简介:目的研究促癌的miR-21在腹水来源的高转移人肝癌细胞SK-HEP-1的表达及对其生物学行为的影响.方法利用qRT-PCR检测miR-21在SK-HEP-1细胞中的表达.分别利用细胞活力和细胞毒性检测法(cellcountingkit-8,CCK-8法)、流式细胞仪及transwell小室评估miR-21对SK-HEP-1细胞增殖、凋亡及侵袭能力的影响.结果相比于肝正常组织,miR-21高表达于SK-HEP-1细胞(>5倍).抑制其高表达后,SK-HEP-1细胞的增殖和迁移能力下降,但凋亡无明显变化.结论miR-21高表达于SK-HEP-1细胞,并能促进其增殖及侵袭能力.抑制其表达可能成为治疗肝癌的新途径.
简介:TheControlRoomLogbook(CRL)isdesignedtoimproveandreplacethepaperlogbookstraditionallyusedintheHEPacceleratorcontrolroom.Itsfeaturesbenefittheon-linecoordinator,theshiftoperators,andtheremoteobservers,Thispaperexplainssomeofthemostattractivefeaturesforeachoftheseroles.Thefeaturesincludetheabilitytoconfigurethelogbookforthespecificneedsofacollaboration,alargevarietyofentrytypesoperatorcanaddbysimplyclickinganddragging,andaflexiblewebinterfacefortheremoteobservertokeepupwithcontrolroomactivities.TheentriesaresavedasUTF-8basedXMLfiles,whichallowedustogivethedatastructureandmeaningsuchthatitcaneasilybeparsedinthepresentandfarintothefuture.TheXMLtagdataisalsoindexedinarelationaldatabase,makingqueriesondates,keyworks,entrytypeandothercriteriafeasibleandfast.TheCRLisusedintheD0controlroom.Thispresentationalsodiscussesourexperiencewithdeployment,platformindependenceandotherinterestingissuesthatarosewiththeinstallationanduseoflogbook.
简介:系统分析了KJ—1记录器对拾震器输出电压灵敏度的要求,并对SK拾震器与KJ—1记录器的连接方法进行了研究。研究结果表明,只需对SK拾震器和KJ—1记录器进行改造,就能实现两者的完美配接,而其性能优于DK—1地震仪和基式地震仪。
简介:Preliminaryobservationsontheresultofinfectionbyparvovirusofprimaryculturedcellsofhumanhepatomaandparahepatomatissuewerereported.Humanhepatomaandparahepatomatissuewereobtainedbytheculturemethodoftissuefragmentplating.ItwasobservedthattheirrespectivesensitivitytoparvovirusH-1wasdifferent.H-1hadinhibitoryeffectonepithelialcellsofhepatomatissuebutwithoutanysignificanteffectonfibroblastssimultaneously,H-lhadnoeffectonepithelialcellsandfibroblastsofparahepatomatissue.Furtherinvestigationoftherelationshipbetweenparvovirusandhepatomawouldbehelpfulnotonlyinelucidatingthemechanismofcarcinogenesisanditssuppressionbutalsointhesearchofnewapproachforthetreatmentofhepatoma.
简介:WepresentaninitialstudyoftheobjectfeaturesofOracle9i-thefirstofthemarket-leadingobject-relationaldatabasesystemsthatsupportsatrueobjectmodelontheserversideaswellasanODMG-styleC++languagebindingontheclientside.WediscusshowthesefeaturescanbeusedtoprovidepersistentobjectstorageintheHEPenvironment.