简介：Thelocalthermalconductivityofpolycrystallinealuminumnitride(AlN)ceramicsismeasuredandimagedbyusingascanningthermalmicroscope(SThM)andcomplementaryscanningelectronmicroscope(SEM)basedtechniquesatroomtemperature.ThequantitativethermalconductivityfortheAlNsampleisgainedbyusingaSThMwithaspatialresolutionofsub-micrometerscalethroughusingthe3ωmethod.Athermalconductivityof308W/m·Kwithingrainscorrespondingtothatofhigh-puritysinglecrystalAlNisobtained.Theslightdifferencesinthermalconductionbetweentheadjacentgrainsarefoundtoresultfromcrystallographicmisorientations,asdemonstratedintheelectronbackscattereddiffraction.Amuchlowerthermalconductivityatthegrainboundaryisduetoimpuritiesanddefectsenrichedinthesesites,asindicatedbyenergydispersiveX-rayspectroscopy.

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简介：Awoofer–tweeteradaptiveopticalstructuredilluminationmicroscope(AOSIM)ispresented.Bycombiningalow-spatial-frequencylarge-strokedeformablemirror(woofer)withahigh-spatial-frequencylow-strokedeformablemirror(tweeter),weareabletoremovebothlarge-amplitudeandhigh-orderaberrations.Inaddition,usingthestructuredilluminationmethod,ascomparedtowidefieldmicroscopy,theAOSIMcanaccomplishhighresolutionimagingandpossessesbettersectioningcapability.TheAOSIMwastestedbycorrectingalargeaberrationfromatriallensintheconjugateplaneofthemicroscopeobjectiveaperture.TheexperimentalresultsshowthattheAOSIMhasapointspreadfunctionwithanFWHMthatis140nmwide(usingawaterimmersionobjectivelenswithNA=1.1)aftercorrectingalargeaberration(5.9μmpeak-to-valleywavefronterrorwith2.05μmRMSaberration).Afterstructuredlightilluminationisapplied,theresultsshowthatweareabletoresolvetwobeadsthatareseparatedby145nm,1.62×belowthediffractionlimitof235nm.Furthermore,wedemonstratetheapplicationoftheAOSIMinthefieldofbioimaging.Thesampleunderinvestigationwasagreen-fluorescentprotein-labeledDrosophilaembryo.Theaberrationsfromtherefractiveindexmismatchbetweenthemicroscopeobjective,theimmersionfluid,thecoverslip,andthesampleitselfarewellcorrected.UsingAOSIMwewereabletoincreasetheSNRforourDrosophilaembryosampleby5×.

简介：Anovelfluorescencelifetimeimagingmicroscopy(FLIM)workingwithdeepUV240–280nmwavelengthexcitationshasbeendeveloped.UV-FLIMisusedformeasurementofdefect-relatedfluorescenceanditschangesuponannealingfromfemtosecondlaser-inducedmodificationsinfusedsilica.ThisFLIMtechniquecanbeusedwithmicrofluidicandbiosamplestocharacterizetemporalcharacteristicsoffluorescenceuponUVexcitation,acapabilityeasilyaddedtoastandardmicroscope-basedFLIM.UV-FLIMwastestedtoshowannealingofthedefectsinducedbysilicastructuringwithultrashortlaserpulses.Frequency-domainfluorescencemeasurementswereconvertedintothetimedomaintoextractlongfluorescencelifetimesfromdefectsinsilica.

简介：Thesurfacemorphologyoffree-surfacePCLringedspheruliteswasinvestigatedbyusingatomicforcemicroscopy.ThespheruliteswereobtainedbycrystallizationofPCL/PVCblendsofdifferentcompositions.Itwasfoundthattheringedspheruliteexhibitedregularlyfluctuatingringsonitssurface.Comparedwiththebright-darkringpatternofthespheruliteunderapolarizingmicroscope,itwasprovedthattheopticalcharacteristicsoftheringedspheruliteunderpolarizingmicroscopecoincidedwithitssurfacecharacteristics.Thebrightringsinpolarizingmicrographsofthespherulitecoincidedwiththeconvexringsonitssurface,whilethedarkringscoincidedwiththeconcaverings.

