简介：Identificationofhigh-yieldingstablepromisingricelinesanddeterminationofsuitableareasforricelineswouldbedonebyadditivemaineffectsandmultiplicativeinteraction(AMMI)model.SevenpromisingricegenotypesplustwocheckvarietiesShiroudiand843wereanalyzedusingarandomizedcompleteblockdesignwiththreereplicationsinthreeconsecutiveyears(2012,2013and2014).Homogenouserrorvariancewasindicatedinthenineenvironmentsforgrainyield.Thecombinedanalysisofvarianceindicatedsignificanteffectsofenvironment,genotypeandgenotype×environment(GE)interactionsongrainyield.ThesignificanteffectofGEinteractionreflectedonthedifferentialresponseofgenotypesinvariousenvironmentsanddemonstratedthatGEinteractionhadremarkableeffectongenotypicperformanceindifferentenvironments.TheapplicationofAMMImodelforpartitioningtheGEinteractioneffectsshowedthatonlythefirsttwotermsofAMMIweresignificantbasedonGollob'sF-test.ThelowestAMMI-1wasobservedforG7,G2andG6.G7andG6hadhighergrainyield.Accordingtothefirsteigenvalue,whichbenefitsonlythefirstinteractionprincipalcomponentscores,G1,G6,G2andG9werethemoststablegenotypes.Thevaluesofthesumoffirsttwointeractionprincipalcomponentscorescouldbeusefulinidentifyinggenotypestability,andG6,G5andG2werethemostdynamicstablegenotypes.AMMIstabilityvalueintroducedG6asthemoststableone.AccordingtoAMMIbiplotview,G6washighyieldingandhighlystablegenotype.Inconclusion,thisstudyrevealedthatGEinteractionswereanimportantsourceofriceyieldvariation,anditsAMMIbiplotswereforcefulforvisualizingtheresponseofgenotypestoenvironments.

简介：Agronomictraitsareimportantdeterminantstoriceyield,whicharecontrolledbycomplexgeneticfactorsaswellasgenotypebyenvironment(G×E)interactioneffects.TheG×Eeffectsforagronomictraitsofricehavebeendissectedwithvariousapproaches,butnotwiththecurrentavailableapproach,theassociationstudies.Inthisstudy,atotalof32655singlenucleotidepolymorphismswereusedtocarryoutassociationswith14agronomictraitsamong20riceaccessionsintwoenvironments.TheG×Einteractioneffectsforalltheagronomictraitswereathighlysignificantlevels(P<0.01),accountingfor3.4%–22.3%ofthetotalsumofsquaresexceptforthelengthofbrownrice.Twentythreeputativequantitativetraitloci(QTLs),includingfivepreviouslyknownandseveralnewpromisingassociations,wereidentifiedfor10of14traits.AnalysisoftherelationshipsbetweenthetraitsforwhichQTLsandthegenotypeeffectscouldbeidentifiedsuggestedthatthehigherthegenotypiceffect,thehigherthepossibilitytoidentifyQTLsforthegiventrait.ThenewQTLsdetectedinthisstudywillfacilitatedissectionofthecomplexagronomictraitsandmaygiveinsightintotheG×Eeffectswithassociationmapping.

简介：Objective:TodirectionallyclonetheomplgenefromChlamydiatrachomatis(Ct)FGenotypeontoaplasmidvectorforconstructingarudimentaryDNAvaccine.Methods:ThecompleteomplgenefromgenomicDNAofCtFgenotypewildspecieswasamplifiedwithprimersdesignedbycomputer.Therecombinantgenewasobtainedbyrestrictionenzymecutting,linkingthegenewiththeplasmidvectorinvitro,transformingtherecombinantgeneintobacteria,andextractingtheDNAfromthebacteria.Results:DNAextractingfromthebacteriawascomposedoftheimplgeneandplasmid,whichisidentifiedbythreemethodsofsingularrestrictiveenzymecutting,doublerestrictiveenzymecuttingandPCR.Conclusion:CloningoftheomplgenefromtheCtFgenotypemeansthatarudimentaryDNAvaccinewassuccessfullyconstructed.

