简介：AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.

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简介：水可分散的银nanoparticles(AgNps)用thiacalix被准备[4]arenetetrahydrazide(TCTH)作为一个减少并且稳定的代理人。TCTH-AgNps被表面电浆子回声(SPR)描绘，传播电子显微镜学(TEM)和精力散X光检查(EDX)。相对一致20?nmTCTH-AgNps的球形的粒子高效地在5-9并且从10-40的一个pH范围上被形成??甠瑬慲潳楮慣楴湯???€敆？？

简介：一篇小说溶剂敏感荧光灯有可逆性的致动器从碳点(CD)被获得了反的蛋白石，经由渗入CD答案进胶体的水晶模板的空隙被准备，CD材料的热聚合并且移开胶体的模板。同样准备的CD反的蛋白石致动器显示出75畭s的一个弯曲角度