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简介：CirculatingTumorCells(CTC)havethepotentialtobeusedclinicallyasadiagnostictoolandatreatmenttoolinthefieldofoncology.Asadiagnostictool,CTCmaybeusedtoindicatethepresenceofatumorbeforeitislargeenoughtocausenoticeablesymptoms.Asatreatmenttool,CTCisolatedfrompatientsmaybeusedtotesttheefficacyofchemotherapyoptionstopersonalizepatienttreatment.Onewayfortumorstospreadisthroughmetastasisviathecirculatorysystem.CTCareabletoexploitthenaturalleukocyterecruitmentprocessthatisinitiallymediatedbyrollingontransientselectinbonds.OurcapturedevicestakeadvantageofthisnaturallyoccurringrecruitmentsteptoisolateCTCfromwholebloodbyflowingsamplesthroughselectinandantibody-coatedmicrotubes.Wholebloodwasspikedwithaknownconcentrationoflabeledcancercellsandthenperfusedthroughpre-coatedmicrotubes.Microtubeswerethenrinsedtoremoveunboundcellsandthenumberoflabeledcellscapturedonthelumenwasassessed.CTCweresuccessfullycapturedfromwholebloodataclinicallyrelevantlevelontheorderof10cellspermL.Combinationtubeswithselectinandantibodycoatedsurfaceexhibitedhighercaptureratethantubescoatedwithselectinaloneorantibodyalone.Additionally,CTCcapturewasdemonstratedwiththeKG1ahematopoieticcelllineandtheDU145epithelialcellline.Thus,theinvivoprocessofselectin-mediatedCTCrecruitmenttodistantvesselwallscanbeusedinvitrototargetCTCtoatubelumen.ThebiomolecularcoatingscanalsobeusedtocaptureCTCofhematopoieticandepithelialtumororiginandisdemonstratedtosensitivitiesdowntotheorderof10CTCpermL.Inarelatedstudyaimedatreducingthebloodbornemetastaticcancerload,wehaveshownthatcellscapturedtoasurfacecanbeneutralizedbyareceptor-mediatedbiochemicalsignal.IntheproposedmethodwehaveshownthatusingacombinedselectinandTRAIL(TNFRelatedApoptosisInducingLigandorApo2L)functionalizedsurfaceweareabl

简介：Amathematicalmodelforatmosphericstagedcirculatingfluidizedbedcombustion,whichtakesfluiddynamics,combustion,heattransfer,pollutantsformationandretention,intoaccountwasdevelopedintheinstituteofEngineeringThermophysics(IET)recently.Themodelofgassolidflowatthebottomofthecombustorwastreatedbythetwo-phasetheoryoffluidizedbedandintheupperregionasacore-annulusflowstructure.ThechemicalspeciesCO,CO2,H2,H2O,CH4,O2andN2wereconsideredinthereactionprocess.Themathematicalmodelconsistedofsub-modelesoffluidnamics,coalheterogeneousandgashomogeneouschemicalreactions.heattransfer,particlefragmentationandattrition,massandenergybalancetec.Thedevelopedcodewasappliedtosimulateanoperatingstagedcirculatingfluidizedbedcombustionboilerofearlydesignandtheresultswereingoodagreementwiththeoperatingdata.Themainsubmodelsandsimulationresultsaregiveninthispaoper.

简介：Circulatingtumorcells(CTCs)areapopulationoftumorcellsmediatingmetastasis,whichresultsinmostofthecancerrelateddeaths.ThenumberofCTCsintheperipheralbloodofpatientsisrare,andmanyplatformshavebeenlaunchedfordetectionandenrichmentofCTCs.EnumerationofCTCshasalreadybeenusedasaprognosismarkerpredictingthesurvivalrateofcancerpatients.YetCTCsshouldbemorepotential.StudiesonCTCsatsinglecelllevelmayhelprevealingtheunderlyingmechanismoftumorigenesisandmetastasis.Thoughfarfromdeveloped,thisareaofstudyholdsmuchpromiseinprovidingnewclinicalapplicationanddeepunderstandingtowardsmetastasisandcancerdevelopment.

