatedaveragebindingratiosofPBA/BSAandPBA/OVAwere18:1and10:1byUV,and31:1and22:1byMALDI-TOF-MS,respectively.Althoughtherewasadiscrepancybetweentheresultsdeterminedbythetwomethods,bothofthemwereusefulforthecharacterizationofthehapten-proteinconjugates.TheantibodywasproducedagainsttheantigenofPBA-BSA,andtheaffinitywastestedbythedoubleagardiffusionmethod.Theconjugatesandtheantibodycouldbeusedfordevelopingasensitiveandselectiveimmunoassayofpolycyclicaromatichydrocarbons(PAHs).

简介：Inthispaper,itwasdiscoveredthatanovelpH-sensitivecopolymerofN-isopropylacrylamide(NIP)andN-(3-dimethylaminopropyl)methacrylamide(DMAPM)couldbegottenbypolymerization.ThephasetransitionpH(pHtr)ofP(NIP-DMAPM)polymerwasfoundtobe7.4at37℃.ThepolymerwasprecipitatedoutofwateraboveacriticalpH=7.4andre-dissolvedbelowpH----7.4.Thecharacteristicofthispolymermadeitpossibletocarryouttheimmunochemicalstepsofanimmunoassayinatruesolutionandthentoquicklyseparatetheresultingproductfromthereactionmixture.Inacompetitivefluorescenceimmunoassay,thestandardrabbitIgGandrabbitIgGimmobilizedonP(NIP-DMAPM)firstcompetitivelyreactedwiththefluoresceinisothiocyanate(FITC)labeledantibody,thenthepHofsolutionwasadjustedabovethepHtrofpolymertoprecipitatethepolymer-immunecomplex,andthepolymer-immunecomplexprecipitatewasseparatedandre-dissolvedbytheadjustmentofpH,finallytheFITC-labeledantibodyintheimmunecomplexwasquantifiedbyfluorescencemeasurement.ThecalibrationgraphforrabbitIgGwaslinearovertherangeof100-1000ng/mLwithadetectionlimitof11ng/mL.Themethodisrapid,sensitiveandsimple.OwingtoneutralpHtrofP(NIP-DMAPM),thedamagetoantigen-antibodyimmunecomplexwasgreatlydecreasedinthecourseofseparation.Inaddition,asandwichenzyme-linkedfluorescenceimmunoassaymethodforthedeterminationofhumanIgGwasalsodeveloped,showingthatthepH-sensitivephaseseparatingimmunoassaycouldbeperformedinthecompetitivemethodaswellasthesandwichmethod.

简介：摘要目的分析酶联免疫吸附试验（ELISA）和电化学发光技术（ECLIA）检测乙肝五项的结果差异。方法分别用ELISA法和ECLIA法测定107例乙肝病人血清标本的HBsAg、HBsAb、HBeAg、HBeAb、HBcAb，检测结果用阴性和阳性来表示，并用χ2检验进行统计学分析。结果两种方法对于HBsAg、HBsAb、HBeAg、HBeAb检测结果无统计学差异，对于HBcAb检测结果有显著性差异。结论ECLIA法检测灵敏度可能高于ELISA法，有条件的实验室应尽量采用ECLIA法检测乙肝五项。

简介：AbstractLike antibody evaluation, using an effective antigen-specific T-cell immunity assessment method in coronavirus disease 2019 (COVID-19) patients, survivors and vaccinees is crucial for understanding the immune persistence, prognosis assessment, and vaccine development for COVID-19. This study evaluated an empirically adjusted enzyme-linked immunospot assay for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T-cell immunity in 175 peripheral blood samples from COVID-19 convalescents and healthy individuals. Results of viral nucleic acid were used as the gold standard of infection confirmation. The SARS-CoV-2M peptide pool had higher sensitivity of 85% and specificity of 71% for the single peptide pool. For combined peptide pools, the parallel evaluation (at least one of the peptide pools is positive) of total peptide pools (S1&S2&M&N) had higher sensitivity (up to 93%), and the serial evaluation (all peptide pools are positive) of total peptide pools had higher specificity (up to 100%). The result of the serial evaluation was better than that of the parallel evaluation as a whole. The detection efficiency of M and N peptide pool serial evaluation appeared the highest, with a sensitivity of 80% and specificity of 93%. This T-cell immunity detection assay introduced in this report can achieve high operability and applicability. Therefore, it can be an effective SARS-CoV-2-specific cellular immune function evaluation method.