简介：Inordertoselectasuitablegenotypeforthestudiesongenetictransformation,thedifferenceamongthreegenotypes(DN04-18,DN04-25andDN04-26)ofPopulus×euramericana(Populusdeltoides×P.nigra)intermsofshootregenerationwasinvestigatedinthispaper.AnefficientinvitromicropropagationmethodwassuccessfullyestablishedforgenotypeDN04-18.Thehighfrequencyshootregeneration(90.0%)fromleafdiscswasobtainedingenotypeDN04-18onthemodifiedMurashigeandSkoog(mMS)mediumw...

简介：十四个米饭变化的长叶的率在营养生长阶段在一个播种时间实验，和快速的长叶遗传型被测量在indica变化Yanhui559被发现。Yanhui559的主要灰煤杆上的叶数字总是是多于Lemont的4-5叶子，并且Yanhui559的长叶的率基于从播种到出发的类似的生长持续时间比Lemont的显著地高。而且，长叶的率的差别在营养生长阶段是重要的，然而并非在圆锥花序开始阶段特殊。在Yanhui559和Lemont的二张回交人口的长叶的率的Gerietic分析证明那个学生和数量基因控制了快速的长叶特性的表示。每植物在backcrossing人口，更早展出到ering为止的快速的长叶遗传型和更多的tiller与类似的生长持续时间基于一些植物的调查的结果，并且它然后每圆锥花序每谷物的植物和数字包括圆锥花序的棕土的产量构成因素比慢长叶遗传型的那些优异。在繁殖的快速的长叶遗传型的进一步的研究和申请可行性被讨论。

简介：Toinvestigatetheprevalenceandgenotypeofextendedspectrumbeta-lactamases(ESBLs)mediatedbyplasmidinGram-negativebacteriafoundinsouthernChina,atotalof1184clinicalisolatesofnon-repetitivestrainsofGram-negativebacteriawerecollectedin2001from5differentcitiesinsouthernChina.TheESBLs-producingisolatesweredistinguishedbymeansofthephenotypeconfimatorytestbasedontheNCCLScriteriaandweresubjectedtoplasmidconjugationandelectroporationexperiments.Thoseclinicalisolatessucceededinplasmidtransfershadundergoneplasmidconjugationandelectro-transformation,plasmidDNAextractionandPstⅠdigestlinger-printinganalysis,aswellasthetmiversalprimerPCRamplificationoftheTEM,SHV,CTX-M,VEB,PERandSFOgenesandtheDNAsequencinginordertodeterminethegenotypesofESBLsandtheirplasmidlocations.ItwasfoundthattheincidenceoftheESBLs-producingstrainsofGram-negativebacteriawas14.6%(173/1184)with67strainsoftransconjugantsand11strainsofelectro-transformants,inwhichCTX-M-14typewas33.3%(26/78);CTX-M-3typewas23.1%(18/78);CTX-M-9typewas14.1%(11/78);CTX-M-5typewas6.4%(5/78);CTX-M-13typewas2.6%(2/78);SHV-5typewas7.7%(6/78);SHV-12typewas5.1%(4/78),SHV-2atypewas2.6%(2/78)andunidentifiedtypewas5.1%(4/78).29.5%ofthewildstrainsalsocarriedbroad-spectrumbeta-lactamasesTEM-1andSHV-1types.TheabovementionedESBLsgeneswerelocatedontransferableplasmidswithvariablesizes(from35to190kb).TheCTX-MtypeESBLswascharacterizedbyhigh-levelofresistancetocefotaxime.ItconcludedthattheCTX-M-typewasthemostprevalentgenotypeinclinicalisolatesofGram-negativebacteriainsouthernChina,andtheSHVtyperanksinthesecondplace.TEM-,VEB-,Toho-andPER-typeswerenotfoundintheseisolates.