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简介：Fromthebeginningof1970’s,theresearchworkonstrandcurrentdistributioninhydro-generatorstatorbarshasbeenmademarkedprogressinChina.Inordertoknowwhythegeneratorbarinsulationswerebrokendownsooftenbothinop-erationandintestingafterthegeneratorsbeingputintooperationfor8-10yearsatXinanjiangHydropowerStation.Investigationswereconductedandtheresultsoffieldmeasurementshowedthatthe

简介：Circulatingtumorcells(CTCs)arethecancercellsthatcirculateintheperipheralbloodafterescapingfromtheoriginalormetastatictumors.CTCscouldbeusedasnon-invasivesourceofclinicalinformationinearlydiagnosisofcancerandevaluationofcancerdevelopment.Inrecentyears,CTCresearchhasbecomeahotspotfieldwhereinmanynovelCTCdetectiontechnologiesbasedonmicrofluidicshavebeendeveloped.Greatadvanceshavebeenmadethatexhibitobvioustechnicaladvantages,butcannotyetsatisfythecurrentclinicalrequirements.Inthisstudy,wereviewthemainadvancesinisolationanddetectionmethodsofCTCbasedonmicrofluidicsresearchoverseveralyears,proposefivetechnicalindicatorsforevaluatingthesemethods,andexploretheapplicationprospects.Wealsodiscusstheconcepts,issues,approaches,advantages,limitations,andchallengeswithanaimofstimulatingabroaderinterestindevelopingmicrofluidics-basedCTCdetectiontechnology.

简介：Ovariancancerisoneofthemostlethalmalignantgynecologicaltumors.Morethan70%ofpatientswithovariancancerarediagnosedatadvancedstage.The5-yearsurvivalinpatientswithadvancedovariancancerislessthan30%becauseofthelackofeffectivebiomarkersfordiagnosis,prognosis,andpersonalizedtreatment.MicroRNA(miR)isaclassofsmallnoncodingRNAsthatnegativelyregulategeneexpressionprimarilythroughpost-transcriptionalrepression.ManystudiesontissuemiRinovariancancerhavebeencarriedoutandshowgreatpotentialinclinicalpractice.However,tissuesamplesarenoteasilyavailablebecausesamplingcausesinjury.Researchershavestartedtofocusonplasma/serummiR,assumingthatbloodsamplesmayreplacetissuesamplesinmiRresearchinthefuture.Plasma/serummiRresearchisstillinitsearlystages.Studiesonitsfunctionintheearlydiagnosisofovariancancerhaveachievedsomeprogress,butplasma/serummiRprofilingforprognosisandpersonalizedtreatmentofovariancancerremainsunknown.Athoroughunderstandingofthefunctionofplasma/serummiRinovariancancerwillfacilitateearlydiagnosisandimprovetreatmentforovariancancer.

简介：Gas-solidtwo-phaseflowinacirculatingfluidizedbed(CFB)isaffectedbyoperatingconditions(e.g.,superficialgasvelocity,solidsinventory),materialpropertiesandgeometricfactors,suchastheentryandexitconfiguration.Inparticular,thesuspensionsection,whichislocatedbetweentheriserbottomandthesolidsrecycleinlet,affectsthehydrodynamicsintherisersignificantly.However,thesuspensionsectionhasreceivedlessattentioncomparedwithothergeometricfactors.Mostcomputationalfluiddynamics(CFD)simulations,especiallytwo-dimensionalsimulationsdonottakethisfactorintoaccount.Weperformedthree-dimensional,full-loopCFDsimulationswithadragcoefficientthatwasdeterminedbytheenergy-minimizationmulti-scalemodel,andinvestigatedtheflowbehavioroftwoCFBswithdifferentsuspension-sectionlengths.ThesimulationresuitsrevealedthattheaxialprofilesofvoidageintheriserwithalongersuspensionsectionaremorelikelyS-shaped,whereasthosewithshortersuspensionsectionsdecayexponentially.ThedependencesofsolidsfluxonsolidsinventorydifferinthetwoCFBs.Ashortersuspensionsectionmayresultinasmoothtransitionfromdilutetodensetransportwithoutintermediateaccumulativechoki-ng,whereasaIongersuspensionsectionmayleadtoachokingtransition.Thesesimulationresultsarequalitativelyconsistentwiththeflowbehaviorsdescribedinliterature.