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简介：Wepresentadigitalholographicmicroscopewhereinthesampleisilluminatedbystructuredlighttoenablethecaptureofadditionalobjectspatialfrequencies.ReconstructedimageswithincreasedspatialresolutionareobtainedbyseparatingandsynthesizingbandwidthsofdifferentfrequencyregionsintheFourierdomain.Thetheoreticalanalysisandexperimentalresultsarepresented.

简介：我们调查了用原子力量显微镜学(AFM)在中国使用的三沥青(SK，Karamay，和Esso)的微观结构形态学。地形学和阶段对比图象被获得。地志的侧面和三幅维的图象被描述。微观结构的粗糙是计算的。并且沥青的化学作文被测试解释沥青的微结构机制。结果证明在原子力量显微镜学的地形学和阶段图象是适当的评估沥青文件夹的微观结构。在包括为在这研究的三沥青的像蜜蜂的结构，地志的侧面，3D图象，和粗糙的微结构形态学有重要差别。在沥青文件夹的微观结构有三个不同阶段。油来源和沥青的化学作文，特别沥青质内容在微观结构上有大影响。

简介：Opticalaxialscanningisessentialprocesstoobtain3Dinformationofbiologicalspecimens.Torealizeopticalaxialscanningwithoutmoving,thetunablelensisasolution.However,theconventionaltunablelensesusuallyinducenon-uniformmagnificationandresolutionissues.Inthispaper,wereportamovableelectrowettingoptofluidiclens.Unliketheconventionaltunablelens,ourproposedoptofluidiclenshastwoliquid-liquid(L-L)interfaces,whichcanmoveinthecellbyanexternalvoltage.TheobjectdistanceandimagedistanceareadjustedbyshiftingtheL-Linterfaceposition.Therefore,theproposedlenscanrealizeopticalaxialscanningwithuniformmagnificationandresolutioninmicroscopy.Toprovetheconcept,wefabricateanoptofluidiclensanduseitinopticalaxialscanning.Thescanningdistanceismorethan1mmwithuniformmagnificationandgoodimagingquality.Widespreadapplicationofsuchanewadaptivezoomlensisforeseeable.

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简介：AIMTo评估穿二种不同类型的新keratoconus病人的角膜的房间形态学僵硬气体可渗透(RGP)为1y.METHODSThirty的隐形眼镜39个新keratoconus病人的九只眼睛与二被选择并且随机适合RGP隐形眼镜的类型。组1有21只眼睛与常规僵硬气体可渗透(RRGP)18看的接触透镜和休息在与的组2特殊设计了僵硬气体可渗透(SRGP)接触透镜。角膜的房间形态学用在没有透镜扫描共焦的显微镜的一个裂缝被评估穿并且在接触透镜在组1穿的接触透镜wearing.RESULTSAfter1y的1y以后，吝啬的前面、以后的stromalkeratocyte密度是显著地少些(P=0.006和P=0.001，分别地)与没有透镜相比穿。前面、以后的stromalkeratocyte的吝啬的房间区域也是显著地不同的(P=0.005和P=0.001)从没有透镜穿。前面、以后的stromal薄雾增加了18.74%和23.81%，分别地在接触透镜穿的1y以后。而在组2，统计上重要的变化仅仅在房间密度与前面的基质的区域被观察(P=0.001和P=0.001，分别地)在1y以后。当时，前面、以后的stromal薄雾的水平在接触透镜穿的1y以后增加了16.67%和11.11%。Polymegathism和多形性也在接触透镜groups.CONCLUSIONConfocal显微镜学观察显示出的接触透镜在两个穿的1y以后增加了在在特别穿隐形眼镜的keratoconic角膜的角膜的房间形态学的重要改变组织1。接触透镜的类型必须小心地被选择在角膜的房间形态学最小化变化。