简介：单个核苷酸多型性(SNP)是决定任何二个无关的个人之间的差别的基因变化。各种各样的人口组能用SNP与对方被区分开来。例如，HapMap数据集与大约1000万SNP有四个人口组。为人的进化，种族变化，和人口赋值上的更多的卓见，我们建议发现哪个SNP在决定人口组是重要的然后作为输入特征用这些相关SNP分类不同人口。在这研究，我们开发了评价措施的修改t测试并且把它用于HapMap遗传型数据。第一，我们为赋值包括F统计和增进知识的海角与另外的特征重要性措施比较评价所有SNP。第二，我们作为输入选择最高度评价的SNP的不同数字到一个分类器，例如支持向量机器，以便发现最好的特征相应于最好的分类精确性的子集。试验性的结果证明建议方法在发现在决定人口组是重要的SNP是很有效的，与减少的计算负担和更好的分类精确性。

简介：AbstractBackground:Hepatitis C virus (HCV) genotype 3, particularly subtype 3b, is increasing in prevalence and distribution in China. This study evaluated the prevalence, regional distribution, clinical characteristics, host factors, treatment outcomes, and disease progression of patients with HCV genotype 3 in China.Methods:A 5-year follow-up was preceded by a cross-sectional study. Treatment choices were at the discretion of treating physicians. Estimated infection time to overall-disease-progression (defined by ≥1 of: newly diagnosed cirrhosis; cirrhosis at baseline, Child-Turcotte-Pugh score increased 2 points or more; progression from compensated cirrhosis to decompensated cirrhosis; hepatocellular carcinoma; liver transplantation; or death) was calculated using the Kaplan-Meier method. Cox regression analyses were conducted to evaluate the risk factors for disease progression.Results:The cross-sectional study enrolled 997 patients, including 91 with HCV genotype 3 infection. Among them, subtype 3b (57.1%) was more dominant than subtype 3a (38.5%). Five hundred and twelve patients were included into the follow-up phase. Among patients analyzed for estimated infection time to overall-disease-progression, 52/304 (17.1%) patients with HCV genotype 1 and 4/41 (9.8%) with HCV genotype 3 (4/26 with genotype 3b, 0/13 with genotype 3a, and 0/2 with undefined subtype of genotype 3) experienced overall-disease-progression. Patients with HCV genotype 3 were younger than those with genotype 1 (mean age: 39.5 ± 8.7 vs. 46.9 ± 13.6 years) and demonstrated more rapid disease progression (mean estimated infection time to overall-disease-progression 27.1 vs. 35.6 years).Conclusions:HCV genotype 3, specifically subtype 3b, is associated with more rapid progression of liver disease. Further analysis to compare HCV subtype 3a and 3b is needed in high prevalence regions.Trial registration:NCT01293279, https://clinicaltrials.gov/ct2/show/NCT01293279; NCT01594554, https://clinicaltrials.gov/ct2/show/NCT01594554.

简介：ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.

简介：High-throughputSNPgenotypingplatformsuseautomatedgenotypecallingalgo-rithmstoassigngenotypes.Whilethesealgorithmsworkefficientlyforindividualplatforms,theyarenotcompatiblewithotherplatforms,andhaveindividualbiasesthatresultinmissedgenotypecalls.HerewepresentdataontheuseofasecondcomplementarySNPgenotypeclusteringalgorithm.ThealgorithmwasoriginallydesignedforindividualfluorescentSNPgenotypingassays,andhasbeenopti-mizedtopermittheclusteringoflargedatasetsgeneratedfromcustom-designedAffymetrixSNPpanels.Inananalysisofdatafroma3Karraygenotypedon1,560samples,theadditionalanalysisincreasedtheoverallnumberofgenotypesbyover45,000,significantlyimprovingthecompletenessoftheexperimentaldata.Thisanalysissuggeststhattheuseofmultiplegenotypecallingalgorithmsmaybead-visableinhigh-throughputSNPgenotypingexperiments.ThesoftwareiswritteninPerlandisavailablefromthecorrespondingauthor.

简介：Wereportacompletegenomicsequenceofrareisolates(minorgenotype)oftheSARS-CoVfromSARSpatientsinGuangdong,China,wherethefirstfewcasesemerged.Themoststrikingdiscoveryfromtheisolateisanextra29-nucleotidesequencelocatedatthenucleotidepositionsbetween27,863and27,864(referredtothecompletesequenceofBJ01)withinanoverlappedregioncomposedofBGI-PUP5(BGI-postulateduncharacterizedprotein5)andBGI-PUP6upstreamoftheN(nucleocapsid)protein.Thediscoveryofthisminorgenotype,GD-Ins29,suggestsasignificantgeneticeventanddifferentiatesitfromthepreviouslyre-portedgenotype,thedominantformamongallsequencedSARS-CoVisolates.A17-ntsegmentofthisextrasequenceisidenticaltoasegmentofthesamesizeintwohumanmRNAsequencesthatmayinterferewithviralgenomereplicationandtranscriptioninthecytosoloftheinfectedcells.Itprovidesanewavenuefortheexplorationofthevirus-hostinteractioninviralevolution,hostpathogenesis,andvaccinedevelopment.

