简介：WedescribedtheconstructionofBACcontigsofthegenomeofaindicavarietyofOryzasativa.GuangLuAi4.Anentirerepresentative(Sixfoldcoverageofricechromosomes)andgeneticallystableBAClibraryofricegenomeconstructedinthislabhasbeensystematicallyanalysedbyrestrictionenzymefragmentationandpolyacrylamidegelelectrophoresis.Andalltheimagesthusobtainedweresubjecttoimage-processing,whichconsistedofpreliminarylocationofbands,cooperativetrackingoflanesbycorrelationofadjacentbads.aprecisedensitometricpass,alignmentatthemarkerbandswiththestandard,optionalinteractiveediting,andnormalizationoftheacceptedbands.ThecontigsweregeneratedbasedontheComputerSoftwarespeciallydesignedforgenomemapping.Thenumberofcontigswith600kbinlengthonaveragewas464.ofcontigswith1000kbinlengthonaveragewas107;ofcontigswith1500kbinlengthonaveragewasConstructionofOryzaSativagenomecontigs.23.Therefor,allthecontigswehaveobtainedampuntedupto420megabasesinlength.Consideringthesizeofricegenome(430megabased),thecontigsgeneratedinthislabhavecoverednearly98%ofthericegenome.Wearenowintheprocessofmappingthecontigstochromosomes.

简介：Asimplemethodtocreateachromosome-specificDNAlibrqaryofrice,includingmicrodissection,amplification,charterizationandcloning,isdescribed.Ricechromosome4fromametaphasecellhasbeenisolatedandamplifiedbytheLinkerAdapterPCR(LA-PCR).ThePCRproductswerelabeledasprobeswithDIG-11-dUTPusingtherandomprimingmethod.SouthernblotanalysiswithricegenomicDNAandspecificRFLPmarkersdemonstratedthatthePCRproductswerederivedfromricechromosome4.Alargelibrarycomprisingover100,000recombinantplasmidmicroclonesfromricechromosome4wasconstructed.Colonyhybridizationshowedthat58%oftheclonescontainedsingleorlow-copysequencesand42%containedrepetitivesequences.ThesizeofinsertsgeneratedbyPCRrangedfrom140bpto500bp.ThismethodwillfacilitatecloningofthespecificchromosomeDNAmarkersandimportantgenesofrice.

简介：Domaindatabaseisessentialfordomainpropertyresearch.Eliminatingredundantinformationindatabasequeryisveryimportantfordatabasequality.Herewereportthemanualconstructionofanon-redundanthumanSH2domaindatabase.Thereare119humanSH2domainsin110SH2-containingproteins.HumanSH2swerealignedwithClustalX,andahomologoustreewasgenerated.Inthistree,proteinswithsimilarknownfunctionwereclassifiedintothesamegroup.Someproteinsinthesamegrouphavebeenreportedtohavesimilarbindingmotifsexperimentally.Thetreemightprovidecluesaboutpossiblefunctionsofhypotheticalproteinsforfurtherexperimentalverification.

简介：Copperandironplayimportantrolesinavarietyofbiologicalprocesses,especiallywhenbeingchelatedwithproteins.Theproteinsinvolvedinthemetalbinding,transportingandmetabolismhavearousedmuchinterest.Tofacilitatethestudyonthistopic,weconstructedtwodatabases(DCCPandDICP)containingtheknowncopper-andiron-chelatingproteins,whicharefreelyavailablefromthewebsitehttp:∥sdbi.sdut.edu.cn/en.Userscanconvenientlysearchandbrowsealloftheentriesinthedatabases.Basedonthetwodatabases,bioinformaticanalyseswereperformed,whichprovidedsomenovelinsightsintometalloproteins.

简介：吉本斯们在进化期间经历了广泛的karyotype重新整理并且为学习进化chromosomal重新整理的内在的分子的机制代表一个理想的模型。克隆和进化chromosomal断点的顺序描述将提供重要卓见进驾驶了如此的激进的karyotype的分子的力量，这被期望在长臂猿改组。我们构造了并且描绘非包含192,000的白脸颊的长臂猿(Nomascusleucogenys)的一个高质量的fosmid图书馆--有38kb和2.5褶层染色体范围的一种平均insert尺寸的冗余的克隆。到100随机选择的fosmid克隆定序的结束，我们为图书馆产生了196个顺序标签。这些定序结束的fosmid克隆然后被荧光在situ杂交印射到白脸颊的长臂猿的染色体上，并且没有假妄想的克隆被检测。对人的染色体的强风搜索显示出在点击克隆的数字和染色体的数字之间的好关联，fosmid图书馆的不偏的chromosomal分发的一个指示。印射的克隆的chromosomal分发与对人、白脸颊的长臂猿染色体的BLAST搜索结果也一致。fosmid图书馆和印射的克隆愿望在更小的无尾猿为进一步学习长臂猿的chromosomal重新整理和内在的分子的机制以及为比较genomic学习用作一个珍贵资源。

简介：Thec-erbB-2proto-oncogeneencodesa185kDaproteinp185,whichbelongstoepidermalgrowthfactorreceptorfamily.Amplificationofthisgenehasbeenshowntocorrelatewithpoorclinicalprognosisforcertaincancerpatients.ThemonoclonalantibodyA21whichdirectedagainstp185specificallyinhibitsproliferationoftumorcellsoverexpressingp185,henceallowsittobeacandidatefortargetedtherapy.InordertoovercomeseveraldrawbacksofmurineMAb,wecloneditsVHandVLgenesandconstructedthesingle-chainFv(scFv)throughapeptidelinker.TherecombinantscFvA21wasexpressedinEscherichiacoliandpurifiedbytheaffinitycolumn.SubsequentlyitwascharacterizedbyELISA,Westernblot,cellimmunohistochemistryandFACS.Alltheseassaysshowedthebindingactivitytoextracellulardomain(ECD)ofp185.BasedonthosepropertiesofscFvA21,wefurtherconstructedthescFv-Fcfusionmoleculewithahomodimerformandtherecombinantproductwasexpressedinmammaliancells.Inaseriesofsubsequentanalysisthisfusionproteinshowedidenticalantigenbindingsiteandactivitywiththeparentantibody.Theseanti-p185engineeredantibodieshavepromisedtobefurthermodifiedasatumortargetingdrugs,withaviewofapplicationinthediagnosisandtreatmentofhumanbreastcancer.