简介：Withtherevisionofemissionstandards,deepdesulphurizationandDeNOXisneededincirculatingfluidizedbed(CFB)boilers.Theoperationofthefirstsetof300-MWCFBboilerpluslimestone/gypsumwetfluegasdesulphurization(FGD)systemintheworldshowsthatdeepdesulphurizationandDeNOXofCFBboilershashigherSO2removalefficiencyatalowerCa/Sratiocomparedwithtraditionalinnerdesulphurizationmode.Itcanmeettheincreasinglyrigidemissionstandards,andissuitableformorefuels.DeepdesulphurizationandDeNOXcanalsoachieveahighly-efficienthigh-temperatureCFBboilerthatcannotonlyachieveinnerdesulphurizationandlowNOXemission,butbenefitslow-grade,highsulfurcontentfuelsaswell.ResearchofdeepdesulphurizationandDeNOXwillbeadevelopingdirectionforCFBboilers.

简介：AbstractObjective:Laboratory diagnosis of neurosyphilis (NS) remains a great challenge. This study was the aimed to identify miRNA candidates as biomarkers to distinguish between NS, non-neurosyphilis, and healthy controls (HCs).Methods:We analyzed miRNA expression profiles in peripheral blood mononuclear cells (PBMCs) from six patients with NS, eight patients with secondary syphilis (SS), and five HCs using microarray technology. The differentially expressed miRNAs were validated in 33 NS samples, 31 SS samples, and 30 HC samples using TaqMan miRNA real-time qPCR (qRT-PCR).Results:Thirty-nine miRNAs were differentially expressed in SS and NS patients compared with HCs. Thirteen miRNAs were randomly selected to validate their expression levels in the same samples used in microarray assay by qRT-PCR. All miRNAs were upregulated in SS and NS samples compared with HC. qRT-PCR analysis of the expression of the 13 miRNAs in a second cohort (76 samples) showed that the average expression levels of nine miRNAs were higher in SS than in NS (SS: 0.185, NS: 0.136, P=3.8E-10), while the expressions of the other four miRNAs were lower in SS than in NS (SS: 0.000757, NS: 0.000873, P=0.022). ROC curve analysis of the 13 miRNAs showed the area under the curve value to be 1.00 for distinguishing SS patients from HCs, 1.00 for distinguishing NS patients from HCs, 1.00 for distinguishing SS and NS patients from HCs, and 0.968 for distinguishing NS from SS patients.Conclusion:The present study is the first one that identified differentially expressed miRNAs in PBMCs from patients with NS. Our results suggest that the 13 candidate miRNAs in PBMCs may be novel noninvasive biomarkers for NS diagnosis.

