简介：AIM:Toinvestigatewhetherphotoreceptornecroptosisinducedbyz-VAD-FMK(pancaspaseinhibitor)wasinvolvedtheactivationofautophagyandwhetherNecrostatin-1,aspecificnecroptosisinhibitor,couldinhibitthisinductionofautophagyafterexperimentalretinaldetachment.METHODS:ExperimentalretinaldetachmentmodelswerecreatedinSprague-Dawleyratsbysubretinalinjectionofsodiumhyaluronateandsubretinalinjectionsofz-VAD-FMK,vehicleorz-VAD-FMKplusNecrostatin-1.Threedaysafterretinaldetachment,morphologicchangeswereobservedbytransmissionelectronmicroscopy.Inotheranimals,retinasweresubjectedtoimmunoprecipitationandWesternBlotting,thenprobedwithanti-RIP1,phosphoserine,LC-3IIorcaspase8antibody.RESULTS:Itwasprovedbyimmunoprecipitationandwesternblotting,thatphotoreceptornecroptosiswasmediatedbycaspase-8inhibitionandreceptorinteractingproteinkinase(RIP1)phosphorylationactivation.TransmissionelectronmicroscopeandwesternblottingresultsindicatedthatphotoreceptornecroptosiswasinvolvedtheLC-3IIandautophagosomesinduction.WealsodiscoveredNecrostatin-1couldinhibitRIP1phosphorylationandLC-3IIinduction.CONCLUSION:Thesedatafirstlyindicatephotoreceptornecroptosisisassociatedwiththeactivationofautophagy.Necrostatin-1protectsphotoreceptorsfromnecroptosisandautophagybydown-regulationofRIP1phosphorylationandLC-3II.
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