简介：Objective:ToconstructsurvivinshRNAexpressionvectorcartingenhancedgreenfluorescentproteingene,transfectitintoGBC-SDHcellsviaelectroporation,andgetGBC-SDcellswhicharestableexpressingsurvivinshRNA.Methods:ThesiRNAsequencetargetingsurvivinmRNAwassynthesizedandclonedintopEGFP-H1.TheconstructedplasmidandpEGFP-H1weretransfectedintoGBC-SDcellsrespectivelyvialiposome,andthetransfectingeffectwasdetectedwithFlowCytometry.ThenthetransfectedcellswereselectedwithG418.Results:Therecombinantplasmidwassuccessfullyconstructed,namedpEGFP-survivin.ThegenetransfectionefficienciesinpEGFP-H1-transfectedgroupandpEGFP-survivin-transfectedgroupwerethe80.29%±2.71%and83.85%±2.34%(P>0.05),whichwassuccessfultogetthecellsthatarestableexpressingshRNA,namedGBC-SD/EGFPandGBC-SD/survivin.Conclusion:SurvivinshRNAexpressionvectorwasconstructedsuccessfullyandgotGBC-SDcellswhicharestableexpressionshRNA.

简介：Theeffectsofvariouscartilageextracellularmatrixontheconstructionofrabbitgrowthplatecartilagetissueinvitrowerestudied.Theresultsshowthatcollagen,proteoglycanandhyaluronicacidcanpromotethegrowthofculturedchondrocytesbuttheeffectsofvariouscartilageextracellularmatrix(ECM)onchondrocytedifferentiationaredifferent.Collagencanpromotethehypertrophyofchondrocyteswhileproteoglycanandhyaluronicacidinhibitthetransitionofmaturechondrocytesintohypertrophiedchondrocytes.

简介：INTRODUCTIONThecommonconstructionmethodoftissueengineeredarticularcartilageistoisolateandculturechondrocytes,transplantthemintothedegradablescaffoldmaterials,thentorepairthedefectofarticularcartilage.Wedesignedthisexperimenttoinvestigatethefeasibilityoftissueengineeredarticularcartilageconstructedbythetechniqueofcentrifugetubeculturewithoutscaffoldmaterials.

简介：Theresearchgroupwassuccessfulingeneratingavasculargraftwithbiomimeticcircumferentialtensilestrengthandexpressionofsmoothmusclecellspecificgenesoverstaticculture.

简介：Thisresearchusesalginateandhyaluronicacidasthemaincomponenttopreparesupport,thenexploresthepossibilitiesasatissueengineeringscaffold.Firstly,prepareHAwithvariousaveragemolecularweightandalginatewithdifferentviscosity,mixthemupatacertainproportionandmakeitintoaAlgCa2+-HAcompositescaffoldwithafilm-formingmethod.Thisarticlediscussesthefeasibilityofthisscaffoldusedintissueengineeringfieldaccordingtotheconsequenceofmoisturecontenttesting,mechanicalanalysis,andscanningelectronmicroscopyanalysis.ThestructureandpropertiesofAlgCa2+-HAcompositescaffoldarecloselyrelatedtosomefactorssuchasaveragemolecularweightofhyaluronicacid,hyaluronicacidconcentration,alginateviscosity,cross-linkingagentsandprocessingtechnology.TheAlgCa2+-HAcompositematerial,whichisatdifferentproportionsandaddingdifferentcross-linkingagent,hassomecertaincharacteristics:moisturecontentrangingfrom50%to95%,tensilestrengthbetween2.69N/mm2and4.299N/mm2,andelongationatbreakisabout58%to160%.ThepreparedAlgCa2+-HAcompositescaffoldscanbeusedastissueengineeringscaffoldsresultingfromitshighmoisturecontent,goodmechanicalpropertiesandidealporestructure.更多还原

简介：INTRODUCTIONApproximately400millionpersonsworldwidesufferfrombladderdisease.Individualswithend-stagebladderdiseaseoftenrequirebladderreplacementorrepair.Severalbladdersubstituteshavebeenattemptedwithbothorganicmaterialsandsynthetics.