简介：BackgroundStudieshaveshownthatmiRNA-155playedanimportantroleintheprocessofdevelopmentofhypertension.However,thereisnodateaboutmiRNA-155andbloodpressuremonitoringparameters.Therefore,weexaminedwhetherinhypertensivepatientstheexpressionlevelofplasmamiRNA-155relatedto24-hambulatorybloodpressuremonitoring(ABPM)parameters.MethodsAcohortofadultpatientsscheduledtoreceivephysicalexamination,officeandambulatorybloodpressuremonitoring.Quantitativereversetranscriptasepolymerasechainreaction(qRT-PCR)wasusedtoevaluatetheexpressionofselectedmiRNA-155.ThemiRNA-155expressionlevelcorrelationbetweenbloodpressureparameterswasassessedusingtheSpearmancorrelationcoefficient.ResultsFiftyfouressentialhypertensionpatients(25men;meanage,53.28±9.52years)andthirtyhealthyvolunteers(15men;meanage,53.03±5.87years)wereincluded.WeobservedhigherexpressionlevelofmiRNA-155(32.31±2.85vs27.21±1.59,P<0.001)inhypertensivepatientscomparedtohealthycontrolindividuals.MiRNA-155expressionlevelshowedsignificantpositivecorrelationwith24hDaytimeSBP(r=0.681,P<0.001),24hDaytimeDBP(r=0.473,P<0.001),24hDaytimePP(r=0.565,P<0.001)anddipping(r=0.257,P=0.018),respectively.ConclusionsOurstudyshowedthatmiRNA-155expressionlevelwasassociatedpositivelywithdaytimebloodpressuremonitoringparameters,aswellasbloodpressurevariability,indicatingapossibleimplicationofmiRNA-155inthepathogenesisofhypertension.

简介：Inthepresentwork,artificialneuralnetworks(ANNs)wereproposedtomodelnonlineardynamicbehaviorsoflocalvoidagefluctuationsinducedbyhighlyturbulentinteractionsbetweenthegasandsolidphasesincirculatingfluidizedbeds.Thefluctuationsoflocalvoidageweremeasuredbyusinganopticaltransmittanceprobeatvariousaxialandradialpositionsinacirculatingfluidizedbedwithariserof0.10mininnerdiameterand10minheight.TheANNstrainedwithexperimentaltimeserieswereappliedtomakeshort-termandlong-termpredictionsofdynamiccharacteristicsinthecirculatingfluidizedbed.Anearlystopapproachwasadoptedtoenhancethelong-termpredictioncapabilityofANNs.TheperformanceofthetrainedANNwasevaluatedintermsoftime-averagedcharacteristics,powerspectra,cyclenumberandshort-termpredictabilityanalysisoftimeseriesmeasuredandpredictedbythemodel.

简介：Inthispaper,multifractalanalysistogetherwithwavelettransformmodulusmaxima(WTMM)methodisusedtoanalyzethefluctuatingsignalsofcirculatingfluidizedbed(CFB).Singularityspectrumshowsthatthegas-particleflowinCFBhasmultifractalcharacter.Motionbehavioroftheparticle-fluidsystemofCFBcanbedescribedbysingularityspectrum.Intermittencyindexcanbeusedtodeterminethetransitionofflowregimefromfastfluidizationtopneumaticconveying.

简介：Exosomes是endocytic起源的nanoparticles，由由他们调制cell-to-cell通讯的能力的优点正在吸引增加的注意的无数房间人口藏匿了。他们也在许多免疫学的问题正在吸引注意，包括autoimmunity和，特别地调整cytokine和chemokine激活的他们的能力。主要胆汁的肝硬化(PBC)被认为一个模型自体免疫疾病，它对胆汁的上皮的房间有高度集中的细胞毒素的回答。我们与PBC和30健康控制(HC)从29个病人从血浆孤立exosomes，并且用一个前vivo系统在mononuclear房间人口在co-stimulatory分子表示和cytokine生产上学习了这些exosomes的效果。我们也与HCexosomes相比在PBC识别了microRNA(miRNA)人口。我们此处报导尽管exosomes不改变cytokine生产，他们显著地确实在介绍抗原的人口上改变co-stimulatory分子表示。进一步，我们在CD14+单核白血球上表明了那CD86起来调整的表情，而在CD11c+上起来调整的CD40由从有PBC的病人的exosomes的树枝状的房间。另外，有在有PBC的病人的传播exosomes的miRNA表示的差别。这些数据基于co-stimulatory分子玩的观察有重要重要性在T房间激活的规定的一个微分角色。我们的观察显示从PBC的异常exosomes有选择地在介绍抗原的房间的不同子集导致co-stimulatory分子的表示。这些改变可以在自体免疫的肝疾病的致病包含。