简介：【摘要】目的 探讨电化学发光免疫分析法(ECLIA)在梅毒检测中的应用效果。方法 梅毒患者血清标本51例和非梅毒患者血清样本91例，运用电化学发光免疫分析法和毒螺旋体抗体(ELISA)进行检测，对比敏感度、特异性和准确性。结果 电化学发光免疫与毒螺旋体抗体的敏感度为100％、98.04%，而特异性98.90%、100％，对比两方法差异无统计学意义(P>0.05)。ECLIA方法在敏感性方面高于ELISA方法。结论

简介：【摘要】目的 探讨分析对乙型肝炎病毒表面抗体采用电化学发光免疫技术进行定量检测的作用。方法 本次临床研究选取2018年12月到2020年12月期间在我院接受治疗的200例乙型肝炎病毒患者作为研究对象，全部患者均已得到确诊。对全部患者通过电化学发光免疫技术对乙型肝炎病毒表面抗体进行定量检测，获取对患者的检测结果，然后与确诊结果进行对比。结果 电化学发光免疫技术的检查结果与确诊结果对比，符合率无显著差异（P＞0.05）结论 根据本次研究的结果可以确认，通过电化学发光免疫技术对乙型肝炎病毒表面抗体进行定量检测的效果十分理想，不仅可以将其用于对患者的诊断工作，还可以为开展对患者的治疗工作提供重要的数据参考。

简介：【摘要】目的 分析电化学发光免疫分析技术(ECLIA)定量检测乙型肝炎病毒表面抗体(抗-HBs)的临床价值。方法 选取本院2020年04月-2021年04月间100例乙型肝炎病毒确诊患者作为观察对象，分为参照组（使用ELISA检测法）和研究组（使用ECLIA检测法），并病理检查结果为依据，比较应用效果。结果 研究组抗-HBs阳性检出率高于参照组（P＜0.05），研究组检查准确率接近于病理检查，差异无统计学意义（P＜0.05）。结论 ECLIA在检测抗-HBs中应用价值更高，其检出准确率近似于病理检查，在早期乙肝筛查及后续治疗中应用效果更加突出，具有推广价值。

简介：【摘要】目的：对比分析电化学发光法（ECLIA）与酶联免疫吸附法（ELISA）分别在乙型肝炎标志物检测中所发挥的价值。方法：分析检测的对象是确诊乙型肝炎病毒感染患者56例，其资料完整，对检测分析知情。分别对患者进行电化学发光法（ECLIA）检测（结果设定为研究组）和酶联免疫吸附法（ELISA）检测（结果设定为对照组），对比两组检测方式的准确率。结果：研究组的检测准确率明显高于对照组，组间数据对比P

简介：【摘要】目的 分析乙肝病毒感染不同免疫检验方法的效果。方法  双盲法随机抽取100例乙肝患者（2021年3月－11月），均进行ECLIA（观察组）、ELISA（对照组）检验，对比检验结果。结果 观察组检验准确率为98.00%高于对照组的77.00%（p=0.000）；观察组HBeAb、HBsAg、HBeAg、HBsAb、HBcAb阳性率为33.00%、88.00%、34.00%、22.00%、86.00%高于对照组的20.00%、71.00%、21.00%、19.00%、84.00%（p=0.037、0.002、0.039、0.599、0.692）。结论   ECLIA用于乙肝病毒感染血清学标志物检验中具有较高的价值。