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简介：一个理论模型被建议以便在s-SNOM学习配置回声。在样品和探查尖端之间的电磁的联合与伪静电的领域理论被描述。这个方法允许我们分析在探查尖端和样品之间的相互作用直觉地在s-SNOM唤起的configurational回声。为某个系统的反响的条件在一张明确的表格被介绍。在考虑样品偶极子和探查偶极子的实际尺寸的条件上，我们讨论为各种各样的材料样品认识到configurational回声的可能性。，数字结果显示绝缘的探查尖端的polarizability太小，不能引起这回声，表面电浆子回声出现在金属性的nanoparticles上，金属性的探查尖端的提高的polarizability保证完成回声的要求。CLC数字TH742这个工作被中国的国家自然科学基础在资助号码下面支持30170276。

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简介：获得supercontinuum(SC)的一个新方法为散布的复合协调反司烧拉曼(汽车)的来源显微镜学被建议。在有在800.9nm的中央波长的femtosecond脉搏激光的photonic水晶纤维(PCF)的非线性的繁殖与分级的波浪理论被学习。基于事件激光力量和PCF的分散，为复合汽车显微镜学的超级宽带来源被设计。

简介：Objective:Thepresentstudyaimedtoevaluatethepossibilityofusingcoherentanti-StokesRamanspectroscopy（CARS）microscopytodeterminethespecificmolecularmorphologyofcholesteatomabydetectingthenaturalvibrationalcontrastofthechemicalbondswithoutanystaining.Materialsandmethods:SpecimensfromthemastoidandtympanicmembranewithandwithoutcholesteatomawereanalyzedusingCARSmicroscopy,two-photonexcitedfluorescence（TPEF）microscopy,andthesecondharmonicgeneration（SHG）microscopy.Results:Incholesteatomatissuesfromthemastoid,astrongresonantsignalat2845cm-1wasobservedbyCARS,whichindicatedthedetectionoftheCH2hydro-carbonlipidbondsthatdonotgeneratevisiblesignalsat2940cm-1suggestiveofCH3bondsinaminoacids.Astrongresonantsignalat2940cm-1appearedinanareaofthesamespecimen,whichalsogeneratedabundantsignalsbyTPEFandSHGmicroscopyat817nm,whichwassuggestiveofcollagen.Inthetympanicmembranespecimenwithcholesteatoma,astrongresonantsignalwithcorrugatedmorphologywasdetected,whichindicatedthepresenceoflipids.AstrongsignalwasdetectedinthetympanicmembranewithchronicotitismediausingTPEF/SHGat817nm,whichindicatedcollagenenrichment.TheCARSandTPEF/SHGimageswereinaccordancewiththehistologyresults.Conclusion:TheseresultssuggesttheneedtodevelopanovelCARSmicroendoscopethatcanbeusedincombinationwithTPEF/SHGtodistinguishcholesteatomafrominflammatorytissues.

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简介：Stimulatedemissiondepletion(STED)microscopyisoneoffar-fieldopticalmicroscopytechniquesthatcanprovidesub-diffractionspatialresolution.ThespatialresolutionoftheSTEDmicroscopyisdeterminedbythespeciallyengineeredbeamprofileofthedepletionbeamanditspower.However,thebeamprofileofthedepletionbeammaybedistortedduetoaberrationsofopticalsystemsandinhomogeneityofaspecimen’sopticalproperties,resultinginacompromisedspatialresolution.Thesituationgetsdeterioratedwhenthicksamplesareimaged.Intheworstcase,theseveredistortionofthedepletionbeamprofilemaycausecompletelossofthesuperresolutioneffectnomatterhowmuchdepletionpowerisappliedtospecimens.Previouslyseveraladaptiveopticsapproacheshavebeenexploredtocompensateaberrationsofsystemsandspecimens.However,itisdifficulttocorrectthecomplicatedhigh-orderopticalaberrationsofspecimens.Inthisreport,wedemonstratethatthecomplicateddistortedwavefrontfromathickphantomsamplecanbemeasuredbyusingthecoherentopticaladaptivetechnique.Thefullcorrectioncaneffectivelymaintainandimprovespatialresolutioninimagingthicksamples.