简介：变形乳癌(MBC)被肿瘤生长，增长和变形前进的联合描绘并且典型地与辩解的目的被管理。标准全身的治疗的利益是相对有限的，疾病被认为医不好建议需要调查变形过程的各种各样的阶段的生物司机以便改进分子地驾驶的治疗的选择。察觉，枚举和传播肿瘤房间(CTC)的分子的分析提供一个吸引人的机会推进这知识。食物和药变清管理的CellSearch系统枚举的CTC在MBC病人是没有前进的幸存(PFS)和全面幸存(OS)的一个独立预示的因素。几份出版报纸为介绍了在血的7.5mL的基础CTC计数5的MBC病人表明了差的预后。因此，CTC的枚举在为MBC的治疗期间向一个工具提供比用常规放射学的测试的解剖评价的标准预定早预言疾病的前进的能力。在词法的变形过程癌症房间展览和phenotypic粘性经历epithelialmesenchymal转变(EMT)期间。这重要现象与被联系在上皮的标记的规定下面(例如，EpCAM)与在当前的CTC丰富方法的适用性的潜在的限制。在很多调查翻译的如此的观察瞄准了改进我们的能力枚举并且表现CTC的分子的描述。理论上，CTC的phenotypic分析能代表能对变形疾病识别一个新潜在的目标并且推进发展并且个性化的治疗监视的胸肿瘤的液体活体检视。

简介：BackgroundAcutecoronarysyndrome(ACS)isaleadingcauseofmortalityandmorbidityworldwide,whichcomprisesunstableangina(UA)andacutemyocardialinfarction(AMI),andtheinvestigationofbiologicalmarkerstoassessthosemostatriskofrecurrentcardiovasculareventsisnecessary.MethodsSixty-sixhealthycontrolpeopleand67casesofUApatientswereenrolledinGuangdonggeneralhospital.Enzymelinkedimmunosorbentassay(ELISA)wasusedtodetectthelevelofplasmaACTG2.Thereceiveroperatingcharacteristiccurve(ROC)wasusedtoanalyzethepredictionvalueofACTG2forUA.AccordingtotheaveragelevelofplasmaACTG2inUApatients,UApatientsweredividedintothelowACTG2group(

简介：在传播的Gassolid流动使流体化床(CFB)起床人操作条件取决于不仅而且在出口配置上。很少研究用计算液体动力学(CFD)在流动结构上调查了出口配置的效果。这份报纸提供2D与光滑、T突然的出口模仿一个冷凳子规模平方剖面图起床人的二液体的模型。在煤气、稳固的阶段之间的drag力量在CFD起一个重要作用。因为drag力量模型例如Wen-Yu关联，不能在gassolid流动基于同类的二阶段的流动捕获异构的结构，结构依赖者精力最小化多尺度(扩展内存管理程序)拖力量模型(王，Ge，与李，2008)，为GeldartB粒子(沙)适用，集成于二液体的模型。计算轴的固体停顿侧面为T突然的出口是为光滑的出口和塑造C的曲线的分别地指数的曲线，与试验性的数据一致的两个。这研究再次证明关键角色在CFD模拟并且也拖力量显示CFD模拟(二液体的模型)的有效性在CFB起床人在gassolid流动上描述出口效果。

简介：Thecombustionperformanceoftheboilerlargelydependsonthecoaltype.Lotsofexperimentalresearchshowsthatdifferentfuelshavedifferentcombustioncharacteristics.ItisobviousthatfuelwillchangethewholeoperatingperformanceofCirculatingFluidizedBedCombustion(CFBC).Weknoweveninapilot-scalerunningboiler,themeasurementofsomeparametersisdifficultandcostly.Therefore,wedevelopedthewayofsimulationtoevaluatethecombustionperformanceofChinesecoalsinCFB,Thesimulationresultsshowthat,differentcoalswillresultindifferentcoalparticlediameterandcominutiondependingontheirmineralcomponentandthechangewillaffectthedistributionofashinCFBCsystem.Inaword.thecomputationalresultsareinaccordancewithexperimentalresultsqualitativelybuttherearesomedifferencesquantitatively.