简介：AbstractBackground:Patients carrying the HongKongαα (HKαα) allele and -α3.7/αααanti-4.2 could be misdiagnosed as -α3.7/αα by the current conventional thalassemia detection methods, leading to inaccurate genetic counseling and an incorrect prenatal diagnosis. This study was aimed to accurately analyze the genotypes of HKαα carriers and -α3.7/αααanti-4.2.Methods:Samples were collected in our hospital from July 2017 to October 2019. Twenty-four common types of Chinese thalassemia were screened by gap-polymerase chain reaction (Gap-PCR) and reverse dot blot (RDB). Anti-4.2 multiplex-PCR was used to confirm carriers of the αααanti-4.2 duplication with -α3.7 deletion. Two-round nested PCR and multiplex ligation-dependent probe amplification (MLPA) were applied to accurately identify and confirm their genotypes. For data analysis, we used descriptive statistics and Fisher’s exact tests.Results:Two thousand five hundred and forty-four cases were identified as thalassemia in 5488 peripheral blood samples. The results showed that α, β, and αβ compound thalassemia were identified in 1190 (46.78%), 1286 (50.55%), and 68 (2.67%) cases, respectively. A total of 227 samples from thalassemia patients were identified as -α3.7/αα by Gap-PCR, and the genotypes of two samples were uncertain. There was a difference between Gap-PCR and combined groups (Gap-PCR combined with nested PCR and MLPA) in detecting HKαα (P < 0.05). Among the 229 patients, 20 patients were identified as HKαα carriers and one was identified as -α3.7/αααanti-4.2 by two-round nested PCR and MLPA, including 15 patients with HKαα/αα, three with HKαα/αα and β-thalassemia coinheritance, one with HKαα/-SEA, one with HKαα/-α4.2 and β-thalassemia coinheritance, and one with -α3.7/αααanti-4.2 and β-thalassemia coinheritance.Conclusions:αααanti-4.2 and HKαα genotypes of patients carrying -α3.7 need to be detected to reduce the misdiagnosis rate of patients carrying HKαα and -α3.7/αααanti-4.2 alleles. More accurate genetic counseling can be provided in the clinic using nested PCR combined with MLPA.

简介：AbstractPurpose:Evidence suggests that the oxytocin receptor (OXTR) gene may be involved in the psychopathology of posttraumatic stress disorder (PTSD). This study aimed to investigate the effects of OXTR rs53576 genotype on PTSD symptoms introduced in the Diagnostic and Statistical Manual, Fifth Edition (DSM-5).Methods:This study was a cross-sectional study conducted among 1140 adults who had personally experienced the Wenchuan earthquake. PTSD symptoms were measured with the PTSD checklist for DSM-5. A custom-by-design 2 × 48-Plex SNPscanTM Kit were used to determine the OXTR rs53576. Multiple regression models were used to analyze the independent and interactive effects of OXTR rs53576 genotype and earthquake exposure on the severity of total PTSD symptoms and different dimensions of PTSD symptoms.Results:The results revealed that the rs53576 genotype could significantly predict PTSD symptoms (β = 0.055, p = 0.045). Further analysis showed that the rs53576 genotype was only significantly associated with dysphoric arousal symptoms of PTSD (β = 0.080, p = 0.005). The rs53576 genotype × earthquake exposure interaction had no significant effect on different symptom clusters (p > 0.05).Conclusion:This study showed that the rs53576 genotype was only associated with the dysphoric arousal symptoms but not with other symptom clusters of PTSD. These findings support the role of the OXTR on the psychopathology of PTSD and help us to understand the genetic basis of PTSD.

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