简介：IntroductionAsamemberofthebonemorphogeneticprotein(BMP)family,BMP-2playsimportantrolesnotonlyinboneregenerationandbonerepairbutalsoincellproliferation,apoptosis,differentiationandmorphogenesis.TheBMP-2remarkableabilitytostimulatenewbonegrowthresultsinthedevelopmentofanoveltherapystrategyforbonemassdefectduetoaccidentsordiseases.BecausetheBMP-2itself,inconjunctionwithasuitablematrix,issufficienttostimulategenesisofnewbone,thegeneticallyengineeredBMP-2hasgoodappliedprospects.

简介：Prematureventricularcontraction(PVC)isthemostfrequentarrhythmiaencounteredinclinicalpractice.PVCmayoccurinhealthsubjects,whichisnotimminentlylife-threateningbutmayrequiretherapiestopreventfurtherproblems.So,thetimelyPVCrecognitionbecomesveryimportantfortheanalysisofelectrocardiogram(ECG),especiallyfortheremoteECGmonitoringusingmobilephones.Inthispaper,aconstructionmethodofpersonalizedECGtemplateandaPVCrecognitionmethodbasedontemplatematchingwerestudied.Firstly,weselected43ECGrecordingsfromtheMIT-BIHarrhythmiadatabase.Allrecordingsweredividedintotwodatasets(DS1fortrainingandDS2fortesting)andeachdatasetapproximatelycontainedthesameproportionofPVCbeats.Subsequently,foreachrecording(30min)inDS1,thefirst5minrecordingswereusedtoconstructthepersonalizedECGtemplateandthelast25minrecordingswereusedfortheR-wavepeaksdetectionandPVCrecognition,wherethetemplatematchingmethodwereused.ThevalidityoftheproposedmethodswastestedusingDS2.Theresultsshowedthat:1)highbeatdetectionaccuracywasachievedforbothPVCbeatsandnon-PVCbeats;2)thesensitivityandspecificityofPVCrecognitionwere99.11%and99.96%forthefirst5minrecordingsrespectively,99.17%and99.43%forthelast25minrecordingsrespectively.Alltheproposedmethodscanbereal-timeperformed,whichshowapromisingprospectfortheapplicationofECGmobilephones.

简介：客观：击倒链球菌mutans(S.mutans)的全部勒克司基因经由相应再结合和构造的UA159紧张S的删除勒克司的变异的紧张。Mutans。学习S的酸抵抗之间的差别。MutansIngbrittC国际标准紧张和勒克司变异的紧张的酸抵抗。方法：二DNA碎裂定位在勒克司上面、下游基因被放大，在他们之间的PJT10的抗生素的一种抵抗基因被设计进PUC19为构造再结合的plasmidplasmidpUCluxKO。S.mutans房间与的ElectrotransformationpUCluxKO变异导致了抗生素的一种的隔离抵抗S。Mutanstransformants，它被聚合酶链反应，V.harveyiBB170光生物鉴定和定序的分析识别。S的答案。有一样的密度的Mutans标准紧张和勒克司变异的紧张被做并且在pH有教养为一样的period.Terminal生长状况的3.5～7.0BHI液体compared.Firstly在pH被酸化5.5BHI液体，二紧张在pH是有教养的3.0BHI液体。二紧张的酸忍耐回答是compared.Results:限制endonuclease分析证明那pUCluxKO变异的向量成功地被重新结合。S.mutans异种的删除勒克司的地位被PCR与为勒克司和抗生素的一种抵抗的基因特定的教材证实。S.mutans异种不能导致生物体之发光，indiating异种成功地被重新结合。在文化的二十代以后，构造中国S.mutans异种被证实稳定。aciduricity的重要差别在S.mutans标准紧张和标准紧张的抵抗是的勒克司变异的strain.The酸之间被观察比二紧张两个都显示了的勒克司变异的strain.The的强壮酸忍耐回答的能力。结论:S.mutans基因突变而产生之遗传的交换plasmid校正地被构造并且一S.mutans的勒克司否定的异种被构造，它能帮助推进在S.mutans的致病学习勒克司的角色。勒克司变异的紧张对酸忍耐回答的酸inactivation，而是能力更敏感仍然存在。